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Analysis of chemical composition of volatile substances from two
tobacco leaf species by headspace solid-phase microextraction (HS-
SPME) coupled with gas chromatography-mass spectrometry (GC-
MS) techniques was explored. The optimum conditions were the
extraction of tobacco leaf (1.000 gram) with DVB / CAR / PDMS for 30
minutes at a 50 degree Celsius. Thirty-six compounds from Burley
tobacco was found of which 22 signals were identified representing
peak for 90.31 %. The results showed that pyridine was the main
component representing 70.98 %. Chemical composition from
Virginia tobacco was found with 20 signals. Fifteen signals
representing 90.63 % were identified. The results showed that 6-
methyl-5-hepten-2-one was the main component Virginia tobacco
representing 27.84 %.
The cigarette manufacturing industry in Thailand, divided into 3
types, which are Virginia, Burley and Turkish leaves.
When the tobacco burned, will cause different chemical composition
and other properties such as smell and taste, which vary according
to the type of tobacco.
The industry Producing cigarettes is necessary to mix different
types of tobacco leaves together proportionally in order to get the
flavors satisfied by the smoker.
Analysis of chemical composition of volatile substances from two tobacco leaf species (Nicotiana
tabacum L.) by headspace solid-phase microextraction coupled with gas chromatography-mass
spectrometry techniques
ABSTRACT
INTRODUCTION
METHODOLOGY
Headspace vial1.000 g tobaccoWater bath
In 50°C for 60 mins
Saturate
SPME
AbsorbDesorb
For 30 minsEvaluate by
chromatography
GC-MS
Find the optimal condition for extraction of Burley and Virginia tobacco
RESULT AND DISCUSSION
Neophytadiene
Figure 1 The chromatogram show the ratio of
Neophytadiene
Pyridine
PyrazineSabinene
Neophytadiene
Figure 2 Chromatogram show volatile substances
from Burley tobacco
Figure 3 The chromatogram show volatile
substances from Virginia tobacco
Neophytadiene
6-Methyl-5-hepten-2-oneAcetic acid
Benzyl alcoholPhenylethyl alcohol
The analysis result of volatile substances from Burley tobacco showed that pyridine was the main
component representing 70.98 % and Virginia tobacco showed that 6-methyl-5-hepten-2-one was
the main component representing 27.84 %.The optimal condition for extract the volatile substances
from Burley and Virginia tobacco by HS-SPME coupled with GC-MS techniques, the temperature is
50 oC and the Absorption time is 30 minutes.
CONCLUSION
References1. Clark, T. J., & Bunch, J. E. (1997). Qualitative and Quantitative Analysis of Flavor Additives on Tobacco Products Using SPME−GC−Mass Spectroscopy. Journal of Agricultural and Food Chemistry, 45(3), 844–849.
2. Xiang, Z., Cai, K., et. al. (2013). Simultaneous Extraction and Derivatization of Volatile Organic Acids in Flue-Cured Tobacco with Headspace Solid-Phase Microextraction Followed by GC-MS. Analytical Letters, 46(4), 640–650.
We would like to thank Asst. Prof. Dr. Aphiwat Teerawutgulrag, Mr.Theerawat Sankumlor and
Ms. Chanidapha Thiraphatchotphum at Laboratory of chemistry building.
Department of chemistry,Faculty of science,Chiang Mai University
Acknowledgements
Table 1 % Area of Neophytadiene with the
temperature was 30, 40, 50 ˚C, respectively.
Graph 1 the graph show %Area of Neophytadiene
with the temperature was 30, 40, 50 ˚C,
respectively.
Table 2 % Area of Neophytadiene with the
adsorption time was 10, 20, 30 minutes,
respectively.
Graph 2 Show the graph %area of Neophytadiene
with the adsorption time was 10, 20, 30 minutes
Author : Ms. Thunyarat Boonapibal Student ID 590510045
Asst. Prof. Dr. Aphiwat Teerawutgulrag
Institution : Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200,Thailand
Neophytadiene
Neophytadiene
Neophytadiene
Neophytadiene
Analysis of chemical composition of volatile substances from Burley and Virginia
tobacco by HS-SPME coupled with GC-MS techniques
Thirty-six compounds from Burley tobacco was found
of which 22 signals were identified representing 90.31
% peak are. The results showed that pyridine was the
main component representing 70.98 %. Chemical
composition from Virginia tobacco was found with 20
signals.
Fifteen signals representing 90.63 % were identified.
The results showed that 6-methyl-5-hepten-2-one was
the main component Virginia tobacco representing
27.84 %
1. To Analysis of chemical composition of volatile substances from Burley
and Virginia tobacco by HS-SPME coupled with GC-MS techniques
2. To find The optimal condition to extract Burley and Virginia tobacco
OBJECTIVE
Table 3 the optimal condition