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ng a step fnnovation topective relatioainable Agro-

different linbiodiversity, forestry co-paterials, agri-e projects cu

embrane ttharanthus

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ar antagonismed by gel-shisation of theassays in to

y RT-PCR analysis. s over DIV iss interact insomain, wherenuclear localil identity. Thn such a vaimplicated in

AB

ms using thea multidiscip

and chemistr

forward in to the convenons to enviro-food Chainses of reseasustainable

products. Op-food and fourrently unde

transport os roseus (

                     kshop Anual Bi18 / 19 Julh

m that RAD hift assays ese three probacco epid

nd the evolut

s mediated bside the nuclee RAD is preisation, prevhese results ariety of landn a broader

e production-plinary teamry to agrono

the researchntional prodonment. s and Biosyrch and the ecosystem

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of the higL) G. Don

                     

8 

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exerts in the

roteins dermal ionary

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sured by PAMators of stressevere drougnology. DataRNASeq anaify over 300 ght toleranceect funding by

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rsity, Functiono, Campus de fruticultura Suigaciones Cienlaga, Campus

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p.edu.pt/bio

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al species fros. Mainly duepecies and rature, high

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proteins thang a fundame3/2009 and P

velopment

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antly changinized a myria

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ng environmead of strategciated to env

st and reversquitin and u

ay exert diffeor even creattachment oULP/SENP faE2 conjugasely be carriednuclear-relateent in plants lethality or

ns, tissues angation and d

components y large geneng different verse geneten characterd the respons012 and FCOMP

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sponse to

Plant Function

uguese Medeconomic vaspecies of

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stress. Photent were useroots in respequencing ushed Q. subxpression. Tto play a keyor new functiGPL/118505/

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Plant FunctionC., D.C., S.F.,Universidad

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ents that comies to adaptvironmental rsible regulatobiquitin-like rent effects

eating or bloor sumoylatioamily), and te, aided by Sd out by the ed functionssince loss-ofpleiotropic

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drought

nal Biology C

diterranean falue, cork oa

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mpromise sut, avoid or toresponses isor of key promodifiers, ndepending oocking interon requires Sthen conjugaSUMO E3 ligSUMO prote

s, and are pof-function m

defects. Smental stageson machineryundant in thed are the poactivity, subcy, several sets place UL

nmental stresDER-028459).

                     

9 

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Center,

forest, ak has limate on are resent plants

activity ogical derate

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Anti-bacteusing With

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e present sania somnifeation, size aniques such oscopy (SEMersive specthesised AgNe. AgNPs exas plant pafaciens) comaction study eria. The coolics and wh

Antioxida

edo C, Dias M

rtment of Biolpus Universitáespondig auth

work aims tohotosynthetizedarach wed, plant pegrowth, chl

ties of antioxperoxidation

CO2 assimilaophyll cells (th (dry weignduced an uuction of antased antioxid

used in re/aesting to anasitive strategy

 

                      

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Selvakesavan

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study, we reera aqueousnd shape ofas UV-Visib

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ompounds rhitanolids pre

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logy and CESrio de Santiag

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fluorescencemes and antysis demons) and decreaer, WS did ntion and pla

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ty, plant growprograms f

tress-inducede antioxidant

p.edu.pt/bio

ver nanopqueous lea

lin G1, Bruno S

of Agro-EnvirUniversity of MUniversity of Pces, Instituto S

een synthests as reducithesized AgNcopy, laser copy (AFM),

SEM and A0-110 nm, mher antibacte

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alysis revealefor the na

extract, as re

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for EnvironmeAveiro, Portug

water stresscinal plant Mnts at 20% ofated through, gas exchatioxidant metstrated that Wased the COot affect the

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particles gaf extract

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ronment and BMinho, PortugaPorto, PortugalSuperior de C

is of silver ing agent anNPs were coDoppler aneX-ray diffrac

FM images mainly spherrial activity (

domonas aeW. somnifered the effec

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ange, pigmetabolites, ceWS induced

O2 availabilitye photosynthWS increaseymes, CAT, A

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of the antioxidof this specie

          4ª Work

green synt

Alberto C.P. D

Biological Scial l

Ciências da Sa

nanoparticlend their chaonfirmed by emometry, Sction (XRD)

confirmed rical and soup to 200x) ruginosa anra leaf extr

ctive uptake formation wPLC analysis

water defi

rine Studies, U

he antioxidanach L. Two mity) during 2rement of thnt content, Hll membranestomatal clo

y in the interetic efficiencd cell membAPX and Gr photosynthepporting thatitats. Moreodant capacitys.

                     kshop Anual Bi18 / 19 Julh

thesised

Dias 1

iences (CITAB

aúde-Norte, G

es (AgNPs) aracterization

physical-cheScanning Eleand X-ray ethat the si

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were identifies.

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University of A

nt capacity amonth old pla20 days. Aftehe water potH2O2, proline permeabilitosure, reducercellular spaccy of PSII orbrane permer and also anetic apparatut this species

over, it wouty may be us

                     

10 

ioplant o 2013 

B-UM),

Gandra,

using n. The emical ectron energy ize of agonal

man as terium ellular by the ed as

tions

Aveiro,

and on ants of er this ential, e, the ty and ed the ces of r plant eability n over s and s may

uld be sed as

                       

 

          

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artamento de Bstério da Educ

a azedarach,ercial, e apree. Esta espéudo, Timor-L

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ação de zoento e fot

Glória Pinto1,

Biologia, Univcação de Timo

, ou amargoesenta proprécie existe Leste apresa, agriculturaos florestais,ção da amarida permitiriaégico para o égia é neceso, usaram-ssementes e

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onas degrtossíntese

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versidade de Aor-Leste, Dili, T

seira, é umariedades medem quase t

senta problea itinerante, , proteger prgoseira na aa melhorar aDesenvolvim

ssário avaliase sementese de plantas sementes e

rminação daseso das raízfotossintética

u também o de luz pelosentaram umamuito sensívpreliminares snão seja sufi

p.edu.pt/bio

radadas pe

antos1

Aveiro, 3810-1Timor-Leste

a espécie lendicinais utiliztodas as remas crescedesmatamenpráticas etnoarborização a qualidade

mento (PED)r o grau de

s e plantas s foram expe plantas fos sementes

zes). A sobrea, e reduziu

conteúdo ds PSI/PSII. Ha tendência vel a stressugerem queiciente para

plant 

pela seca c

193, Aveiro Po

nhosa que pzadas na tragiões do pa

entes de degnto descontrobotânicas de solos degde vida e a do Governosuscetibilidacom 2-3 mepostos a coram expostoe afetou de evivência dao índice de a

de pigmentoHouve uma te

de decréscise, analisoue a fotossíntcompromete

          4ª Work

com amar

ortugal

produz madeadição etnobaís –litorais gradação derolado, etc. Ae combater

gradados pelauxiliaria a imo Leste-Timoade da amareses de idadndições normos a stresse

forma difereas plantas nabertura estos, o que poendência pamo com o su-se tambémese na amar

er a viabilidad

                     kshop Anual Bi18 / 19 Julh

rgoseira:

eira com intebotânica de T

e montanhoe solos devA necessidar a desertifila seca em Tmplementaç

orense. rgoseira a stde, a crescemais de irrig hídrico (20ente as raízenão foi afetaomática, limode justificarara aumentarstresse. Senm a degradrgoseira é afde das plant

                     

11 

ioplant o 2013 

eresse Timor-osas-. vido a de de cação Timor-ão do

tresse er em gação %). O es e a da. O itando r uma r o F0

ndo os dação fetada as.

                       

 

          

O6 g

Albuq 1- CIBCamp2- CICPorto,3- ISE4- CMPorto,5- FCPanor RalstseverIn ordneed high methconceIn thidifferidentinuclemarktargeal. [2throusimulsuch infrasepidedisse Refer1- Viappro44: 52- AlEvolueuves

                     

Dot-blot vgenotypin

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BIO- Centro pus Agrário deCGE-Centro d, Porto, PortugEC-Instituto SuMUP-Centro d, Porto, Portug

CUP- Faculdadrâmica nº 36,

tonia solanacral crops, nader to enforcto develop sgenotypic dods presenerning their ss work, noveent races aified using t

eotide sequeers were ass

et species us]. The data ghout the dltaneous useas inverted

subspecific demiologic paemination mo

rences: ieira J, Menoach for the 06-512. lbuquerque Putionary and sicatoria in p

 

                      

validation ng of Rals

aridade CMR2

de Investigaçe Vairão, Vairãde Investigaçãgal uperior de Engde Matemáticgal de de CiênciaPorto, Portuga

cearum (Rs)mely potato,

ce large-scalspecific deteiversity obse

nt several aspecificity, deel Rs specificnd biovars, the Pfam daences, as prsessed for thsing PCR anobtained shoifferent testee of broad sdot blots, pr

discriminationatterns whichodels.

ndes MV, Aidentificatio

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plant samples

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of novel Dtonia sola

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ção em Biodião, Portugal ão em Ciência

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as, Departameal

) is a Gram- tomato, bane monitoring

ection and tyerved within advantages etection resoc markers, awere select

atabase andreviously deheir specificitnd hybridizatowed that wed Rs strainsspectrum anrovides discrn of Rs isolath are importa

lbuquerque n of bacteria

CMR, Rodrigal assessmens. PLoS ONE

p.edu.pt/bio

DNA markanacearum

S2,4, Tavares

iversidade e

as Geo-Espac

Coimbra, Coimsidade do Po

ento de Biolo

-negative rodnana and tobg and quaranping methodthe Rs speover cultur

olution and aable to detected and valid a follow-uescribed [1]. ty and consition-based a

while some ms, the major

nd strain-speiminatory hytes. Ultimateant to imple

P, Moradasal taxa-spec

gues AS, Mant of novel mE 7(5): e378

plant 

kers for dem species

F1,5

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ciais, Faculda

mbra, Portugalrto, Faculdad

ogia, Universid

d that causebacco, causinntine phytosads for this paecies complere-based mssay time.

ct and discrimidated. Rs-s

up BLAST aThe 17 no

stency acrospproaches a

markers wererity displayedecific markerybridization pely, this workment suitabl

s-Ferreira P,ific molecula

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36. doi:10.13

          4ª Work

etection ancomplex

enéticos, Univ

de de Ciência

de de Ciência

dade do Porto

es systemic ng importantanitary procethogen, part

ex. In this reethods of

minate Rs stpecific prote

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ss diverse Rsas detailed be broad specd strain-specrs in high-th

patterns usefk might contrle control me

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ruz J, et al. (etection of X371/journal.p

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nd

versidade do

as, Universida

as, Universida

o, Edifício FC

wilting diseat economic loedures there ticularly due egard, DNA-detection, m

trains belongein domainsthe correspo

Rs-specifics strains and

by Albuquerqctrum, i.e. prcific patternshroughput asful for presumribute to deteeasures and

F. (2007) A Lett. App. M

(2012) Xanthomonaspone.003783

                     

12 

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Porto,

ade do

ade do

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ase in osses. is the to the based mainly

ging to were

onding c DNA d non-que et resent s. The ssays, mptive ermine d infer

novel Microb.

s 36

                       

 

          

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o MartaMarisa 1 CIMPorto,2 CIMRua d Someestuaaim oaustrFor thwere light irrigatoptimsalinesoluti(prepmontaboveseparboth belowseempotenenhabe a saltm Ackn [1] Alplantsand S

                     

Contributof cadmiu

a Nunes da Sa R. Almeida2

MAR/CIIMAR, , Portugal.

MAR/CIIMAR –dos Bragas, 28

e salt marsharine areas [of this work ralis plants inhis study, plaplaced in veconditions. tion system

mum nutritione Cd solutionion containin

pared in the hs in the aeground tissrated from tplants wer

wground tissumed to promntial of this nced. Therevaluable str

marshes, bein

owledgment

lmeida C. M.s on metal rShelf Science

 

                      

tion of theum in estu

Silva1, Ana P. 2

Faculdade de

– Centro Inter89, 4050-123

h plants hav1], being impwas to eva

nfluenced Cdants of both essels and mA nutritive s(2 daily cyc

nal conditionn, which wasng an autochlaboratory) w

abovementiosues were sethe sedimenre able to ues, without

mote Cd tranplant. In ad

efore, the addrategy to potng useful for

ts: To FCT, P

. R., Mucha Aretention in ae, 91, 243-24

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e plant rhizarine area

Mucha2, A.

e Ciências, Un

rdisciplinar dePorto, Portuga

e already shportant to st

aluate how td removal frospecies, col

maintained insaline solutiocles of flood ns. After 2 ws in contact whthonous enwas added tned conditio

eparated fromt. Cadmium accumulate showing sig

nslocation toddition, the dition of autotentiate the athe recovery

Portugal, for

A. P., Vascoan urban est49.

p.edu.pt/bio

zosphere as 

Cristina Roch

niversidade do

e Investigaçãoal.

hown to havudy and testhe rhizosph

om sedimentslected togethn greenhouson was add/ draught) t

weeks of acwith the plantnriched consto half of theons, being am belowgrou

was determconsiderab

gns of toxicityo P. australphytostabilizochthonous ability of they of moderate

PTDC/MAR/

oncelos M. Ttuary (Lima

plant 

system to

ha1, Catarina

o Porto, Rua

o Marinha e A

ve potential t strategies tere of Juncus favoring enher with the

ses, exposedded to all veto mimic theclimation, alt rhizospheresortium of me vessels. Vafterwards d

und structuremined as befble amountsy. The additiolis stems, inzation potentmicroorgani

ese plants toely impacted

/099140/200

T. S. D. (2011estuary, NW

          4ª Work

o the phyto

Teixeira2, Ca

do Campo Al

Ambiental, Uni

for metal phto enhance tus maritimusnvironmentalsediment inv

d to natural eessels throue tides and ml vessels we

e for about 6hicroorganism

Vessels weredisassemble.s, which in tfore [1]. Ress of Cd, eson of the micncreasing thtial of J. masms resistan

o phytoremedd estuaries.

8.

1). Role of dW Portugal).

                     kshop Anual Bi18 / 19 Julh

oremediat

arlos R. Gome

legre, s/n, 416

iversidade do

hytoremediatthat potentias and Phragl decontaminvolving their environmentagh an autommaintain plaere spiked wh. Afterwards

ms resistant e maintained. For that, turn were casults suggesspecially incrobial conso

he phytoextraritimus wasnt to Cd seediate this me

ifferent salt mEstuarine C

                     

13 

ioplant o 2013 

tion

es1, C.

69-007

Porto,

tion in al. The gmites nation. roots,

al and mated

ants at with a s a to Cd

d for 2 plants refully st that

their ortium raction s also ems to etal in

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to B.1,2, Pereir

IG – Centre dade de Ciênc

ecular Biotech-517 Coimbra,

rticular charao-acids long

se physiologain is characices linked t

cking has alroplasts expreotein sortinghave been

ctional molecently, a noverevealed a see PSI domain

reported forising model

ein sorting. Rains, from a ional diversiul from a biot

A highly eanticance

cia Duarte1*, D

MC – Instituto -180 Porto, Popartamento departamento de-007 Porto, Po

aranthus ross) vinblastineynthetic stepwledge on theent, several s, urging tacterization. ancer TIAs iional analysitransformatiowhere the

iple of the Prx1, a classession of diffeminal peptiformation prplant-transfe

acterization o

 

                      

t Specific

ra C.1, Veríssi

for Biodiverscias da Univernology Labor Portugal.

acteristic of p insertion, hical function

cterized by ato each otheready been

essing a PSI- to the plandemonstrat

cules, actingel AP has beeries of intrign, as well asr typical aspa

for studyinRecent work

bioinformatiity associatetechnologica

efficient leer alkaloid

Diana Ribeiro1,

de Biologia Mortugal e Biologia, Unie Biologia, Facortugal

seus leaves e and vincrps and regue TIA biosyntC. roseus tthe developLeaves are

is completedis. Here, a hon is reportedbiosynthesisapplication s III peroxiderent CroPrxide signal. ocedure seeerable tool of the wealth

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plant asparticighly homolois currently

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accumulate ristine, and ulatory genethetic pathwaranscriptomipment of ee the singled, and therefhighly efficiend, enabling t

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nal and Integrorto, Rua do Cr for Neuroscie

c proteinaseogous to bot

y unknown –cked globuladisulfide bridged in transietepsin. Howehis domain’st that suggembrane-destred in Chlams, such as astidial subce

ses, turning tcular mechaup, with the

mical and cel-mediated pw.

plast expresm in Cath

eijeiro1,3 and M

Celular, Univer

Minho, Campiências da Uni

in low levelsintense res

es. Howeveray, its regulaic projects aeasy and ee C. roseusfore mesophnt method foto reproducibmulation of tecular tool, ed in TIA bons revealed tomized op

asily adaptabbe applied te genes eme

plant 

cular Role

rative GenomCampo Alegre,ence and Cel

s is the presth saposins

– the Plant Sar structure cges.This dom

ent expressioever, additio

s properties ests plant atabilizing age

mydomonas rn 80 amino-a

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anisms assoe objective olular perspec

protein sortin

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rsidade do Po

pus de Gualtariversidade do

s the anticanearch has r, there areation and TIAare unveilingefficient mos organ whhyll cells areor C. roseusbly test TIA cthe anticance

the subcellbiosynthesis,

that CroPrxptimization ole to differento non-modeerging from n

          4ª Work

e in Protein

mics, Departam s/nº, 4169-00ll Biology, Un

sence of an aand saposin

Specific Insecomprised bymains’ imporon experimennally to the in inducing vspartic proteents and protreinhardtii, itacid long alaation, both ooteinase, chlociated with of further chactives have

ng routes, w

stem for ths roseus

mayor1,3,*

orto, Rua do C

r, 4710-057 BrPorto, Rua do

ncer terpenouncovered a

still enormA transmemb a high num

olecular toohere the bio

the ideal tamesophyll p

candidate geer TIAs occular sorting

was invest1 is sorted toof protoplas

nt species, suel species fnext generati

                     kshop Anual Bi18 / 19 Julh

n Sorting

mento de Bi07, Porto, Porniversity of Co

approximaten-like proteinert (PSI). Thiy five amphirtance in vacnts using toPSI’s involvevesicle leakaeinases migteinases. ts characterianine-rich insof which had lapsin, into athe PSI’s r

aracterizing recently ide

which could

he study o

Campo Alegre

raga, Portugao Campo Aleg

id indole alka number o

mous gaps ibrane transpmber of candols for funcosynthesis oarget for TIAprotoplast isoenes in the pcur. As a pr

determinatitigated. Trao the vacuolest isolationuggesting thfor the funcon sequenci

                     

14 

ioplant o 2013 

iologia, rtugal. oimbra,

ly 100 ns and is PSI pathic cuolar bacco ement age in ght be

zation sertion never

a most role in these ntified prove

of

e, 823,

l gre s/n,

kaloids of TIA in the ort. At didate ctional of the

A gene olation recise oof of ion of nsient e by a , the at this ctional ng.

                       

 

          

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Richa 1 Cent2 GruDepar3 Dep(BioFI4 Cent Photochlorocharachloropulsespecifluorequantcapacfluorepresephotointensundeindicaexposinvolvaccum 1 - BImagPhoto AcknOperfor SEuropwas sproje

                     

0 Photosyimaging

ard Breia1,2, Só

tro de Investigupo de Invesrtamento de Bpartamento deIG), Faculdadtro de Oceano

osynthesis isophyllous duacterize the ophyll fluore

e (SP) intenific distributescence (Fmtum yield (Φcity and theescence signented a highoinhibition. Gsities. Abover light-adaptating a photsure to saturvement of tismulation of o

Breia et al. ing: The Effobiology, 89:

owledgmentrational ComScience andpean COST supported thct PTDC/AG

 

                      

ynthesis inPAM fluo

ónia Vieira3,4,

gação e de Testigação em Biologia, Escole Biologia Ve

de de Ciênciasografia, Faculd

s not exclusivuring early

photosynthescence imansity on phoion pattern

m) and in raΦII). After R lowest phonals and ph

h photochemGlobally, all tie 5000 μmol ted conditiontoinhibitory inrating pulsesssue-specificorganic comp

2013. Mapffect of Satu: 579–585.

ts: This workpetitiveness d Technologaction FA 1

hrough a reseGR-ALI/10063

    www.map

n grape berometry

Jorge Marque

ecnologias AgrBiologia Veg

la de Ciênciasegetal e Cens da Universidadade de Ciênc

ve of green ldevelopmenetic propertiging (imagin

otochemical of photosy

apid light cuRLC modelinotoinhibition shotochemicaical capacityssues saturam-2 s-1, Fm, F

ns were susnteraction bes (1). These c photosynthpounds durin

pping Grape urating Pulse

k was suppoProgramme

gy) under 1106 and thearch grant 36/2008.

p.edu.pt/bio

erry tissue

es da Silva3, H

ro-ambientais getal Aplicadas Universidadentro de Biodivade de Lisboacias da Univer

leaves. In fantal phases, ies of the gng-PAM) waparameters

ynthesis andurves (RLC)ng, the exocsusceptibilityl competency, similar to ated the PSIF’m and ΦII wsceptible at letween SP results open

hesis in the ng grape berr

Berry Phote Intensity i

orted by Eure) and by nathe project

he research fellowship (U

plant 

es: an in v

Hernâni Gerós

e Biológicas Ca e Inovaçãoe do Minho, Bversidade, Gea, Lisboa, Portrsidade de Lis

ct, some sincould exhib

green grapeas used. Als

was testedd a strong

parameterscarp showey and the mces. Notablythe exocarpI reaction ce

were significalower saturaand actinic

n the way to highly compry developm

tosynthesis n Different

ropean Unioational funds s FCOMP-0project PTD

UMINHO/BI/

          4ª Work

vivo appro

1,2 and Ana Cu

CITAB-UM, Poo Agroalimenraga, Portugaenómica Integtugal

sboa, Lisboa, P

k tissues, likbit photosyne berry, theo the effect

d. Results reeffect of S

s, such as d the highe

mesocarp, revy, the seed , although it

enters at relaantly affectedating light (32light intensitfurther studi

partmentalizeent.

by ChlorophTissues. Ph

n Funds (FE(FCT-Portug

01-0124-FEDDC/AGR-ALI/177/2011) as

                     kshop Anual Bi18 / 19 Julh

oach by

unha1,2

Portugal ntar (AgroBioal grativa e Fun

Portugal

ke fruits, whicnthetic activite pulse ampt of the satuevealed a tSP on maxF’m and eff

est photosynvealed the louter integu

t is more proatively low SPd, but inner ti200 μmol mties and repies concernined productio

hyll Fluoreshotochemistry

EDER/COMPguese FoundDER-022692/100636/200ssociated wi

                     

15 

ioplant o 2013 

oPlant),

ncional

ch are ty. To plitude uration issue-ximum fective nthetic owest ument one to P light ssues

m-2 s-1) petitive ng the n and

cence y and

PETE-dation

2 and 8. RB ith the

                       

 

          

O11 Vivian 1CentInvestBiolog3Depa Fungupon coppemay bmetadelivecells the savoidlevel been eight VvCTrespocells succefunctiwithin AcknOperFounthe ALI/1and tEuropfundeGrap O12

Ecoin

                     

Copper t

na Martins1,2, A

tro de Investigtigação em Bigia, Escola Cartment of Pla

icides basedthe develop

er sulfate anbe a cause ol ions have ery within thdecreases w

sequestrationd its inheren

and in intermostly charputative Viti

Tr1 was inveonse to Bordafter transie

essfully restoion as a copn the cell.

owledgmentrational Comdation for S

research 00636/2008the PhD grapean projected COST Aevine Divers

- Desenvcombat

side

 

                      

ransporte

António Teixe

gação e de Tiologia VegetaCiências, Univnt Sciences, U

d on copper spment of thend lime. Desof toxicity, anevolved to ae cell compa

with the incren of the mett toxicity. Co

rnal membraracterized inis vinifera Coestigated in deaux mixturent transformored the growpper transpo

ts: This worpetitiveness

Science and project F

), the projecant no. SFRt INNOVINE

ACTION (FAsity Exploratio

volvimentote à crise

    www.map

ers in grap

ira1,2, Elias Ba

Tecnologias Aal Aplicada e Inversidade do University of C

salts have bee ‘Bordeaux spite being and several mavoid negativartments. Pr

ease in coppetal ion in theopper transp

anes, such a yeast, humopper Transpgrapevine t

re. Furthermomation with fuwth-defect o

orter and givi

k is supportProgrammeTechnology)FCOMP-01-0ct FCT/5955/RH/BD/6458E (ref. 31177A1106 “Quaon and Mobi

o Sustentá

p.edu.pt/bio

pevine

assil3, Eduardo

Agro-Ambientanvestigação AMinho, Cam

California, One

een extensivmixture’, a an essentialechanisms sve effects atrevious studer concentrae vacuole mporters (COPas the tonop

mans and in sporters (VvCtissues throuore, the locausion protein

of yeast mutaing insights

rted by Euroe) and by po) under the 0124-FEDER/27/5/2013/S7/2009 to V75), by the lityFruit" anlization of Ad

ável e Eco

plant 

o Blumwald3,

ais e BiológicaAgroalimentar mpus de Guae Shields Ave,

vely used in vbroad spectr element fo

such as chelat cell level wies have sho

ation in a dosmay constitutPT/Ctr) operlast. These some plant s

CTrs) were idughout devealization of Vns of VvCtr1ants lacking on its contri

opean Unionortuguese naproject FCO

R-008760 S - scientific VM. This wonetworking ad FA1003 daptive Traits

oinovação

          4ª Work

Hernâni Geró

as (CITAB), P– Agrobioplanltar, Braga, 4 Davis, CA 95

viticulture sinrum fungicidr plant healtation and com

while insuringown that these-dependente an effectiate at the phigh-affinity species. In t

dentified and elopment andVvCTr1 was

with GFP aCtr transporbution for co

n Funds (FEational funds OMP-01-0124

(Refª. FCcooperation

ork is also activities wit"East-West s for Breedin

- ferrame

                     kshop Anual Bi18 / 19 Julh

ós1,2

Portugal; 2Grunt, Departame4710-057, Po5616, USA

nce the late de that consth, excess cmpartmentatg their approe viability of nt manner anive mechani

plasma memtransportersthe present the expressd ripening aassessed inand RFP. Vrters, validatopper mobili

EDER/COMP(FCT-Portug

4-FEDER-02CT PTDC/n Portugal-Tusupported bthin the euroCollaboratio

ng”).

entas de

                     

16 

ioplant o 2013 

upo de ento de ortugal;

1800s ists of

copper tion of

opriate grape

nd that sm to

mbrane s have study,

sion of and in n plant

VvCTr1 ing its zation

PETE-guese

22692, /AGR-unisia, by the opean on for

                       

 

          

O13 A.M. P 1Univ4169-2BioFBiotec The S(Solabe onThe tpublicMetasignamechTherealongThe ZZn, FfacilitcompNRAMtransCd traA larArabiidentitransdatabputatUsingmininNRAMwith oOur cdesigbut toWe ein So

                     

Identifica

Pessoa1,2, S.

versity of Porto-007 Porto, PoFIG - Center chnology Lab,

Solanaceae anum lycoperne of the motwo model pcly availablel transport a

al transductiohanisms for defore, many g with cationiZIP family (ZFe and Mn tators) transpartmentalizaMPs (naturaport and regansport abilitrge number idopsis as wified for Soporter sequebases (unknive metal trag these annong and geneMP family wother plant sconserved p

gning suitableo identify theexpect that tholanaceae pla

 

                      

ation of he

Pereira1,2 an

o, Faculty of ortugal; r for Biodiver University of

include comrsicum) and ost economicplants tomat. nd uptake oon. In additidecrease themetal transpc nutrients.

ZRT, IRT-likefrom outsid

sports a wation by puml resistance-

gulation of Fety. of heavy m

well as for olanaceae. Hences publisnown DNA cansporters. otated seque

e prediction twere identifiepecies, such

primers desige primers tham in other S

hese results wants.

    www.map

eavy meta

nd J.Pissarra1

Sciences, Bio

rsity, FunctionPorto, Portug

monly edibleEggplant (Scally importato and potat

f metals are on, plant me

e toxicity of ceporters are kn

e proteins) ple the cell in

wide range mping the me

associated me. All of thes

metal transpoother plant sHowever, thehed and alsclones, EST

ences alongtechniques,

ed and charah as Thlaspi agn tool, Primat allowed noolanaceae pwill be a step

p.edu.pt/bio

al transpor

1,2

ology Departm

nal & Integragal;

e plants sucholanum meloant and the to had their

essential foetal transpoertain metal nown to tran

lays prominento the cyto

of metal etal out of themacrophage se three fam

orters have species specere are sevso many unaTs, GSSs, g

g with the seseveral puta

acterized in and ArabidopmerIdent (htot only to con

plants as welpping stone

plant 

rters in So

ment, Edifício F

ative Genom

h as Potato (ongena). Thmost valuabgenome rec

or plant groworts systems

ions. nsport toxic c

ent roles in moplasm. The

ions and e cell or intoproteins) areilies of trans

already beecies, but onlyveral inconsiannotated Sogenome seq

equence infoative transpotomato and psis. tp://primeridnfirm the prel. in the unders

          4ª Work

olanaceae

FC4, Rua do

ics, Plant M

(Solanum tubis plant taxo

ble regardingcently seque

th, developmare a very

cations (such

metal ions upCDF familyis known

o the lumen oe known to pporters are k

en describedy a limited nstencies in

olanaceae sequences) tha

ormation fromorters from t

potato, as w

ent.up.pt), wedicted trans

standing of m

                     kshop Anual Bi18 / 19 Julh

plants

Campo Alegr

Molecular Biol

berosum), Toon is consideg vegetable enced and is

ment, nutritioimportant p

h as heavy m

ptake, transpy (cation dif

to promotof organellesparticipate inknown to po

d for Thlaspnumber has the heavy

equences in at may repr

m NCBI, andthe ZIP, CDwell as com

was then useporter seque

metal homeo

                     

17 

ioplant o 2013 

re, s/n,

logy &

omato red to crops. s now

on and part of

metals)

porting ffusion te Zn s. The nto the ossess

pi and been metal NCBI

resent

d data F and

mpared

ed for ences,

ostasis

                       

 

          

Pos___ P1 P

r Artur 1 Cent2 GruDepar3Instit4InstitPortug Polyoas osand sTOF-leavewatersorbitfull malso condiby Vvapprostressbiomaunde(1) CAcknOperfor SEuropPTDC

                     

sters ______

Polyol traresponse

Conde1,2, Ana

tro de Investigupo de Invesrtamento de Btuto de Tecnoltuto Superior gal

ols, or sugarsmoprotectansalinity, whe-MS analysises and berryr deficit in thetol oxidation

mature stage severely reitions. ThesevPLT1. The oaches provis. Additionalarker for dror water deficonde et al. (2owledgment

rational ComScience andpean COST C/AGR-AAM

 

                      

_______

nsport anto water s

a Regalado3, D

gação e de Testigação em Biologia, Escollogia Química de Agronomia

r alcohols, arnts. In Olea re mannitol

s revealed thy mesocarp e final maturto fructose vof grape be

pressed (4-fe data are we

combinatioided new insly, increased

ought-tolerancit. 2011) Plant Cts: This workpetitiveness d Technologaction FA 1/099154/200

    www.map

______

d metabostress

Diana Rodrigu

ecnologias AgrBiologia Veg

la de Ciênciase Biológica, A

a, Universidad

re widely diseuropaea, mtransport an

hat several pin significan

ration stagesvia sorbitol derries under fold) in the ell in line witn of metabosights on thed concentratint grapevine

Cell Physiol k was suppo

Programmegy) under

1106 and the08. AC was s

p.edu.pt/bio

______

olism adjus

ues2, M. Manu

ro-ambientais getal Aplicadas UniversidadeApartado 127,de Técnica de

stributed commannitol is annd intracellulapolyols are pnt concentras of berry devdehydrogenadrought. Mamature and

th our previoolomic, molee role of polyions of polyocultivars an

52(10):1766orted by Eure) and by nathe project

e research psupported by

plant 

_______

stments in

uela Chaves3,4

e Biológicas Ca e Inovaçãoe do Minho, B 2781-901 Oee Lisboa, Tap

mpounds amn important oar oxidation

present in grations. Severvelopment. Aases (VvSDHannitol dehydd full maturus observati

ecular biologyols in grapeols in grape d a bioindica

6-75 ropean Unioational funds s FCOMP-0

projects PTDy the PhD gra

          4ª Work

______

n Vitis vin

and Hernâni

CITAB-UM, Poo Agroalimenraga, Portuga

eiras, Portugalpada da Ajuda

mong plants aosmoprotectare well cooapevine (Vitiral polyols aA significant Hs) in mesocdrogenase (Ve stages unons regardin

gy and traditevine defensberries may ator of plant

n Funds (FE(FCT-Portug

01-0124-FEDDC/AGR-ALI/ant no. SFRH

                     kshop Anual Bi18 / 19 Julh

_______

nifera in

Gerós1,2

Portugal ntar (AgroBioal l a, 1349-017 L

and frequenttant under drordinated (1)is vinifera) maccumulate dinhibition (50

carp is clear VvMTD) actinder water ng polyol trantional biochese against drconstitute a

t health/adap

EDER/COMPguese FoundDER-022692/100636/200H/BD/47699/

                     

18 

ioplant o 2013 

__

oPlant),

Lisboa,

tly act rought ). GC-

mature during 0%) of in the vity is deficit nsport emical rought novel

ptation

PETE-dation

2 and 8 and /2008.

                       

 

          

P2 R

M Rita S 1 Facu2 Cen4169-3 LabUnive4 Alter Curreuses,acknoworldare csizesspatiaspecirealisHoffmproteare ohabitaof pretools potenminimoverepresubetteapproendaimpro A. LoPost “ProgGovegrantcondi

                     

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uldade de Ciêntro de Investi-007 Porto, Poboratoire de Bersité de Lausarra Wageninge

ent patterns , with its imowledged. R

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s. Even thougal accuracy ies occurrenstically captumanns. & Liected under tobliged to unats and specedictive modto identify

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of species dmpacts on Rare species

these specied by restrictedgh datasets and lack of ces to enviroure a largenk is an enhe EU Habitndertake mocies of Commdels as asseenvironment

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emic Enda

toine Guisan 3,

rsidade do Poodiversidade e

la Conservatint de Biologie,and Research

istributions abiodiversityare a growi

es are assumd geographicon rare specvalid absen

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onitoring, assmunity intereessment tooltal conditionnica micranthand specieses distributissessment oecology and

p forward inas well as i

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e Portugues80747/2011

tores de CoFundação p

ors, methodsecosystems i

p.edu.pt/bio

angered Pl

, João Honrad

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ion (LBC), D, CH-1015 Lauh Centre, NL-6

are largely dand associing concern med to undec ranges, hacies distribut

nces, the devscriptors hav

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est. Thereby, ls to effectivns within whha was estims distributionon, wherea

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plant 

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Campo AlegrGenéticos (CIB

Département dusanne, Switz6708 Wagenin

etermined byated ecosyin the conte

ergo higher rbitat specialition usually hvelopment ove been provs ecologicas, endemic cle 17 of thisnd reporting this study a

vely fulfil thehich populatimated basedn modelling.s the latter ution of the of the specof the dist

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nd Technoloy was partiale – COMPE

cia e a Tecntocols for rescapes” (PTD

          4ª Work

es Using

Lomba 2,3,4

re, s/n, 4169-BIO), Rua do

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y changes instem servicxt of biodiveisk of extinctzation and/ohave few obsf predictive mved to be pal niche. Veof the Iberia

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DC/AGR-AAM

                     kshop Anual Bi18 / 19 Julh

Predictive

-007 Porto, PoCampo Alegr

d'Evolution

therlands

n climate andces being wersity consertion. Rare sp

or small popuservations, lmodels that

articularly useronica micran PeninsulaEU Member Sation status ess the usefubligations, a

e maintainederent approaer resulted

more conservile also allowore, the proterns of entive projectio

ion (FCT) thy FEDER thy the Portughe form of pd monitorinM/104819/20

                     

19 

ioplant o 2013 

e

ortugal re, s/n,

(DEE),

d land widely rvation pecies ulation imited relate eful to rantha a and States for all

ulness nd as

d. The aches: in an

vative, wing a posed demic

ons to

hrough hrough guese project g the 008).

                       

 

          

P3 P

d Ana S 1CentRua C2FacuAlegre3Alterr4DépaLausa KnowenhaA totaPortuand m1000assemcommcommwere areaswithinand fassemthe oforestcommheterapperegulsuggeand eResuassemprovis AcknFounfundeand bin themonitAAM/

                     

Plant specdairy-farm

Sofia Vaz1, Joã

tro de InvestigCampo Alegreuldade de Ciêe s/n, 4169-00rra Wageningeartment d’Ecoanne, Switzerl

wledge of plnce efficient al of 50 stan

ugal, and clamixed standm2 squares mblages andmunity, plantmunity, asse

mapped ans and highesn each standforest type. mblages revother forestst naturalness

munity specrogeneity anared to reflearities in Pests that struecosystem pults highlight mblages, sosions of ecos

owledgemendation (FCT

ed by FEDERby the Portuge form of protoring the c/104819/200

 

                      

cies assemmlands of t

ão Honrado1,2

gação em Bioe s/n, 4169-00ências (Depart07 Porto, Portuen University aologie et d’Evland

ant assembmanagemen

nds were surassified conss with both centred in t

d species-aret assemblagemblages wend analysed st species ricd. Segregatio

Still, randovealed trends. Overall, res on plant divcies richnesnd low disturect shifts in

Pinus, and fuctural chanrocesses and

forest natu extensively system servi

nts: A. LomT) through PR through “Pguese Goveoject grant “condition of 08).

    www.map

mblages athe Northw

2 and Angela L

diversidade e7 Porto, Portutamento de Bugal and Research olution (DEE)

lages is essnt and conserveyed alongsidering theirspecies. Vathe stand ceea relationshes were assre explored wthrough po

chness. For on patterns pm patterns

s for regular esults converversity. Pinuss and asrbance level the manag

fine-scale agges within std services.

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mba is suppost Doc Gra

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p.edu.pt/bio

along a grwestern P

Lomba1,3,4

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Centre, NL-6), Université d

sential to coervation meag a gradient r dominant t

ascular plantentroid, and hips were evsessed calcuwithin 100m2

oint pattern aall forest typ

prevailed in were foundpatterns in P

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ecological ps forests worturalness in ptus globulurded within

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ally sink orgaroved that soto fruit and suspensione berries froell growth, chpper-based fases since cerns, watersponsible for pact of thestle informatiocit conditionscrease in dryced in CSB-decreased iell growth,

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ns, so fruit pome grape bd seed devn cells derivem cv. “Alvar

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sis has been exhibit high

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minho.pt)

seldom over photochemipresent stut Sauvignonrmine the efftochemical qgainst severansive applicnt environmey. Several st in many s

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ble cell mo

Portugal ntar (AgroBioal ar, Universida

rlooked. Howical activity,

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dre Armando

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                     kshop Anual Bi18 / 19 Julh

nt in plant

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human and of inadequatulation, i.e. areal-rich diein zinc deased solutiois mechanis

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ómicos superriqueza espeRA 2000, no habitats saxenquanto qu99 géneros

ara os dadoitats estudaditiva e signifiugere que e briófitas e,

sto que pod

                     

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gação em Biod4169-007 Por

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peroxidasced by ara

and Mariana

de Biologia Mortugal Biologia, Facu

ortugal

dases (Prxs)mining the apendent oxidave obtainedf Prx, and thcosidic epitopn this work, tseus to redroseus peroxits oxidation re suitable I) and compxidase-type of Prxs. Theg a higher anofluorescen

pecific AGP a punctuated

This data ass III peroxons attributed

ts: Project fuOMPETE andr the project

cenate Gran/2008 

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ses peroxabinogala

Sottomayor1,2

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uldade de Ciê

) are typicaarchitecture aation activitie

d indirect eviat Prxs are ipes implicatehe capacity oduce the caxidase 1 (Croby H2O2 andelectron do

pound II (CoII CoI and C

e Km for AGffinity of Cronce co-labeglycosidic e

d distribution strongly indidases, withd to AGPs.

nding by i) Fd by NationaFCOMP-01t from Grup

p.edu.pt/bio

xidase cataactan prote

2

elular, Univers

ências da Uni

al plant enzyand defencees leading todence that ainvolved on ted in importaof purified Aatalytic interoPrx1), the md consequenonors for thoII). SimilarlCoII, indicati

GP oxidation oPrx1 for AGelling of leaepitope and

in plasma mdicates that potential im

FEDER fundsal Funds thro-0124-FEDEpo Jerónimo

plant 

alytic inteeins

sidade do Po

versidade do

ymes that he properties o the cross-liarabinogalacthe appeara

ant aspects oGPs from thermediates omain Prx prent interaction he oxidizing y, AGPs weng that the cby CoII wa

GPs coming faf sections

an anti-Prx membrane an

AGPs are mplication fo

s through theough FCT –ER-022718 (Po Martins, a

          4ª Work

rmediates

rto, Rua do C

Porto, Rua do

have been of the plantnking of cell

ctan proteins nce /disappe

of plant devee leaves of th

of class III sent in the lewith AGPs sintermediat

ere also capcapacity to o

as lower for from the samwith a monantibody cl

nd tonoplast in vitro andr the develo

e OperationaFundação pPEst-C/SAU/and iii) the

                     kshop Anual Bi18 / 19 Julh

s CoI and

Campo Alegre

o Campo Aleg

implicated it cell wall, m wall compo

s (AGPs) areearance of seelopment andhe medicinaperoxidases

eaves of the showed that tes of CroPpable of redoxidize AGPCroPrx1 tha

me species/onoclonal anearly showeof mesophyl

d possibly inopmental an

al Competitivpara a Ciênc/LA0002/201FCT schola

                     

26 

ioplant o 2013 

CoII

e, 823,

gre s/n

n key mainly nents.

e an in everal

d plant l plant

s was plant, these

Prx1 - ducing

Ps is a an for organ. tibody

ed co-ll cells n vivo nd cell

eness cia e a 11), ii) arship

                       

 

          

P11

Ana PAlmei 1 Facu2 Cent The elemeriversproteinformPortuand eobtaininformcommanalycomm(TWINgroupprecipoccurhydrowere plannand Emanyvulnefarmscommcumu P12

A. Alm 1DepaCamp2KLÓNPortug

Pinusimpormicroconifeexogephysifor pmatucaribavisibl2,4-DinducD+BAcold growtefficieinducof eff

                     

1 Impact abryophyt

Paula Portelaida da Silva1,2

uldade de Ciêntro de Investig

objective waents (damsscapes, idenection, of flumation on bugal. Floristicecological pan the regionmation analymunities in thysed the lossmunities chaNSPAN) cops.Communipitation, annrrence were opower sche

calculated aning. BryophyEuropean biy taxa of Aerable becaus implemenmunication eulative impac

2 Effect ofPinus ell

meida1, G. Pin

artment of Biopus UniversitáN, Innovative gal

s elliottii anrtance. Ther

opropagationers. The maenous factoriology, maint

plant regenere zygotic eaea were exe after 15 d

D with 85% iction of calliAP), carbon (-15 °C), sath regulatorsent stress cction of callusfective protoc

 

                      

analysis ote commu

a1, Cristiana V

ncias da Univgação em Biod

as to condu, small hyd

ntifying simplvial bryophyryophytic coc compositioarameters ofnal communyses were pehe watershes of potentiaaracterized oncordant wties were snual averagcalculated fo

emes and theand presentytic communodiversity, w

Atlantic distruse of fragmtation.These

elements onct and conser

f different liottii x Pin

nto1*, C. Dias1,

ology,& CESArio de SantiagTechnologies

d the hybrirefore it urgen and for gein objective ors on the intenance of gration by soembryos (MZxposed to didays. The monduction of . MZE weresources (su

alicylic acid, s was 2,4-Dconditions ws in these twcols for SE.

    www.map

f riverscaunities

Vieira2, Helen

ersidade do Pdiversidade e

uct a regiondropower scle or cumulayte communommunities (n was classf sites statis

nities types erformed in Geds, establishal occurrencby 1 to 6

with many ospatialized ge temperator each comme areas of loed in a deta

nities are spawhere commibution. Esp

mentation caue results aln the assessrvation.

factors onus cariba A. Costa1, V.

M – Centre fogo, 3810-193 As from Cloning

d Pinus elles the needneral researof this studyduction of: ermplasm, momatic embrZE) and cotfferent growost efficient calli in MZEe subjected crose and mproline, H2O

D+BAP with were cold awo conifers, a

p.edu.pt/bio

pes fragm

na Hespanhol

Porto

Recursos Gen

nal assessmchemes, wiative impact nities.We use(2000-2013)

sified (TWINSstically exploand establis

GIS programhed buffers

ce of commu6 core taxof the taxaaccording tture, local munity. The

oss and poteailed map thatially restrictunities show

pecially in mused by smalowed us tsment of cu

n the calluaea Pereira2, L. M

for EnvironmeAveiro, Portugg, Biocant Pa

liottii x Pinu to develop rch (eg, phy

y was to anaa) callus tis

morphogenesryogenesis, tyledons of

wth regulatorsgrowth regu; 2,4-D andto different

maltose) andO2 and putreapproximate

and putresciand supports

plant 

mentation

l2, Bruno Mar

néticos, CIBIO

ent of impaind farms,

zones and ed a compi, from 257 SPAN, CAP)red (DCA ansh species-e (ArcMap 10of impact pe

unities.We oa and foun

a responsibto their fluvslope, altituimpacts are ntial preserv

hat allows reted in the conw high specimountain arall hydropowto analyse umulative im

us inducti

Marum2, S. Co

ntal and Maringal. E-mail: *gpark, Núcleo 4,

us caribaeaa battery o

ysiology, molyze the effesue (importasis, etc.); b) cryopreservPinus elliot

s (2,4-D, NAulators condid NAA in cot

growth regd stress factoescine. The ely 30% ratene. These

s preliminary

          4ª Work

on the con

rcos2, João H

O-UP

cts caused roads and potential zo

lation of dawatercourse) and typifiednd CCA, CAenvironment 0.1). We spaer fragmentabtained 8 tynd indicatorle for the vial Strahlerde and hilmainly due t

vation of eacgional and lntext of Portues richness eas, these

wer schemesthe impact

mpacts and

on of Pinu

orreia2, C. San

ne Studies , Upinto@ua.pt

Lote 4A, 306

have signiff in vitro cullecular bioloct of variousant in fundaembryogenic

vation studietii and Pinu

AA and TDZ)itions in indutyledons, botgulators (2,4ors, in particmost efficie

e of calli indresults suppdata toward

                     kshop Anual Bi18 / 19 Julh

nservation

Honrado1,2 &

by fragmenrailway) o

ones of ecolatabases inces in the Nod (SIMPER,

ANOCO; SPSrelations. S

atialized bryoation elemenypes of bryor pseudo-spcohesion o

r order, sulshade; areto dams and

ch communitylocal conseruguese, Iberand diversitcommunitie

s, dams andt of energyidentify are

us elliottii

ntos1

University of A

60-197 Cantan

ficant commlture protoco

ogy, etc.) of s endogenouamental studc callus (imp

es, etc). Forus elliottii x ). First calluucing callus th leading to

4-D+24-epiBrcular heat (8ent combinatduction. The port the po

ds the optimi

                     

27 

ioplant o 2013 

n of

Rubim

ntation n the ogical luding

orth of CAP)

SS) to Spatial ophytic nt and ophytic pecies of the ummer as of small y type rvation rian ty and

es are d wind y and eas of

i and

Aveiro,

nhede,

mercial ols for these

us and dies of portant r that, Pinus s was were:

o 55% r; 2,4-0 °C), tion of

more ossible zation

                       

 

          

P13

Cláud 1 DepCamp 2 CIB4485- O Vados ado Paentãodos aquímda are desentansobreindicanoroeparecpadrõdistribrecolhtrês evariamdisculocal rupes P14

Raíss 1Depa2 FHO Cr(IIIcropsvalenare bstudiemodefor 15showThis crop. all Crdosesgrowtand mthat Cstimusuppoat ver

                     

3 Relação de líquen

dia Oliveira1, J

partamento depo Alegre s/n, BIO, Centro de-661 Vairão, P

ale do Côa (anos 90 apósaleolítico Supo têm sido reafloramentosicos e biológrte rupestre.Osempenhamnto, pouco see a forma coam que Leceste, Aspicilce ser indifeões de varibuição destahidos dadosestações mem entre os 1

utida a relaçãdos fatores

stre no Vale

4 Effect of

sa CS Faria1,2,

artment of BiolO|Uniararas -

) is an esses, nuts, meance) is currenbased on theed crops for el in ecotoxic5 days and e

w a hormesispreliminary rFor that we

r doses decrs did not sigth is a resultmitosis catasCr(III) hampeulation at lowort that, contry low doses

 

                      

entre padnes domin

Joana Marques

e Biologia, Fa4169-007 Pore Investigaçã

Portugal

Vila Nova ds a descoberperior, atualmealizados divs rochosos egicos, que viOs líquenes d um papel e sabe sobremo estes atucanora pseulia hoffmaniaerente à expação de faas três espé de tempera

eteorológicas136 e os 332ão entre os p

ambientais do Côa.

f Cr III in L

Willian GD B

logy & CESAMFundação He

ential nutrienat and eggsntly considere Cr(VI) valen

Cr(VI) toxicicology. Lettuexposed to C

s effect (up toresult led us e analyzed sreased A (negnificantly afft of cell divisstrophe (micrers some ph

wer doses detrarily to Cr(Vs, despite furt

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drões ambnantes no

s1,2, Rubim Al

aculdade de rto, Portugal.o em Biodive

e Foz Côa, rta da maior mente classiversos estude superfíciessam o desedominam asimportante ne os padrõeuam sobre audistera é aana a sudesposição. Comatores ambieécies na áreatura e humids e onze sen2 metros, oriepadrões de dregistados,

Lactuca sa

Bedo1,2,C Dias

M, University ormínio Ometto

nt to humanss). Despite ted to be a tonce, while Cty is Lactuca

uce plants wCr (III) ( 20, o 20 mg/L), to raise the

some physioet photosynthfect the Ci (ion and exteronuclei) in totosynthetic

eserve furtheVI), Cr(III) hather studies

p.edu.pt/bio

bientais loVale do C

lmeida1,2

Ciências da

ersidade e Re

Nordeste dee mais impo

ificada comodos de caracs gravadas, nvolvimento

s comunidadeno processos de variaçã

a composiçãoa espécie dste e Calopm este estuentais à esea do Parqudade relativasores distribentados a nodistribuição de respetivas

ativa L. ph

1, G Pinto1, C

of Aveiro, 381o, 055 19) 354

s, being ingetheir essentioxic element

Cr(III) effects a sativa, a w

were grown h50, 150 andwith plants s hypothesis

ological paramhetic rate), a(internal CO2ension, the imthe root apex parameters

er studies. Tas potential toare ongoing

plant 

ocais e a dCôa (NE Po

Universidade

cursos Genét

e Portugal) tortante coleço património cterização da

integrando de ferramen

es que colono de deteriorão dos fatoreo destas comdominante n

placa irrubesdo pretendecala local a

ue Arqueológa de dois anbuídos pela áorte (3), sul destas espécs implicações

hysiology

Santos1

0-193 Aveiro,43-1400, Bras

ested througality to humto plants. Mremain less orldwide con

hydroponicalld 200 mg/L)showing an of Cr(III) beimeters namend led to a s2 availabilitympact of thesx is under d, and the pahe growth sto be conside.

          4ª Work

distribuiçãortugal)

do Porto, Ed

ticos, Campus

ornou-se coção de arte rumundial pela

as dinâmicasdados ambientas de gestnizam as supração dessaes ambientaimunidades. Enas superfícscens, igualme-se avaliar afetam a sugico do Valeos consecut

área de estud(6), este (1) cies e os pads para a con

Portugal il

h different fomans Cr (ind

ost studies ostudied. On

nsumed cropy on Hoagla. All indepenincrease of lng a beneficely photosynstomatal clos). Also, consse doses on iscussion. Th

athways invotimulation atered a benef

                     kshop Anual Bi18 / 19 Julh

ão de espé

difício FC4, R

s Agrário de V

onhecido em upestre ao aa UNESCO.Ds de meteorientais, geo-tão e conserperfícies gravas superfícieis à escala loEstudos antecies orientadmente abundde que form

ua abundâne do Côa. Ftivos recorredo a altitudee oeste (1). drões de vanservação d

food sourcesdependently on Cr phytotoe of the mos

p, and an excands (1/3 strndent experilengh and gcial nutrient tnthesis. Curisure. These sidering that mitotic indehese data su

olved in the gt lower doseficial nutrient

                     

28 

ioplant o 2013 

écies

Rua do

Vairão,

finais ar livre Desde zação físico-

rvação vadas es. No ocal e

eriores das a dante, ma os ncia e Foram endo a es que

Será riação a arte

s (eg., of its

oxicity st well cellent rengh) ments rowth. to this ously, Cr(III)

t plant ex (MI) upport growth s also when

                       

 

          

P15

Maria REQUUnive Presewide numehave FrombiologMacr(Chloof breconospiralwest Elevecompstimuextra51.5 the dIn coshowof α-l Tabl

a ValuRIcalc: Ackn(FCT(SFR Refer138:1Algae1-91.efficienight(http:comp58:35

                     

5 ChemicaLinnaeus

na Barbosa, J

UIMTE/Laboraersidade do Po

ently, the resvariety of

erous interesalready bee

m a human hegical activitieoalgae can

orophyta), brorown algae omic importalis Linnaeuscoast of Po

en compoundpounds. Theulated RBL-2cts. Prelimin± 8.56 % (megranulation

onclusion, in wed better an

inoleic acid (

le 1 - Metabo

ues are expr: Retention in

owledgemenT): (PEst-C/RH/BD/63852

rences: [1] A1819-1828; [e. In: Callow [3] Meyer ency suppre, Plant //webbook.n

pounds extra55–360.

Co

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al profile as and Pad

Joana Costa,

atório de Faorto, R. Jorge

search on mnatural com

sting propertien reported iealth point o

es, like antioxbe classifie

own algae (Pthat are im

ance [2]. In th and Padinartugal were ds were idene anti-allergi2H3 cells d

nary data poimean ± SEMn in 40.0 ± 4.5

spite of F. snti-allergic ac(C18:3) and

olic composit

ressed as mndex obtaine

nts: This wo/EQB/LA0002/2009).

Andrade et a[2] Amsler e

w JA, editor. Aet al. (200

sses ArabidPhysiol

ist.gov/chemacted from b

ompounds Proline

loroglucinol C14:0

Mannitol C16:0 C18:3 C18:1 C18:0 C20:4 C20:5

Fucosterol

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and anti-adina pavon

Tiago Pereira,

armacognosia,Viterbo Ferrei

marine produmpounds bees. In fact, mn macroalga

of view, both xidant, antitued into threePhaeophyta)portant memhis work, etha pavonica (chemically c

ntified by GCc screening

degranulationnt P. pavoni) the cells d59%, for the spiralis to bectivity, whichof phlorogluc

tion of F. spir

mean (SD) ofed in the exp

ork was supp6/2011). B.

al. (2013) Valet al. (2005) Advances in09) Remodeopsis germin

151:603mistry/name-sbuds of Popu

RIcalcb

1286 121631 161837 181896 192043 202225 222238 222253 222389 232398 233361 33

p.edu.pt/bio

llergic scrnica (Linna

, Patrícia Vale

, Departameira n.º 228, 40

ucts has shoelonging to more than 15ae and differ primary and

umor and ante classes ba) and red algmbers of mhanolic extrac(Linnaeus) Tcharacterized

C–MS (Table g was assesn by non-toica as the modegranulation

same concee more chemh could be recinol.

iralis and P. p

f three deterperiment. c RI

ported by F. R. Pinho

luable compDefensive a

n Botanical Reled respiratnation and g3–619. ser.html). [5]ulus balsam

RIlitc 299 [3] 3647 [4] 843 [4] 6912 [4] 12040 [4] 6218 [4] 248 [4] 216234 [5] 2373 [4] 4380 [4] 2330 [5] 3

plant 

reening ofaeus) Thiv

então, Paula B

nto de Quím050-313 Porto,

own that seadifferent m

5000 primary rent applicatid secondary ti-inflammatoased on theae (Rhodophany marine cts of two broThivy], collecd and their a1), F. spirali

ssed by inhoxic concentost promisin

n at 12.5 mgentration. mically rich telated, at lea

pavonica sam

rminations; LIlit: Retention

Fundação pao is indebt

ounds in maand SensoryResearch, Votion in ndufgrowth and a[4] NIST] Isidorov et ifera and Po

F. spiralis 39.68 (9.68)

< LOQ 6.98 (0.27) 27.38 (3.48)

60.60 (2.06) -

69.06 (166.76)20.84 (2.14) 46.56 (2.90) 21.70 (1.14) 31.76 (0.94)

          4ª Work

f Fucus spvy

B. Andrade, Br

mica, Faculd, Portugal.

weeds are aetabolic patand secondons are assmetabolites

ory [1]. eir pigmentahyta). There

communitieown seaweected in differanti-allergic pis presentinghibition of ctrations of tg species, b/mL, while F

han P. pavoast partially, w

mples (mg/10

LOQ – limit o index descr

ara a Ciêncited to FCT

croalgae exty Chemical Eolume 43: Acfs4 with lowalters controlT chemial. (2003) G

opulus nigra

P. pavon13.48 (2.21.02 (0.

- 38.32 (1.12.16 (3.

186.02 (2) 146.92 (4

14.04 (2.- -

16.48 (0.

                     kshop Anual Bi18 / 19 Julh

piralis

rígida R. Pinho

dade de Far

a rich sourcethways, andary metaboli

signed to the exhibit num

ation: green is a wide div

es with signeds species [Frent places properties stg a great varcalcium ionothe two eth

because it redF. spiralis red

onica, the laswith the pre

00 g dry alga

of quantificaribed in litera

ia e a TecnoT for the

tracts, Food Ecology of Bcademic Pre

w phosphoryl of metabolistry we

GC–MS analy, Z Naturfor

nica 78) 06)

58) 38)

2.20) 4.88) 42)

56)

                     

29 

ioplant o 2013 

o

rmácia,

e of a d with ites

em [1]. merous

algae versity ificant Fucus of the udied. iety of

ophore anolic duced duced

st one sence

ae)a

tion. b ature.

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P16

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6 Carga oraustralis

Manuel Oliveir

artamento de tro de Física, tro de Investig

Estações de nica e aumentoETARs uti

etudo adequunidades. É a leitos planceae cosmope período danecem ime

s de FitoETAratório ensaiocolmos de ciderável teora orgânica dnui, enquantbilizar ao fim

7 Halotole

sa, J., Gonçalv

EUP – CIGARersidade do PoCUP – DepartaCampo AlegreUM - Departagal IIMAR – Cen

Bragas 289, 40

tolerant and city to withsme conditionty and on m

ering practicaa latifolia wtation to thendent on sa

es in late sphermore, it wite some grots. The best th and surviv. Furthermora recirculatito uptake d

unts of salts sher studies, ediation of sa

 

                      

rgânica ads mantidos

ra1, António M

Biologia, EscoEscola de Ciê

gação em Tec

Tratamentontar a qualidlizam leitos duadas para já considerá

ntados com polita seca, edo ano, frio ersos durantARs pelo enos em microcaniços imerr de carga oda “água da to que o pde algumas

erant Plant

vesb, Ana T., M

R - Centro de Iorto, Rua Dr. Ramento de Bio

e s/n, 4169-00amento de Bi

tro Interdiscip050-123 Porto

halophytic stand high sans. This studmethods to al informationwas collectee tested subsampling seasring (11%),

was able to owth inhibitio

results wereval. With proe, Typha lating aquacultu

dissolved sasome salinitytherefore, w

aline environm

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dicionada s nos leito

Mário Almeida2

ola de Ciênciaências da Univcnologia Agro-A

de Águas ade sanitáriade plantas pa

o tratamenável o númePhragmites

e os seus coe geralmen

te largos pecharcamentocosmos.Os rsos em águ

orgânica na “chuva” estim

H e a tenssemanas.

ts for Phy

Minac, Isabel, B

Investigação eRoberto Frias,ologia, Faculd7 Porto, Portuiologia - Univ

plinar de Inves, Portugal.

plants havealinity whichy focus on Tsuccessfully n for in situ red and propstrate, expanson, with higprobably duwithstand s

n. Arundo doe obtained inper acclimatifolia was alsure farm. Holts. Althougy reduction cwill be aimedments.

p.edu.pt/bio

a FitoETAos durante2 e Isabel A-P.

as da Universiversidade do M-Ambiental e C

Residuais (a das águas ara o tratamnto de águ

ero de FitoETaustralis (c

olmos são, ente caractereríodos.Parao causado pensaios prel

ua da torneir“água da chmada pela Csão superfic

ytoremedia

Borgesb,d, Ma

em Geo-Ambi, s/n 4200-465

dade de Ciênciugal. versidade do

stigação Mari

e been the h opens up nTypha latifolia propagate

remediation apagated withnded clay, a

gher values ie to a lower

salinity valueonax was con the spring.tion, survival so able to wiowever, it regh expandedcannot be exd at clarifyi

plant 

AR por coe o outono

Mina1, 3

idade do MinhMinho (CF-ECCiências Biológ

ETAR) têm afluentes. ento secund

uas residuaiTARs existecaniço). Entrm muitas Fitrizado por fa estimar a pelas chuvasliminares atéra durante uhuva experimCarência Bio

cial aumenta

ation in Sa

ria-Teresa,

iente e Recurs5 Porto, Portugias da Univers

Minho, Camp

inha e Ambien

subject of inew opportua and Arundand acclima

applications h intact rhizafter 2 weeksn autumn (7r carbohydraes of up to llected and p Expanded crate was higthstand and

emains uncled clay itself plained by sng the pote

          4ª Work

lmos de Po/inverno

ho (DB-ECUM)CUM) gicas (CITAB)

por objetivo

ário de águais domésticantes em Pore setembro toETARs, maortes aguaccarga orgâns invernais, é agora realizma semana

mental”. Ao looquímica de a, mostrando

aline Envi

sos, Faculdadgal sidade do Port

pus de Gualt

ntal, Universid

ntense reseunities for phdo donax abilatize these slike saline efzomes. It res. However, 75 to 90% suate concentra2.4% with 9propagated tclay utilizatiogh (=100%) utreat highly

ear if these pis able to

ubstrate adsential use of

                     kshop Anual Bi18 / 19 Julh

Phragmite

M)

)

o reduzir a

as residuais scas de peqrtugal e a me fevereiro

antidos nos ceiros, os cnica adicionarealizaram-szados evide, conduzem ongo do tem Oxigénio (Co tendência

ronments

de de Engenha

rto,

tar 4710-057

dade do Porto

earch due tohytoremediatlity to toleratespecies aimffluents treatevealed ade

survival rateurvival) and ation in rhizo94% survivathrough secoon increasedup to 3.5% ssaline waste

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30 

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es

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algae Fuc264.7

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Universitätstra

chemical dereme atmosificant diversw years. er the Portugacids consti

f compoundss. F. spiralis n fatty acidssyl diacylglyraction of thehy. Their struory activity o

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activity withpotential of

Tecnologia 09) is indeb

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us

Jorge

asse 1,

efence pheric sity of

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acetyl and buoassay. As ements wereidal alkaloidlentum systethose develo

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ciejewska, M. C

atório de Faorto, Rua de J

culentum Millalth impact.

ptera littoralisand the evaluive compouis materials (haracterized etabolites wenly in L. escentified in alcids seem to capacity of species (Figt, followed by

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l. (tomato plaNevertheles

s (Bois.). Thuation of thends, taking (larvae, pupa

in terms oere determinculentum leal matrices, ebe bioaccumthese matr

gure 1). They that of S. lit

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active matriceating the bioiscover the s insect to fe

cation would

hors are gratA0006/2011.

p.edu.pt/bio

teroidal alate-induce

ta, P. Valentão

, DepartameFerreira, nº. 22

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profit from ae, adults anf phenolics,

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especially in mulated by Sices was ch

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nto de Quím28, 4050-313

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, O2●- and ●N

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ação para a SFRH/BD/62

          4ª Work

rom Lycopy in SH-SY

de

mica, FaculdPorto, Portuga

mportant cropts that can deis pest justing in mind ition. The mets) and the hamino acidsGC-MS tech

S. littoralis mS. littoralis exrvae. hemical mictomato leav

NO. Values

evaluated usmato leaves e associatedmpounds in ant to overcoplant. Based

op plants mo

Ciência e a 2662/2009) i

                     kshop Anual Bi18 / 19 Julh

persicon Y5Y cells

dade de Fargal

p in the worldestroy its cuified the chets possible uetabolic prohost plant mas, fatty acidshniques. Phematerials. Ste

xcrements. A

croassays aves was the

show mean

sing a coloris and S. litd to the cont

the S. littorome the pesd on the obtore resistant

Tecnologia is indebted to

                     

32 

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rmácia,

d, with ltures, emical use as file of aterial s and

enolics eroidal Amino

gainst most

± SE

metric ttoralis tent in ralis/L. st, and tained to the

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QUIMTE/Labo, Portugal earch Group onology, CEBA

ative stress ders, such ylcholinesterawhile mono

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delia robustaengers and aPLC-DAD-ESntoside+apig

ucoside (Figenging capanst the otherk, but the aqugher content

 

re 1 - HPLCcts of Grindoylquinic acioylquinic acioylquinic aciucosyl-3´/4´-Ometin-7-O-gluyl-quercetin

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owledgemenrant no. PEED Program2011-23690

RH/BPD/6392

 

                      

a robusta on of its ine species

vedoa, F. Ferr

ratory of Pha

on Quality, SS (CSIC), P.O

is considereas Alzheim

ase (AChE) oamine oxidase last also pts the biologa Nutt. as ROas AChE, BuSI-MSn, bothgenin-7-O-glu. 1). Concecity than asr oxidative sueous extrac of hidroxyci

C-UV chromadelia robustaid; 4 – querd; 7 – 3,5-d; 10 – querO-pentoside;ucosyl-3´-O-p

glucoside; diosmetin-7-Oin pentoside3-methyl-ququercetagetimethylether;36 – 6-hydrinnamic acid

nts: The authEst-C/EQB/LAmme (Ref.

(CICYT). 22/2009).

    www.map

Nutt. : Chnhibitory p

reresb, A. Gil-I

armacognosy,

Safety and BioO. Box 164, 30

d to be one mer’s disea

and butyrylase A (MAOrevent the prical potentia

OS (O2●− an

uChE and MAh extracts beiucosyl-4´-O-rning the ancorbic acid,

species. Thect proved to nnamic acids

atogram (34a. 1 – 5-cacetin-3-O-hedi-feruloylqurcetin-7-O-gl; 13 – 4,5-dipentoside; 118 – 3-metO-glucoside;; 24 – luteoliercetin; 28 –n-3,6,3´/4'-tr34 – kaem

oxy-kaempfed 2; A3 – hyd

hors are gratA0006/2011,

112RT0460Clara Gro

p.edu.pt/bio

haracterizapotential a

Izquierdob, P.

Chemistry De

oactivity of Pl0100 Campus

of the causese (AD) ancholinesteraO-A) inhibitoroduction of

al of two extnd H2O2) andAO-A inhibitoing dominate-pentoside antioxidant acbut they we

e ability of thbe a good Ms in comparis

40 nm) of thaffeoylquinic exosyl-7-O-guinic acid; 8 ucoside; 11 i-caffeoylquin16 – apigenthyl-querceti; 21 – quercin; 25 – dios– dimethyl-qrimethylethermpferol-3-meerol-3,6,7/4´-droxycinnam

teful to Fund, to "Operaç0) CORNUCosso thanks

plant 

ation of thagainst br

Valentãoa, P.

epartment, Fa

lant Foods, DUniversity Es

es of the devnd depress

ase (BuChE)ors are useH2O2, a reacracts (aqueod reactive nors. The pheed by the paiand apigenctivity, the eere weaker the extracts tMAO-A inhibison with the

he aqueous acid; 2 –

lucoside; 5 –– 3,5-di-ca

– quercetin-nic acid; 14 nin-7-O-glucon glucoside

cetin; 22 – lumetin-3´-O-pquercetin per; 32 – quer

ethylether; 35-trimethylethe

mic acid 3; F1

ação para a ção NORTECOPIA Thes FCT fo

          4ª Work

he phenolirain enzym

B. Andradea

aculty of Phar

Department of pinardo, Murc

velopment oion. Moreovameliorate

ed to treat ttive oxygen s

ous and metitrogen specnolic profile wrs diosmetinin-7-O-gluco

extracts dispthan the refeto inhibit chotor, which cahydrometha

(A) and hyd3-feruloylqu– 5-feruloylq

affeoylquinic -3-O-hexosid– luteolin-7-osyl-4´-O-pe

isomer; 19uteolin-3´/4´-Opentoside; 26ntoside; 29

rcetin-3,3´-di5 – querceter; A1 – hyd1 – flavonoid

Ciência e a -07-0124-FEmatic Netwr the Pos

                     kshop Anual Bi18 / 19 Julh

ic profile ames and

rmacy, Univer

f Food Scienccia, Spain

of neuropsycver, inhibitothe sympto

the symptomspecies (ROthanol/watercies [RNS, (was charact

n-7-O-glucosyoside+diosmeplayed bettererence compolinesterasesan be attribu

anolic one.

dromethanolinic acid; 3

quinic acid; 6acid; 9 – 3

de; 12 – luteo-O-glucosideentoside; 17 9 – apigeninO-pentoside6 – apigenin– apigenin;

methylether;tagetin-3,6,7roxycinnami

d 1; F2 – flav

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33 

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and

rsity of

ce and

hiatric ors of ms of ms of

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●− pound s was

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vonoid

(FCT) 69", to project owship

                       

                     

1 Enzymes

eia P. Oliveirantão1, Paula B.

QUIMTE/Laborersidade do Poearch Group onology, CEBA

r betle L., aropical regionleaves act aheir medicixidant, antifuwork, aqueou

mical composdetermined E) and butyore active ag

nst AChE (ICty was also 1C).

. Enzymatic es: (A) AChEriments, perf

results suggeimer´s diseosidase, resp

rences: anguly, M. eajumdar, B. urata, K. et a

nowledgment no. PEst-C/

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a1, Joana Fer. Andrade1

ratório de Faorto, Rua de Jon Quality, SS (CSIC), P.O

a member ons of the wos breath-fresnal properti

ungic, antiheus lyophilizesition and bioby GC-MS.Brylcholinestegainst BuCh

C50 = 0.264 mevaluated, b

inhibitory aE, (B) BuChformed in trip

gest that P. ease and dpectively.

t al., 2007. Tet al., 2002. al., 2009. Jou

nts: The aut/EQB/LA000

2 3e] (mg/mL)

B

    www.map

on by Pipe

rreira1, Sofia

armacognosiaJorge Viterbo FSafety and BioO. Box 164, 30

of Piperaceaorld, recognizshener and aies, such almintic, xanthd and ethanological poteBoth extractserase (BuChEE (IC50 = 0.6

mg/mL) (Fig. being ethano

activity of aqhE and (C) plicate.

betle leavesdiabetes, du

The Journal oIndian Journurnal of Natu

hors are gra6/2011.

B

p.edu.pt/bio

er betle L.

Riboira1, Fed

a, DepartameFerreira, nº 22oactivity of Pl0100 Campus

ae, is a widzed as a tradare used in as anti-inflahine oxidaseolic extracts

ential. Aminos showed a gE). In a gen

601 mg/mL) w1A and 1B). olic extract t

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of Pharmacynal of Clinicaural Medicine

ateful to Fun

plant 

leaves

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dely distributditional herbthe Indian smmatory, a

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ted plant in al remedy foystem of me

anti-allergic, d chemoprevwere analyzacids, stero

al against ace aqueous ehanolic one wre, the α-gluceresting (IC5

ethanolic exshow mean

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acology, 59, try, 17, 49-57359.

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                     kshop Anual Bi18 / 19 Julh

l-Izquierdo2, P

dade de Faral f Food Scienccia, Spain

the tropicaor many diseedicine and

hepatoproteventive [1, 2,zed regardingols and tritercetylcholinestextract reveawas more effcosidase inh50 = 0.069 m

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belongs to Bally known as been assobetic and anfor authenticd in the locaty acids, steacterized by vertheless, dd a potent don the enzyotoxic to botrcinoma cell).

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oraginaceaeas scramblinociated with tioxidant proity control, al market, from

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ra diffusa (Lagromwell, thntihypertensivmed the use

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Biology R.M.T.,

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