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Table of Contents

SURF 2016 Call for Abstracts 2

General Abstract Submission Guidelines 3

Four Presentation Formats

Paper and Poster Presentations, and Roundtable Discussion Abstract Instructions

5

Online Abstract Submission for Paper and Poster Presentations, and Roundtable Discussion

5-6

Program Booklet Abstract Formatting Instructions for Paper, Poster, and Roundtable Discussion Word Document Files 6-7Evaluation Criteria for Paper, Poster, and Roundtable Discussion Abstracts

7-8

Symposia 9

Abstract Instructions for a Symposium 9Online Abstract Submissions for a Symposium 10-11Abstract Formatting Instructions for Symposium Word Document Files

11

Evaluation Criteria for Symposium Abstracts 12

Abstract Submission Timeline 13

SURF 2016 Website Links 13

SURF Registration and Parking Information 13

Contact Information 13

Appendices 14Appendix A: US Military Rank Abbreviations 15-17Appendix B: Abstract Template for Paper, Poster, and Roundtable Discussion

18

Appendix C: Sample Abstract with Authors from Multiple Institutions

19

Appendix D: Sample Abstract with Authors from the Same Institution

20

Appendix E: Sample Abstract with Multiple Authors, Including a 21

Last Updated January 15, 2016

Military AffiliationAppendix F: Abstract Template for Symposium 22


SURF 2016 CALL FOR ABSTRACTS

EXTENDED: Online Abstract Submission DeadlineFriday, February 5, 2016, 11:59 P.M. CST

San Antonio is a unique city, serving as a leader in government and civilian healthcare research through organizations like the San Antonio Military Health System (SAMHS), The University of Texas Health Science Center at San Antonio (UTHSCSA), and The University of Texas at San Antonio (UTSA). These institutions invite you to the second annual SAMHS and Universities Research Forum (SURF), a local event to showcase the work of trainees, faculty, staff and students from multiple disciplines and public health agencies across San Antonio. The forum will be held on Friday, May 20, 2016, at The University of Texas at San Antonio Main Campus, H-E-B University Center Ballroom (HUC 1.104) from 8:00 A.M. - 5:00 P.M. Registration opens at 7:00 A.M. Registration and parking are free.

With the theme “Evidence-Based Practice & Research in Healthcare: Encouraging Collaborative Partnerships”, forum participants will have the opportunity to present and share research, practices, policies, and collaborations; earn continuing education credits/units (e.g., CME, CNE); and connect with scholars and practitioners from SAMHS, academic institutions, and public, government, and private organizations and agencies for future education, research, policy, and practice collaborations.

Research, practices, policies, and collaborations will be shared in paper and poster sessions, roundtable discussions, and symposia along eleven focus areas/learning tracks:

Pre-Hospital Trauma Inpatient and Outpatient Care Technology and Innovation in Healthcare Preventative Medicine Personalized Medicine (Precision Medicine) Public Health/Wellness Patient Safety Regenerative Medicine Medical Modeling and Simulation Policy, Practice, and Collaborations Open: This category is open. Author’s work is related to protecting,

maintaining, and improving health.


General Abstract Submission GuidelinesWe invite abstracts for research, policy, practice, and collaboration paper and poster presentations, roundtable discussions, and symposia directly connected to at least one of the eleven focus areas/learning tracks. Submit abstracts through the SURF 2016 online abstract submission website by Friday, February 5, 2016, at 11:59 P.M. CST.

Accepted participants will be notified via email on February 19, 2016. Invitations must be accepted or declined by February 26, 2016. In addition to submitting an abstract, all SURF participants must register to attend SURF via the SURF 2016 registration website.

Instructions for paper, poster, roundtable discussion, and symposium abstracts are provided in this guidebook. While each presentation format has a particular set of guidelines, the following are required for each abstract submission:

Abstract without identifying information for reviewers to evaluate MSWord file for program booklet abstract *CV/resume from author: If there are multiple authors, only one

CV/resume must be submitted *Completed disclosure form from author: If there are multiple

authors, only one disclosure form must be submitted *Three educational/learning outcomes

*SURF provides continuing education credits. This information is used to offer continuing education credits and will not be used to evaluate abstract submissions.


https://www.regonline.com/SURF2016

https://easychair.org/conferences/?conf=surf2016


Four Presentation FormatsPaper Presentation There are different types of paper presentations. They include: (1) an oral summary of a completed research study of an empirical or theoretical nature; (2) an essay that presents well-developed arguments on philosophical, theoretical, or practical problems; (3) program evaluation; and (4) policy analysis.

Poster PresentationPoster presentations provide a forum for scholars and practitioners to engage in active discussion with other forum participants about a completed or developing project focused on research, practice, policy, and collaboration. The poster venue allows scholars and practitioners with similar interests to interact by using the poster as a focal point.

Roundtable DiscussionRoundtable discussions provide opportunities for authors to share information in an informal, conversational style. Presenters are assigned to numbered tables in a large meeting room, where interested persons gather for discussion with the presenter.

In the roundtable session, authors should allocate roughly equal time between discussion of their research, practice, policy, or collaboration and opportunities for questions from those who attend the session. Roundtable sessions do not include a discussant; therefore, it is the responsibility of the author(s) to encourage and allow time for all attendees to participate in the discussion. Given the informal structure of the roundtable presentation, no audiovisual equipment will be provided.

SymposiumA symposium is a thematic, integrated, and interactive engagement between a panel of presenters and the audience. The focus of the discussion is a clearly identified theme or topic that: (a) utilizes a particular disciplinary or theoretical perspective in considering a research problem; (b) reflects an emerging issue in a specific area of research, policy, or practice; or (c) centers on the development of health-related theory or research methodology.

The symposium organizers determine the theme and set of presenters for an entire session. Symposia should not be organized in the same format as a traditional research paper session, but facilitate a session for those interested in enhancing practice, research, policy, and/or collaborations in healthcare. Members of the panel will make brief presentations linking their talking points to the theme, after which ample opportunity should remain for discussion. The discussion should be facilitated by a moderator.


Paper and Poster Presentations, and Roundtable Discussion Abstract Instructions

All paper, poster, and roundtable discussion abstracts must be submitted online via the SURF 2016 EasyChair website. For those who have not used EasyChair, you will be prompted to set-up an EasyChair account in order to submit your abstract(s).

The online form includes two versions of the abstract: (1) a 500-word abstract posted on the online form and (2) an uploaded one-page Word document file. These two abstract versions vary. The latter includes identifying information while the former does not. Reviewers will not have access to the Word document file. For accepted abstracts, the Word document file will appear in the forum program booklet.

Online Abstract Submission for Paper and Poster Presentations, and Roundtable Discussions

All paper, poster, and roundtable discussion abstracts must be submitted online via the SURF 2016 EasyChair website. On the submission page, you will provide/designate/upload the following information:

o Author(s) Information: This includes: first and last name, email, country, organization (list department and institutional affiliations), web site (if applicable), and designate the corresponding author(s).

o Title

o Abstract: No more than 500 words.

o Keywords: Provide at least three key phrases or key terms that characterize/describe your abstract submission.

o Focus Areas/Learning Tracks: Select the appropriate focus area/learning track.

o Presentation Format: Indicate if the abstract is for a paper presentation, poster presentation, or roundtable discussion.

o Presentation Objectives: List no less than three educational objectives/outcomes. An educational objective describes what a participant will learn by participating in or attending your presentation/discussion. List three objectives/outcomes (e.g., Participants will become aware of....).




o Abstract for Program Booklet—Upload Word Document File: See formatting instructions in the next section and appendices for a template formatting guide, sample abstracts, and military rank abbreviations. NOTE: Reviewers will not have access to this file. This version of the abstract will appear in the program booklet.

o Resume/CV—Upload PDF File: Upload one CV/resume as a PDF file. If there are multiple authors, only one author CV/resume should be submitted. NOTE: Reviewers will not have access to this file.

o Continuing Health Education Activity Disclosure Form—Upload PDF File: Upload one completed Continuing Health Education Activity Disclosure Form as a PDF file. If there are multiple authors, only one of the authors must complete and submit the disclosure form for the group. The website link for the disclosure form:

http://research.utsa.edu/wp-content/uploads/2015/06/CHEA_DisclosureForm.doc

NOTE: Reviewers will not have access to this file.

When the online submission form is completed, press the red “Submit” button. The corresponding author(s) will receive a confirmation email from EasyChair.

Program Booklet Abstract Formatting Instructions for Paper, Poster, and Roundtable Discussion Word Document Files The uploaded Word document file for paper, poster, and roundtable discussion abstracts should be formatted as follows:

o Title: Arial font, 14-point size, centered, boldface, all caps, and two spaces between each word

o Author(s): Use Arial font, 12 point-size, centered, and boldfaced with presenter(s) name first. Provide First and Last names followed by a comma and then the abbreviation for academic credential (e.g., Jill Hernandez, Ph.D. 1). Use superscript after the academic credential to reference departmental and institutional affiliations (e.g., First Name Last Name, Degree1, First Name Last Name, Degree2, and First Name Last Name, Degree3). If author has a military title, place military rank


abbreviation according to military branch (see chart on pages 15-17) before the first name (e.g., LtCol Vikhyat S. Bebarta, MD1).

o Department and Institutional Affiliations: Use superscripts if there are multiple institutions and affiliations (e.g., 1Department Name, College Name, Institution Name; 2Department Name, College Name, Institution Name; and 3Department Name, College Name, Institution Name). Here is another example: 1Department of Biology, College of Sciences, The University of Texas at San Antonio; and 2Department of Anatomy & Cell Biology, East Carolina University. If authors have the same department and institutional affiliations, they can use the same superscript. See abstract examples in the appendix.

o Abstract Body: Arial font, 12-point size, use 1” page margins on all sides. Indent the first line of each paragraph by half an inch. Use both left and right justification. Superscripts and subscripts should be 9-point size.

o The abstract is limited to one full page (with title & authors). All content must be in black and white. Abstracts may, but are not required to, include representative figures, tables, or images. You are encouraged to format the body of the abstract to include the following sections: Background, Materials & Methods, Results, and Conclusions. NOTE: Your Word document file may exceed the one-page limit if your author and acknowledgement/funding information is extensive. If so, please keep the body of the abstract (e.g., background, materials & methods, results, and conclusions) to 500 words and follow the font and formatting instructions.

o Please include acknowledgements and funding support. NOTE: If this information limits the size of abstract body, please extend the text to an additional page.

o You are required to use this template for your abstract submission.

Please refer to Appendices for an abstract template, examples of program booklet abstracts, and a chart of US Military Rank abbreviations.

Reviewers will not have access to the uploaded files. For accepted abstracts, the Word document file will appear in the forum booklet.

Evaluation Criteria for Paper, Poster, and Roundtable Discussion AbstractsEach paper, poster, and roundtable discussion abstract must demonstrate:


Relevance/Importance to at least one of the eleven focus areas/learning tracks: Pre-Hospital Trauma; Inpatient and Outpatient Care; Technology and Innovation in Healthcare; Preventative Medicine; Personalized Medicine (Precision Medicine); Public Health/Wellness; Patient Safety; Regenerative Medicine; Medical Modeling and Simulation; Policy, Practice, and Collaborations; and Open.

Technical Quality: Demonstrate clear ideas and arguments addressing theory, practices, and/or methods. Exhibit high quality writing and accurately cite relevant sources.

Innovation: Introduce and/or promote the development of new ideas, practices, and/or methods within a selected focus area/learning track.

Intellectual Merit: Research advances knowledge and understanding within a field of study or across disciplines.

Broader Impacts to Society: Research must not only contribute to the growing fund of knowledge, but benefit society, including teaching, training, learning, and outreach.


SymposiaA symposium is a thematic, integrated, and interactive engagement between a panel of presenters and the audience. The focus of the discussion is a clearly identified theme or topic that: (a) utilizes a particular disciplinary or theoretical perspective in considering a research problem; (b) reflects an emerging issue in a specific area of research, policy, or practice; or (c) centers on the development of health-related theory or research methodology.

The symposium organizers determine the theme and set of presenters for an entire session. Symposia should not be organized in the same format as a traditional research paper session, but facilitate a session for those interested in enhancing practice, research, policy, and/or collaborations in health and healthcare. The names of presenters should be included in the Word document file, and the backgrounds of presenters should be described. Members of the panel will make brief presentations linking their talking points to the theme, after which ample opportunity should remain for discussion. The discussion should be moderated, and a moderator should be designated within the Word document file.

A symposium submission highlights (1) objectives of the session; (2) significance of the theme or topic; (3) identification of the presenters and a discussion of their backgrounds related to the theme or topic; (4) a discussion of the research or perspective that each presenter will contribute; and (5) a discussion of how the session will be structured, including designating the moderator and strategies to facilitate audience discussion.

Symposium Word document files will be evaluated on the following criteria: (1) originality; (2) clarity of focus; (3) significance of topic and/or relevance to health research, practice, policy, and/or collaboration; (4) integration and coherence of panel’s presentations as a group; (5) range of knowledge and/or points of view represented by panelists; (6) adequacy of strategy for involving audience in the discussion and for making the session interactive; and (7) ways that the session intentionally tries to facilitate learning among participants.

Abstract Instructions for SymposiaAll symposium abstracts must be submitted online via the SURF 2016 EasyChair website. For those who have not used EasyChair, you will be prompted to set-up an EasyChair account.

The online form includes two versions of the abstract: (1) a 500-word abstract posted on the online form and an extended uploaded Word document file. These two abstract versions vary. The latter is longer and includes identifying information while the former does not. Reviewers will evaluate the Word document file. For accepted symposium abstracts, the




online 500-word abstract and the presenter and moderator identifying information from the Word document file will appear in the forum program booklet.


Online Abstract Submissions for Symposia All symposium abstracts must be submitted online via the SURF

2016 EasyChair website. On the submission page, you will provide/designate/upload the following information:

o Author(s) Information: This includes: first and last name, email, country, organization (list department and institutional affiliations), web site (if applicable), and designate the corresponding author(s).

o Title

o Abstract: This abstract will not be evaluated, but will be published in the program booklet. In no more than 500 words the symposium abstract should summarize: (1) objectives of the session and (2) significance of the theme or topic.

o Keywords: Provide at least three key phrases or key terms that characterize/describe your abstract submission.

o Focus Areas/Learning Tracks: Select the appropriate focus area/learning track.

o Presentation Format: Indicate the abstract is for a symposium.

o Presentation Objectives: List no less than three educational objectives/outcomes. An educational objective describes what a participant will learn by participating in or attending your presentation/discussion. List three objectives/outcomes (e.g., Participants will become aware of....).

o Abstract for Program Booklet—Upload Word Document File: See formatting instructions in the next section and appendices for a template formatting guide, sample abstracts, and military rank abbreviations. NOTE: Reviewers will evaluate this file. This version of the abstract will not appear in the program booklet. However, the information about the moderator and panelists will be used printed in the program booklet.

o Resume/CV—Upload PDF File: Upload one CV/resume as a PDF file. Since there are multiple presenters/facilitators, only one presenter CV/resume should be submitted. NOTE: Reviewers will not have access to this file.




o Continuing Health Education Activity Disclosure Form—Upload PDF File: Upload one completed Continuing Health Education Activity Disclosure Form as a PDF file. Since there are multiple presenters, only one of the presenters must complete and submit the disclosure form for the group. The website link for the disclosure form:


NOTE: Reviewers will not have access to this file.

When the online submission form is completed, press the red “Submit” button. The corresponding author(s) will receive a confirmation email from EasyChair.

Abstract Formatting Instructions for Symposium Word Document FilesThe uploaded Word document file for symposium submissions should be formatted as follows:

o Title: Arial font, 14-point size, centered, boldface, all caps, and two spaces between each word

o Presenter(s) and Moderator and Their Background: Use Arial font, 12 point-size, centered, and boldfaced with presenter(s) name first. Provide first and last names, credentials, department, college/school (if applicable), and institutional affiliations (e.g., Jill Hernandez, Ph.D., Biomedical Engineering, College of Sciences, The University of Texas at San Antonio). Place military rank abbreviation before the first name. See military rank abbreviation chart on pages 15-17. Include 2-5 sentences that discuss each presenter’s background related to the theme or topic.

o Abstract Body: Arial font, 12-point size, use 1” page margins on all sides. Indent the first line of each paragraph by half an inch. Use both left and right justification. Superscripts and subscripts should be 9-point size. In no more than 1,000 words the symposium description should include as many of the following as are applicable, preferably in this order: (1) objectives of the session; (2) significance of the theme or topic; (3) a discussion of the research or perspective that each presenter will contribute; (4) a discussion of how the session will be structured, including the and strategies to facilitate audience discussion.

o Please include acknowledgements and funding support.


o You are required to use this template for your symposium Word document file submission.

o Reviewers will evaluate this symposium Word document file. For accepted abstracts, the online 500-word abstract and the presenter and moderation information from the Word document file will appear in the forum program booklet.

Evaluation Criteria for Symposium AbstractsSymposium Word document files will be evaluated according to the following criteria:

Originality

Clarity of focus

Significance of topic and/or relevance to health research, practice, policy, and/or collaboration

Integration and coherence of panel’s presentations as a group

Range of knowledge and/or points of view represented by panelists

Adequacy of strategy for involving audience in the discussion and for making the session interactive

Ways that the session intentionally tries to facilitate learning among participants.


Abstract Submission Timeline Monday, November 16,

2015Online abstract submission opens

Friday, February 5, 2016, at 11:59 P.M. CST

Extended Deadline to submit abstract(s)

Friday, February 19, 2016 Accepted abstract notification

Friday, February 26, 2016 Deadline to accept/decline presentation invitation(s)

Friday, May 20, 2016 SURF 2016

SURF 2016 Website Links SURF Website: http://research.utsa.edu/surf/ Abstract Submission: https://easychair.org/conferences/?

conf=surf2016 Continuing Health Education Activity Disclosure Form:


Registration: https://www.regonline.com/SURF2016 . NOTE: To complete your online registration, do not use Internet Explorer. Please use another browser.

SURF Registration and Parking InformationSURF registration and parking are free to all participants. All presenters must register to attend SURF at this website: https://www.regonline.com/SURF2016

Free parking is provided in Brackenridge Avenue Lots 1 & 2. No permit is required. If you would like to park closer to the H-E-B University Center (HUC), parking is available in the Ximenes Avenue Garage, which charges $2.00/hour. See the SURF-UTSA Campus map: http://research.utsa.edu/surf/download/SURF_Map(2).pdf.

Contact InformationFor assistance with the SURF abstract submission, please contact Victor Sylvia, Ph.D., Senior Scientist, Contractor, Office of the Chief Scientist, 59th

MDW/ST, Joint Base San Antonio—Lackland, TX, via email ([email protected]) or phone (210-292-3513).


http://research.utsa.edu/surf/download/SURF_Map(2).pdf





http://research.utsa.edu/surf/

For additional information about SURF, including registration, please contact Jaclyn Shaw, Director for Research Support, The University of Texas at San Antonio, via email ([email protected]) or phone (210-458-6767).


Appendices


Appendix A: US Military Rank Abbreviations

Air Force - NCO's and Officers

Rank Abbrev. Rank Abbrev.Airman Basic AB Chief Master Sergeant

of the Air Force CMSAFAirman Amn Second Lieutenant 2d Lt

Airman First Class A1C First Lieutenant 1st LtSenior Airman SrA Captain CaptStaff Sergeant SSgt Major Maj

Technical Sergeant TSgt Lieutenant Colonel LtColMaster Sergeant MSgt Colonel ColFirst Sergeant 1stSgt Brigadier General BrigGen

Senior Master Sergeant SMSgt Major General MajGenFirst Sergeant 1stSgt Lieutenant General LtGen

Chief Master Sergeant CMSgt General GenFirst Sergeant 1stSgt General of the Air Force

Command Chief Master Sergeant CCMSgt

Army - NCO's and Officers

Rank Abbrev. Rank Abbrev.Private PVT Chief Warrant Officer 3 WO3Private PV2 Chief Warrant Officer 4 WO4

Private First Class PFC Master Warrant Officer 5 WO5Specialist SPC Second Lieutenant 2LTCorporal CPL First Lieutenant 1LTSergeant SGT Captain CPT

Staff Sergeant SSG Major MAJSergeant First Class SFC Lieutenant Colonel LTC

Master Sergeant MSG Colonel COLFirst Sergeant 1SGT Brigadier General BGSergeant Major SGM Major General MG

Command Sergeant Major CSM Lieutenant General LTGSergeant Major of the Army SMA General GEN

Warrant Officer WO1 General of the ArmyChief Warrant Officer 2 WO2


Coast Guard - NCO's and Officers

Rank Abbrev. Rank Abbrev.Seaman Recruit SR Chief Warrant Officer CWO-3 CWO-3

Seaman Apprentice SA Chief Warrant Officer CWO-4 CWO-4Seaman SN Ensign ENS

Petty Officer Third Class PO3 Lieutenant Junior Grade LTJGPetty Officer Second Class PO2 Lieutenant LT

Petty Officer First Class PO1 Lieutenant Commander LCDRChief Petty Officer CPO Commander CDR

Senior Chief Petty Officer SCPO Captain CAPTMaster Chief Petty Officer MCPO Rear Admiral (Lower Half) RADM

(LH)Command Master Chief Petty

Officer MCPOC Rear Admiral (Upper Half) RADM (UH)

Master Chief Petty Officerof the Coast Guard

MPCO-CG Vice Admiral VADM

Chief Warrant Officer CWO-2 CWO-2 Admiral ADM

Marine Corps - NCO's and Officers

Rank Abbrev. Rank Abbrev.Private Pvt Chief Warrant Officer 3 CWO-3

Private First Class PFC Chief Warrant Officer 4 CWO-4Lance Corporal LCpl Chief Warrant Officer 5 CWO-5

Corporal Cpl Second Lieutenant 2ndLtSergeant Sgt First Lieutenant 1stLt

Staff Sergeant SSgt Captain CaptGunnery Sergeant GySgt Major MajMaster Sergeant MSgt Lieutenant Colonel LtColFirst Sergeant 1stSgt Colonel Col

Master Gunnery Sergeant MGySgt Brigadier General BGenSergeant Major SgtMaj Major General MajGen

Sergeant Major of the Marine Corps

SgtMajMC Lieutenant General LtGen

Warrant Officer WO-1 General GenChief Warrant Officer 2 CWO-2


Navy - NCO's and Officers

Rank Abbrev. Rank Abbrev.Seaman Recruit SR Chief Warrant Officer Fourth Class CWO4

Seaman Apprentice SA Chief Warrant Officer CWO5Seaman SN Ensign ENS

Petty Officer Third Class PO3 Lieutenant Junior Grade LTJGPetty Officer Second Class PO2 Lieutenant LT

Petty Officer First Class PO1 Lieutenant Commander LCDRChief Petty Officer CPO Commander CDR

Senior Chief Petty Officer SCPO Captain CAPTMaster Chief Petty Officer MCPO Rear Admiral (Lower Half) RDML

Master Chief Petty Officer of the Navy MCPON Rear Admiral (Upper Half) RADM

Warrant Officer First Class WO1 Vice Admiral VADMChief Warrant Officer Second

Class CWO2 Admiral ADMChief Warrant Officer Third Class CWO3 Fleet Admiral FADM


Appendix B: Abstract Template for Paper, Poster, and Roundtable Discussion

TITLE: A R I A L F O N T , 1 4 POINT-SIZE, CENTERED, BOLDFACE, ALL CAPS, TWO SPACES BETWEEN

WORDS

Authors: Arial font, 12 point-size, centered and boldfaced with presenter(s) name first. Provide First and Last names followed by a comma and then abbreviation

for academic credential. Use superscript after the academic credential to reference departmental and institutional affiliations (e.g., First Name Last Name, Degree1, First Name Last Name, Degree2, and First Name Last Name, Degree3). If

author has a military title, use military rank abbreviation before the first name (see chart on pages 15-17).

Department and institutional affiliations: Use superscripts if there are multiple institutions and affiliations. If authors have the same departmental and

institutional affiliations, they can use the same superscript (e.g., 1Department Name, College Name, Institution Name; 2Department Name, College Name, Institution Name; and 3Department Name, College Name, Institution Name)

Abstract Body: Arial font, 12-point size, use 1” page margins on all sides. Indent the first line of each paragraph by half an inch. Use both left and right justification. Superscripts and subscripts should be 9-point size.

The abstract is limited to one full page (with title & authors). All content must be in black and white. Abstracts may (but are not required) to include representative figures, tables, or images. You are encouraged to format the body of the abstract to include the following sections: Background, Materials & Methods, Results, and Conclusions.

Please inc lude acknowledgements and funding support . You are required to use this template for your abstract submission.


Appendix C: Sample Abstract with Authors from Multiple Institutions

TSPAN8 EXPRESSION DISTINGUISHES SPERMATOGONIAL STEM CELLS FROM PROGENITORS AMONG NEONATAL MOUSE

UNDIFFERENTIATED SPERMATOGONIA

Brian P. Hermann, Ph.D.1, Thu Nguyen, B.S.1, Kazadi Mutoji, Ph.D.1, Christopher B. Geyer, Ph.D.2, Amy Kaucher, B.S.3, John R. McCarrey, Ph.D.1, and Jon M. Oatley, Ph.D.3

1Department of Biology, College of Sciences, The University of Texas at San Antonio; 2Department of Anatomy & Cell Biology, East Carolina University; and 3Center for

Reproductive Biology, Washington State University

Mammalian spermatogenesis is maintained by self-renewal and differentiation of spermatogonial stem cells (SSCs), which produce progenitor spermatogonia that have a finite replicative lifespan and will generate the remaining germ cells in the spermatogenic lineage. SSCs and progenitors together comprise the undifferentiated spermatogonial pool, but no single molecular feature has been shown to distinguish these functionally-distinct cell populations. We previously demonstrated existence of subpopulations of undifferentiated spermatogonia based on gene expression heterogeneity at the single-cell level. Twenty-seven of the 172 examined genes were expressed in a bimodal fashion among Id4-eGFP+ spermatogonia (i.e., present/absent), suggesting they mark two or more discrete cell subpopulations of undifferentiated spermatogonia.

Flow cytometry with antibodies against cell surface proteins (TSPAN8, EPHA2, and PVR) encoded by three of these bimodal mRNAs demonstrated that 29.87 ± 2.57% of Id4-eGFP+ cells were positive for only one of three surface proteins while 30.37 ± 7.09% were positive for two markers. In both cases, PVR staining was predominant. Co-localization of all three markers was observed in 14.44 ± 8.21% of P6 Id4-eGFP+ cells and 25.32 ± 13.03% were triple-negative. Subsequent cell sorting experiments demonstrated that mRNA levels of other bi-modally expressed genes were enriched in TSPAN8 high and EPHA2 high subpopulations of Id4-eGFP+ cells. Further, transplantation studies demonstrated enrichment of SSCs in the TSPAN8-high population of P6 Id4-eGFP+ cells. Together, these studies provide additional support for the notion that there are discrete subpopulations of undifferentiated spermatogonia in the neonatal mouse testis and demonstrate that these subpopulations have differing functional capacities. This information will help identify the elusive SSC in human testes and provide a means of characterizing cells intended for therapeutic SSC transplantation for treating infertility.

This study was supported by NIH grants HD062687 (BPH), HD061665 (JMO), HD072552 (CBG), and GM092334 (JRM), NSF grant 1337513 (BPH), the Max and Minnie Tomerlin Voelcker Fund, the Helen Freeborn Kerr Charitable Foundation, the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation, and The University of Texas at San Antonio.


Appendix D: Sample Abstract with Authors from the Same Institution

IDENTIFICATION OF A NOVEL THERAPEUTIC TARGET FOR TREATMENT OF GASTROINTESTINAL COLONIZATION WITH MULTI-

DRUG RESISTANT ACINETOBACTER BAUMANNII

Patrick Ketter, MB, MLS (ASCP)cm, M. Neal Guentzel, Ph.D., Rishein Gupta, Ph.D., Jieh-Juen Yu, Ph.D., James Chambers, Ph.D., and Bernard Arulanandam, Ph.D.

Department of Biology, College of Sciences, The University of Texas at San Antonio

Background and Significance: Acinetobacter baumannii is among the most common causes of combat related infections in recent history. Ubiquitous in nature and resistant to desiccation, this organism is also of major concern for nosocomial settings. Furthermore, multi- and extreme-drug resistance, linked to gastrointestinal (GI) colonization, has created difficulties in treatment and control of these infections, increasing risk of sepsis. Recently, studies indicate A. baumannii is capable of degrading secretory IgA (SIgA), the primary humoral immune defense at mucosal surfaces, the mechanism and purpose of which is poorly understood.

Methods: The in vivo contribution of SIgA to bacterial colonization was assessed using WT, IgA-/- and pIgR-/- KO mice through in vivo live imaging assays. Using protease and thiol-reductase inhibitors, we examined the mechanism of SIgA degradation by A. baumannii. Intestinal sections collected from WT, IgA-/- and pIgR-/- KO mice were used to assess contributions of SIgA and secretory component (SC) to intestinal adherence of this bacterium. Finally, Modulation of A. baumannii mRNA gene transcripts corresponding to proteins involved in disulfide bond reduction were assessed through RNAseq and confirmed by RT-qPCR following SIgA exposure.

Results: In vivo imaging assays revealed WT mice with normal SIgA levels exhibited greater persistence of A. baumannii in the GI track following oral gavage compared to IgA-/- and pIgR-/- KO mice. Additionally, degradation of SIgA by A. baumannii was significantly reduced following inhibition of thiol-reductase activity. Intestinal section assays also revealed both the lack of SIgA and inhibition of its degradation by A. baumannii significantly decreased bacterial intestinal adherence. Finally, thioredoxin-A, a gene implicated in immunoglobulin degradation in other bacterial systems, was found to be up-regulated following SIgA exposure by RNAseq and RT-qPCR analyses.

Conclusions: SIgA enhances A. baumannii GI colonization. Additionally, use of a thiol-reductase inhibitor to prevent SIgA degradation by A. baumannii significantly reduces bacterial adherence to the intestinal epithelium indicating a potential drug target to prevent colonization and emergence of additional drug resistance.


Appendix E: Sample Abstract with Multiple Authors, Including a Military Affiliation

PREHOSPITAL AND EN ROUTE CRICOTHYROTOMY PERFORMED IN THE COMBAT SETTING: A PROSPECTIVE, MULTICENTER,

OBSERVATIONAL STUDY

Edward B. G. Barnard, FCEM1,2, Alicia T. Ervin, RN1, Robert L. Mabry, MD3, and LtCol Vikhyat S. Bebarta, MD1

1Air Force En route Care Research Center, US Army Institute of Surgical Research/59th MDW; 2Institute of Naval Medicine, UK; 3Joint Trauma System, Center of Excellence, US Army

Institute of Surgical Research

Background: Airway compromise is the third commonest cause of potentially preventable combat death. Surgical cricothyroidotomy (CRIC) is an infrequently performed but life-saving airway intervention. There is limited published prehospital data on prehospital CRIC. The aim of our study was to describe the survival rate and complications associated with cricothyroidotomy performed in the military prehospital and en route setting.

Methods: The Life Saving Intervention (LSI) study is a prospective, IRB approved, multicenter trial examining prehospital combat LSIs. Trained site investigators recorded demographics, vital signs, and LSIs performed. Predefined LSIs included cricothyroidotomies, chest tubes, intubations, and tourniquets. Descriptive statistics or Wilcoxon test (non-parametric) were used for data comparisons.

Results: Of the 1,927 patients enrolled, 34 patients had a CRIC performed (1.8%). Median age for patients with a CRIC was 24 (IQR 22.5-25), 97% male. Mechanisms of injury were blast (79%), penetrating (18%), and blunt (3%); 83% had major head and or facial injuries. Median GCS was 3 (IQR 3-7.5] and 7 patients had GCS > 8. CRIC was successful in 82% of cases. Reasons for failure included left main stem intubation (1), subcutaneous passage (1), and unsuccessful attempt (4). Unsuccessful endotracheal intubation preceded 15% of CRICs. Of the patients who had the provider type recorded (n=24), six had CRICs by a combat medic (pre-evacuation), and 18 by an evacuation helicopter medic. 52% (n=13) survived to hospital discharge. The patients with CRICs had more LSIs than non-CRIC patients (4 versus 2 LSIs/patient, p<0.0011).

Conclusion: In our prospective, multicenter study, evaluating cricothyroidotomy in a prehospital and en route care combat setting, survival was 52%, higher than previously reported. In addition, most cricothyroidotomies were performed by the evacuation helicopter medic rather than the prehospital combat medic.

Acknowledgements: All JC2RT on-site investigators and the Joint Trauma System (JTS), and AFMS for funding support.


Appendix F: Abstract Template for Symposium

TITLE: A R I A L F O N T , 1 4 POINT-SIZE, CENTERED, BOLDFACE, ALL CAPS, TWO SPACES BETWEEN

WORDS

Presenter(s) and Moderator and Their Background: Use Arial font, 12 point-size, centered, and boldfaced with presenter(s) name first. Provide first and last

names, credentials, department, college/school (if applicable), and institutional affiliations (e.g., Jill Hernandez, Ph.D., Biomedical Engineering, College of

Sciences, The University of Texas at San Antonio). If presenter or moderator is in the military, place military rank abbreviation in front of the first name (see chart

on pages 15-17). Include 2-5 sentences that discuss each presenter’s background related to the theme or topic.

Abstract Body: Arial font, 12-point size, use 1” page margins on all sides. Indent the first line of each paragraph by half an inch. Use both left and right justification. Superscripts and subscripts should be 9-point size. In no more than 1,000 words the symposium description should include as many of the following as are applicable, preferably in this order: (1) objectives of the session; (2) significance of the theme or topic; (3) a discussion of the research or perspective that each presenter will contribute; (4) a discussion of how the session will be structured, including the and strategies to facilitate audience discussion.

Please inc lude acknowledgements and funding support . You are required to use this template for your abstract submission.


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