490series_completemanual

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490 Series

Biological Microscope

OPERATOR’S MANUAL

READ THIS MANUAL BEFORE USING THE MICROSCOPE



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Contents 490 SERIES

Before use ..............................................................................................

1. Nomenclature ....................................................................................

2. Operation ............................................................................................

2-1 Assembly ...........................................................................2-2 Setting up ................... ................... .................... ................

2-3 Focusing .............................................................................

2-4 Adjusting the condenser and diaphragm .............

2-5 Using the trinocular port ................................. ............

2-6 Setting the stage’s stop limit ............................ ..........

2-7 Adjusting focusing tension ................. .................... ....

2-8 Changing the bulb .................... .................... .................

2-9 Changing the fuse .................. .................... ................... .

2-10 Maintenance ....................................................................

3. General specications .................. .................... ................... ...........

4. Conguration drawing ................. .................... ................... ...........

5. Optional Accessories ................... ................... .................... .............

6. Parameters/technical terms ................... .................... ..................

6-1 Objectives ..........................................................................

6-2 Eyepieces ...........................................................................

6-3 Electrical system ................. .................... ................... ......

6-4 Parameters ........................................................................

6-5 Technical Terms ................................... ................... .........

7. Troubleshooting ...............................................................................

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Before use 490 SERIES

IntroductionCongratulations on the purchase of your new AmScope Microscope!

This manual is designed for the B490 and T490 series microscopes.

Though both have identical bases, the B490 is equipped with a

binocular head (2 eyetubes) and the T490 is equipped with a trinocular

head (2 eyetubes & 1 photo/video port). Please take a few minutes to

familiarize yourself with the features and functions of your new

microscope. If you want more information on microscope, parts, and

accessories, please visit our website at:

www.AmScope.com

We recommend that you study this manual thoroughly before operat-

ing the microscope, and that you keep it on hand for future reference.

If you have additional questions or need assistance, please send us an

email at:

[email protected]

Please include the microscope model number in your email so that wecan identify your model and provide immediate help.

Precautions(1) As the microscope is a precision instument, always handle it with

care, avoiding impact or abrupt movement during transportation. Do

not shake the package.

(2) Do not place the microscope in direct sunlight or in high heat. Keep

it indoors in a dry and clean place with temperatures between 32°-100°

F (0°-40°C), maximum relative humidity: 85%

(3) Avoid touching the lenses on the objective and the eyepiece so thatoil and dirt from your ngerprints do not obstruct your view.

(4) Before turning the power on, make sure that the power supply

voltage is consistent with the voltage of your microscope



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1. Nomenclature 490 SERIES

Nosepiece

Objective

Mechanical Stage

Condenser,iris diaphragm

Base lens

Base

Eyepiece

Fig. 1: B490

Diopter

Finefocusing knob

Coarsefocusing knob

Arm

BinocularGemel head

Iris adjustment slider

Filter holder

Slide controls

Power switch

Dimmer

Tension knob

Eyetube base

Head -lock screw



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C-mount

Fig. 2: T490

Adjustabletrinocular port

TrinocularGemel head

Condenseradjustment knob

Limit-stopknob

Coarsefocusing knob

Finefocusing knob

Condenserscrew



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Fig. 3: Bottom

Fig. 4: Condenser, iris and lter holder

FuseLight screw

Halogen

bulb

IrisFilter holder

Condenserscrew Condenser

Iris adjustmentslider

Denitions:

Base Lens Directs the light source towards the slide

Coarse Focusing Knob Used to initially bring the slide into sight and

focus

Adjustable Trinocular Port Threaded port with adjustable length forattachment of digital or video camera



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Condenser & Iris Diaphragm

Controls the amount of light that hits the slide

Condenser Adjustment Knob

Moves the condenser up and down

Condenser Screw Screws the condenser in place, secureing it

to the mechanical stage

Dimmer Controls the amount of light that escapes

from the base lens

Diopter Allows the focus to be perfected for both eyes,

independent of each other

Fine Focusing Knob Used to rene focus to a clear image

Head Lock Screw Screws the microscope head into place

Limit-Stop Knob Limits the upward movement of the mechanical

stage in order to avoid damaging the slide and

objective (adjustable).

Mechanical Stage Mechanically moves the slide along an X and Y

axis for optimal positioning

Nosepiece Houses the objective lenses

Rotating Gemel Head Adjusts eyepieces to t the distance between

the user’s eyes for added comfort

Tension Knob Adjusts the tension of the focusing knobs



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2. Operation 490 SERIES

2-1 Assembly(1) First take the styrofoam container out of the cardboard carton and lay

it on its side, paying attention to which side is labeled up. Remove tape

and open the container carefully so as to avoid dropping and damaging

the optical items. Check carefully to ensure that all parts and accessories

are intact.

(2) Check the parking list below:

One Microscope Body

One Binocular (B490) or Trinocular (T490) Rotating Gemel Head

One Adjustable Trinocular Photo Port (for T490 only)

Four Objectives: 4X, 10X, 40X, and 100X

Two Pairs of Eyepieces: W10X and P16X

Color Filters

One Bottle of Immersion Oil

One 6V/20W Halogen Spare Bulb

One Spare FuseOne Dust Cover

(3) Remove the microscope body from the box and remove the plastic

protective covering. The body of the microscope is composed of the

base, the stage, the arm, and the nosepiece.

(4) Loosen the metal knob located directly above the nosepiece (the

head-lock screw) completely. Place the head, at circular side down, onto

the circular opening at the top of the arm. Make sure the microscope

head is securely on top of the arm and tighten the head-lock screw to

lock the head in place.

(5) For the T490 model only, screw the photo port onto the top of the

microscope head.

(6) Remove the eyetube caps and drop the desired eyepieces into the

eyetubes without touching the lens.



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(7) Screw the objectives into the microscope nosepiece from the lowest

magnication to the highest, being careful not to touch the lens.

(8) Plug in the microscope and turn it on. If no light emerges from the

light source, adjust the dimmer knob on the side of the base.

2-2 Setting up(1) Loosen the head-lock screw and adjust the microscope head so that it

is in a comfortable position for observation. Lock the head-lock screw.

With both eyes open, look into the eyepieces. Adjust the interpupillary

distance by holding the eyetubes and rotating the eyepieces either

towards or away from one another, until one circle of light can be seen.

(2) Place the specimen to be studied on a glass slide (or simply use a

prepared slide), and place it on the stage, holding it sungly in place with

the metal slide-holders of the mechanical stage. Using the slide controls,center the specimen over the stage opening, lining it up with the light

and objective lens.

(3) To adjust the illumination, slowly turn the dimmer until the desired

intensity of light is achieved.

2-3 Focusing(1) Turn the nosepiece to choose an objective. It is easiest to use the

lowest magnication rst (usually 4X) to locate and focus the specimen.

As you move up in magnication, you may have to refocus the image a

little bit each time. When using the 100X objective, a little immersion oil

must be put between the objective and the cover glass on the slide in

order to minimize the distortion cause by air. Always wipe the oil o the

objective with either a glass-cleaning paper or cloth after each use.



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(2) Begin focusing by rst looking with one eye through the eyetube

without the diopter. Close your other eye. Loosen the limit-stop knob

(located on the inside of the coarse-focusing knob) in order to give the

mechanicalstage a full range of movement and adjust the coarse-

focusing knob to bring the slide into focus. You may also need tore-center the specimen on the stage.

(3) When the object is in your eld of vision, adjust the ne-focusing

knob until you see a clear, sharp image. BE ALERT: When moving the

mechanical stage vertically, be careful not to hit the objective lens with

the slide. Remember to use the limit-stop knob to avoid impact between

the objective and the slide when using the 40X and 100X objectives.

Lock the limit-stop knob at the position where the slide almost touches

the objective.

(4) Once the image is focused through the one eyepiece, look through

the other eyepiece and turn the diopter until the image is clear throughthat eyepiece as well

2-4 Adjusting the condenser and diaphragm(1) Using the condenser-adjustment knob, you can change the distance

between the light condenser and the stage. This allows you to control

the concentration of the light hitting your slide.

(2) By Changing the aperture of the iris diaphragm, you can adjust the

background brightness. Adjust the aperture of the iris diaphragmm

using the iris adjustment slider located directly under the stage.

(3) If you want to use a color lter, swivel the lter holder out, towards

you. You can now place the desired color lter into the circular opening.

Slide the lter holder back to original position before observation.



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2-5 Using the trinocular port The AmScope T490 is uniquely designed so that you can focus the

image through the eyepieces and the trinocular port simultaneously.

This “parfocal” feature allows the images through the microscope’s

eyepieces, and those displayed on your computer screen or TV to be

viewed at the same time! You do not need an adapter to attach your

AmScope camera to the trinocular port. However, you may need an

adapter if you have a non-AmScope camera.

Attaching a camera:

(1) If your camera has a C-mount, simply screw the camera onto the

trinocular port.

(2) If your camera has a 23mm mounting size, remove the C-mount from

the top of the trinocular port by loosening the screw. Then, drop the

camera into the trinocular tube.

(3) To focus through the trinocular port, simply turn the middle portion

of the tube.

2-6 Setting the stage’s stop-limit(1) Set the stage to the maximum height desired.

(2) Pull the stop-limit knob towards the stage. The stage’s stop-limit is

now set, allowing you to move the stage down, but not up past this

point.

(3) To reset the stage’s stop-limit, simply push the stop-limit knob back

towards the back panel of the microscope. The stage’s full range of

motion is now restored.



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2-7 Adjusting focusing tension(1) To adjust the tension of the focusing knobs, rst locate the black

ridged tension-knob on the inside of the coarse focusing knob.

(2) To decrease tension, rotate the tension adjustment forward, towards

the stage. To increase tension, rotate the tension adjustment backwards,

towards the back panel of the microscope.

2-8 Changing the bulb(1) Before changing the light bulb, rst pull the plug out of the electrical

socket and wait for the lamp to cool down in order to avoid being

burned. Also, make sure to remove the eyepieces or secure them to the

microscope before turning the microscope over!

(2) Loosen the door-screw on the bottom of the microscope and pullopen the small door. Pull the old bulb out and replace it with a new one.

Close the door and tighten the screw.

2-9 Changing the fuse(1) First, remove the eyepieces or secure them to the microscope before

turning the microscope over.

(2) Loosen the door-screw on the bottom of the microscope and pull

opUsing a at-head screwdriver, unscrew the fuse located on the

bottom of the microscope.

(3)Remove the old fuse and replace with a new one. Screw the fuse back

in place.



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2-10 Maintenance

(+) All glass surfaces must always be kept clean. Fine dust on the

optical surface should be blown o using a hand blower or gently

wiped o with a soft lens tissue. Carefully wipe o oil or ngerprints on

lens surfaces using tissue moistened with a small amount of 3:7 alcohol

to ether mixture

(+) Do not use this solution to wipe the surface of other components.

These parts, especially those made of plastic, should be cleaned with a

neutral detergent.

(+) Do not assemble or disassemble the microscope’s electrical compo-

nents yourself.

(+) After use, cover the microscope with the provided dustcover. Make

sure to keep it in a dry and clean place in order to prevent rust.



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3. Specications 490 SERIES

Specications for the 490 Series Biological Microscope

PARTS SPECIFICATIONS WF10X/18mm

WF10X/18mm with pointerWF10X/18mm with reticle

WF16X/13mm

WFEyepiece

WF20X/10mm

X5P

X01P

PlanEyepiece

X61P

4X/0.10

10X/0.25

40X(spring)/0.65

60X(spring)/0.85

DINAchromaticObjectives

100X(spring, oil)/1.25

X4

X0140X(spring)

PlanObjectives

100X(spring, oil)

Binocular sliding head, 360° swiveling45° ViewingHead Trinocular sliding head, 360° swiveling

Binocular Gemel head, 360° swiveling30° ViewingHead Trinocular Gemel head, 360° swiveling

TrinocularPort

C-mount adjustable photo port.

Simultaneous Viewing

Focusing Coaxial focusing system

Nosepiece Quadruple nosepiece

Stage130mm x 140mm mechanical stage

Moving range: 50mm x 76mm

Condenser Abbe, NA=1.25Diaphragm Iris diaphragm

Halogen light with dimmerIllumination

ф50mm mirror

Lamp 6V/20W

Filter Blue/yellow/green

B490 T490

23mm adjustable photo port.



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4. Conguration drawing 490 SERIES

C C D

V I D E O

C A M E R A

4 0 0 P H

4 9 0 T

H 3 0 G

4 0 0 T H 4 5

4 9 0 B H 3 0 G

4 0 0 B H 4 5

4 0 0 M H 4 5

4 0 0 M S

4 0 0 B L

4 0 0 M R

4 0 0 N P 4

4 0 0 F B

A

1 0 0 X

A 4 0 X

A 1 0 X

A 4 X

P A 1 0 0 X

P A 6 0 X

P A 4 0 X

P A 1 0 X

P A 4 X

W F 1 0 X 1 8

W F 1 0 X 1 8 P

W F 1 5 X 1 6

W F 2 0 X 1 0

E P 5

E P 1 0

E P 1 6

4 9 0

S e

r i e s

B i o l o g i c a l M i c r o s c o p e

C o n g u r a t i o n d r a w i n g

4 0 0 C D 1 . 2

5

D R Y 1 0 0

O I L 1 0 0



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5. Optional Accessories 490 SERIES

NAME DESCRIPTION MODEL # PURPOSES5X EP5X23 For obtaining 20X, 50X, 200X

and 500X magnicationpowers

20X EP20X23 For obtaining 80X, 200X, 800Xand 2000X magnicationpowers

25X EP25X23 For obtaining 250X and 2500Xmagnication powers

10X with pointer EP10X23P For easier identifying of objects

Eyepiece

10X with reticle EP10X23R For measuring objects

2X A2X For obtaining 20X and 32Xmagnication powers




Plan 4X PA4X

Plan 10X PA10X

Plan 40X PA40X

Objective

Plan 100X PA100X

For obtaining high quality highresolution images

Dry DarkeldCondenser

DK-DRY100 For obtaining low powerdarkeld imagesDarkeld

Condenser Oil DarkeldCondenser

DK-OIL100 For obtaining high powerdarkeld images

CMOS Digital

MD400 (350K)MD600 (1.3MP)MD700 (2.0MP)MD800 (3.0MP)MD900 (5.0MP)

For obtaining still or live digitalimages

CCD Digital MD1000 For obtaining high quality stillor live digital images

Camera

CCD TV Video CCD-NP

Optional Accessories There is a variety of accessories available that can enhance your

microscope’s capabilities and give it additional features. Some of them

are listed in the table below:

For live images on TV



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6. Parameters/Technical terms 490 SERIES

Aluminum case AC-400 For carrying microscope aroundCase

Hard wood case WC-400 For storage

Simple PCS Phase contrast imagesPhaseContrast Kit Turret PCT High quality phase contrast

images

Stage Warmer TCS-100 For controlling stagetemperature

FluorescentKit

FK-YX Fluorescent images

Please nd more accessories on our website at: www.AmScope.com

6-1 Objectives

6-2 Eyepieces

Type MagnicationNumericalAperture

(N.A.) Medium

Par-focalDistance

(mm)

MagnicationMarks

(color ring)

4X 0.10 Air 45 Red10X 0.25 Air 45 Yellow

40X 0.65 Air 45 Light blue

60X 0.85 Air 45 Deep blue

DINAchromaticObjective(195mm)

100X 1.25 Cedaroil 45 White

4X 0.10 Air 45 Red

10X 0.25 Air 45 Yellow

40X 0.65 Air 45 Light blue

PlanObjective(195mm)

100X 1.25Cedar

oil45 White

Type Wide eld eyepiece Plan eyepiece

Magnication 10X 15X 20X 5X 10X 16X

Field of view(mm)

ф18 ф13 ф11 ф18 ф18 ф15

Optional Accessories cont’d...



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6. Parameter/technical terms 490 SERIES

6-3 Electrical system There are two options for electrical systems. Both are equipped with

6V/20W halogen lamps.

(1) 220V∼240V power supply: 220V∼240V ±10%, 50Hz

This electrical system is CE and GS certied

(2) 100V∼120V power supply: 100V∼120V ±10%, 60Hz

This electrical system is UL certied

6-4 Parameters(1) Total magnication: 20X∼1600X

(2) Field of view: ф0.8mm∼ф4.5mm

(2) Mechanical tube length: 160mm

(4) Object to primary image distance: 195mm

(5) Fine focusing sensitivity: 0.002mm

6-5 Technical terms(1) Total magnication= magnication of objective multiplied by magni-

cation of eyepiece

(2) Field of view= line field of view of the eyepiece divided by themagnification of the objective

(3) Object to primary image distance= n·sinα (max), N.A. is an important

parameter that marks the features of the objective and condenser. The “n”

is the refractive index of the medium (air or immersion oil) between the

·



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6. Parameter/technical terms 490 SERIES

objective lens and the specimen. The “α ” is 1/2 the aperture angle.

The larger the N.A., the high the resolution of the objective

(4) Object to primary image distance = the distance between the

object plane to the primary image plane. The conjugate distance isxed

(5) Mechanical tube length = the distance between the objective

shoulder and the ocular shoulder



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7. Troubleshooting 490 SERIES

Troubleshooting for the 490 Series Biological Microscope

O P T I C A L I S S U E S

The nosepiece is misaligned

There is no cover glass on the slide

The cover glass is not standard

The specimen is upside down

SYMPTOM CAUSE REMEDY

One side of theeld of view is

darker

Turn the nosepiece until it

clicks into place

Stain or dust has accumulated on the

condenser, object, eyepieces, or base

lens

Obstructions

are observed in

the eld of

view

Clean the lens

Stains has accumulated on the

specimen

Stains have accumulated on the lens

Clean the specimen

Clean the lens

Unclear

Image

The immersion oil has accumulated on

the dry objective

No immersion oil used with the 100Xobjective

Air bubble in the immersion oil

Used wrong oil

The aperature is not open to an

appropriate diameter


lens in the inlet of the head

The condenser is not in the r ight position

Add the cover glass

Use glass w/0.17mm thickness

Flip it over

Clean lens thoroughly

Use immersion oil

Pop the air bubbles

Adjust the iris diaphragm

Use standard cedarwood oil

Clean the lens

Adjust the condenser

One side of the

eld of view is

dark or the

image moves

while focusing

The eld of

view is not

bright enough

The specimen slide is not xed

The nosepiece is not in the right position

The iris diaphragm is not big enough

The condenser is not in the right position


condenser, objective, eyepieces, or base lens

Secure slide to the stage with

clips

Turn the nosepiece until it

clicks into place

Adjust the diaphragm

Adjust the condenser

Clean the lens



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7. Troubleshooting 490 SERIES

Troubleshooting for the 490 Series Biological Microscope cont’d...

O P T I C A L I S S U E S

The mechanical stage is not properly secured

Tension knob is too tight

SYMPTOM CAUSE REMEDY

The color of the

image is notaccurate

Adjust the brightness-

adjustment knob

No lter is used

The objective

touches the

cover glass

Use desired lter

The slide is not secured correctly Adjust the slide

The coarse-focusing knob

won’t raise the

stage

The tension knob is too loose

Limit-stop knob locked

Limit-stop knob locked

Tighten the mechanical stage

Loosen it a little

Tighten it a little

Loosen the limit-stop knob

and lock it after refocusing

The

microscope

does not light

up

The bulb burns

out frequently

The bulb is not inserted correctly

The bulb burned out

The fuse burned out

The voltage is too high

Used wrong bulb

Insert it correctly

Replace the bulb

Replace the fuse

Use correct power supply

Replace w/correct bulb

The brightness-adjustment knob is not

in the right position

M E C H A N I C A L I S S U E S

The cover glass is upside down

The cover glass is not standard Use glass w/0.17mm thickness

Flip the cover glass over

Unable to

move the slide

smoothly

The microscope is unplugged

Check the power cable ‘s plug

Focus-knob

does not turn

Stage declines

by itself

The ne-

focusing knob

won’t raise the

stage

Loosen the limit-stop knob

and lock it after refocusing

E L E C T R I C A L I S S U E S

The bulb

ickers The bulb is close to burning out Replace the bulb

The fuse burns

out frequently The voltage is too high

Use the correct power supply

or get a voltage adapter

490series_completemanual

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