acquire ovarian cancer cell lines from various repository
DESCRIPTION
Supplementary Figure 1. A. Acquire ovarian cancer cell lines from various repository ATCC (6), CRT (2), DSMZ (2), ECACC (7), Kyoto U (24), RIKEN (1). Establish EM Phenotypic Characterisation. Validate EMT Marker Expression. Cell line expansion & banking SGOCL (43). Create EMT Spectrum. - PowerPoint PPT PresentationTRANSCRIPT
AAcquire ovarian cancer cell lines from
various repositoryATCC (6), CRT (2), DSMZ (2), ECACC
(7), Kyoto U (24), RIKEN (1)
Cell line expansion & bankingSGOCL (43)
Downstream Analysis
Immunofluorescence StainingDNA, RNA, Protein Extraction
BRNA
Gene Expression Profiling
Phenotype-specificGene Signatures
EMT RT2 Profiler qPCR Array
Phenotype-specificGene Expression
Affymetrix GeneChip Human Gene 1.0 ST Array
QA Agilent Bioanalyzer
Parametric / NonparametricAnalysis
Cell
Functional Assays
Migration AssayInvasion Assay
Anoikis AssayColony Assay
Endpoint ReadoutImage Analysis
Establish EM Phenotypic Characterisation
Validate EMT Marker Expression
Create EMT Spectrum
Functional Correlation
Supplementary Figure 1
Supplementary Figure 2
Supplementary Figure 3
A
Caov3 OVCAR-3PEO1 OVCAR8
OVCA433IGROV1 PEO4OVCA429
CH1 SKOV3DOV13 Hey C2
A2780 Hey A8A1847 TykNu
B
Caov3
IGROV1 OVCA433 OVCA429
CH1 DOV13 SKOV3 Hey C2
A1847
PEO1 OVCAR3 OVCAR8
A2780 Hey A8 TykNu
Migration Invasion
C D E
OV
CA
433
Hey
C2
Ep
ith
elia
lIn
term
edia
teE
Inte
rmed
iate
MM
esen
chy
mal
Invasive Front
F
SKOV3 subcutaneous tumours Day 59
SKOV3 subcutaneous tumour. Measurements
in centimeters (cm)
Control 100 nM 5 MA
ZD
0530
OV
CA
R3
(E)
500 nMA
ZD
0530
OV
CA
433
(In
t E
)
AZ
D05
30
SK
OV
3 (I
nt
M)
AZ
D05
30
OV
CA
R10
(M)
Supplementary Figure 4
Supplementary Figure 5
A B
Supplementary Figure 6
A
B
Supplementary Figure 71-yr PFS 2-yr PFS 3-yr PFS 4-yr PFSA
B
Phenotype Comparison
Progression-free Survival (PFS)p-value (Log-rank)
1-yr 2-yr 3-yr 4-yrE vs IE 0.079 0.691 0.7625 0.4864E vs IM 0.21 0.163 0.1822 0.2253E vs M 0.0014 0.0002 0.0024 0.007
IE vs IM 0.9636 0.3289 0.1544 0.1043IE vs M 0.1538 0.0036 0.0053 0.0041IM vs M 0.2978 0.2209 0.4282 0.5109
CPhenotype
Comparison
Difference in RMST (95% CI)
12 month 24 month 36 month 48 month
E vs IE 0.468 (0.346 - 0.589) 0.979 (0.813 - 1.145) 0.034 (-0.125 - 0.192) -1.96 (-2.101 - -1.819)E vs IM 0.487 (0.370 - 0.604) 2.311 (2.121 - 2.501) 3.362 (3.18 - 3.544) 4.173 (4.007 - 4.338)E vs M 1.011 (0.875 - 1.147) 4.155 (3.95 - 4.36) 5.919 (5.731 - 6.106) 6.542 (6.378 - 6.705)
IE vs IM 0.019 (-0.129 - 0.167) 1.332 (1.141 - 1.524) 3.328 (3.159 - 3.497) 6.133 (5.987 - 6.278)IE vs M 0.543 (0.376 - 0.71) 3.176 (2.969 - 3.382) 5.885 (5.711 - 6.059) 8.502 (8.358 - 8.645)IM vs M 0.524 (0.362 - 0.686) 1.844 (1.614 - 2.074) 2.557 (2.359 - 2.754) 2.369 (2.201 - 2.537)
Supplementary Figure 8
E-cad/DAPI N-Cad/DAPI