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DescriptionThe Atlas Human Hematology/Immunology Arrayincludes 406 human cDNAs, nine housekeepingcontrol cDNAs, and negative controls immobilized induplicate dots on a nylon membrane. Two identica

arrays are included, along with reagents or synthesizingradioactively labeled cDNA probes. A transparenOrientation Grid is also provided to allow identifcationo cDNA's corresponding to the positive hybridizationsignals. See the enclosed CD-ROM or unctionaclasses and complete gene representation on thisarray.

package contents

Bx 1:2 Atlas Human Hematology/Immunology

(10 ng o cDNA/dot)12 l Hematology/Immunology CDS Primer Mix

1 Blank Nylon Membrane(s)1 Orientation Grid(s) (PT3140-2)

Bx 2:120 l 10X dNTP Mix (or dATP label)

(5 mM each dCTP, dGTP, dTTP)600 l 5X Reaction Buer15 l MMLV Reverse Transcriptase

(100 units/ l)120 l DTT (100 mM)60 l 10X Termination Mix

2.5 g Human Placenta Control Poly A+ RNA(0.5 g/l)

65 l Human C0t-1 DNA

1.2 ml Deionized H2O

Bx 3:100 ml ExpressHyb Hybridization Solution

1 Atlas NucleoSpin Extraction Kit

otherUser Manual (PT3140-1)CD-ROM (PT3593-CD)

proDUct: alhum hmly/immuly ay

cataLog n.634507

Lot nUMBer

Specifed on product label

storage conDitionsStore Atlas Arrays at 20C sealedin a plastic bag away rom light.Store Express Hyb HybridizationSolution and Atlas NucleoSpinExtraction Kit at room temperature.Beore using hybridization solution,dissolve any precipitate with gentlewarming.Store Control Poly A+ RNA at

70C

Store all other reagents at 20C

sheLF LiFe1 year rom date o receipt under properstorage conditions.

shipping conDitionsBoxes 1 & 2: Blue ice (4C) or Dry ice

(70C)Box 3: Room temperature

APPROVED BY: _________________________

(PA2Y194)

For research Use onLY

QUaLitY controL Data

See back o page.

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QUaLitY controL Data

1. The size and identity o all cDNAs were verifed by PCR amplifcation, agarose/EtBr gel electrophoresis andsequencing.

2. The Control Poly A+ RNA was electrophoresed on a ormaldehyde/agarose EtBr gel. The pattern observed was thatexpected or human placenta poly A+ RNA.

3. A biotinylated cDNA probe was synthesized using the control poly A+ RNA according to the Atlas SpotLight LabelingKit (Cat No. 634803) User Manual or a radioactively labeled probe was synthesized using the Control Poly A+ RNAaccording to the Atlas Array User Manual. This probe was hybridized to the array, and the expected hybridizationpattern was observed.

Atlas Human Hematology/Immunology Array Cat No. 634507

PA2Y194 www.clontech.com Clontech Laboratories, Inc

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Clontech products are to be used or research purposes only. They may not to be used or any other purpose, including, but not limited to, use in drugsin vitrodiagnostic purposes, therapeutics, or in humans. Clontech products may not be transerred to third parties, resold, modifed or resale, or used to

manuacture commercial products or to provide a service to third parties without written approval o Clontech Laboratories, Inc.

The Atlas Array products sold by Clontech are or research purposes only. Certain DNA sequences included on the Atlas Arrays may be covered byU.S. patents. Presently, it is not clear under U.S. laws whether commercial users must obtain licenses rom the owners o the rights to these U.S. patentsbeore using Atlas Arrays.

These products and the sequences o the polynucleotides thereon are intended to be used or the purchasers own internal research purposes only andmay not be used or drug diagnostic purposes or human use.

DISCLAIMER OF RIGHTS

No license is conveyed with the purchase o this product under U.S. Patents Nos. 5,804,375, 5,994,056 and 6,171,785, andcorresponding patent claims outside the U.S., relating to the 5 nuclease and dsDNA-binding dye processes. Further inormation on purchasing licensesmay be obtained by contacting the Director o Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, Caliornia 94404, USA.

Clontech, the Clontech logo and all other trademarks are the property o Clontech Laboratories, Inc.Clontech is a Takara Bio Company. 2006