94vg
DESCRIPTION
94PCRG##.13. PCR gel template: Fill in the #s and Xs with the appropriate information. 94VG. 94VK. 94MK. 94AS. 94PV. 94KR. 94SM. 1. M. 13. 1. 1. 1. 1. 1. 4000. 3000. 2000. 1650. 1000. 850. 650. 500. 400. 300. 200. 100. Insert size. 80. 800. 280. 210. 450. 300. - PowerPoint PPT PresentationTRANSCRIPT
94VG
175 300Insert size
M
94PCRG##.13
450 210 280 80 800
13 1 1 1 1 1 1
PCR gel template: Fill in the #s and Xs with the appropriate information
650
10001650
2000
4000
500400300
850
200100
3000
94AS94MK94PV94KR94SM 94VK
94RS
320 550Insert size
94PCRG##.13
570 700 750 680 500
M 1 1 1 1 1 1
PCR gel template: Fill in the #s and Xs with the appropriate information
650
10001650
2000
4000
500400300
850
200100
3000
94PS94RB94PP94SP 94AK94SN13
1.Delete the gel picture in this slide.
2.Paste/import cropped gel here and resize to fit markers (note:
some markers and lanes may need to be moved to fit the gel
image).
3.Label lanes with the proper clone names and add student
names (e.G. Change #XX to 20WS and the # to your clone
number 1, 2, or 3).
4.Indicate the calculated size of the insert by pcr at the bottom of
the gel (e.G. ### To 500 bp). Note: remember to subtract the
vector length 200 bp.
5.Save file as a .Jpeg with the proper pcrg name i.E. 20pcrg1.11
6.Upload file to your school’s PCRG album on picasa.
7.Link the image of the file to your clones on the google docs
clone report sheet