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A. JOHNS HOPKINS -BIDOMBERG ".f' SCHOOLtfPUBLlC HEALTIi

Application Review Sheet This form is provided for your convenience, and does not need to be returned to Admissions Services. Please remember to update the

r t d' th A I ~ If S t d d fth d rt t' d .. I tt t Ad .. S . Th k appllcan recoli In e IPPIV ourse iys em, an sen a copvo e epa men s eClslon e er a mIssIons ervlces. an . you.

Applicant Name: Chishimba Sandra Mushili Country of Citizenship: Zambia Degree I Program: PhD: Molecular Microbiology al Molecular Microbiology and Immul Current Profession: laboratory Scientist Date Application Forwarded to DepartmenUProgram: Research Interests: Year / Term: September 2012 Immune responses to infections

I. THIS APPLICANT IS ADMITTED: DYES DNO D Recommend to:

for DEGREE PROGRAM: DIVISION or TRACK:

to begin: D Summer Term D 1st Term D 2nd Term D3'd Term D4th Term D Winter Institute D Summer Institute

Comments:

GRE (official only) Test Date VScore / %ile QScore / %ile AScore / %ile Is Official 12 2011 147 /36 146 / 36 3.5 / 29 Y

/ / / / / /

GMAT (official only) Test Date VS co re QScore Total Writing Is Official

TOEFL (official only) Test Date Test TYl2e Listening Writing Reading Sl2eaking / Essay Total Is Official

MeAT (official only) Test Date Verbal Physical Science Writing Saml2le Biological Science Total Is Official

Letters of Recommendation Recommender Name Relationshil2 Received Date

Peter Agre Employer MRT/JHMRI

Tom Cupedo Former supervisor

Sungano Mharakurwa Employer

Previous Schools School Name Degree Major Degree Date GPA Erasmus Univ MS IMMUNOLOGY 08/2012

Work History Eml2loyer Location Working From Working To Macha Research Trust! JHMRI Choma ZAMBIA 01/2007

Bank of Zambia Ndola ZAMBIA 11/2002 05/2003

KBS Insurance Brookers Ndola ZAMBIA 05/1999 08/2000

JOHNS HOPKINS BLOOMBERG SCHOOL o/'PUBLlC HEALlli

Admissions Services Student Affairs 615 N. Wolfe Street, Suite E1002 Baltimore, Maryland 21205-2179

ApPLICATION FOR ADMISSION

PERSONAL INFORMATION

1. Chishimba Sandra Mushili Ms.

Last name (surname or family name) First name l'vliddle name

Zambia

Salutation SufEL" Former Name

2. Social Security Number (U.S. applicants only)

3. 05/02/1981 Date 0 f birth

4. Gender:

Email Address

D Male rn' Female

Lusaka City of birth

5. [email protected] Primary

Country of Citizenship

Secondary

Zambia Country of birth

Current Contact Information-valid until _________ _

6. 's-Gravendijkwal 155A

Address line one

Address line two

7. Rotterdam 3021 EM City State Zip or postal code

English

Native Language

Netherlands Countty

8. AND AND +31 644738970 Residence Phone (lntl Ctry Code, Phone #) I Business Phone (lntl Ctry Code, Phone #) I Cell Phone (lntl Ctry Code, Phone #)

Permanent Contact Information-valid until

's-Gravendijkwal 155A 9.

Address line one

Address line two

Rotterdam 10.

City State

11.

Zip or postal code

Permanent Residence Phone (Inti Ctry Code, Phone #)

3021 EM

Race and Ethnicity-U.S. Citizens and Permanent Residents Only

12a. Are you Hispanic or Latino? DYes DNo

12b. Race?

D American Indian or Alaska Native

D Asian

D Native Hawaiian or other Pacific Islander

D Black or African American

DWhite

Netherlands

Country

Chlshimba , Sandra Molecular Microbiology and Immunology PhD (Doctor of Philosophy) • Molecular Microbiology and Immul

13. Which of the following sources stimulated your interest in the Johns Hopkins Bloomberg School of Public Health? (Check all that apply.)

D Advertisement ________________ _

D Alumni ofJHSPH ____________ _

[i( Employer Macha Research Trust! JHMRI

D Graduate Fair ________________ _

DJHSPH Student _____________ _

D Web Chat ________________ _

PROPOSED ACADEMIC PROGRAM

14. September 2012

Academic Year and Term

15. Molecular Microbiology and Immunology Department

16. Immune responses to infections Research Interest (if any)

D Adviser ________________ _

D Colleague ______________ _

D Faculty ______________ _

D JI-ISPH Campus Visit __________ _

D Website _________________ _

D Other _______________ _

PhD (Doctor of Philosophy)

Degree Program

Molecular Microbiology and Immunology Track/Division (if any)

NOTE! GENERAL PREVENTIVE MEDICINE APPLICANTS FOR THE PRACTICUM YEAR MUST HAVE EARNED THEIR MPH AT JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH.

ACADEMIC HISTORY

17. Please list all colleges, universities, and graduate or professional schools attended in reverse chronological order (most recent fIrst).

College or City, State Country Indicate if attended Attended From Attended To Major(s)/Minor(s) Degree GPA/GPA University as Undergraduate (mm/yyyy) (mm/yyyy) Received Scale

or Graduate or Expected Date

Erasmus Univ Rotterdam NETHERLANDS Graduate 08/2010 08/2012

IMMUNOLOGY MS

Hopkins History

18. Have you applied to the Johns Hopkins Bloomberg School of Public Health previously? ri No D Yes: Year of Application ______ _

19. Have you attended, or are you currently attending, a Hopkins institution? I3l'N 0 D Yes: Year Degree Received/Expected _______ _

Division __________________ _

20. Are you a current Hopkins faculty member? ri NoD Yes: Division _________________ _

21. Are you a current Hopkins employee? I3l'N 0 D Yes: Division ____________________ _

Additional Information

22. I-IJve you ever been discontinued or di,mh,ed [rom any school or college for reason of scholarship, discipline, or violation of academic ethics? rn No DYes If yes, please provide an explanation:

2

Chishimba , Sandra Molecular Microbiology and Immunology PhD (Doctor of Philosophy) , Molecular Microbiology and ImmUi

EMPLOYMENT HISTORY

23. Please provide a history of your professional and/ or research-related experience in reverse chronological order (most recent first).

Position Title Organization Name and Location Fonn (mm/yyyy) To (mm/yyyy)

Macha Research Trust/ JHMRI I Choma ZAMBIA

0112007

Clerk Bank of Zambia 1112002 0512003 Ndola ZAMBIA

Receptionist/Clerk KBS Insurance Brookers Ndola ZAMBIA

0511999 0812000

24. Please provide your current professional category. Select from the list provided. Laboratory Scientist

25. A copy of your resume or curriculum vita is required. I-lave you enclosed it with your application? [fYes D No

Additionaiinformation

26. I-lave you ever been convicted of a criminal offense, or are there criminal charges pending against you? DYes [!( No

If yes, please provide an explanation:

27. Have you ever been terminated from employment or professional practice for any reason, or have there ever been any charges of ethical/professional misconduct filed against you? D Yes Ii No If yes, please provide an explanation:

TEST SCORES

28. Please enter the date on which you have taken, or expect to tal,e, the GRE, Gl'v1AT, MCAT, TOEFL, IELTS or other test, and enter the score and percentile, if known.

Note! The ETS test code for the Johns Hopkins Bloomberg School of Public Health is 5352.

GRE-Test Date (mm/yyyy)

Verbal Score %ile Quant. Score %ile Analytical Score "Ioile Analytical Writing %ile

Verbal Score %ile Quant. Score %ile Analytical Score %ile Analytical Writing %ile

GMAT-Test Date (mm/yyyy)

Quant. Score %ile Verbal Score %ile AWA Score %ile Total Score %ile

MeAT-Test Date (mm/yyyy)

Ploys. Score%ile Bio. Sci. Score %ile Verbal Reas. Score %ile· Writing Sample %ile

3

Chlshimba , Sandra Molecular Microbiology and Immunology PhD (Doctor of Philosophy) , Molecular Microbiology and ImmUl

TOEFL-

Test Date (mm/yyyy) ________ _

Listening Structure/Writing Reading Test of Written English (TWE) Total Score

Computer-Based Scores D / Internet-Based Scores D /Paper -Based Scores D

Test Date (mm/yyyy) ________ _

Listening Structure/Writing Reading Test of Written English (I'WE) Total Score

Computer-Based Scores D / Internet-Based Scores D /Paper -Based Scores D

IELTS-

Test Month ______ Test year _____ _

IELTS Registration Number ______________ Band Score __________________ _

Listening Speaking Reading Writing

Test Month _____ _ Test year ______ _

IELTS Registration Number ______________ Band Score __________________ _

Listening Speaking Reading Writing

Other-

Test Date (mm/yyyy) _________ _

Test Name Total Score

OTHER INFORMATION

Medical Graduates

Note! This section is to be completed by medical graduates only.

13. Please provide YOllr internship and residency experience in reverse chronological order (most recent first). Format all dates as mm/yyyy.

Chishlmba , Sandra Molecular Microbiology and Immunology PhD (Doctor of Philosophy) • Molecular Microbiology and ImmUi

Name of Institution

a.

b.

c.

Specialty Begin Date End Date

Board Cert. Country Date

Board Eligible

30. If you hold a medical license in the United States or Canada, please indicate state or province and provide your medical examination information.

State or Province _____________________ _

US~EExaminationScores: ____________________________________ __

Score I Score II Score III Score IV

Other Medical Examination: __________________________________ _

Name Score

31. If you are a graduate of a medical institution outside the United States or Canada, do you possess ECFMG certification?

Date Issued (mm/yyyy): ______ _ Certificate Number: _____________ _

International Students

Note! This section is to be completed by all non-U.S. citizens only.

32. Are you a permanent resident of the United States? DYes [iNo Alien Registration Number: _____________________ _

33. If you are not a U.S. citizen, do you currently hold a U.S. visa? [(Yes DNo

If yes, what type of visa do you currently hold?

D F-l Student: Visa Number ________________________________________ _

D J-1 Exchange: Visitor/Student Visa Number ____________________________________ _

[i'Other B1IB2 Visa Number C2560915 ---------------------

FINANCIAL ASSISTANCE

34. Do you intend to apply for Federal Student Aid (student loans and/or workstudy)? DYes DNo

35. If you are being funded by, or intend to be funded by, an external agency, please indicate below.

D Fogarty D Fulbright D Humphrey D Other

PERSONAL STATEMENT

36. This part of the application is vital to the admissions review process. Please read the application instructions to make sure you provide the most useful statement possible. Since this statement is your first opportunity to demonstrate your own writing skills to our faculty, make sure you submit a concise, well-constructed essay. Please limit your statement to two pages or less and provide your name on the top of each page.

REFERENCES

37. Please provide the names, positions, addresses, telephone numbers, and email addresses of the three persons who will be providing references for you.

Peter Agre University Professor and Direct a. ____________________________________________ ,-________ _

Online Provider li'Yes DNo

[email protected] Name

615 North Wolfe Street

Address

b. Tom Name

Cupedo

Position

443-287-8745

Telephone Email

Assistant Professor

Position Online Provider [(Yes DNo

5

Chisrnimba , Sandra Molecular Microbiology and Immunology PhD (Doctor of Philosophy) , Molecular Microbiology and ImmUi

+31.(0)10.7044082 [email protected]

Address Telephone Email

Mharakurwa Scientific Director Sungano c. ______________________________________________________________________________ ~----~~--------

Name Position Online Provider !!l' Yes DNo

Choma-Namwala Rd [email protected]

Address Telephone Email

REFERENCES

38. We will not share information regarding your application with anyone else without your permission. Please provide name, address, and telephone number of persons or agencies authorized by you to obtain information regarding your application. Include your relationship to this individual (i.e., parent, spouse, friend).

a. Name: Phone: Relationship:

b. Name: Phone: Relationship:

Name: Phone: Relationship: c.

39. Please read the following statement and indicate by your signature below that you agree to the terms stated. Unsigned applications will be returned.

I affinn that the information on this application form, and any additional material that I submit related to the admissions process, is complete, accurate, and true to the best of my knowledge. I agree to submit any other materials that are required for the admissions process. I understand that furnishing false or incomplete information on any part of this application for admission or any related materials may be cause for denial of admission, cancellation of registration, or revocation of a degree. I understand that my application fee is non-refundable and that any materials submitted related to my application become the property of the Johns Hopkins Bloomberg School of Public Health and cannot be returned or

forwarded to a third party. I understand that the decision made on my application is fInal and not subject to appeal. Finally, I agree that if admitted as a student, I will honor the academic ethics code and the student conduct code of the Johns Hopkins Bloomberg School of Public Health.

S· tu Sandra Chis hi mba 19na re: ________________________________________________________________ ___ Date: 1/4/20121:09:00 PM

8.2009

6

JOHNS HOPKINdSl BIDOMBER( ; SCHOOL/PUBLIC HEArM;

MPH ApPLICANT SUPPLEMENTAL FORM

Applicant's name: _C_h_i_s_h_im_b_a ________ S_a_n_d_ra _______ M_u_s_h_il_i ___________________ _ Last name First name Nliddle name Former name(s) if applicable

Social Security No. (U.S. applicants only): _________________ _

What is your primary area of interest (for the purposes of faculty adviser assignment)?

SECTION ONE: PROFESSIONAL HEALTH-RELATED EXPERIENCE

Are you a dentist, veterinarian, medical student or physician? DYes D No

If you indicated YES, please sldp to the final question on this form because your training in medical school fulfills your course and work experience requirements.

Applicants to the MPH program must have at least two years of post-baccalaureate, full-time health-related work experience or a doctoral degree in a field underlying public health. This health-related experience requirement is waived for applicants to the MPH/JD, NIPH/MSN, and MPH/MSW dual degree programs.

D I have 2 years of health-related experience. (YOH will be asked to provide details in Section FOHr below.)

D I have a doctoral degree in ______________________________________________ _

SECTION Two: REQUIRED COLLEGE COURSES

You r,rmst submit an official transcript or mark sheet for every college attended.

At least one college course in mathematics is required (e.g., algebra, calculus, statistics). Please list college courses you have completed in mathematics.

Course title College Date taken Grade


At least two college courses related to human biology are required (e.g., biology, microbiology, anatomy, and physiology). Please list college courses you have completed in areas related to human biology.




If you anticipate completing any of the course requirements after submitting your application, please fill out the following section. Please note that coursework must be completed prior to your anticipated matriculation date, and that the Admissions Office must have an official copy of the additional transcript prior to your matriculation into the program.

Course title College Expected d'1te of completion

Course title College Expected date of completion

SECTION THREE: TEST SCORE ACKNOWLEDGMENT

Do you have an advanced degree beyond the baccalaureate degree (such as a MS, MA, MD, DDS, MBBS, PhD, etc.)? DYes D No

If you indicated NO, you must submit a standardized test score as part of the application process.

If you indicated YES, you may elect to have your application reviewed by the MPH Admissions Committee without a standardized test score such as the GRE, MCAT, GMA T, or LSAT. However, you may be putting yourself at a disadvantage, particularly if your admission materials do not demonstrate significant quantitative and analytical skills.

Applicants who request to have their application reviewed without a standardized test score may significantly reduce their chances of being awarded a scholarship. Applicants who have an advanced degree and wish to have their application reviewed without a standardized test score should check the "YES" box below.

I have read the statement above and I would like to have my application reviewed by the MPH Admissions Committee without submitting a standardized

test score. DYes D No

SECTION Four: PROFESSIONAL HEALTH-RELATED EXPERIENCE - DETAILS

Applicants to the MPH program must have at least two years of post- baccalaureate, full-time, health-related work experience. Please provide details of your health-related experience. Be sure to include a current resume or curriculum vita with your application materials.

NOTE! As stated in Section One above, tbe health-related experience requirement is waived for most applicants who possess a doctoral degree or are applying for the .MPH/JD, MPH/MSN, or MPH/MSW joint degree programs.

Position tide Period of employment Hours per week

Role and Scope of Responsibility

Position title Period of employment Hours per week






· Resume Curriculum Vitae

Curriculum Vitae

Personal data

Name: Sandra Mushili Chishimba

Date of birth: May 2nd, 1981

Place of Birth: Lusaka

Sex: Female

Nationality: Zambian

Marital Status: Single

Address: 's Gravendijkwal 155A

3021 EM,

Rotterdam,

Netherlands

E-mail: [email protected]

[email protected]

Skype: [email protected]

Tel: +31 (0)644 738970

CURRENT APPOINTMENTS

Sandra Chishimba 3499608

2007 - Present: Laboratory Scientist Macha Research Trust/Johns Hopkins Malaria Research Institute

2009 - Present: Reviewer, Institutional Review Board/Ethics Committee (IRB/EC)Malaria institute at Macha/Malaria Research Trust (MIAM/MRT)

· ResLlme Curriculum Vitae

EDUCATION


2010 - Present: Master of Science Infection and Immunity Candidate, Erasmus Medical Center Rotterdam, Netherlands

2000 ·2006: Bachelor of Science, University of Zambia

1994 ·1998: Secondary School, Fatima Girls High School

RESEARCH WORKING EXPERIENCE

Sept. 2011 - Present: The role of vaccination as a diagnostic tool for identification of patients with a primary antibody deficiency (PAD). Principal Investigator: Mirjam van der Burg, Erasmus Medical Center Rotterdam, Netherlands.

Feb. 2011- July 2011: In situ localization of human RORC+ innate lymphoid cells. Department of Hematology. Principal investigator: Tom Cupedo, PhD, Erasmus Medical Center Rotterdam, Netherlands.

Dec. 2010: Laboratory rotation MSc student, Department of Immunology, Antibody deficiencies: searching for the unknown. Mapping deletions in the XIAP gene. Principal Investigator: Mirjam van der Burg, PhD, Erasmus Medical Center Rotterdam, Netherlands.

Nov. 2010: Laboratory rotation MSc student, Department of Hematology, Development of lymphoid organs. Learning lab techniques on RT-PCR, Tissue staining, and FACS analyses. Principal Investigator: Tom Cupedo, PhD, Erasmus Medical Center Rotterdam, Netherlands.

Oct. 2010: Laboratory rotation MSc student, Department of Immunology, Ig repertoire selection for protective immunity. Learning lab techniques. Principal Investigator: Menno C van Zelm, PhD, Erasmus Medical Center Rotterdam, Netherlands.

2010: Research on using dried blood spots to measure humoral immunity to measles and rubella by ELISA method. Principal Investigator: Dr William J Moss, Johns Hopkins University School of Public Health

2010: Research on the use of oral fluid to monitor changes in humoral immunity to Plasmodium fa/ciparum in a region of declining malaria transmission. Principal Investigator: Dr William J Moss, Johns Hopkins University School of Public Health

2008 - 2010: Monitoring changes in Antibody (lgG) levels to Plasmodium falciparum in a region of declining malaria transmission (Macha). Principal Investigator: Dr William J Moss, Johns Hopkins University School of Public Health

· Resume Curriculum Vitae Sandra Chishimba 3499608

2009: Research on detection of latent ultra low Plasmodium falciparum infections using tertiary nested PCR and short amplicon primers (Macha). Principal Investigator: Dr Sungano Mharakurwa, Malaria Institute at Macha

2007: Research on rare drug resistance alleles in Mosquito of Plasmodium fa/ciparum. Principal Investigator: Dr Sungano Mharakurwa, Malaria Institute at Macha

2007: Research on Urinary Schistosomiasis in school children rural Zambia (Chitongo). Principal Investigator: Dr Clive J Shiff, Johns Hopkins University School of Public Health

2008: Research on Rapid diagnostic test (ROT) kit and their effectiveness with malaria diagnosis using human saliva. Principal Investigator: Dr Sungano Mharakurwa, Malaria Institute at Macha

2007: Research on escalating Plasmodium falciparum antifolate drug resistance mutation in rural Zambia. Principal Investigator: Dr Sungano Mharakurwa, Malaria Institute at Macha

2007: Research on a simple DNA extraction protocol on wild Anopheles spp. Principal Investigator: Dr Sungano Mharakurwa, Malaria Institute at Macha

2007 ·Present: Research on prevalence of Plasmodium fa/ciparum parasitemia and gametocytemia in a region of unstable malaria transmission. Principal Investigator: Dr William J Moss, Johns Hopkins University School of Public Health

2007: Research on using human saliva and urine for the detection of Plasmodium fa/ciparum. Principal Investigator: Dr Sungano Mharakurwa, Malaria Institute at Macha

PRIOR WORKING EXPERIENCE

July 2005: Practical period as an undergraduate student, Pathology department, mainly Microbiology laboratory and Biochemistry laboratory carrying out routine laboratory work, Ndola Central Hospital (NCH).

Jan-Feb 2005: Malaria research field team member and lab student trainee undergraduate student work collecting data and samples from communities and Laboratory work performing extractions of DNA of the parasite, Malaria Institute at Macha (MIAM) and Macha Mission Hospital Laboratory carrying out routine lab work.

2005: Participated in Course project on Bilharzia control in Ngombe compound, under the University of Zambia. Principal Investigator: Dr. Shehata.


2002 - 2003: Reconciling debit and credit books, banking reconciliation, Banking department, Bank of Zambia (BOZ).

1999- 2000: Clerical work and Receptionist, KBS Insurance Brokers.

PUBLICATIONS

Mharakurwa S, Kumwenda T, Mkulama MA, Musapa M, Chishimba S, Shiff CJ, Sullivan OJ, Thuma PE, Liu K, Agre P. Malaria antifolate resistance with contrasting Plasmodium falciparum dihydrofolate reductase (OHFR) polymorphisms in humans and Anopheles mosquitoes. Proc Natl Acad Sci USA. 2011.

Alexis P Chidi, Sandra Chishimba, Tamaki Kobayashi, Harry Hamapumbu, Sungano Mharakurwa, Philip E Thuma, William J Moss: Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum: an observational study in southern Zambia. Malar Journal 2011, 10:162

Mkulama APM, Chishimba S, Sikalima J, Rouse P, Thuma EP, Mharakurwa S: Escalating Plasmodium falciparum antifolate drug resistance mutation in Macha, rural Zambia. Malar J. 2008; 7: 87.

Chishimba S, Kamanga A, Sikalima J, Clennon J, Mharakurwa S, Shiff JC: Urinary schistosomiasis scourge among rural school children in Chitongo area, Southern Zambia. Medical Journal of Zambia 2007; 34:141-145.

ABSTRACTS

Sandra Chishimba, Tamaki Kobayashi, Musapa Mulenga, Masiliso Phiri, Sungano Mharakurwa, Philip Thuma, William J. Moss: The Impact of insecticide-treated nets on acquired humoral immunity to Plasmodium falciparum. The American Journal of Tropical Medicine and Hygiene 2010; 83:223

Alexis Chidi, Sandra Chishimba, Tamaki Kobayashi, Sungano Mharakurwa, William J. Moss: Use of an oral fluid assay to monitor changes in humoral immunity to Plasmodium falciparum in a region of declining malaria transmission in Southern Zambia. The American Journal of Tropical Medicine and Hygiene 2010; www.astmh.org

Tamaki Kobayashi, Sandra Chishimba, Sungano Mharakurwa, Philip Thuma, William J. Moss: Increasing antibodies to Plasmodium falciparum can be used to identify spatial clusters of malaria transmission in a region of declining malaria Southern Zambia. The American Journal of Tropical Medicine and Hygiene 2010; www.astmh.org

· ResLlme Curriculum Vitae Sandra Chishimba 3499608

Masiliso Phiri, Tamaki Kobayashi, Sandra Chishimba, Gillian Stresman, Sungano Mharakurwa, Philip Thuma, William J. Moss: The Predictive value of rapid diagnostic tests for Gametocytemia identified by RT -PCR. The American Journal of Tropical Medicine and Hygiene 2010; 83:84

Sungano Mharakurwa, Taida Kumwenda, Mtawa Mukulama, Mulenga Musapa, Sandra Chishimba, Clive J. Shiff, David Sullivan, Philip Thuma: Underrating mosquitoes again:Surveillance evasion by Plasmodium falciparum antifolate drug resistance mutants. The American Journal of Tropical Medicine and Hygiene 2010; 83:205

Kobayashi T, Chishimba 5, Thuma P, Mharakurwa S, Kumar N, Moss JW: Using Dried Blood Spots to Monitor Changes in Antibody Levels to Plasmodium falciparumin a Region of Declining Malaria Transmission. The American Journal of Tropical medicine and Hygiene 2009; http://www.astmh.org.

Kobayashi T, Phiri M, Chondoka J, Chishimba 5, Siame M, Sikalima J, Thuma P, Mharakurwa S, Mlambo G, Moss W: Comparison of Dried Blood Sample-Based RTPCR against Microscopy for the Detection of P. falciparum Gametocytemia in Southern Zambia. Presented at the Multilateral Initiative on Malaria (MIM) and the Zambia National Health Research conference 2009.

Siame M, Chondoka J, Chishimba 5, Musapa M, Thuma P, Mharakurwa S: Detection of latent ultra low Plasmodium falciparum infections by tertiary nested PCR and short amplicon primers. Presented at the Multilateral Initiative on Malaria (MIM) Conference and the Zambia National Health Research Conference 2009.

Chishimba 5, Kamanga A, Sikalima J, Clennon J, Mharakurwa S, Shiff JC: Urinary schistosomiasis scourge among rural school children in Chitongo area, Southern Zambia. The American Journal of Tropical Medicine and Hygiene 2008; 79:89

Mharakurwa S, Mkulama AM, Chishimba 5, Sikalima J, Shiff JC, Sullivan JD, Thuma EP: Immunochromatographic Detection of P. falciparum infection using Human saliva and urine samples. The American Journal of Tropical medicine and Hygiene 2008; 79:116.

Mharakurwa S, Kumwenda T, Mkulama M, Musapa M, Chishimba 5, Sikalima J, Norris D, Shiff JC, Thuma EP: Rare drug resistance alleles thrive in Mosquito phase Plasmodium falciparum. The American Journal of Tropical Medicine and Hygiene 2007; 77:77.

Musapa M, Mkulama M, Chishimba 5, Rouse P, Norris D, Thuma EP, Mharakurwa S: Comparisons of a simple Chelex procedure against standard salt protocol for DNA extraction from wild Anopheles spp. The American Journal of Tropical Medicine and Hygiene 2007, pp 50-99.


PROFESSIONAL SOCIETIES

2008- Present: Member of the American Society for Microbiologists (ASM)

OTHER EDUCATIONAL EXPERIENCES


2011: 61 st Lindau Nobel Laureate Alumni, Meeting dedicated to Physiology/Medicine, Lindau, Germany.

2011: The 4th Symposium & master classes on Mucosal Immunology "Adaptive immune response in the mucosa: B cells and beyond". Erasmus Medical Center Rotterdam, Netherlands

2011: Photoshop and Illustrator CSS Workshop for PhD-students and other researchers, Erasmus Medical Center Rotterdam, Netherlands

2011: The Basic Introduction Course on SPSS, Erasmus Medical Center Rotterdam, Netherlands

2010: Systems Applied Bioinformatics, Sequences and Variations, Erasmus Medical Center Rotterdam, Netherlands

2010: 2Sth Erasmus Liver Day, Erasmus Medical Center Rotterdam, Netherlands

2010: Basic Course in Human Genetics: Genetics for Dummies, Erasmus Medical Center Rotterdam, Netherlands

2010: Biomedical Research Techniques IX, Erasmus Medical Center Rotterdam, Netherlands

2010: Writers workshop Certificate Medical Journal of Zambia

2009: Certificate in Good Clinical Practice (GCP), Malaria Research Trust

AWARDS

2011: Infection and Immunity research grant

CONFERENCES ATTENDED AND PRESENTATIONS

2011: 61 st Lindau Nobel Laureate Meeting dedicated to Physiology/ Medicine, Lindau, Germany

, Resume Curriculum Vitae

1. Global Health discussion Panelist


2. Microsoft presentations-61 st Lindau Nobel Laureate Meeting: Monitoring Malaria Serology in a Malaria declining region Macha, Southern Zambia

Presenter

2010: American Society for Tropical Medicine and Hygiene, USA 59th Annual Meeting Atlanta, Georgia

1. The impact of Insecticide treated nets on acquired humoral immunity to Plasmodium fa/ciparum

Position: Co-chair and Presenter

2010: Ministry of Health, National Health Advisory Committee Technical Working Group Fifth Workshop at NMCC. Stakeholders' consultative meeting TWG 5. Formation of National Health Research Council of Zambia (NHRCZ).

Position: Chair Group 3 Topic: Legal framework and strategy for getting document into Law.

2009: National Health Research Conference and Symposium on Non- Communicable Diseases Lusaka, Zambia

1. Chishimba S, Kamanga A, Sikalima J, Clennon J, Mharakurwa S, Shiff JC: Urinary schistosomiasis scourge among rural school children in Chitongo area, Southern Zambia. Medical Journal of Zambia 2007; 34:141-145.

2. Kobayashi T, Chishimba S, Thuma P, Mharakurwa S, Kumar N, Moss JW: Using Dried Blood Spots to Monitor Changes in Antibody Levels to Plasmodium fa/ciparum in a Region of Declining Malaria Transmission.

Presenter and participant

2009: Malaria Training and Research Capacity Building in Southern Africa (MTCBSA) International Workshop on Biology, Epidemiology and Management of Malaria. Malaria Institute at Macha, Choma, Zambia.

Participant

2008: American Society for Tropical Medicine and Hygiene, New Orleans, Louisiana, USA 5ih Annual meeting


1. Chishimba S, Kamanga A, Sikalima J, Clennon J, Mharakurwa S, Shiff JC: Urinary schistosomiasis scourge among rural school children in Chitongo area, Southern Zambia. Medical Journal of Zambia 2007; 34:141-145.

Presenter and Participant

REFERENCES

Philip Thuma, M.D

Senior Scientific Advisor

Macha Research Trust / Malaria Institute at Macha

P. O. Box 630166

Choma, Zambia

Main Office Phone/Fax: +260213 261 022

Mobile: +260977721 599

Email: phi [email protected]

Peter Agre, M.D.

University Professor and Director

Johns Hopkins Malaria Research Institute

Bloomberg School of Public Health

615 North Wolfe Street, Room E5143

Baltimore, Maryland 21205-2179

Phone: 443-287-8745

Fax: 410-955-0105



Sungano Mharakurwa, PhD

Macha Research Trust! Johns Hopkins Malaria Research Institute

Scientific Director

P.O Box 630166

Choma, Zambia

Phone: +26 097931 2796

Fax: +260213261022


Tom Cupedo, PhD

Assistant Professor

Erasmus University Medical Center

Department of Hematology, Room Ee1330f

PO Box 2040

3000 CA Rotterdam

The Netherlands

e-mail: [email protected]

phone +31.(0)10.7044082 fax +31.(0)10.7044745

http://tiny.cc/cupedolab

November 12, 2011


Statement of Experience Goal Sandra Chishimba


Having suffered from malaria occasionally as a child and during adulthood, and seeing the many tropical diseases that were ravaging the communities in my country Zambia and Africa as a whole I realized that I wanted to make a contribution to medicine either by helping understand the diseases that affect us and by treating people as a medical doctor or participate in vaccine development. I came to the conclusion that research and ongoing education were the best tools that society needs to protect itself from diseases. I therefore started participating in science fairs by making project presentations on different science topics during my high school.

My interest for microbiology and molecular biology began during my undergraduate studies and was the impetus for my desire to do infectious disease research. In my third year at the University of Zambia (2005), I pursued an internship at the Malaria Institute at Macha (MIAM)/Johns Hopkins Malaria Research Institute also known as Macha Research Trust. During this internship I spent a month mastering the techniques of DNA extraction and Polymerase Chain Reaction (PCR) from Plasmodium Jalciparum. In addition, I did field work where I participated in collecting blood samples from people in the communities around Macha.

In 2007, I joined the MIAM research staff as a Laboratory Scientist in the Malaria Molecular biology laboratory with an emphasis on immunity against the malaria parasite in human blood. The aim of the research was to determine how levels of antibodies against P. Jalciparum change due to intensified malaria control measures and decreasing transmission intensity. The second objective was to develop a simple, field-friendly, method to measure immunity to P. Jalciparum and to indentify populations at risk for disease resurgence. I performed Enzyme immunoassay to measure IgG levels using P. Jalciparum NF54 strain schizont cell lysate on dried blood spots (DBS). In this work, I interacted routinely with principle investigators at Johns Hopkins Malaria Research Institute Dr William Moss MD (project director) and Dr Tamaki Kobayashi PhD (coinvestigator), and I also participated in weekly lab meetings. The preliminary results from the study were as follows: The Optical Density (OD) values of the IgG antibodies in children younger than 10, increased with age. These results indicated that immunity in young children takes longer to develop and is dependent on frequent exposure. A year after distribution of insecticide treated bednets (ITN s) there was no association between bed net use and seropositivity in children younger than 10, probably due to lack of frequent exposure. In individuals older than 20 years a significant association was observed between seropositivity and bed net use. This could indicate that decrease in exposure to P. Jalciparum should lead to decreased levels of immunity. There was spatial clustering of individuals with increasing IgG antibodies against P. Jalciparum a potential indicator for focal malaria transmission in our study area.

The third objective of the malaria project was to develop a non-invasive way of estimating population immunity to P. Jalciparum using oral fluid (OF) as a surrogate to blood samples. Our data showed that oral fluid is a valid measure for estimating population immunity against P. Jalciparum antigen. The OF assay identified all DBS-confirmed positive and negative samples, resulting in 100% sensitivity and specificity. The preliminary data from this work has been presented at the American Society for Tropical Medicine and Hygiene (December, 2008 and November 2011) and the Zambia National Health Research Conference (September 2009). I presented the work at the 61st Lindau Nobel Laureate conference 2011 in Germany during the Microsoft sponsored lunch attended by Mr. William H Gates. We made a publication on the



Validation of oral fluid samples to monitor serological changes to Plasmodium Jalciparum by Alexis P Chidi et al. 2011. The project is still ongoing.

In addition to my work on malaria, I co-ordinated a project on schistosomiasis both field work and the laboratory work. The research aim was to assess the prevalence of urinary schistosomiasis among school children in Chitongo area, southern Zambia. My team and I collected urine samples from school children from three randomly selected schools. I performed microscopy on the urine samples together with a second reader for verification as routine. I also wrote a manuscript about this project which was published in the Zambia Medical Journal 2008 edition. During 'this period I was under the direct supervision of Dr Clive Shiff PhD whom I reported to on a daily basis via skype or e-mail. I presented this work at the American Society for Tropical Medicine and Hygiene in December 2008 and at the Zambia National Health Research conference in September 2009. I participated on several other research studies and co-authored abstracts and manual scripts on these projects Mkulama APM, et a12008.

In August 2010, I enrolled in a two year Master's program at the Erasmus MC, University Medical Center, Rotterdam, the Netherlands in the Infection and Immunity program under the faculty of Molecular Medicine. I did my first six months internship in the Hematology department in the group of Dr Tom Cupedo PhD, where I worked on a project to identify the location of human retinoic acid receptor (RAR) related orphan receptor C (RORC) innate lymphoid cells (ILC) in situ. In mice, fetal RORyt+ ILC are involved in development of lymph nodes and in adults RORyH ILC through OX40L and CD30L expression provide survival signals to T cells allowing them to select for B cells during affinity maturation and memory responses. We hypothesized that similar to mouse RORyt+ ILC, human RORC+ ILC are involved in fetal lymphogenesis and postnatal immune responses. Our research aim was to identify the location of RORC+ ILC in human secondary lymphoid organs and to determine whether their location reveals their function. Since organogenesis and immunity research cannot be done in vivo in human we worked with palatine tonsil tissue and human fetal mesenteric lymph nodes. During this work I stained the tissue to locate B-cells, T cells and the stromal cells. In addition I learnt how to perform flow cytometry and make cell cultures. Our results showed that RORC+ ILC in human fetal mesenteric lymph nodes (MLN) are first located at the subcapsular site and during later stages of development are more profound at the paracortical region. Interestingly, in inflamed palatine tonsils, the RORC+ ILC were predominantly localized in the interfollicular areas in close proximity to the B cell follicles, at sites where CXCL13 was expressed by stromal cells. Further analysis revealed that these RORC+ ILC expressed the activation marker NKp44, both on tissue slides and by flow cytometry. We postulated that the data further increases our knowledge on how RORC+ ILC may play a role during lymphoid formation and inflammation, which could lead to better insight in their role in lymphoid organogenesis, inflammation and auto immune diseases.

I recently started my final Master's project which will be the subject ofthesis in the hnmunology department under the direct supervision of Dr Mirjam van der Burg PhD and Dr Gertjan Driessen MD. The project involves determining whether (memory) B cell subsets and vaccination responses can be used to identify primary antibody deficiencies (PAD). Due to genetic heterogeneity of PAD disorders, many patients remain undiagnosed or are diagnosed too



late with poor prognosis. Therefore, based on the new concept regarding the origin of six memory B-cell subsets proposed by Berkowska et al. (2011) I am currently performing a retrospective study and will later conduct a prospective analysis of these subsets in healthy individuals, patients with Common Vmiable Immunodeficiencies (CVID), partial antibody deficiencies (SP AD, IgA, IgG2 deficiency) and patients with recurrent infections without antibody deficiency. We have so far linked the B cell subset results to clinical data retrospectively. The preliminary data show that in healthy individuals, IgG+CD27+ and IgA+CD27+ memory B-cells reach adult levels within two years of age. In CVID and partial antibody deficiencies T-cell independent IgA+CD27- memory B-cell counts were normal. In CVID, the other memory B-cell subsets were decreased. A reduction of IgG+CD27+ B-cells showed the strongest association with severe pneumonia and bronchiectasis. This subset was also decreased in 16% of patients with recurrent infections without antibody deficiency, suggestive of a defect in B-cell development. Currently, we are exploring vaccination responses in relation to the memory B-cell subsets. Besides analyzing the B cell subsets I have participated in diagnostics by extraction of B-cell subsets by flow cytometry and Euroflow and gating the B cells subsets. I have also familiarized myself with culturing fibroblasts, designing primers for genes including CD71 and sequencing the DNA amplicons. This work is still ongoing. This research has been incredibly interesting and has furthered my interest in public health, specifically infectious diseases. I have gained an insight into the function of different genes and the human immune system, and I hope to use that information and some of the techniques in tropical disease research.

With a PhD in Molecular Microbiology and Immunology from Johns Hopkins School of Public Health (JHSPH), I plan to participate in research at both national and international levels. Specifically, I am interested in ongoing efforts of the Centers for Disease Control and the World Health Organization. I plan to continue my research in tropical diseases such as malaria, HIV/AIDS and possibly expand into the field of autoimmune diseases such as cancer. My goal is to be part of a solution to improve the outlook for the millions of individuals struck with deadly infectious diseases I have the opportunity to help through research. I eagerly anticipate gaining the additional knowledge and skills that I need to pursue these ambitions through a PhD program at the JHSPH. A PhD program will enable me to contribute, more fully, to our understanding of infectious diseases and chronic illnesses in my home country Zambia and around the world. My research thus far has prepared me well for the graduate education challenges I now wish to pursue.

Johns Hopkins University Bloomberg Schoo! of Public Health Admissions Services 615 North Wolfe Street Room E1002 Baltimore, MD 21205

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09-Dec-2011

Re~erence#: 2302867/srl Applicant: CH1SHIMBA, Sandra MushHi

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Cc: Sandra Mushili CHISHIMIBA 's-Gravendijkwal 155A Rotterdam ZUID HOLLAND 3021 EM Netherl,ands

Attachments

11111111111111111111111111111111111111111111111 * 3 8 4 3 0 5 3 *

CREDENTIAL EVALUATION AND AUTHENTICATION REPORT

Name: Date of Birth:

CHISHIMBA, Sandra Mushili May 02, 1.981

Date: December 09, 2011 Ref #: 2302867/srl Page: 1 of 4

U.S. EQUIVALENCY SUMMARY

Bachelor's degree from, and one year of graduate study at, a regionally accredited institution

CREDENTIAL ANALYSIS

1. Credential Authentication: Country: Credential: Year: Awarded by: Institution status: Admission requirements: Length of program: Major/Specialization:

U.S. Equivalency:

Official transcripts were sent directly by the institution Zambia Bachelor of Science 2007 University of Zambia Accredited School Certificate Four years Biologica!1 Sciences

Bachelor's degree

CREDENTIAL EVALUATION AND AUTHENTICATION REPORT


CHISHIMBA, Sandra Mushili May 02, 1981

Date: December 09, 2011 Ref #: 2302867/srl

2. Credential Authentication: Country: Credential: Year: Awarded by: Institution status: Admission requirements: Length of program: Major/Specialization:

U.S. Equivalency:

Remarks:

Page: 2 of 4

Official transcripts were sent directly by the institution Netherlands Academic Transcript 2011 Erasmus Universiteit Rotterdam Accredited Bachelor's degree Two years Infection and Immunity

One year of graduate study

Ms. Chishimba is currently enrolled in the final year of a program leading to a Master of Science; anticipated graduation is August 2012.

COU RSE-BY -COURSE ANALYSIS


CH.ISHIMBA. Sandra Mushili May 02,1981

INSTITUTIONS· DATES· SU BJECTS

University of .Zambia 1999·2001 (L) Introductory Chemistry I (L) Mathematical Methods I (L) Cell Biology and Genetics (L) Introductory Physics I (L) Systems Biology (L) Introductory Chemistry II (L) Introductory Physics II (L) Mathematical Methods II 2001·200.2 (L) Cell and Molecular Biology and Genetics (L) Introductory Physics I (L) Form, Function and Diversity of Plants (L) Analytical Chemistry (L) Organic Chemistry I (L) Form, Function and Diversity of Animals (L) Plant and Animal Physiology (L) Introduction to Biochemistry (L) Organic Chemistry II 2003·2004 (L) Introduction to Biochemistry (L) Organic Chemistry II 2004·2005 (L) Cell and Molecular Biology and Genetics (U) Microbiology I (L) Analytical Chemistry (U) Molecular Biology (U) Animal Physiology (U) Microbiology II (U) Biostatistics (U) Parasitology 2005·2006 (U) Molecular Biology (U) Immunology (U) Biochemistry I (Continued on next page)

Date: December 09, 2011 Ref#: 2302867/srl Page: 3 of 4

U.S. Semester U.S. Credits Grades

4.0 C 4.0 C 4.0 C+ (4.0) F* 4.0 C+ 4.0 C 4.0 C+ 4.0 C

(4.0) F* 4.0 C+ 4.0 C+ (4.0) F* 4.0 C 4.0 C+ 4.0 C (4.0) F* (4.0) F*

4.0 B+ 4.0 C

4.0 B 4.0 C 4.0 B (4.0) F* 4.0 B 4.0 C 4.0 C+ 4.0 C+

4 . .0 B 4.0 C+ 4.0 B

COURSE-BY -COURSE ANAL YSIS


CH.ISHIMBA, Sandra Mushili May 02, 1981

INSTITUTIONS - DATES - SUBJECTS

(U) Advanced Parasitology I (U) Advanced Parasitology II (U) Biochemistry II (U) Medica.l Microbiology (U) Food Microbiology 2006·2007 (U) Advanced Molecular Biology (U) Environmenta'i Microbiology (U) Advanced Biochemistry

Erasmus Universiteit Rotterdam 2010-2011

Unspecified Course First Summer Course

Study Design Subject-Related Courses Genetics for Dummies; Statistical Package for the Social Sciences; Public Medicine; Unspecified Course; Drown or Not in Literature; Evaluation Presentations

Foreign Language (English) Biomedical Writing in English

Seminars and Research Discussions Unspecified Course First Winter Course

Biomedical Research Techniques Second Summer Course (unspecified) Extracurricular Studies Mucosal Immunology Symposium and Master Class; Photoshop and Illustrator CS 5; Erasmus MC Liver Day; Systems Biology Applied to Bi%gicallnformation Applications, Sequences and Variations; Erasmus T Cell Consortium

Conference and Presentation in Tropical Medicine and Hygiene Malaria - Epidemiology

Lindau Noble Laureate Meeting

SUMMARY

Total Graduate Semester Credits: Total Undergraduate Semester Credits:

25.0 128 .. 0

GPA: 3.18 GPA: 2.43

Date: December 09, 2011 Ref#: 2302867/srl Page: 4 of 4

U.S. Semester U.S. Credits Grades

4.0 4.0 4.0 4.0 4.0

4.0 4.0 4.0

3.0

2.0 1.0

1.0

6.0 3.0

1.0 3.0 1.0

1.5

2.5

C+ C+ B+ C B

C+ B C

A

A Pass

Pass

Pass B

Pass C Pass

Pass

Pass

THE UNIVERSITY OF ZAMBIA Telephone: 260- 211- 292884 E-mail:··Registrar··<[email protected]> Fax NO.; 260· 211- 253952

P.O. Bo)( 32379 LUSAKA

TO WHOM IT HAY CONCERN Ret: 9845B74

This is to certify that: CHISHIMBA SANDRA M. was a registered student of the University of Zambia in the school of: NATURAL SCIENCES during tbe academic ses&ion (si: 1999/06 Her academic performance was as follows:

1999/01 (Semester I)

CHEMISTRY MATHEMATICS BIOLOGICAL SCIENCES PHYSICS

1999/01 (Semester II)

BIOLOGICAL SCIENCES CHEMISTRY PHYSICS MATHEMATICS


BIOLOGICAL SCIENCES PHYSICS BIOLOGICAL SCIENCES CHEMISTRY CHEMISTRY

101 INTRODUCTORY CHEMISTRY I 111 MATHEMATICAL METHODS I 111 CELL BIOLOGY AND GENETICS 191 INTRODUCTORY PHYSICS ~

112 SYSTEMS BIOLOGY 102 INTRODUCTORY CHEMISTRY II 192 INTRODUCTORY PHYSICS II 112 MATHEMATICAL fl1ETHOlJS 1I

211 CEL~ MOLECULAR BIOLOGY AND GENETICS 191 INTRODUCTORY PHYSICS I 221 FORM, FUNCTION AND DIVERSITY OF PLANTS 225 ANALYTICAL CHEMISTRY I 251 ORGANIC CHEMISTRY I

A key to the unders and~.ng of the g:t"aoes is on the reverse side of this sheet.

DATE: Ol/l1/2011

C C C+ D

c+ C C+ C

D+ C+ C+ D

C

v. M~I\NGWA (MRS.) ACTING DEPUTY REGISTRAR

@638 IIH' 'III"."'"

1.;)':-11 l'1L"m,ar s",IY:;U

RCI,'d Dlf.;lr;:;-Iy ~fDm Illstiillfion

----~------------------

THE UNIVERSITY OF ZAMBIA Telephone: 260~ 21 I· 292884 E-mail: .. Registrar .. <[email protected]> Fax NO.; 260- 211- 253952

P.O. Box 32379 LUSAKA

CHISHIMBA SANDRA M. IS TRANSCRlpT CDNTU. 'Ref. 518458'7'4

2001/02 (Semester It)

BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES CHEMISTRY. CHE1IIJISTRY

2003/04 (Semester IX)

CHEMISTRY CHEMISTRY


BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES CHEMISTRY BIOLOGICAL SCIENCES


BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES

222 FORM FUNCTION AND DIVERSITY OF ANIMALS 212 PLANT AND ANIMAL PHYSIOLOGY 212 INTRODUCTION TO BIOCHEMISTRY 252 ORGANIC CHEMISTRY II

212 INTRODUCTION TO BIOCHEMISTRY 252 ORGANIC CHEMISTRY II

211 CELL MOLECULAR BIOLOGY AND GENETICS 341 MICROBIOLOGY I 225 ANALYTICAL CHEMISTRY I 361 MOLECULAR BIOLOGY

332 ANIMAL PHYSIOLOGY 342 MICROBIOLOGY II 319 BIOSTATISTICS 352 PARASITOLOGY

** CONTINUED ON PAGE 3 **

A key to the understcnding of the grades is on the reverse side of this sheet.

DATE: 01/11/2Q11

C+ C D+ D

B+ C

B C B D

B C C+ C+

A'

,.13- GI T V. M. ~GWA (MRS.) ACTING DEPUTY REGISTRAR

- - - - WGtsTR"AR ~ UNIVERSITY OF ZAMBIA ~ REGISTRARJS DEPARTMENT

P.O. BOX 32379) lUSAKA.

@638 f.~·:d ldL'U!.Clf kt';;l

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THE UNIVERSITY OF ZAMBIA Telephone: 260- 21 Iw 2928M E-mail: .. Registrar.l<[email protected]> Fax NO.: 260- 21 c- 253952 P.O. Box 32379

LUSAKA CHISHIMBA SANDRA M. 's TRANSCRIPT CanTO.


BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES CHEMISTRY BIOLOGICAL SCIENCES


BIOLOGICAL SCIENCES CHEMISTRY BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES

2006/07 (Semeste:t I) .... -.,.._ ..... --BIOLOGICAL SCIENCES BIOLOGICAL SCIENCES CHEMISTRY

361 MOLECULAR BIOLOGY 425 IMQNOLOGY 311 BIOCHEMISTRY 431 ADVANCED PARASITOLOGY

432 ADVANCED PARASITOLOGY 312 BIOCHEMISTRY II 435 MEDICAL MICROBIOLOGY 482 FOOD MICROBIOLOGY

II

441 ADVANCED MOLECULAR BIOLOGY 471 ENVIRONMENTAL MICROBIOLOGY 411 ADVANCED BIOCHEMISTRY I

fief.

I

SHE WAS AWARDED A BACHEIJOR OF SCIENCE DEGREE ON 13TH JULY 2007

~945B74

A key to the underst nding of the grades is on the reverse side of this sheet.

DATE:

B C+ B

C+

C+ B+ C B

C+ B C

•... __ . N, G I ANDU (DR.) . ......"'R ,..... . ,·_ ........ .tr-·....,..c ...... -_

f~ , --~ --.... GlS.TAAR

01/11/2011

V, M'~~GWA (~S,I ACTING DEPUTY REGISTRAR '

~ 1 . IVERSITY OF ZA.MBlA REGISTRAR'S DEPARTMENT P.O. BOX 32379, U:1SAKA •

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Key to understanding of grades of the University ofZamhia

NOTE:

NOTE:

At DISTINCTION

A DISTINCTION

B+ MERlTORIOUS

B VERY SATISFACTORY

S SATISr ACTOR Y

Ct DEFINITE PASS

P PASS

C BARE PASS

CP COMPENSATORY PASS

D+ BARE FAIL

D CLEAR FAIL

E WORTHLESS

SP SUPPLEMENTARY

EX EXEMPTION

IN INCOMPLETE

WP WlTHDRA WN WITH PERMISSION

LT LEFT WITHOUT PERMISSION

(ABANDONED THE COURSE)

NE NOT EXAMINED (ABSENT IN THE

EXAMINATION)

DQ DISQUALIFIED

WD WITHDRA WN WITHOUT PERMISSJON

THIS TRANSCRIPT IS NOT VALID IF IT DOES NOT

BEAR THE UNIVERSITY SEAL OR IF [T HAS ANY

AL TERATJONS.

FOR THE SCHOOL OF MEDICINE

The above key only applies up to fourth year:

Thereafter, the following will apply:

A 72% and above

B 65 ·72%

C 50~64%

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8M Chishimua 's-Gravendijkwal155 A 3021 EM ROTTERDAM

E\ctreft

O(!tum StudiElVoortgangsoverzicht

22 november 2011

347476

Oplejdlng Researchmaster Infection and yollijd Cohort 2010 Immunity

ExamenprQgramma MSCI12010 - Research Master Infection and Immunity

Examenprogramma Minimum Ie

behalen punten Behaalde pUllten VQldlllm

Baslspfogramma

Totnal

pf(~lJrl,lmma

Examenonderdeel

aile onderstasl1de onderdeien

1, Yeflr 1

2, Year 2

3. Elective course$ ,----Totaal

ResultateR • Programma

Onderdeel Cursur;

Year 1 MSCIJ·101 MSCII·102 MSCII·107 MSCII-108 MSCII·109 MSCII-110 MSCII·111 MSCII·112 MSCII·113 MSCII·104 MSCII·105 MSCII·115

Yes.r2 MSCII·201

120.0

120,0

@ ... -"::o':d l ;k:m·.,~ S."lv:::n

HCI}d Gi(~Crly ~rDm I rtSliW~i{ln

39,9 Nee

39,9 Nile

Ty,pe

Verplicht

Verpllcht Verplichte lIeuze

First Summer COLJrse Study Design Genetics for Dummlos SPSS Biomedical English Writing Pubmad Endnote Drown or Not in Literature Evaluation Presentations Seminars, research discussions 1 First Winter COl,lrse Blomodical Research TeChniques

Second Summer Course

Mlnimumt" behalen punte" Behaaldo !punten Voldaan

55,2 33,9 Nee 46,0 6,0 Nee 18,8 0,0 Nee -_ .. _-----

120,0 39,9 Nee

Punten Datum Resultaat ,----

6,0 10-10-2010 9,00 4,3 05-01-2011 7.BO 0.5 01-10·2010 AP 0 .. 8 07-04·2011 AP 2,0 01~O3·2011 AP 0,2 04-01-2011 AP 0,2 05-01-2011 AP 0,2 07-01·2011 AP 0,2 18-02-2011 AP

12,0 12-oe·2011 AP M 11·02·2011 7,20 1,5 15-10-2010 AP ----_.-....---- ...... _ ... 6.0 09·09·2011 6.40

Pagina 1 van

// Universitair;Medisch Centrum Rotterdam

~'t-4~ Immunity

To whom this concerns

Fax 010-703 8015

RoomAe4,13

E·mail f,vanvU"i.@erilsmJ!W1S..n!

Our rtierencenre-

Your reference nee •

Date Tuesday 22 November 2011

Subject: Study results & Letter of recommendation concerning Ms. Sandra Chishimba

L.S.,

I am very much honoured to highly recommend to you Ms. Sandra Chishimba, who is one of out most talented students of the Research master Infection & Immunity at Erasmus MC. Ms. Sandra Chfshlmba has been very active and very successful in many areas, including education and training. She was admitted to the Research Master Infection & Immunity in 2010, a programme specifically designed for excellent students, based on her previous study results and the recommendations we got.

During her training in this Research Master programme she demonstrated her intellectual skills, and is one of the best students of her class, Moreover, she developed her curr,ent research partly by herself. Her research project fits very well within the research projects already running in participating Erasmus Me departments.

The study results overview of Erasmus Me is an overview of the study results of the full time 2 years Rese.arch master study, of Ms. Sandra Chishimba. The total study points she obtained until now is 39,9 + 9,1 (electives) ECTS.

The translation of the words in this study results overview letter is as follows:

Betreft: studievoortgangs-ovel7icht Datum: Opleiding: Voltijd: Examenprogramma; ECTS punten:

Minimum te behalen punten: Behaalde punten: Voldaan: (8asis)programma: Overig: (Examen)onderdeel .. Affe onderstaande onderdelen: Verplicht(e keuze): Nee: Resultaten (over;g):

Subject: study results overview Date: Education program: Full-time Exam program ECTS (European Credit Transfer System) '" study pOints minimal # points to get obtained points completed (Basic) program

Other (Exam) part All paris below: Compulsory (choice) No results (other):

Sdentlflc Director prof. dr. A.D.ME Osterha\Js

Program Director Dr.l.l. Nouwen i·[email protected]

Managing Director Dr. F. va n Vliet [email protected]

Website

www.molmed.nl

, . Date Tuesday 22 November 2011

Curs us: course Deze resultaten tellen niei mae voor dit examenprogramma: these results don't count for this exam program

Above the study results, mentioned in this letter, she also got the next results:

name Dr description of extracurricular e/ect/ll8

4th Symposium 8. Master Classes Mucosal Immunology

Conference & Presentation Am. Soc. Trap. Med. & Hygi€lne: Malaria-Epidemiology

PhQtoshop & Illustrator CS 5

61th Lindau Nobel Laureate Meeting

Erasmus Me liver Day

Systems Biology Applied Bio Info appl's, sequences & variations

1 st Meeting Erasmus T Cel! Consortium

Vet, PhD D' ector Research Master Infection & Immunity

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date (dd-mm-yyyy)

27-05-2011

07-11-2010

13-04-2011

01-07-2011

25-11-2010

29·10·2010

30-03-2011

Admissions Office Student Affairs

A JOHNS HOPKINS ~n~ BLOOMBERG .,.", SCHOOL tfPUBLlC HEALTH

615 North Wolfe Street, Suite EI002


RECOMMENDATION FORM

To the applicant: Please complete the upper portion of the recommendation form and forward it to a person who is familiar with your

professional work, or to a college or graduate faculty member who is acquainted with your academic record.

Applicant's name:--"C:.!.h!!.is~h!!.im,-,-",b""a,----_____ --,S=a,-,n""d.!..:ra",-_______ -'.!M.!.!u""s""h....,i""Ii'--________________ _

Last name First name Middle name Former name(s) if applicable

Social Security No. (U.S.): Email: [email protected]

Degree andlor department to which you are applying: --'P'-'-'hc=O'--"(O=--=o..=cc=.to=-r..=o:.:,f--'P""h:..:.:..oil,;::o,;::s..=o;"cp:.:.h-"'Vu)'---__________________ _

Do you wish to waive your right to examine this letter of recommendation? bZI Yes DNo

To THE PERSON COMPLETING THE RECOMMENDATION FORM:

Please use the reverse side or an attached sheet for your WRITTEN STATEMENT.

A written statement, in addition to the completed chart below, is essential to our evaluation of this applicant. Please rank the applicant

in comparison with others applying for graduate education in the specified degree program.

Signature: Sungano Mharakurwa

Recommended enthusiastically D [ZJ

Name (Print):--"S<-'U ... n""9""a""n!.!.O""-_____ --'M......,h""a""ra ... k.."u""'-'rw ...... a __________ _

Recommended with confidence Title and Department: ~M.:..:..:..:M.:..:I'---____________________ _

Recommended

Recommended with reservation

Not Recommended

D D D

Institution: Johns Hopkins Bloomberg School of Public Health

Address: Malaria Institute at Macha, Namwala Road. Choma, Z

Telephone: +260979312796

Email Address: [email protected]

Once you complete the written statement and this form, please return them promptly to the applicant in the self-addressed envelope that slhe has

provided. PLEASE BE SURE TO SEAL THE ENVELOPE AND SIGN YOUR NAME ACROSS THE SEAL. The applicant has been instructed

to return the sealed recommendation forms along with the rest of the application materials. Thank you for providing this information.

PLEASE USE THE REVERSE SIDE OF THIS SHEET OR AN ATTACHED LETTER FOR YOUR WRITTEN STATEMENT.

GUIDELINES FOR THE WRITTEN STATEMENT MAY BE FOUND ON THE BACK OF THIS SHEET.

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WRITTEN STATEMENT

The School would appreciate a candid statement from you concerning this applicant. In your written statement, please be sure to comment on the following:

How long you have known the applicant and in what capacity

• What you consider to be the applicant's strengths and talents

• Any weaknesses that may impede the applicant's ability to pursue rigorous graduate study

How much thought you feel the applicant has given to study in public health

• English proficiency if the applicant's native language is not English

• The ratings you have assigned on the reverse side of this sheet including any additional comments about the applicant's record, potential, or personal, qualities that you feel would be helpful to the admissions committee

RECOMMENDATION FOR SANDRA CHISHIMBA I have worked with Sandra Chishimba in supervisory capacity for more than four years, from the time she was initially a University of Zambia attachment student till she subsequently joined the Malaria Institute at Macha (MIAM), to the present. From continual review of Sandra's work and academic interaction with her in a scientific team environment, I am familiar with Sandra's intellectual abilities and diligence, andl am pleased that she is applying for her doctoral studies with the JHSPH.

At MIAM, Sandra was exposed to hypothesis-driven research, as well as a broad spectrum of basic immunological, molecular, epidemiological and field research techniques. Subsequent to that, she enrolled for her MSc degree with Erasmus University, The Netherlands, where she has been studying for one year. From personal working experience with Sandra, which encompasses field and laboratory research planning, organization, sample and data collection, management, processing, analysis and presentation, I can attest for her diligence and constant drive learn and improve.

Albeit English is not her native language, it is the official and education language in Zambia. Sandra is fluent in both written and oral expression and has some publications. In my view, Sandra has minimal weaknesses, of which limited exposure to quantitative techniques might be one. However, she learns very quickly and may well have since received training during her MSc studies.

A doctoral degree with JHSPH would be highly instrumental to Sandra's work and the path of her scientific career at MIAM and beyond. I confident that Sandra can successfully undertake her studies and that she will make the most of this invaluable opportunity. She has evidently put careful thought about undergoing training in public health. Hence I recommend her for admission to this strong training programme.

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.HNS HOPKINS Admissions Office Student Affairs rlLOOMBERG

SCHOOLo/'PUBLIC HEALTH 615 North Wolfe Street, Suite ElO02


RECOMMENDATION FORM To the applicant: Please complete the upper portion of the recommendation form and forward it to a person who is familiar with your


Applicant's name:-=C"-h,-"is:e.h:..=.im,-"",b-",a~ _____ --,S=a!..!n-",dc:.;ra,,,--_______ ---,-,M,-,u,,-,s,,-,h-"i-"Ii~ ________________ _



Degree and/or department to which you are applying: _P'--'-'h"'O:....!..'(O=o"'c"'to"'-'-r..::o:..!f--'P....;h'-'.!.!i1-"o.oes"'o"'p:..!h-'-'V .... )c-__________________ _

Do you wish to waive your right to examine this letter of recommendation? [;z] Yes ONo


Please use the reverse side or an attached sheet for your \'lfRITTEN STATEMENT.



Signature: --'-P..::eo.:t=e.:..r ______ ....:A....,.,9L:..r.=e _____ _

Recommended enthusiastically D D D D D

Name (Print):----'-P...,e..,t""e-'-r _______ ----'.A..,.g......,re<--____________ _

Recommended with confidence Title and Department: _________________________ _

Recommended Institution: ______________________________ _

Recommended with reservation Address: _______________________________ _

Not Recommended Telephone: _--'4:....:4c.=3-=-2=8,,-o7_-8=..7'-.4-'..:5"--_________________ _


Once you complete the written statement and this form, please return them promptly to the applicant in the self-addressed envelope that s/he has





25

WRITTEN STATEMENT


26




How much thought you feel the applicant has given to study in public health

English proficiency if the applicant's native language is not English

The ratings you have assigned on the reverse side of this sheet including any additional comments about the applicant's record, potential, or personal qualities that you feel would be helpful to the admissions committee

JOHNS HOPKINS U N V E R S T Y

Bloomberg School of Public Health Department of Molecular Microbiology and Immunology 615 N, Wolfe Street 1 Baltimore MD 21205·2179 443·287·87451 [email protected]

Peter Agre, MD University Professor and Director Johns Hopkins Malaria Research Institute 26 November 2011 Gail O'Connor Sr. Academic Program Coordinator

Dear Colleagues:

It is my pleasure to write on behalf of Sandra Chishimba who is applying for a position as a PhD student in the Molecular Microbiology and Immunology Graduate Program. I am happy to support Sandra and fully believe that she will add to our program in unique ways.

Sandra graduated from the University of Zambia in 2006 and worked as a student at the Malaria Institute at Macha. Despite only average grades as an undergraduate, she performed so well at MIAM that she was hired as a laboratory scientist and has continued to do very good work.

I became well acquainted with Sandra during my visits in 2008,2009, and 2010. She was already a very productive laboratory worker and had a first author publication in 2007 Medical Journal of Zambia reporting on urinary schistosomiasis, while supervised by Clive Shiff. Sandra subsequently had coauthorships on reports describing antifolate resistant malaria, supervised by Sungano Mharakurwa. Sandra is currently enrolled as a masters degree student at Erasmus University in Rotterdam.

Impressed with Sandra, I nominated her as a student delegate to the 2010 Lindau Meeting of the Nobel Laureates in Germany. I was delighted that Sandra was selected among the most outstanding graduate students selected from around the world. Sandra was featured alongside Bill Gates and Nobel Laureate Ada Yonath in the opening plenary session. Sandra's articulate performance indicates major talent in communication of science and may be watched on You Tube video: <http://www.youtube.com/watch ?v= Lfbix4S 7U8c>

I would be very pleased to have Sandra in our program. Please contact me if! may be of any assistance during your deliberations.

Best wishes,

Peter Agre, M.D. University Professor and Director, JHMRI


A JOHNS HOPKINS ~n~ BLOOMBERG '@" SCHOOL o/'PUBLIC HEALTH

615 North Wolfe Street, Suite ElO02


RECOMMENDATION FORM



Applicant's name:---"'C'-'-h'-'.is""-'-'h'-'.im-'-'-"=b""a'--_____ -'S=a!.!n-'='d"'ra"'-_______ -'-!M'-'u"-'s"-'h-'-'i"'Ii'--________________ _



Degree and/or department to which you are applying: _P'---'-'h'-=D'----"(D=o-=c"'to=-r-=o:..:.f..:.P--'h:..:.I:..:·I.::;o.::;s-=o"'p:..:.h~yu)'__ __________________ _

Do you wish to waive your right to examine this letter of recommendation? IZI Yes DNo


Please use the reverse side or an attached sheet for your WRITTEN STATEMENT.



Recommended enthusiastically

Recommended with confidence

Recommended


Not Recommended

D D [Z]

D D

Signarure:-!...P.!.!h"'i1"-'ip"--_____ T-'-'-'h-'='u"'m"'a"----___ _

Name (Print): Philip Thuma

Title and Department: Senior Scientific Advisor

Institution: Macha Research Trust

Address: PO Box 630166. Choma. ZAMBIA

Telephone: +260 977 721 59


Once you complete the written statement and this form, please return them promptly to the applicant in the self-addressed envelope that s/he has





25

WRITTEN STATEMENT


26


What you consider to be the applicant's strengths and talents


I-low much thought you feel the applicant has given to study in public health

English proficiency if the applicant's native language is not English

The ratings you have assigned on the reverse side of this sheet including any additional comments about the applicant's record, potential, or personal qualities that you feel would be helpful to the admissions committee

Written Statement

Macha Research Trust

07 December 2011

Johns Hopkins School of Public Health Admissions Committee

RE: SANDRA CHISHIMBA

doing business as

MALARIA INSTITUTE AT MACHA P.O. Box 630166 Choma, Zambia

Phone/Fax: + 260 21 32 61022 Email: [email protected]

I am delighted to write this letter for Ms Sandra Chishimba, whom I have known for nearly seven years - from the time when she first came to the Malaria Institute at Macha (MIAM) in Zambia as a student on a short attachment.

Sandra was subsequently appointed as a lab scientist for MIAM in January 2007, having graduated from the University of Zambia in 2006. In 2010 she was offered a full scholarship at Erasmus University in The Netherlands to pursue a Master's Degree in Infection and Immunology, so she is currently on study leave from our institute as she pursues those studies.

Her work and personality can be characterized as steadfast, reliable and persistent. Whatever assignment she is given, she puts her whole self into it, and often completes it well ahead of schedule. She does not allow other distractions to divert her attention, and is an extremely task-oriented individual. As a result, she is often given significant responsibilities, since it is well known by her supervisor that she will "do it right"! She learns quickly, but also perseveres if she does not understand something, such that she can achieve most things she sets her mind to do.

Upon joining our research lab in 2007, Sandra not only pursued malaria research work in the molecular biology lab, including polymerase chain reaction (PCR) studies to determine genetic differences in human cases of malaria, but also did work on schistosomiasis with Clive Shiff from Hopkins, including maintaining a snail colony. Before leaving for her master's degree studies, she more recently took a lead role in Bill Moss' ELISA studies on malaria antibody changes over time, as she carried out antibody studies on the specimens collected from local people in this previously hyperendemic malaria transmission area. She presented some of this work at the American Society of Tropical Medicine and Hygiene annual meeting in Atlanta, Georgia, USA in November 2010.

Her first-author publication in the Medical Journal of Zambia (Med J Zambia 34(4):141- 145, 2007) on a study of schistosomiasis, is an example of her commitment after being given an assignment. Not only did she do it whole heartedly, but she then pursued it to make sure a publication resulted from the work.

Philip Thuma

Written Statement

Sandra has an excellent command of spoken and written English, and articulates well in all situations, including public fora.

Having known her now for seven years, I can say that she is definitely committed to a public health approach to disease control, and understands the value of that approach in addition to the need for basic and applied science.

I believe that Sandra's personality and work habits are excellent attributes, and she is an example to other young people in Zambia of what hard work and perseverance can bring. I can recommend her as a suitable candidate for a Ph D degree program at your school.

Yours faithfully,

Philip E. Thuma, M. D. Senior Scientific Advisor, Macha Research Trust (formerly Managing Director of MIAM) also: Senior Research Associate, MMI, JHBSPH

2

Philip Thuma


A JOHNS HOPKINS ~n~ BLOOMBERG ,~" SCHOOL (PUBLIC HEALTH

615 North Wolfe Street, Suite E1002


RECOMMENDATION FORM



Applicant's name:--"C:.:.h~i::::.s,-,h~im:..:.::::b.:::a _______ S=a,-,n.:::d"-,ra,,----_______ -,,M,-,u,,-,s,,-,h-!-'i"'Ii'--________________ _



Degree andlor department to which you are applying: _P,--,-,h",D~(D=o",c"-to""-r-"o'-'.f...!P-,h,-,-,-,-il",o",s,,,o,,,p,-!h.!JY!..J)'----__________________ _

Do you wish to waive your right to examine this letter of recommendation? bZl Yes DNo


Please use the reverse side or an attached sheet for your \'VRITTEN STATEMENT.



Recommended enthusiastically

Recommended with confidence

Recommended


Not Recommended

[Z]

D D D D

Signature:-!.T.",,0:.cm'-'-______ C=u"'p'-'e'-'d"'o"----__ _

Name (Print): Tom Cupedo

Title and Department: PhD, assistant professor. Department of Hematolog

Institution: Erasmus University Medical Center

Address: PO box 2040, 3000CA Rotterdam. The Netherlands

Telephone: +31.10.7044082

Email Address:[email protected]

Once you complete the written statement and this form, please return them promptly to the applicant in the self-addressed envelope that slhe has





25

WRITTEN STATEMENT





• How much thought you feel the applicant has given to study in public health

• English proficiency if the applicant's native language is not English

• The ratings you have assigned on the reverse side of this sheet including any additional comments about the applicant's record, potential, or personal qualities that you feel would be helpful to the admissions committee

Sandra Chishimba joined my laboratory in February 2011 to do a 6 month lab rotation as part of the Masters program Infection and Immunity of the Erasmus University Medical Center.

This was Sandra's first lab rotation in this program. Before pursuing a Masters degree she had been working in her home country of Zambia as part of a research team monitoring humoral immunity to Plasmodium falciparum, and joining my lab meant making the transition from fieldwork and diagnostics to a basic immunology lab. Sandra managed to cope with this transition very well. She was a quick learner and within 2 Months was able to design and analyze experiments independently.

Throughout her internship Sandra maintained a strong interest in translational studies ultimately aimed at improving public health. She remains very passionate about this, and is very eloquent in her ways to convey this passion. As a result, she was invited to the 2011 Lindau meeting of Nobel Laureates, where she was invited to take part in a forum discussion on Malaria together with Bill Gates, Nobel Laureate Ada Yonath (Chemistry 2009) and Jonathan Carlson (Microsoft Research).

During her internship we discussed her future perspectives, and at that time she already indicated that she wanted to pursue a PhD in medical sciences, preferably again in another country. Sandra's scientific drive and clear ideas on future directions exceed those of many of her fellow students. She enjoys cultural differences, is capable of handling new situations and environments very well and it is my strong belief that Sandra will develop into a successful independent scientist with a broad interest, yet strong translational focus.

I would very much recommend Sandra for the Johns Hopkins Bloomberg School of Public Health PhD program. I am convinced that she will be a valuable addition to the student body, and that she will progress into a successful scientist.

Sincerely,

Tom Cupedo, PhD Erasmus University Medical Center Department of Hematology Rotterdam, the Netherlands

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