a what happens inside? what is maxwell 16? what else can maxwell 16 do? plant tissues & seeds...
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What happens inside?What is Maxwell 16? What else can Maxwell 16 do?
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
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What happens inside? What else can Maxwell 16 do?
SpecialOffer
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
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What is Maxwell® 16?
Imagine if yourgDNA preps consisted of:•Placing a sample into a cartridge•Pushing a button•Walking away •Returning ~45 minutes later to high yield,pure gDNA ready for assay!
The Maxwell 16 System is a cartridge-based purification system bringing automation to your benchtop. Change applications by changing cartridges and selecting a different program.
Maxwell 16 can bring better results to your laboratory thanks to improvements in purity and consistency.
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What is Maxwell 16? What else can Maxwell 16 do?
SpecialOffer
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
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What happens inside?
Maxwell® 16 is a magnetic particle processor, not a liquid handler. •The sample is mixed by rapid up-and-down movement of the single-use plunger.•The MagneSil® Paramagnetic Particles are captured after a magnetic rod insert down the middle of the plunger.•The MagneSil Particles bind the nucleic acid•The nucleic acids are purified through a series of capture and release washes•The purified nucleic acid is released.
Maxwell 16® Tissue Genomic DNA Cartridge
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1 3 4 5 6 7
2 3 4 5 6 7
21 4 5 6
Mixing
Capture
Binding
Washing& Elution300µl
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What happens inside?What is Maxwell 16? What else can Maxwell 16 do?
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Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
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What else can Maxwell® do?
Low Elution Volume (LEV) gDNA
Standard Elution Volume (SEV) & LEV Total RNA
SEV Polyhistidine Protein Purification
SEV?LEV?
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What happens inside?What is Maxwell 16? What else can Maxwell 16 do?
SpecialOffer
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
Close
What else can Maxwell® do?
Low Elution Volume (LEV) gDNA
Standard Elution Volume (SEV) & LEV Total RNA
SEV Polyhistidine Protein Purification
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2 3 4 5 61
Maxwell® 16 can work with two formats of cartridges.
SEV & LEV
Standard ElutionVolume (SEV)
Low Elution Volume (LEV)
Maxwell® 16 Instrument can switch from SEV to LEV format or back in less than 5 minutes.
300-600µl
30-100µl
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What happens inside?What is Maxwell 16?
SpecialOffer
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
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Low Elution Volume gDNAProcess 1-10 5µm formalin-fixed paraffin-embedded sections after overnight proteinase K digest. No xylene extractions.
Maxwell® 16 FFPE Tissue LEV DNA Purification Kit
Cat. # AS1130
Maxwell® 16 Cell LEV DNA Purification Kit
Cat. # AS1030
Maxwell® 16 LEV Total Nucleic Acid Purification
Kit Coming Soon!
Designed for 10-10,000 cells. Ideal for capturing gDNA from samples like stem cell colonies, cerebral spinal fluid, etc.
Currently in testing for viral total nucleic acid purification
More information in the Clinical Section of http://www.maxwell16.com/
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What happens inside?What is Maxwell 16?
SpecialOffer
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
SEV & LEV Total RNAMaxwell® 16 Total RNA
Purification Kit Cat. # AS1050
Maxwell® 16 Tissue LEV Total RNA Purification Kit
Cat. # AS1220
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Maxwell® 16 Cell LEV Total RNA Purification Kit
Cat. # AS1225
Maxwell® 16 Tissue LEV
28S
18S
M 1 2 3 4 5 6
1 Brain2 Heart3 Kidney
4 Liver5 Lung6 Spleen
1µg loaded, denaturing gel
Samples are homogenized & processed to remove gDNA prior to addition to the cartridge and purification.
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What happens inside?What is Maxwell 16?
SpecialOffer
Plant Tissues& Seeds
Culturedcells & bacteria
What can Maxwell® 16 do?
SEV & LEV Total RNAMaxwell® 16
Polyhistidine Protein Purification Kit
Cat. # AS1220
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Purifies polyhistidine-tagged protein from bacteria, insect cells, mammalian cells, cell-free expression reactions, conditioned cell culture media, etc.
Total Wash Eluate
HQ-tagged MonsterGreen™ Fluorescent Protein expressed in S30 T7 High Yield Expression System
B = Mouse BrainH = Mouse HeartI = Mouse small intestineL = Mouse Liver
P = Mouse pancreasS = Mouse spleenT1 = 1cm mouse tailT0.5 = 0.5 cm mouse tail
5µl gDNA eluate
1µl gDNA eluate in PCR
Quick Isolation from a variety of tissues
Maxwell® 16 Tissue DNA
Purification Kit Cat. # AS1030
48 preps
Maxwell 16® Tissue DNA Purification Cartridge
2 3 4 5 6 7
TissueSample
Mouse tails and other animal tissueare homogenized directly in lysis buffer without Proteinase K.
gDNA from Model Organisms gDNA from Food SamplesBack
B = Mouse BrainH = Mouse HeartI = Mouse small intestineL = Mouse Liver
P = Mouse pancreasS = Mouse spleenT1 = 1cm mouse tailT0.5 = 0.5 cm mouse tail
5µl gDNA eluate
1µl gDNA eluate in PCR
Quick Isolation from a variety of tissues
Maxwell® 16 Tissue DNA
Purification Kit Cat. # AS1030
48 preps
Maxwell 16® Tissue DNA Purification Cartridge
2 3 4 5 6 7
TissueSample
Mouse tails and other animal tissueare homogenized directly in lysis buffer without Proteinase K.
Use Maxwell® 16 to speed research with Model Organisms
More information onlinehttp://www.promega.com/maxwell16/applications
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SourceYield
(PicoGreen®)Ct
(1µl eluate)Ct
(5µl eluate) Target
Drosophila melanogaster
0.32µg (1 fly)
1.52µg (5 flies)24.4 21.5
GAPDH gene
Danio rerio 24.8µg50mg tissue
26.7 24.5 p53 gene
Caenorhabditis elegans
0.08µg~50,000 whole
worms
notdetected
34.4 Laminin
Saccharomyces
cerevisiae
0.72µg3mm colony with
lyticase pre-treatment
25.3 21.5 LYS2
gDNA from Food SamplesBack
B = Mouse BrainH = Mouse HeartI = Mouse small intestineL = Mouse Liver
P = Mouse pancreasS = Mouse spleenT1 = 1cm mouse tailT0.5 = 0.5 cm mouse tail
5µl gDNA eluate
1µl gDNA eluate in PCR
Quick Isolation from a variety of tissues
Maxwell® 16 Tissue DNA
Purification Kit Cat. # AS1030
48 preps
Maxwell 16® Tissue DNA Purification Cartridge
2 3 4 5 6 7
TissueSample
Mouse tails and other animal tissueare homogenized directly in lysis buffer without Proteinase K.
gDNA from Model Organisms
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Directly process food samples to detect…
milk cheesesPanel A: S. aureus presence in milk and cheese samplesPanel B: Multiplex PCR for S. aureus toxins
…bacteria in dairy products
Amplification of 18S bovine rRNA and 16S bacterial rRNA .
…bacteria in raw meat samples
Data courtesy of P. Leone and P. Cremonesi, Italian National Council of Research-Instititute of Agro-Biology and Biotechnology
More information online:www.promega.com/maxwell16/applications
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Maxwell® 16 Blood DNA Purification Kit
Cat. # AS101048 preps
400 300 200 100 75 50
µl fresh whole blood
+4°C -20°C RTStorage Condition
ApoptoticDNA
Isolate gDNA from good or “bad” blood
Maxwell 16® Blood DNA Purification Cartridge
2 3 4 5 61
Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge
Buffy Coat Data Concordance StudyBack
Maxwell® 16 Blood DNA Purification Kit
Cat. # AS101048 preps
400 300 200 100 75 50
µl fresh whole blood
+4°C -20°C RTStorage Condition
ApoptoticDNA
Isolate gDNA from good or “bad” blood
Maxwell 16® Blood DNA Purification Cartridge
2 3 4 5 61
Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge
Concordance Study
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Using buffy coat cells gives higher gDNA yields and concentrations
Buffy CoatgDNA >100ng/µl
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Maxwell® 16 Blood DNA Purification Kit
Cat. # AS101048 preps
400 300 200 100 75 50
µl fresh whole blood
+4°C -20°C RTStorage Condition
ApoptoticDNA
Isolate gDNA from good or “bad” blood
Maxwell 16® Blood DNA Purification Cartridge
2 3 4 5 61
Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge
Buffy Coat Data
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PCR results concordant to reference extraction method
16 samples amplified, analyzed by gel electrophoresis
Data courtesy:Dr. Deborah O. Crowe, PhD, D(ABHI)
DCI Laboratory, Nashville, TN
Reference Manual Purification Method
Maxwell® 16400µl whole blood
Maxwell® 16300µl buffy coat cells
Quick gDNA isolation from cultured cells
Maxwell® 16 Cell DNA Purification Kit
Cat. # AS102048 preps
Maxwell 16® Cell DNA Purification Cartridge
2 3 4 5 61
Directly process • Cultured mammalian cells• Gram negative bacteria
Pre-treat Gram positive bacteria with
lysozyme
HeLa
1x106 cells
Designed for 1 x 105 to 5 x 106 mammalian cells
Gram NegativeBacteria
Gram Positive Bacteria
3T3CHO 293
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Quick gDNA isolation from cultured cells
Maxwell® 16 Cell DNA Purification Kit
Cat. # AS102048 preps
Maxwell 16® Cell DNA Purification Cartridge
2 3 4 5 61
Directly process • Cultured mammalian cells• Gram negative bacteria
Pre-treat Gram positive bacteria with
lysozyme
HeLa
1x106 cells
Designed for 1 x 105 to 5 x 106 mammalian cells
Gram Positive Bacteria
3T3CHO 293
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Genomic DNA isolation from a variety of Gram negative bacteria
400µl overnight culture
Escherichia coli
Pseudomonas aeruginosa
Proteus vulgaris
Salmonella typhimurium
Yersinia enterocolitica
1 2 3 4 51
2
3
4
5Designed to work with up to
2 x 109 bacterial cells
Quick gDNA isolation from cultured cells
Maxwell® 16 Cell DNA Purification Kit
Cat. # AS102048 preps
Maxwell 16® Cell DNA Purification Cartridge
2 3 4 5 61
Directly process • Cultured mammalian cells• Gram negative bacteria
Pre-treat Gram positive bacteria with
lysozyme
HeLa
1x106 cells
Designed for 1 x 105 to 5 x 106 mammalian cells
Gram NegativeBacteria
3T3CHO 293
Close
Genomic DNA isolation from a variety of Gram positive bacteria
400µl overnight culture
Staphylococcus aureus
Arthrobacter luteus
Bacillus subtilis
Bacillus cereus
Enterobacter faecalis
1 2 3 4 51
2
3
4
5Cells treated with lysozyme prior to
isolation
Designed to work with up to 2 x 109 bacterial cells
400µl overnight culture
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Maxwell® 16 Cell DNA Purification Kit
Cat. # AS102048 preps
More Plant Samples
Maxwell 16® Tissue DNA Purification Cartridge
2 3 4 5 6 7
LeafPunch
Many plant samples can be processed directly. Others may require an enzymatic pre-treatment
Improve the purity and consistency of your plant genomic DNA purification
Isolation genomic DNA from eight samples of 25mg each of tomato leaf using the Maxwell 16 Instrument and the Maxwell 16 Tissue DNA Purification Kit.
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Maxwell® 16 Cell DNA Purification Kit
Cat. # AS102048 preps
Maxwell 16® Tissue DNA Purification Cartridge
2 3 4 5 6 7
LeafPunch
Many plant samples can be processed directly. Other may require an enzymatic pre-treatment
Improve the purity and consistency of your plant genomic DNA purification
Isolation genomic DNA from eight samples of 25mg each of tomato leaf using the Maxwell 16 Instrument and the Maxwell 16 Tissue DNA Purification Kit.
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Example Yield and Purity with Maxwell® 16 Tissue DNA Purification Kit
www.promega.com/maxwell/applications
Plant Tissue Samples
1. Yields determined with PicoGreen® Reagent (Molecular Probes).2. Details available at www.promega.com/maxwell16/applications 3. Samples digested with CelluACE™ XG System (Cat. # FF3800) in the presence of polyvinylpyrrolidone prior to isolation.4. Samples digested with CelluACE™ XG System (Cat. # FF3800) prior to isolation.
Sample Size Yield1
Detectedby qPCR2
Arabidopsis thaliana (mustard)
1 leaf 139ng +
Brassica napus(canola)
5 seeds 2.3µg +
8mm punch 512ng +
Fragaria ananassa(strawberry)
8mm punch 193ng3 +
Glycine Max (soybean)
1 seed 3.1µg4 +
8mm punch 587ng +
Gossypium hirsutum
(cotton)
1 seed 2.6µg4 +
8mm punch 854ng +
Sample Size Yield1
Detectedby qPCR2
Medicago sativa(alfalfa)
8mm punch 1.4µg +
Oryza sativa(rice)
1 seed 216ng4 +
5mm punch 238ng4 +
Solanum lycopersicum(tomato)
8mm punch 734ng +
Triticum aestivum(wheat)
5mm slice 5.6µg4 +
1 seed 1.4µg4 +
Vitis vinigera (grape) 8mm punch 20ng3 +
Zea mays (corn) 8mm punch 655ng +
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Rapid Mouse Tail gDNA isolation…for less
5µl of gDNA from 8 x 1cm mouse tail snips
Discover howsimple your gDNApreps can beAsk for aDemo!
Get Maxwell 16 into your lab, so you can• Isolate tail gDNA sooner
• Genotype mice earlier
• Identify transgenics faster
All without Proteinase K!
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