a synthetic biology approach to heavy metal detection
DESCRIPTION
A Synthetic Biology Approach to Heavy Metal Detection. Eric Yang and Qiang Shi Emporia State University. Heavy Metal Poisoning. Arsenic, mercury, lead, antimone, cadmium, etc. Contamination of water and soil Oxidative stress to cells --- damage to DNA and proteins - PowerPoint PPT PresentationTRANSCRIPT
A Synthetic Biology Approach to Heavy Metal Detection
Eric Yang and Qiang ShiEmporia State University
Heavy Metal Poisoning• Arsenic, mercury, lead, antimone, cadmium, etc.• Contamination of water and soil• Oxidative stress to cells --- damage to DNA and proteins• Disrupt function in vital organs and glands• Example: Mercury Effects
– adrenal dysfunction, kidney damage, allergy, alopecia, anorexia, anxiety, birth defects, blushing, brain damage, cataracts, cerebral palsy, poor coordination / jerky movements, deafness, depression, dermatitis, discouragement, dizziness, drowsiness, eczema, emotional disturbances, excess saliva, fatigue, gum bleeding and soreness, headaches (band type), hearing loss, hyperactivity, hypothyroidism, forgetfulness, immune dysfunction, insomnia, irritability, joint pain,, loss of self-control, memory loss, mental retardation, metallic taste, migraines, nervousness, nerve fiber degeneration, numbness, pain in limbs, rashes, retinitis, schizophrenia, shyness, speech disorders, suicidal tendencies, tingling, tremors (eyelids, lips, tongue, fingers, extremities), vision loss, weakness
Goals of the Model Design
• Simultaneous detection of mercuric and arsenic contamination
• Accumulating and cleaning mercury and arsenic.
Ars Operon
Mercury Operon
Cloning modules
Transporting Module • GlpF, a aquaglycerol porin from E. coli• Citrate coupled transporters, CitM from
Bacillus subtilis• Promoter + MerR + MerP + MerT
Cloning modules
Detecting Module• Arsenic promoter from E. coli, pArsR + RBS +
GFP• Mercury-Inducible Promoter from E. coli,
pMerR + RBS + RFP
Cloning modules
Sequestering Module• Metallothioneins• pArsR + RBS + GFP + pArsR + RBS + fMT
(arsenic form)• pMerR + RBS + RFP + pMerR + RBS + MerR +
MerP + MerT + fMT (mercuric form)
Cloning modules
Buoyancy Module• gas vesicle protein gene cluster from Bacillus
megaterium, GVP • pArsR + RBS + GFP + pArsR + RBS + fMT
(arsenic form) + RBS + GVP• pMerR + RBS + RFP + pMerR + RBS + MerR +
MerP + MerT + fMT (mercuric form) + RBS + GVP
Topological Sort
Let’s Make a Deal
Sudoku
Acknowledgement
• All organizers• All participants
Thank you!