acquity systems with 2d technology - waters …acquity systems with 2d technology ©2012 waters...

©2012 Waters Corporation 1

ACQUITY Systems with 2D Technology

MS Technology Day

Sesión Alimentación y Medio Ambiente

Barcelona, 21 de marzo de 2012


Why Bother with 2D Chromatography?


Two Dimensional Chromatography Principal

2.1

2.2

Separation Method #1

1

2

Separation Method #2

1.1

1.2


ACQUITY UPLC Systems with 2D Technology Solves Four Key Customer Challenges

Increase Selectivity & Sensitivity

Eliminate Unwanted

Interferences

Mobile Phase Flexibility

Characterize the Most Complex Samples


Agenda

Waters Two Dimensional UPLC Capability

Two Dimensional Functions and Analytical Benefits — Trapping

— Heart Cutting

— Parallel Column Regeneration

— At-Column Dilution


Simple to Use Easy to Implement Proven UPLC benefits

—Resolution —Sensitivity —Productivity

Compatible with a wide range of Chemistries

ACQUITY Systems with 2D Technology


ACQUITY UPLC Column Manager Designed for 2D Operation

Two Easy-to-access, 6-port 2-position valves Two independent temperature controlled zones Low Volume Active Pre-Heaters Ensure best

Chromatographic performance run to run and system to system


ACQUITY Systems with 2D Technology Software Control through MassLynx

Binary Solvent Manager Quaternary Solvent Manager


ACQUITY Systems with 2D Technology Software Control through MassLynx

Simple Control of Valves by Timed Events in Column Manager


Trapping


Time 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40

%

0

100

MRM of 6 Channels ES+ 327 > 269.9

1.46e6 4.31

250 uL Clozapine 1 ppb 2D – 3 min gradient


Peak distortion Volume Overload


Increase Sample Loading and Sensitivity with Trap and Transfer Configuration


Heart Cutting


ACQUITY UPLC and Xevo TQ MS Bioanalysis

1D Separation


Running 1D

1

BSM 1 +SM

BSM 2

Waste

1D column

BSM2

2D column

Trapping Column


Heart Cutting– ACQUITY UPLC with 2D Technology and Xevo TQ MS

1D Separation

2D Separation


ACQUITY UPLC with 2D Technology – Impurity Profiling Heart Cut

Heart Cut


2D Heart-cutting Benefits

Orthogonality

— In mathematics, two vectors are orthogonal if they are perpendicular, i.e., they form a right angle. The word comes from the Greek ὀρθός (orthos), meaning "straight", and γωνία (gonia), meaning "angle". For example, a subway and the street above, although they do not physically intersect, are orthogonal if they cross at a right angle.

Specificity of a method

Enhanced sensitivity in MS by dramatic reduction of matrix effect.

Possibility to load more and refocused the 1D into the 2D

http://en.wikipedia.org/wiki/Mathematics�

http://en.wikipedia.org/wiki/Vectors�

http://en.wikipedia.org/wiki/Perpendicular�

http://en.wikipedia.org/wiki/Greek_language�


1D Chromatogram

Connect Waste tubing to MS

Leave the valve in position 1 to see the 1D peak eluting

This is the 1D peak we will have to trap


Separation is performed on the 2D column


2D on the Xevo TQ-S 5pg/ml Plasma Estradiol


1D vs 2D

1D

2D


Parallel Column Regeneration


Parallel Column Regeneration

Increase Overall Speed of Analysis — Analytical Separation Performed while 2nd Column is Regenerated


Parallel Column Regeneration Case Study

Complex Samples Requiring Long Run times of 35 minutes

Regeneration and Requilibration requiring 15 minutes

24 Hour Operation Samples Run 1 Dimensional ACQUITY UPLC 28 ACQUITY UPLC with 2D Technology 41

30% Increase in Throughput


Summary – ACQUITY Systems with 2D Technology

Simple to Use

Easy to Implement

Straightforward to operate in various 2D functions — Trapping

— Heart Cutting

— Parallel Column Regeneration

— At-Column Dilution

acquity systems with 2d technology - waters …acquity systems with 2d technology ©2012 waters...

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