1

T ECHNI CAL DATA S HEET OF DI ARS T OP

APPLI CAT I ON I N PRODUCT I VE BREEDI NG FACI LI T I ES I NT EGRAL ROAS T ED CEREAL COMPLEMENT ARY FEED

FOR ALL ANIMAL SPECIES. E U R OF E E D T E CH NOL OGI E S s .r .l . thanks to extens ive exper ience and exper iments in l ives tock farms , have developed a natural vegetable product able to improve animals ’ yield, by a cor rect “modulation” in the development of microorganism which are present in the diges tive apparatus thanks to the DI AR S T OP vegetable components , well dosed among themselves and elaborated by innovative technological sys tems , are able to improve the diges tive apparatus s tate of health, somtimes in danger because of the alteration of the microbial intes tinal flora typical in intens ive breeding. DI AR S T OP acts with a phys ical protection creating a fi lm on the gas trointes tinal walls and in this way PREVENT S GAS T RO-ENT HERI C DI S EAS ES (necrotic enther itis , gas tro-enther itis , aspecific diar rohea and dehydration etc.). I t has a deconges ting action on mucosae and I MPROVES T HE ABS ORBT I ON of NUT RI ENT S and ENHANCES T HE EFFECT I VENES S OF T HERAPI ES AGAI NS T GAS T RO-ENT HERI C DI S EAS ES . DI AR S T OP helps improving the health of animals that are s tres sed by diets with high protein content or bred in inadequate facilities or technologies . I t regular izes the speed of the trans it in the “chimo”, keeps intes tines clean, improves the absorbtion of nutr ients , reduces abdominal swelling and flatulences , faeces become more compact. I ts aroma improves appetite. I t can be used: From the breeders as feed additive, in par ticular for lactating animals . As suppor t from integrator is ti, as a substitute of aux inic antibiotics and/or chemioprofilax ic for animals that have enther ic diseases . Added to indus tr ial feed to prevent a large par t of enther ic diseases , improving growth and yields . After several tr ials that have been car r ied out in farms there has been a reduction of diar rohea and a better growth in compar ison to non treated animals and animals treated with aux inic products .

2

COMP ONE NT S

• I ntegral roas ted barely meal integral roas ted oat meal I ntegral roas ted r ice meal I ntegral roas ted wheat meal

ANAL YS I S

Ar senico 0,019 mg/Kg Cadmium 0,012 mg/Kg Mercure < LQ mg/Kg Lead 0,041 mg/Kg Water 1,1% t.q. T otal protein 9,3% t.q. T otal fats 2,2% t.q. Crude fibre 11,8% t.q. Ashes 4,8% t.q. S tarch 17,5% t.q. Copper as Cu 3,9 ppm t.q. Z inc as Z n 21,7 ppm t.q. I ron as Fe 29,9 ppm t.q. T otal Phosphorus as P 0,5% t.q. S ulphates 430 ppm t.q. Chlor ides 895,4 ppm t.q. Calcium as Ca 571 ppm t.q. Magnes ium as Mg 0,16% t.q. Potas s ium as K 0,62% t.q. Fructose 0,30% t.q. Maltose 1,20% t.q. I nsetticidi Organo Clorurati absent I nsetticidi Organo Fos forati absent I nsetticidi Carbammati Gruppo 1 absent Aflatoxins Determination inHPLC Aflatoxin B1 absent Aflatoxin B2 absent Aflatoxin G1 absent Aflatoxin G2 absent

3

DOS AGE S : W H I T E ME AT CAL VE S From the 2nd to the 30th day 10 g/animal/meal. From the 31st day to the s laughter ing from 10 to 20 g/animal/meal. I n case of enter itis 50-100 g/animal/meal ti l l the problem is solved. N.B . D I AR S T OP improves the quality and the appearance of meat and the iron it contains , s ince it by-passes , does not darken its color , but makes the meat whiter .I n order to reach the r ight weight in advance, dur ing the las t per iod increase by 8-12% the dosage of milk and of DI AR S T OP to 20-25 g/animal/meal.I n cas e of dys ent er y w e w ould r ecommend t o r educe t he dos age of milk by 15-20% for 3-4 meals and give to the animal the same quantity of liquid.

CAL VE S I N S T AB L E S Jus t after colos trum 20 g/meal the fir s t week and then reduce the dosage to 10 g/meal. N.B . T he fur will become br ighter .

MI L K I NG COW S 100 g/day dur ing the fir s t week, as soon as faeces become homogeneous reduce the dosage to 50 gr . 100/150 g / meal in case of ser ious dysentery, as soon as the problem is solved, return to the usual dosages .

N.B . I f DI AR S T OP is dosed continuous ly in the unifeed car r iage at 10-20 g/meal it reduce by 50% the cases of rumen movement.

P I GS Dosage: from 0,5 % to 2% in the feed up to 35 Kg, at 1% in the feed for weaning and at 0,5 % in the feed for finishing.

T U R K E YS Dosage: from 0,5% to 2% for the fir s t per iod feeds , at 1% dur ing the second per iod and at 0,5 % dur ing the final per iod.

E GG L AYE R S Dosage: from 0,3% to 0,5% for the fir s t 3-4 months , then increase to at 1% dur ing las t months of the cycle. R AB B I T S D I AR S T OP is used at dosages that may vary from 1% to 2,5% in accordance with the results we want to obtain.

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GOAT S Dosage: from 1% to 2% in the feed for the fir s t 25 days , from 0,5% to 1% dur ing the final phase.

DURI NG T HE PROCES S I NG OF PELLET I Z ED FEED − I ncreases the shelf life and the efficacy of the pelletiz ing machine. − Eliminates the res idues of powder of the pellet. − I ncreases the hardness of the pellet.

5

PI GS T OP

L is t of tes ts Compar ison with tes ter s . Compar ison among 4 kinds of integrator s . Feed mixtures differently adde

T ES T N.01

T it le :

A tr ial was car r ied out on pigs in order to s tudy the effects of P I GS T OP , when added to the feed mixture.

Mater ials and methods :

I n the tr ial 120 pigs were divided into 2 groups of 60 subjects each uniform in sex, age, weight and nes t. A group, the control, was given a commercial feed mixture, whereas the other group received the same feed mixture with the addition of 3 0 0 gr . P I GS T OP /q feed mixture.T he tr ial s tar ted at weaning (about 29 days of age ) and ended after 33 days when the pigs were about 59-64 days old.

R es ult s : 3 0 0 gr . P igs t op X 1 0 0 K g f eed CONT ROL

Nr . Animals 60 60

Days On T r ial gg 33 33

I nitial Weight kg 6.01 6.04

F inal Weight kg 16.70 16.58

Daily Gain 325 310

Feed Convers ion Efficiency 1.89 2.02

(Kg Feed/Kg Gain) gr 615 646

Daily Feed I ntake 52% 48%

Mor tality 5% 5%

6

T ES T N.02

T i t le :

T r ials were car r ied out on 4 groups of pigs fed with a feed mixture having 4 different additions .

Mater ials and methods :

I n the tr ial 80 pigs were util ized, they were divided into 4 groups uniform in sex, age and weight.

T he groups received the same feed mixture but containing 4 different additions . (tab.1).

T he tr ial s tar ted at the wear ing when the pigs were about 28 days old and ended after 41 days when the subject were about 70 days old.

R es ult s : T ABLE 1 : Addition of the 4 examined feed mixtures

F eed mixt ur e w it h miner al vi t aminic addit ion

Feed mixture with mineral vitaminic addition + 300 gr . of P I GS T OP

Feed mixture with mineral vitaminic addition + 80 gr . of CARBADOX 10x100Kg. + 40 ppm of AVOPARCI NA

Feed mixture with mineral vitaminic addition + 80 gr . of di CARBADOX 10 x 100 Kg. + a product containing yeas t and lactobacill i.

7

T AB L E 2

Feed Mixture 1 2 3 4

Nr . Animals 20 20 20 20

Days On T r ial 41 41 41 41

I nitial Weight 5.84 5.77 5.86 5.69

F inal Weight 19.01 20.62 20.31 20.06

Daily Gain (Gr) 321 374 352 350

Feed Convers ion Efficiency 2.04 1.88 1.97 2.00

Daily Feed I ntake 655 680 695 700

Feed Mixture Efficiency 49% 53.2% 50.7% 50%

Mor tality 5% 0% 0% 5%

T ES T N. 03

T itle :

T r ial results car r ied out on pigs fed with feed mixture differently added.

Mater ials and methods :

I n the tr ial 140 pigs were util ized and they were divided into 2 groups uniform in sex, age and weight. T he groups on tr ial received feed mixture with 2 different additives .

T he tr ial s tar ted at the 65th days of age and las ted 35 days .

8

R es ult s :

T ABLE N.01 - Addition to the 2 feed mixtures .

A feed mixture with mineral vitaminic addition +

T YLAD 40 100 grx100Feed

FURANI CO 100 100 grx100Feed

CARBADOX 10 80 grx100Feed

B Feed mixture with mineral vitaminic addition +

P I GS T OP 300 grx100Feed

T YLAD 40 50 grx100Feed

CARBADOX 10 50 gr x100Feed

T ABLE N. 02

Feed Mixture 1 2

Nr . Animals 70 70

Days On T r ial gg 35 35

I nitial Weight kg 18.90 18.81

F inal Weight kg 41.30 43.15

Daily Gain gr 640 695

Feed Convers ion Efficiency 2.61 2.40

Feed Mixture Efficiency 38.21 1.60%

9

T S T OP

T itle :

T he use of T S T OP in the feeding of turkeys .

Mat er ials and met hods :5

T he tr ial las ted 50 days when the subject were about 100 days old. I n the tr ial 120 animals were util ized, they were divided into 2 groups of 60 subject each. T he turkeys were weighed at the beginning of the tr ial and at the end at about 150 days of age. Both the turkeys of group 1 (exper imental thes is ) and the turkeys of group 2 (control) were given two kinds of feed mixture. T he only difference between the 2 groups was the supplementary addition of 300 g/100 Kg of T S T OP . I n table 1 there are the chemical analyses of the 2 feed mixtures given, type A from 100th to 125th day of age and type B from the 120th day to the s laughter day. T AB. NR.1

T YPE A T YPE B

Mois ture 13.00 13.00

Crude Protein 21.00 18.50

Ether Extract 7.50 9.50

Crude Fibre 3.00 3.00

Ash 6.30 6.00

Cys tine 0.20 0.20

Methionine 0.35 0.30

Lys ine 0.80 0.60

I n table 2 there are the additions per Kg to the feed mixture (ty-pe A and type B)

10

T AB. NR.2

T YPE A T YPE B

Vitam. A 14.000 U.I Vitam. A 10.000 U.I .

Vitam. D3 4.500 U.I . Vitam. D3 2.500 U.I .

Vitam. E 30 mg Vitam. E 20 mg

Vitam. B1 4 mg Vitam. B1 2 mg

Vitam. B2 6 mg Vitam. B2 4 mg

Vitam. B6 5 mg Vitam. B6 3 mg

Vitam. B12 0.02 mg Vitam. B12 0.02 mg

Vitam. K3 5 mg Vitam. K3 3 mg

Vitam. Pp 75 mg Vitam. Pp 50 mg

Vitam. H 0.2 mg Vitam. H 0.2 mg

Cholin 750 mg Cholin 750 mg

D. Pantothenic Ac 15 mg D. Pantothenic Ac. 15 mg

Fol ic Acid 1 mg Fol ic Acid 1 mg

Cobalt 0.5 mg Cobalt 0.5 mg

I ron 50 mg I ron 50 mg

I odine 1 mg I odine 1 mg

Manganese 50 mg Manganese 50 mg

Copper 5 mg Copper 5 mg

Z inc 100 mg Z inc 100 mg

S elenium 0.050 mg S elenium 0.050 mg

11

Results : T ABLE N.03

3 0 0 gr T S T OP x 1 0 0 K g F E E D CONT ROL

Nr . Animals 60 60

Days On T r ial gg 50 50

I nitial Weight kg 8.05 8.16

Final Weight kg 14.90 14.60

T otal Gain 6.85 6.44

Daily Gain gr 137 129

Feed Convers ion

Efficiency 3.24 3.46

Feed I ntake 22.19 22.28

Yield 30.8% 28.9%

Mortality 6.6% 8%

Mois ture Litter 30% 35%

12

T DAI RY

T ES T 01

T I T LE

DES CRI PT I ON

RES ULT S

T ABLE

CONCLUS I ON

T ES T NR. 01

T itle:

Repor t and results from the use of T DAI R Y in the daily feed por tion to milking cows in a dairy farm in S ores ina (Cremona)

Descr iption of the tes t:

I n the farm are bred animals of the same species , on the average weight, age and or igin are the same. We car r ied out a tes t adding T DAI R Y in the following per iods and at the following dosages :

PERI OD QUANT I T Y

01/02/02 – 28/02/02 25 gr / cow / day

11/03/02 – 18/03/02 50 gr / cow / day

18/03/02 - … 25 gr / cow / day

13

Results :

I n the following table are the analyses done by the Z ooprophylactic I ns titute on the milk produced by the cows dur ing the treatment:

Dat e S omatic cells /ml Geom. average bacter ial count Geom. average cells

07/02/2002 422.000 89.000 422.000

18/02/2002 367.000 76.000 437.000

11/03/2002 545.000 81.000 453.000

18/03/2002 296.000 61.000 405.000

03/04/2002 332.000 61.000 383.000

16/04/2002 263.000 52.000 358.000

As you can see from the table the analyses done on March, 11 2002 the somatic cells of the milk have increased. T his was due to the fact that cut maize had finished in one container and it was poor quality fodder . We increased the quantity of T DAI R Y and gave to the animals 50 gr /animal. As you can see from the second analys is of the month the parameter s have then returned back to the usual amount. T he s light increase of somatic cells on Apr il, 4 was due to the opening of a new container of cut maize, and again the fodder was not of good quality. From that date on the dosage was reduced again to 25 gr/animal.

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Moreover the milk production increased as we can observe in the following table.

Conclus ion :

T he use of T DAI R Y at the above dosages greatly reduced somatic cells in the milk.

I n general us ing this product there have been evident benefits s ince the animals can diges t the fodder better and the faeces are more cons is tent.

M IL K P R O D U C T IO N

0

2 0

4 0

6 0

D A T E

QU

AN

TITY

OF

MIL

K (1

00

lt)

M O R N IN GE V E N IN G

M O R N IN G 4 8 ,2 8 4 9 ,8 2 5 0 ,4 2 5 1 ,6 8 5 2 ,5 5 5 0 ,5

E V E N IN G 4 2 ,3 4 4 5 ,2 4 4 9 ,6 4 5 ,0 8 4 5 ,0 8 4 4 ,7 6

0 7 /0 2 / 1 8 /0 2 / 1 1 /0 3 / 1 8 /0 3 / 0 3 /0 4 / 1 6 /0 4 /

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RABS T OP

L I S T OF T ES T S

T r ial car r ied out in order to s tudy the effects of R AB S T OP , when added to the feed mixture, on rabbits .

T he use of a cereals additive “ R abs t op ” in the fattening of rabbits

T ES T N. 01

T itle :

T r ial car r ied out in order to s tudy the effects of R AB S T OP , when added to the feed mixture, on rabbits .

Mater ials and methods :

I n the tr ials 136 rabbits were utilized, they were divided into two groups of 68 subjects each uniform in sex, age weight and nes t .

A group, the control, was given a feed mixture without any additives , whereas the other group received the same mixture where R AB S T OP was added (300 gr R AB S T OP /kg feed). T he tr ial s tar ted at the weaning (about 28 days of age) and ended after 43 days when the subject were s laughtered.

16

R es ult s :

R AB S T OP 3 0 0 gr / 1 0 0 K g. f eed CONT ROL

Nr . animals 68 68

Days on tr ial gg 43 43

I nitial weight kg 665 667

Final weight kg 2.294 2.218

Daily gain gr 37,8 36,1

Feed convers ion efficiency 3,09 3,38

(Kg feed/Kg gain) gr 117 122

Daily feed intake 32% 29,5%

Mor tality 4,41% 5,88%

T ES T N. 02

T i t le :

T he use of a cereals additive “R AB S T OP ” in the fatt ening of rabbits , (this research was car r ied out by prof. Manfredo Manfredini c/o the Rationing Provis ions I ns titute, Markets and indus tr ies of animal or igin food , Univer s ity of Bologna, Faculty of Medicine and Veter inary S cience).

17

Mater ials and methods :

I n the tr ials 144 hybr id rabbits were uti lized, they were divided into 3 groups of 48 subjects each uniform in sex, age, weight and nes t.

A group, the control, was given a feed mixture, whereas the other two groups , A1 and A2, received the same mixture as the control but with the addition of 250 and 300 g R AB S T OP / 100 Kg. feed respectively.

T he tr ial, which las ted 68 days , s tar ted at the weaning of animals (about 80 days of age) and ended at the s laughter . T he rabbits were weighed individually at intervals of two weeks , then they were weighed for the las t time on the day of the s laughter .

At the same intervals feed intakes and respective feed conver s ion indices were recorded.

At the s laughter (at a weight of approx imately 2.5 Kg. after fas ting for 8 hours ) live weight and weight of the chil led carcass were measured. Refr igeration took place in a refr igeration cell at a temperature of 4° C for 2 hours .

R es ult s :

T he feed intakes did not differ s tatis tically in the three groups ; however the feed intakes tended to reduce with the addition of R AB S T OP ; the smalles t inges tion seemed to be influenced by the level of addition (106,9 VS 108,9 VS 111,5 g/d respectively in A2, A1, and C).

T his tendency is more clear in table 4 where the feed intakes of rabbits fed with added feed mixture tended to reduce from the fir s t days of the pos t- wear ing.

T he feed convers ion indice decreased on account of the weight gains which are s imilar in the 2 groups .

I n table 4 the tendency of the feed conver s ion in the different phases is also showed.

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Conclus ions :

T he results obtained showed that the use of R AB S T OP pos itively affected the per formances of fattening rabbits and the feed intakes and conver s ions .

Par ticulary, the rabbits fed with “R AB S T OP ” ( in the dosage 100 gr /100 kg feed mis ture) showed a tendency to reduce feed intakes and to l imit the feed conver s ion indice. T he mor tality rate was also reduced in the groups receiving the addition .

All these results showed that the product on tr ial pos itively affected the per formances of fattening rabbits .

GR OU P S (1) (1)

P AR AME T E R S CONT R OL A 1 A 2

Mois ture % 5,40 5,30 5,30

Crude Protein % S .S . 17,68 17,50 17,37

Ether Extract “ 3,08 3,22 3,16

Crude Fibre “ 18,66 18,57 18,74

Ash “ 8,82 8,95 9,02

Nitrogen Free Extract “ 51,76 51,76 51,71

Calcium “ 1,12 1,15 1,10

Phosphorus “ 0,37 0,37 0,37

(1) R AB S T OP has got this chemical compos ition : Crude protein 10,67 ; Ether extract 3,24 ; Crude fibre 9,46% ; Ash 3,09% ; Nitrogen- free extract 73,54 ; NDF 18,08 ; ADF 14,64 ; Calcium 0,03 ; Phosphorus 0,05 ; pH 5,60.

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T AB. 2 - P R ODU CT I VE P E R F OR MANCE S

P AR AME T E R S Cont r ol A 1 A 2 Var iance S ignif icancy

Nr . Animals 43 45 47 - -

I nitial Weight Kg 0,65 0,65 0,65 0,01 N.S .

F inal Weight Kg 2,52 2,48 2,49 0,03 N.S .

Daily Gain g 27,5 26,9 27,1 5,52 N.S .

Daily Feed I ntake g 111,5 108,9 106,9 5,28 N.S .

Feed Conver s ion Efficiency

4,06 4,05 3,94 0,004 N.S .

T AB. 3 - W E I GH T GAI NS I N T H E D I F F E R E NT P E R I ODS ( gr / d)

P E R I OD Cont r ol A 1 A 2 Var iance S ignif icancy

0 : 14 D 29,5 29,3 29,0 22,2 N.S .

15 : 28 D 31,0 30,5 29,3 30,3 N.S .

29 : 42 D 30,6 30,4 31,8 16,7 N.S .

43 : 56 D Aa23,1 Bb19,1 Aba21,5 22,5 * *

57 : 68 D 24,8 27,5 25,3 57,3 N.S .

0 : 68 D 26,9 26,9 27,1 5,52 N.S .

* * : P < 0,01

A,B : P < 0,01

a,b : P < 0,05

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T AB. 4 - F E E D I NT AK E AND F E E D E F F I CI E NCY I N T H E D I F F E R E NT P E R I OD

P E R I OD Cont r ol A 1 A 2 Var iance S ignif icancy

0 : 14 D 73,5 71,6 67,6 12,8 N.S .

15 : 28 D 85,9 84,6 84,3 35,6 N.S .

29 : 42 D 126,1 a 120,5b 119,0 b 4,60 N.S .

43 : 56 D 124,8 121,4 124,4 7,70 N.S .

57 : 68 D 166,7 167,3 157,5 36,6 N.S .

0 : 68 D 111,5 108,9 106,9 5,28 N.S .

FEED EFFI CI ENCY

0 : 14 D 2,49 2,25 2,33 0,04 N.S .

15 : 28 D 2,74 2,78 2,90 0,03 N.S .

29 : 42 D 4,13 3,94 3,77 0,04 N.S .

43 : 56 D 5,41 6,37 5,83 0,08 N.S .

57 : 68 D 6,78 6,13 6,22 0,13 N.S .

0 : 68 D 4,06 4,05 3,94 0,04 N.S .

* : P < 0,05

a,b : P < 0,05

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T AB. 5 - S L AU GH T E R DAT A

P AR AME T E R S . Cont r ol A 1 A 2 Var iance S ignificancy

Nr . Animals 43 45 47 - -

WEI GHT At T he S LAUGHT ER

Kg 2,411 2,368 2,383 0,03 N.S .

Weight Los s Dur ing T ranspor t

% 4,35 4,43 4,33 1,57 N.S .

Carcass Weight After Chil ling

Kg 1,473 1,425 1,433 0,02 N.S .

Dres s ing Percentage % % 61,10 60,11 60,16 6,18 N.S .

T AB. 6 - RABBI T S DI ED DURI NG T HE COURS E OF T HE T RI AL

CAGE S CONT R OL A 1 A 2 T OT AL P E R I OD

PERI OD 1 2 3 1 2 3 1 2 3

0-14 0 0 0 0 0 0 0 0 0 0

15-28 0 0 0 0 0 0 0 0 0 0

29-42 2 1 1 1 0 0 0 1 0 6

43-56 0 0 0 0 0 1 0 0 0 1

57-68 0 1 0 0 1 0 0 0 0 2

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CALFS T OP

T E S T S :

1 Compar ing tr ial between two groups of white meat calves .

2 Control of the affection of Calf s t op on the main productive per formances of white meat calves .

T E S T N. 0 1

Compar ing tr ial between two groups of white meat calves .

Mater ials and methods :

T he breeding of 150 heads was made up of subjects of the same breed, quite uniform in weight, age and or igin. T he tr ial was car r ied out on 70 calves which were given CAL F S T OP as follows :

DAYS ON T RI AL QUANT I T Y T OT AL QUANT I T Y

2nd - 30th days 5 gr / head / meal 300 gr

121st - 153rd days 8 gr / head / meal 512 gr

T OT AL 812 gr

T he other 80 calves (the control) received the same feed mixture (kind and quantity of dr ied milk, additions therapy etc.) without any CAL F S T OP .

23

T he tr ial s tar ted when the young calves ar r ived and ended when the calves were s laughtered.

Results :

T AB L E 1

CAL F S T OP ADDE D T O MI L K CONT ROL

nr . Animals 70 80

I nitial weight 54.6 53.8

F inal weight 240.44 235.1

Feed convers ion efficiency 1.60 1.64

T otal feed intake (Kg) 298 298

Milk yield 62.3% 60.8%

Mor tality 2.8% 2.5%

T E S T N. 0 2

Control of the affection of CAL F S T OP on the main productive per formances of white meat calves .

Mat er ials and met hods :

I n the tr ial 220 calves were utilized. T he calves , of the same breed, quite uniform in weight, age and or igin were divided into 2 groups of 116 and 104 subjects respectively.

24

T he tr ial was car r ied out on 116 calves of a group which were given CAL F S T OP as follows :

DAYS ON T RI AL QUANT I T Y T OT AL QUANT I T Y

2nd - 25th days 6 g/head / meal 276 g

26th - 126th days 3 g/head / meal 600 g

127th - 150th days 8 g/head / meal 368 g

T OT AL 1.244 g

T he B group (the control) made up of 104 subjects received the same feed mixture (in quantity of dr ied milk, addition, therapy ) as the A group did but without any CAL F S T OP addition. T he tr ial s tar ted when the young calves ar r ived and ended when the calves were s laughtered.

Results :

CAL F S T OP ADDE D T O MI L K Control

Nr . animals 116 104

I nitial weight 60,3 61,6

Final weight 238,9 234,5

Feed convers ion efficiency 1,61 1,67

T otal feed intake (Kg) 287,52 288,80

Milk yield 61,9% 59,8%

Mor tality 0,8% 1,9%

25

LAYERS T OP

T it le :

Exper imental tr ial on layer s dur ing laying in order to s tudy the effects of L AYE R S T OP when added to the feed mixture ( 300gr .LAYERS x 100Kg feed).

Mater ials and methods :

T he feed was always given ad libitum.

Dur ing the fir s t week feed mixture for pullet was given, then it was gradually replaced by a feed mixture for layer . T he tr ial las ted about 37 weeks as data from 26th to 62nd week of age were recorded.

26

T AB. NR. 1

CH E MI CAL ANAL YS I S I NT E GR AZ I ONE P E R K G

Mois ture 13.00% Vit. A 12.500 U.I .

Crude Protein 17.00% Vit. D3 2.500 U.I .

Ether Extract 4.50% Vit. E 20 mg

Crude Fibre 4.40% Vit. K3 2 mg

Ash 13.00% Vit. B1 3 mg

Cys tine 0.25% Vit. B2 5 mg

Methionine 0.30% Vit. B6 3 mg

Lys ine 0.60% Vit. B12 0.02 mg

Vit. Pp 30 mg

Pantothenic Acid 12 mg

Choline 500 mg

Manganese 90 mg

I ron 40 mg

Z inc 60 mg

Copper 6 mg

Cobalt 0.25 mg

I odine 1.2 mg

Folic Acid 0.5 mg

27

T AB. NR. 2

L AYE R S T OP ADDE D W I T H OU T L AYE R S T OP

Age

Weeks

%

laying

% brok

eggs

egg

weight

layer s

left

feed

intake

%

laying

% brok

eggs

Egg Weight Layers left Feed I ntake

22 29.9 50.8 27.330 29.8 51.2 27.315

23 55.9 52.1 27.265 55.3 52.3 27.245

24 71.7 56.0 27.180 71.4 54.5 27.210

25 81.7 3.5 57.8 27.143 81.5 3.5 56.4 27.173

26 84.4 3.4 58.1 27.096 110 84.1 3.8 57.1 27.151 114

27 84.8 3.4 58.6 27.041 84.6 4.1 57.6 27.112

28 84.2 3.0 59.4 26.989 84.3 4.0 57.8 27.083

29 84.3 3.1 60.5 26.934 84.1 4.1 58.1 27.021

30 87.5 3.0 61.2 26.886 112 86.6 3.9 59.3 26.963 120

31 87.8 3.0 61.6 26.854 86.7 3.8 60.1 26.931

32 87.8 3.2 61.8 26.823 86.4 3.7 60.2 26.893

33 87.4 3.4 62.1 26.775 86.1 3.8 60.5 26.815

34 86.7 3.4 62.8 26.730 110 85.3 3.8 60.8 26.781 116

35 84.7 3.3 62.8 26.670 85.0 3.9 61.3 26.703

36 85.7 3.0 62.9 26.626 85.0 4.0 61.4 26.641

37 85.7 3.0 63.0 26.581 84.8 4.1 60.9 26.603

38 84.6 3.1 62.8 26.541 112 84.2 4.3 61.5 26.554 118

39 84.3 3.4 63.1 26.492 84.1 4.6 61.8 26.512

28

40 83.4 4.0 63.3 26.445 83.6 4.9 62.1 26.451

41 80.0 4.0 63.3 26.387 81.6 5.0 62.0 26.383

42 78.8 4.0 63.5 26.330 108 80.5 5.0 62.2 26.312 116

43 79.5 4.1 63.3 26.295 80.1 5.2 62.4 26.261

44 80.2 4.2 64.1 26.260 80.1 4.9 62.8 26.208

45 79.4 4.3 64.0 26.227 80.3 5.1 62.6 26.143

46 78.9 4.6 64.2 26.193 106 79.6 5.2 62.9 26.093 115

47 75.5 4.2 63.9 26.125 73.6 5.3 63.3 26.002

48 75.3 4.4 64.3 26.101 78.8 5.6 63.2 25.937

49 73.7 4.2 64.5 26.041 74.6 5.7 63.4 25.841

50 74.0 4.1 64.6 25.994 106 74.2 5.7 63.4 25.717 116

51 74.0 4.3 64.6 25.942 74.1 5.8 63.8 25.666

52 74.1 4.4 64.8 25.790 73.0 6.0 63.6 25.593

53 74.0 4.1 65.1 25.773 72.6 5.7 63.5 25.521

54 73.5 4.0 65.0 25.760 107 73.1 6.1 63.9 25.488 114

55 72.2 4.2 64.8 25.741 70.8 5.9 64.0 25.416

56 72.6 4.6 64.9 25.726 69.3 6.1 64.1 25.368

57 73.6 4.6 65.4 25.690 68.8 6.0 64.3 25.294

58 74.2 4.5 66.0 25.653 104 68.6 6.3 64.5 25.231 113

59 73.1 4.2 65.9 25.621 68.8 6.1 64.7 25.118

60 70.2 4.4 66.1 25.593 68.1 6.2 64.6 25.073

61 72.8 4.6 66.4 25.541 67.9 6.8 64.9 25.017

62 70.3 4.3 66.3 25.496 102 66.2 7.4 65.0 24.983 111

29

R es ult s :

T AB L E N.0 3

CAP .1 CAP .2

Laying % 79.3 78.4

B roken Eggs % 3.86 5.04

Egg Weight 63.5 62.2

Mor tality 6.7% 8.53%

Feed I ntake 107.7 115.3

Conclus ions :

T he results obtained (see table 3) showed that the addition of LAYERS T OP

( 300 gr x 100Kg feed) to the feed mixture pos itively affected the per formance of the layer s .We could see how the laying parcentage (79.3% VS 78.4% ) the egg weight (63.5 VS 62.1) was better among the layer s in the shed num-ber 1. T he broken eggs percentage (3.86 VS 5.04) and the daily feed intake ( 107.7 gr VS 115.3 gr ) showed how the addition pos itively affected the per formance of the animals .

We could infer that the sugges ted addition could reduce the production cos t/Kg eggs of around 7-8% .

30

ENZ YMES FOR WHEAT BAS ED FEEDS BROI LER PERFORMANCE T RI AL – T S T OP

A tr ial was conducted with an integrated broiler company to investigate the effects of us ing in broiler feeds .

• T r ial s iz e: 75.000 birds from day old

• T r ial for mat : 6 houses . 3 houses (37.500 birds ) fed s tandard diet (control), 3 houses (37.500 birds ) fed tr ial diet with T B R OI L E R S at the dosage of 3 Kg/T on (treatment)

31

• Feed: The two groups have been fed with the same quantity (3 Kg animal)

OB S E R VAT I ON • T B R OI L E R S treated feed resulted in a 6,90% improvement in Daily L iveweight Gain and in a 4,36% improvement in

Feed Convers ion Ratio. • B irds fed T B R OI L E R S treated feed finished more quickly (one day before), at heavier weights

S L AU GH T E R S L AU GH T E R D A I L Y W H E I GH T

L I V E W E I GH T A GE GA I N F CR

( K g) ( day s ) ( gm / day ) ( k g)

C O N T R O L G R O U PH O U S E 1 1 .7 6 5 4 2 4 2 ,0 2 1 ,7 0H O U S E 2 1 .6 9 0 4 2 4 0 ,2 4 1 ,7 8H O U S E 3 1 .7 8 0 4 1 4 3 ,4 1 1 ,6 9

A V E R A G E 1 .7 4 5 ,0 0 4 1 ,6 7 4 1 ,8 9 1 ,7 2

T R E A T M E N T G R O U PH O U S E 4 1 .8 0 5 4 1 4 4 ,0 2 1 ,6 6H O U S E 5 1 .8 5 1 4 1 4 5 ,1 5 1 ,6 2H O U S E 6 1 .8 0 7 4 0 4 5 ,1 8 1 ,6 6

A V E R A G E 1 .8 2 1 ,0 0 4 0 ,6 7 4 4 ,7 8 1 ,6 5

IM P R O V . O V E R C O N T R O L 7 6 ,0 0 -1 ,0 0 2 ,8 9 0 ,0 7

% IM P R O V E M E N T 4 ,3 6 2 ,4 6 6 ,9 0 4 ,3 6

32

40

41

42

43

44

45

ControlTBROILERS

Daily L ivew eight Gain

33

1

2

ControlTBROILERS

F eed Conver s ion R at io