Udayana University

ABSTRACT BOOK

The InternationalConference on Bioscience s 2016

"Advancing Biodiversityfor Sustainable Food Securitv"

July 26 - 27 ,20163'u Floor Postgraduate Building, Udayana Univenity,

Jl, PB Sudirman, Denpasar-Bali, Indonesia

held byacultv of Mathematics and Natural Scrences.'

Postgraduate Study Program of Biology,and School of Biology, Udayana University, Bali

in collaboration with

North Dakota State University, USA

The Internatinnal Innference an Binsciences 20l6 "Advancing Biodiversity for Susta nable Food Serrrity"

IIEALTH AND MICROBIOLOGY

IAntibacterial Activity Against Stttlt/rykrcoct u.s aureu.s I r't 'tnt l\lethanc'lExtract of Mangosteen Rind (Garciniu nungostano L.)Ketut Widyani Astuti and Ni Putu Ayu Dewi Wijayanti

JJ

2

Antagonistic Activity of Salucca zalaccu Peel Against Sk4thyloutccusaureus ATCC 2592.3 and Methicillin-Resis tance .Stoph.tloto((t!.r uureu,tATCC 3359 IPutu Agastya Satryana, Gek Marlathasia Aswania, and Ni NengahDwi Fatmawati

JJ

3The Potential of Honeycomb From WildMochammad Junus

Bee3.1

4Characterization of Lactic Acid Bacteria Isolated fronr Kirnchi withAview for Development of Probiotic PotentialAgestiawan I.G.A.M. , Ramona Y,, and Swastini D.A.

3{

5

Mechanisms of Antibiotic filtrate Streptontyces tlrcrntocarboryrla.s againstFusarium oxysporum F020 Ultrasructure through Scanning FilcctronMicroscope and Transmission Electron MicroscopeRetno Kawuri

J : )

6

Detection ol Antibacterial Compound of Piper bale L. Purifled ExtractAgaints Propionibacte riunr acnes by BioautographyNi Luh Putu Vidya Paramita, Ni Wayan Budiningrum, Anak AgungGede Rai Yadnya Putra, Putu Sanna Yust iantara, and I Nlade AgusGelgel Wirasuta

36

7

Identification of Antagonists Isolated fi 'onr Rhizosphere Zone ofWatermelon Farnt at West Sanur Village Bali to Fight Fu,sariunroxysporum in f'ectionA.A. Ngurah Nara-Kusuma, Yan Ilamona, and Meitini WahyuniProborini

36

8Role of Native Mycorrhizae Gigaspora. Acaulospora and (i/olll.s sp. onthe Growth of Cashew Nut (Anacardium occidentule L.) SeedlingsMeitini Wahvuni Proborini

9Seafood Culinary Satbty: A Preliminary Study in Badung, BaliLuh Putu T. Darmayanti, N.L. Ari Yusasrini, and IDG. MayunPermana

J8

l0Microbial Contamination in Traditional Food Processing PedetanNi Made Ayu Suardani Singapurwa, A.A. Made Semariyani , andPutu Candra

38

t lAnalysis of J'otal Microbial F'resh Vegetables in Styrofbam llox With lceCool ing Techn iq uc Dur ing StorageIda Ayu Rina Prat iwi Pudia and Pande Ketut Diah Kencana

.19

l 2

Bioactivity of Antibacterial Compounds Isolated tiorn EndophyticActinomyce tcs rVeesia altissinto Bl.Rina Hidayati Pratirvi, Wibowo Mangunwardoyo, and Rosa DeryiPratiwi

39

t l Copper Hair Level on Clhildren with HIV/AIDSNiruri R, Wati K.D.K, Oktavianti K.A.D, and Cahvani NLR

40

ABSTRAIT EBI|(

The International Innference on Biosciences 20lE "Advancinq Biodilrrsitv for Sustainable Food Security"

DBTECTION OF AI{TIBACTERIAL COMPOUND OF Piper betleL. PURIFIED EXTRACT AGAINTS Propionibacterium acnesBY

BIOAUTOGRAPHY

Ni Luh Putu Vidya Paramitao, Ni Wayan Budiningrum, Anak Agung Gede RaiYadnya Putra, Putu Sanna Yustiantara, and I Made Agus Gelgel Wirasuta

t)epadment of Phannacy. Faculty of Mathematic & Science. University of Udayana*Email : putu.vidya.pararnita@grnai l.corr

ABSTRACTPntJtittnibacterium acnes are one of bacterial in skin surface which triggering an

inflammation in acne. Purif ied extrdct of Piper belle Leaf has been reported for antibacterialactivity against Propionibacterium acnes (Widyaningtyas, 2014). The aim of this study wasto dctection of antibacterial compound of P. betle L.purified extract against P. acnes 6,tbioautography method. Detection of antibacterial compound was evaluated using TLCcontact-bioautogmphy. The inhibil ion zones were observed on the agar surface in the placesrvhere the spots of antimicrobials are stuck in the agar. Screening phytochemical of theinhibit ion zone was observed using reagents ph).tochemical such as ammonia (tlavonoid).FeCls (phcnol). folin-ciocalteau (phenol), Liebermann burchard (triterpenoid and steroid).annisaldehyde-H rSOr (terpenoid). Antibacterial compound which inhibit the growth of P.dcrs.t *as showed in HRf i7 on agar surface. Phenol or derivates phenol in Piper betle wercresponsible for thc antibacterial activity of P. betle against P. acaes. The flavonoidconrpound in Purif ied extract of P. betle are not active to inhibit the growth of P. acnes.Phenol or derivates phenol in P. betle is known like eugenol, hydroxychavicol. andchavibetol. Investigation of active compound using GC-MS are needed. Altough purit it:extract of P. 6r:l/c consist the highest amount of f lavonoid, but the antibacterial compounJfor inhibit ion lhe P ,!cnes colonies were phenol or derivates phenol.

Keyrvords: l'ryter batle, Phenol, Derivates phenol, Contctct-Bioautogruphv, TLC

IDENTIFICATION OF ANTAGONISTS ISOLATED FROMRHIZOSPHERE ZONE OF WATERMELON FARM AT WEST

SANUR VILLAGE BALI TO FIGHT Fusarium oxJ,sporuntINFECTION

A.A. Ngurah Nara Kusumal , Yan Ramona l '2* , and Mei t in i Wahyun i Probor in i lrPostgraduate Study Program of Biology, Udayana Universit;

rlntegrated [-ab- fbr L]iosciences and Biotechnology. Udayana IJniversity*f.nrail: yan rarnona@yahoo.com or yan ramona(iunud.ac.ic

ABSTRACTFtrsoriurn o\.r 'slorum is a tungal pathogen that causcs severe diseases in many

horticultural crops. including rvater melon. In Sanur area where water melon has beencultivated fbr years. this fungal pathogen has cause signil icant loss among famrers. LJnti lreccntly. fanners relv on the use of chemical based lungicides to cope with this fungalinfection. To reduce negative side effects of these pesticides, thc usc of fungal antagonistshas reccntly attracted significanl atlention.

' l hercfore. our research rva-s airned to isolate and

idcnti ly rnicrobes antagonistic against .F. c.xl,sporunt(the causative agent of vasoular wilt inwatcrnelon plarrts). The antagonists rvere isolated fronr soil samples collected from

MSTRA[]BOIK t 5

[he Internalcn: l ! r r ierence on Biosciences 20lE "Advancing Bindiversi ty for Sustainable tood Securi ty"

lvatermelon larm locatcd at west Sanur vil lage, South Denpasar, Bali. Isolation andidentif ication of thc antagonists were conducted by appi_r ing spread plate and observation ofrnorphological charactcristics (fbr fungal antagonist) of biochemical reactions (for bacterialantagonists) nethods. respectively. Two fungal species (Trichoclerna har:iununt andTrichoderma tiritle) and two bacterial spccies (Pserdonorras sp. and Bacillus sp.)antagonistic against Fusarium oxysporwtand, having potentialto be dcvelopcd as biocontrolagents, were successtullv isolated in this project.

Keywords: uulrrnrrl,,rr. I r ichoderma harzianum. Trichoclennu lir idc. Pserirloirrorr.r., :;- .Bac ' t l l r i s :o . . Ba l i -

ROLE OF NATIVE MYCORRHIZAE Gigaspora, AcaulosporaAND Glomus sp. ON THE GROWTH OF CASHEW NUT

(Anacardium occidenlale L.) SEEDLINGS

Meitini Wahyuni ProboriniB\o\og1 Depanenrent. Rasic Scrence Facu\q. Univers\ of Udayana

Email: orneitini@unud.ac.id

ABSTRACT[ndornyconhiza can strategically be applied as an effort to overco[le the problerr of

agriculture at arid re-qion such as at north-east and north-west of Bali. Many plant studies atmarginal arca reponed the significant role of endomycorrhiza on boosting the groMh rate,productiyity and resistance to planl diseases nevertheless, it less concern on applied ofEndomycorrhiza in Bali. Therefore it is strategic to conduct a reserach for lndigenous Balicndomyconhiza development. The airn of this study was to formulate combining number ol(i/orzrr.r, (i igaspora and Acaulospora spores (0, 45,60.75 and 90) for ca:;hew nut nurserl(, lnacarditott occidentqle\ with three replication. The experiment was conducted at grec,,Itouse of Biology l)epartrnent Basic Science Faculty Udayana tJniversity. The resulrrshowed that there $as positive result on the growth response of A. occidentole seedliny(leaves number and plant height) by 75 number ofspores. Therefore there was no signiticanreffect on the response of.{. occidentale seedling by 90 nurnber of mycorrhizal spores. Thisfinding indicate that the optimum number of spore was 75 spores take a positive role on thegroWth ofcashew nut seedlings.

Keywortfs. cntlonyt r tt'h:u. sltctres. indigenous Bali, coshew seedlings

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By:A.A. N. Nara Kusuma, Yan Ramona, Meitini W. Proborini

Postgraduate Study Program of BiologyUdayana University

Background� Fusarium infection in water melon farm in Bali has

been so massive

� Claim as the main cause of loss in farming practices (water melon)

� According to the report of the Indonesian Statistical Agency:Agency:

� water melon production in Indonesia reached 474,327 tons in 2009

� decreased sharply in 2010 where it only reached 348,631 tons

� Mainly due to Fusarium oxysporum infection

� Until recently, water melon farmers rely on the use of chemical-based fungicides to cope with this infection

� Long run application can cause many bad side effects

� Therefore: there is a need to find more environmentally friendly methods

� Biocontrol method appeared to be the best� Biocontrol method appeared to be the best

� Research Objective:

To obtain potential antagonist isolates to be used to fight F. oxysporum infection in water melon plant.

Materials and Methods

� Isolation of antagonists

� Using dilution and spread plate method on PDA or NA

� Dual culture assay against F. oxysporum

� Identification of bacterial and fungal antagonists

� Based on morphological and physiological characteristics

� Compared with that specified in book references:

� Fungi and Food Spoilage

� Bergey’s Manual of Determinative Bacteriology

Results� Two potential fungal and two bacterial antagonists were

successfully isolated in this project. The two potential fungal antagonists were identified as Trichodermaharzianum and Trichoderma viride

Figure 1: Macroscopic and microscopic characteristics of T. harzianum(A and B) and T. viride (C and D). Their Fialid and Konidia are shown by numbers 1 and 2, respectively

� Two bacterial antagonists were successfully isolated in this research and they were identified as Pseudomonas sp. and Bacillus sp.

Figure 2: Microscopic characteristic of Pseudomonas sp. (A) and Bacillus sp. (B) observed under a light microscope (Yazumi) with magnification of 1000x

Conclusion

� Base on the morphological and physiological characteristics of the isolates, the two fungal and bacterial isolates were identified as T. harzianum, T. viride, Pseudomonas sp. and Bacillus sp.