a network of regulatory interactions governed by hfq
DESCRIPTION
A network of regulatory interactions governed by Hfq. Hfq has been shown to bind each of the ncRNA (circled) and mRNAs (outer ring) facilitating the post-transcriptional gene regulation that responds to the indicated environmental stress. From the Feig Laboratory - PowerPoint PPT PresentationTRANSCRIPT
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A network of regulatory interactions governed by Hfq.Hfq has been shown to bind each of the ncRNA (circled) and mRNAs (outer ring) facilitatingthe post-transcriptional gene regulation that responds to the indicated environmental stress.
From the Feig LaboratoryWayne State University WEB Page
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From the Feig LaboratoryWayne State University WEB Page
Secondary structures of DsrA (top) and rpoS mRNA 5'-UTR(bottom) The color coding of DsrA indicates the accepted domain structure. For rpoS, the open reading fram is shown in green and the region complementary to DsrA (bases 11 - 32 in DsrA) is shown in lavender.
Generic model through which regulatory ncRNAs can relieve translational repression of a complementary mRNA through strand displacement. Equivalent models can be used to explain translational repression as well.
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Principle of pyrosequencing
http://www.s3.kth.se/mst/research/projects/beadbasedmicrofluidic/index.shtml
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How to detect the gene expression? (1) Northern Blot analysis
(2) Primer extension analysis (3) S1 nuclease mapping
(4) Nuclease protection assay
(5) RT-PCR
(6) Large-scale cDNA sequencing
(7) Microarray analysis
(8) 5’-, 3’- RACE (Rapid Amplification of cDNA En
ds)(9) Reporter gene assay
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Northern Blot Hybridization
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Figure 4.12. Southern hybridization. (A) Transfer of DNA from the gel to the membrane. (B) The membrane is probed with a radioactively labeled DNA molecule. On the resulting autoradiograph, one hybridizing band is seen in lane 2, and two in lane 3.
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Primer Extension Analysis
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S1 Nuclease Mapping Analysis
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Gene Gene product Assay
lacZ β-galactosidase Histochemical test
uidA β-glucuronidase Histochemical test
lux Luciferase Bioluminescence
GFP Green fluorescent protein
Fluorescence
Reporter Gene Assay
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Luciferase gene
Luciferase Activity (Promega)
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PITX2-LACZ Staining of mouse embryo day 11
PITX2: bicoid-related transcription factor
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