a ntibody -b ased t herapeutics for t reating a lzheimer ’ s and p arkinson ’ s d iseases taylor...
DESCRIPTION
R ESEARCH O BJECTIVES Process of protein misfolding and accumulation causes disease β-amyloid and α-synuclein are two biomarkers (proteins) linked to AD and PD Oligomeric forms of β-amyloid and α-synuclein are the key toxic species 3 Identify antibodies to detect β-amyloid and α-synuclein Can be used for therapeutics and diagnosticsTRANSCRIPT
ANTIBODY-BASED THERAPEUTICS
FOR TREATING ALZHEIMER’S AND PARKINSON’S DISEASES
Taylor Brownlee
Dr. Michael SierksChemical Engineering
2012 Arizona/NASA Space Grant SymposiumUniversity of Arizona
April 21, 2012
WHAT CAUSES ALZHEIMER’S AND PARKINSON’S DISEASES? Alzheimer’s disease (AD) and Parkinson’s disease (PD)
are devastating neurodegenerative diseases
Plaques and tangles (AD) or Lewy bodies (PD) Accumulate in different areas of the brain Block nerve cell
communication and cause cell death
Presence of plaques and tangles or Lewybodies is not a definitivediagnosis for AD or PD
2
http://www.ahaf.org/alzheimers/
RESEARCH OBJECTIVES Process of protein misfolding and accumulation
causes disease
β-amyloid and α-synuclein are two biomarkers (proteins) linked to AD and PD
Oligomeric forms of β-amyloid and α-synuclein are the key toxic species
3
Identify antibodies to detectβ-amyloid and α-synuclein
Can be used for therapeutics and diagnostics
SANDWICH ELISA 4
5
1E-05 1E-04 1E-03 1E-020.26
0.27
0.28
0.29
0.30
Phage (mg/mL)1E-061E-07
PD Biomarker (mg/mL)
PD B
iom
arke
r Sig
nal
RESULTS FOR PD ANTIBODY
CONCLUSIONS Process of protein misfolding and accumulation
causes Alzheimer’s and Parkinson’s diseases
Antibodies detect toxic biomarker species that cause AD and PD
Sandwich ELISA detects the biomarker concentration
1E-07 M13 bacteriophage concentration yielded the largest biomarker signal
Further study must be done to perfect ELISA protocol
6
THANK YOU!
QUESTIONS?