Abst rac t s 173

C-9.1 #229

A COMPUTER QUALITY CONTROL ANALYSIS OF SEROLOGICAL TRAY REACTIONS BY GROUP AND INDIVIDUAL TECHNOLOGISTS. Edgar Cooper, Dod Stewart, Ochsner HLA Laboratory, New Orleans, LA.

We have developed a program which can be employed to compare the tissue typing and tray reading proficiency of individual technologists. The first step is to perform an analysis on a particular lot of typing trays and assign a cumulative r-value. This analysis will provide an overview of the performance of the tray for all technologists. The trays can then be sorted into additional individual files by technologist. These files can then be analyzed and compared to the overall performance for the tray.

This program can be used to detect patterns of over-scoring or under-scoring among various technologists. On a recent lot of 265 local trays run by six technologists, the r-value ranged from 0.78 to 0.94 for a tray with a cumulative r-value of 0.86. Over a significant number of trays, this would imply that the quality of the trays were more dependent on the technologist than the quality of the sera on the trays.

The program is now run in a dbase environment but is in the process of being convened to the C programming language. This will enable the program to be run on any IBM compatible machine. The program will use the ASCII file formats created by the Robbins Autoscope support program: SCOPESCR. In addition, an ASCII file consisting of a trayid, tech id and scores in a predefined tbrmat can also be used (when the Robbins Autoscope program is not used). The individual and group file is compatible with the Robbins TRAYCHECK program but can be easily convened for export into another analysis program.

TECH NUM TRAYS TP FP FN TN R-VALUE ODRI-12 265 5407 937 234 12445 0.86

1-ODR1-12 67 1317 300 69 3135 0.83 2-ODRl-12 58 1231 96 17 2832 0.94 3 - ODR 1 - 12 48 904 321 27 2204 0.78 4-ODRl-12 30 597 156 25 1364 0.84 5-ODR1-12 28 601 82 45 1284 0.86 6- ODR 1 - 12 24 504 129 16 1079 0.82

C-9.1 #230

A COMPARISON BETWEEN MLC AND PCR-BASED MOLECULAR TECHNIQUES (FINGERPRINTING AND CROSS-MATCHING) IN BONE MARROW TRANSPLANT PATIENTS. CM Spier, RJ Williams, DK Eklund, P Schubart, and PR Finley, Department of Pathology, University of Arizona, Tucson, AZ.

This study compares results from standard MIX2 study and pcr-based fingerprinting and cross- matching in 7 bone marrow transplant recipients. Four of the 7 were Class II identical to their donors; 1 had insufficient B cells for serologic typing and 4 had insufficient cells for MLC. Diagnoses varied: 6 had leukemia or lymphoma, 1 aplastic anemia. MLC in 2 of 3 patients studied suggested donor ceils would be compatible. Molecular testing for the second exon of the DRB gene and key sequences of the DPB allele was performed using standard per based technique and formation of hetero-duplex DNA hybrids between recipient and donor. Two sets of normal HLA-DR serologically identical volunteers (nos 8 and 9) were also included. Results: patient Dx MLC (% RR) Molecular Study Follow-UP

(DR) DRB DPB XM(DRB) 1. ID ALL RvD* 0.7/DvR* 1.4 ID ND ID BMT - >CR 2. ND ML ND ID ID ID BMT pending 3. ID CML RvD* 22/DvR* 13 ID NID ID Fatal GVHD 4. NID AML ND NID ID NID BMT -> CR 5. ID ALL ND ID ND ID DOD pre BMT 6. ND ALL ND ID NID ID DODpreBMT 7. ID ApArt RvD* 0.4/DvR* 0.2 ID ND ID BMT -> CR 8. ID Vntr AvB* 3.6/BvA* 14.9 ID NID ID A&W 9. ID Vmr AvB* 107/BvA* 69 NID NID NID A&W

[ID, identical; NID, not identical, ND, not done] These preliminary results are promising. The advantages of these pcr-hased techniques are

demonstrated, especially when MLC or Class II serologic typing is not possible. Results of MLC shows its value as an indicator of all of the Class II reactivities as confirmed by the molecular studies. Further study on more patients is required and is currently underway.