a blood samples were allowed to clot at r.t for 1 hour, and centrifuged to get sera stanford blood...
TRANSCRIPT
A
Blood samples were allowed to clot at R.T for 1
hour, and centrifuged to
get sera
Stanford Blood
CA State Blood
Stanford Serum Samples
CA State Serum Samples
Kept @ R.T for 7 days
Immediately freeze
freeze
Stanford Group A Samples
CA StateSamples
Stanford Group B Samples
freeze
Kept @ R.T for variable length of time up to 7
days
Figure 1
Transit
B
Pool 5 individual urine samples
Split into 6 identical samples
1
2
3
4
5
6
Day 1
Process
Day 2
ProcessR.T.
Day 3 Day 4 Day 5
ProcessR.T.
ProcessR.T.
ProcessR.T.
ReceiveFEDEX
Process
5 X
Figure 1
ANOVA P value
Den
sity
Total # of feature: 562
0.0 0.2 0.4 0.6 0.8 1.0
0.0
10
20
30
Figure 2
A
Figure 2
A
ANOVA P value
Den
sity
Total # of feature: 100737
0.0 0.2 0.4 0.6 0.8 1.0
0.0
0.5
1.0
1.5
Figure 2
B
Stanford Group B vs. A Stanford Group A vs. CA State
Den
sity
0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0
Tukey HSD P value between sub groups
Stanford Group B vs. CA State
0
2
4
6
8
10
Figure 3
A
Day 2 vs. Day 1 Day 3 vs. Day 1 Day 4 vs. Day 1 Day 5 vs. Day 1 FEDEX vs. Day 1
Den
sity
0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0
Tukey HSD P value between sub groups
0
2
4
6
8
10
12
Figure 3
B
376112
Stanford Group B vs. A CA state vs. Stanford Group A
117
-3 < t < 3t < -3 t > 3
161284
-3 < t < 3t < -3 t > 3
74M
W/1
0000
02
46
Figure 4
A
15516728
t > 3
Day 2 vs. 1
-3 < t < 3
t < -3
Day 3 vs. 1
-3 < t < 3
33
t > 3t < -3
553
Day 4 vs. 1
-3 < t < 3
38
t > 3t < -3
16325 1469
Day 5 vs. 1
-3 < t < 3
149
t < -3
15298
t > 3
761
FEDEX vs. day 1
-3 < t < 3
173
t < -3
15982
t > 3
12 16565 339
MW
/100
01
3
Figure 4
B
Stanford Group A
StanfordGroup B
CA State
Distribution of CV of each feature
0.0
-1.0
2.0
1.0
-2.0
Distribution of DCV of each feature
Stanford GroupA – CA State
Stanford B– CA State
Stanford GroupB – A
1.0
0.5
2.0
1.5
0.0
Figure 5
A
Distribution of CV of each feature Distribution of DCV of each feature
1.0
0.5
2.0
1.5
0.0
Day 1 Day 2 Day 3 Day 4 Day 5 FEDEX Day 2- Day 1
Day 3- Day 1
Day 4- Day 1
Day 5- Day 1
FEDEX- Day 1
0.0
-1.0
2.0
1.0
-2.0
Figure 5
B
Stanford Group A
StanfordGroup BCA State
100
200
300
400
500
600
000
Stanford Group A
StanfordGroup BCA State
100
200
300
400
500
600
000
ALB – MW 66224
CO3 – MW 8125
APOC1 – MW 6629
TF – MW 78284
Figure 6
1. THP 982.59 VLNLGPITR2. THP 1047.48 SGSVIDQSRV3. THP 1211.66 DQSRVLNLGPI
4. THP 1225.69 SRVLNLGPITR5. THP 1324.76 IDQSRVLNLGPI6. THP 1423.83 VIDQSRVLNLGPI 7. THP 1468.82 DQSRVLNLGPITR8. THP 1510.87 SVIDQSRVLNLGPI9. THP 1567.91 GSVIDQSRVLNLGPI10. THP 1581.91 IDQSRVLNLGPITR11. THP 1654.91 SGSVIDQSRVLNLGPI12. THP 1680.98 VIDQSRVLNLGPITR13. THP 1755.96 SGSVIDQSRVLNLGPIT14. THP 1768.01 SVIDQSRVLNLGPITR15. THP 1912.07 SGSVIDQSRVLNLGPITR16. THP 2040.16 SGSVIDQSRVLNLGPITRK
Figure 7
A
Sig
nal
/100
000
20
4
THP 1680.98 THP 1912.07THP 1423.83
Figure 7
B
Stanford Group B
StanfordGroup A
CA State
Mo
lecu
lar
Wei
gh
t
MIN
MAX
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
Figure 8
A B
A VS BBigger Smaller
Figure 9
A B
A VS CBigger Smaller
Figure 10
A B
B VS CBigger Smaller
Figure 11
A B
Day 2 VS 1Bigger Smaller
Figure 12
A B
Day 3 VS 1Bigger Smaller
Figure 13
A B
Day 4 VS 1Bigger Smaller
Figure 14
A B
Day 5 VS 1Bigger Smaller
Figure 15
A B
Fedex VS Day 1Bigger Smaller