4TH ANNUALADVISORY PANEL

MEEETING

10 January 2013San Diego, California

Outline of Presentation

RosBREED

Project Overview

Morning Session

Afternoon Session

Project Overview

RosBREED

RosBREED Mission Statement

We will develop and apply marker-assisted breeding, based on improved knowledge of industry value and consumer preferences, to accelerate and increase

the efficiency of rosaceous cultivar release and successful cultivar adoption

4 yrs$7.2M federal$7.2M matching

Amy Iezzoni, Michigan State Univ.Cameron Peace, Wash. State Univ.

This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

RosBREED Will Bridge the Chasm

Genomics Resources

Marker- assisted breeding

Genomics

knowledge

More efficient development of new

cultivars

RosBREED’s Focus is on Fruit Quality

RosBREED Will Develop Tools

$Marker-assisted

breeding in application

For those traits that have value for producers, market intermediaries and consumers

RosBREED Will Use Improved Knowledge

Courtesy David Byrne, Texas A&M Univ.)

• of industry values and consumer preferences to select target traits for Marker-Assisted Breeding

• Impacts: increase new cultivar adoptionenlarge market potential

Supporting Breeding Decisions

WA2

Which traits to target? Which parents to use?

Which combinations to create?

Which seedlings to progress?

Which selections to trial?

Which advanced selections to commercialize?

WA2

Which wild germplasm to use?

Which traits to target? Which parents & combinations to create?

Which seedlings to progress?

Which selections to trial?

Which advanced selections to commercialize?

Supporting Breeding Decisions

RosBREED

Long-term outcomesFour year project outcomes

Increased gain in fruit quality per breeding

cycle due to improved parent selection and

improved mean progeny value

More rapid availability of new

cultivars with genetically superior

fruit quality

Improved profitability and sustainability of US rosaceous fruit, nut, and

floral crops with increased

consumption and enjoyment

Implementation of MAB by breeding

programs

RosBREED’s Target Outcomes

Project AssistantsAudrey SeboltJoan Schneider

Extension Team Leader: Cholani Weebadde

Genomics Team Leader: Dorrie Main

Genotyping Team Leader: Nahla Bassil

BIMS Team Leader: Gennaro Fazio

Socio- Economics Team

Leader: Chengyan Yue

MAB Pipeline Team Leader

Cameron Peace

Pedigree- Based Analysis Team Leader: Eric van de Weg

Breeding Team Leader

Jim Luby

Apple· Jim Luby· Susan Brown· Kate Evans

Strawberry· Chad Finn· Jim Hancock· Tom Davis

Peach· Ksenija Gasic· John Clark· Dave Byrne· Tom Gradziel

Cherry· Amy Iezzoni· Nnadozie Oraguzie

Executive CommitteeNahla BassilMichael CoeGennaro FazioKarina GallardoKsenija GasicJim Luby Dorrie MainJim McFersonCameron Peace Eric van de WegCholani WeebaddeChengyan Yue

Project DirectorAmy Iezzoni

RosBREED’s Management

Demonstration Breeders

&

Breeding Trainees

CookHarrisonvan Sickle

HirstWatkins

Karp

AbbottBuellGardinerHummer

ChengSimon

Ross

Industry

Extension

Scientific

Allen GoldbergerCarr OlmsteadDodd ShermanGrim ArusKorson AvariLott

BierlinkCurtisPerrySparksSeavertBliss

Baugher Stokes

Moffitt

Advisory Panel Members

Plant Research International, NetherlandsEast Malling Research, UKCRA-FRU, Rome, ItalyIASMA, Trento, ItalyINRA (Bordeaux, Avignon, Angers), France

Andres Bello University, Chile Plant & Food Research, New Zealand

ARC, South Africa

RosBREED participant

location

International Collaborators

+ 2 Scientific Advisory Panel members (Spain, New Zealand)

Morning SessionGoal = provide information to AP members

to set the stage for receiving advice

RosBREED

Start End Audience/ Group

Presenter/moderator Title

8:00 8:30 All Amy Iezzoni Introductions

8:30 9:00 All Amy Iezzoni Overview of impact

9:00 10:00 All

Karina Gallardo Dorrie MainNahla BassilEric van de WegGennaro Fazio, Jim Luby

Target traits, Genome Scans, Pedigree-based breeding analysis, Toolbox and breeding (Obj. 1-3): Goals, Deliverables, Challenges, Year 4 Deliverables, and Q & A

10:00 10:30 Break (BIM System computer demonstration available)

10:30 11:00 All Cameron PeaceCholani Weebadde

MAB pipeline and extension (Obj. 4-5) Goals, Deliverables, Challenges, Year 4 Deliverables, and Q & A

11:00 11:15 All Michael Coe Evaluation of Extension efforts

11:15 11:45 All Amy/Cameron Plans for RosBREED #2

11:45 1:00 Lunch

Morning Session

Major Enabling Deliverables

• Enabling computer-based accomplishments that allow us to find, polish and put the Jewels into application

• Computer demonstrations at breaks:

Eric van de Weg Pedigree-Based

Analysis software

Dorrie Main BIM System

Cholani WeebaddeJewels in the Genome in eXtension

External Evaluation of Extension Efforts

Dr. Michael CoeCedar Lake Research Group, LLC

From Natural Diversity to Better Cultivars

Afternoon SessionGoal = receive advice from AP members

RosBREED

1:00 1:30 All Karina GallardoJim Luby Demo of apple auction experiment

1:00 3:00 Group 1

Discussion Session Group 2

Ideas and approaches for addressing industry opportunities, needs and limitations

Ideas and approaches for addressing breeding opportunities, needs and limitations

3:00 3:30 Break (BIM System computer demonstration available)

3:30 4:15 All DS 1 & 2 moderators Reports and Discussion – possible next steps

4:15 5:00 All Cameron Peace Round table

5:00 All Dinner on your own or in working groups

Afternoon SessionStart End Audience/

GroupPresenter/moderator

Title

Group 1: Remain in the ballroom

Group 2: Move to Cortez room

RosBREED

Discussion Session

1. What methods or plans of work could be done differently to better meet RosBREED’s current industry and breeding project goals? 

2. What unanticipated opportunities, needs and limitations should we address within the current project?

3. Thinking ahead to a second project, how well do our proposed objectives effectively address current and future industry and breeding opportunities, needs and limitations? What modifications would you recommend?

1:30 3:00 Discussion session

GROUP 1: Remain in Ballroom Ideas and approaches for addressing industry opportunities, needs and limitations

GROUP 2: Cortez Room Ideas and approaches for addressing breeding opportunities, needs and limitations

3:00 3:30 Break (BIMS computer demonstration available)

3:30 4:15 All DS 1 & 2 moderators Reports and Discussion – possible next steps

Jewels in the Genome Analogy

Find

An individual’s genome is the full complement of genetic information that it inherited from its parents.

Within this vast repertoire of genetic information, individuals genes are being discovered that control critical production and fruit quality traits.

Put into application in breeding

Jewels= valuable genetic discoveries ready for breeding application= marker-locus-trait associations validated in a breeding program

Polish

RosBREED seeks to find, polish and put into application predictive DNA tests for valuable Jewels in the Genome

“Jewels in the Genome” Available Now!

The Jewels are Linked on Chromosomes

56 chromosomes in strawberry!!

Acknowledgements

This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

MSUAmy Iezzoni (PD)

Jim HancockDechun Wang

Cholani Weebadde

WSUCameron Peace

Dorrie MainKate Evans

Karina Gallardo Vicki McCracken

Nnadozie OraguzieFormer WSU

Raymond JussaumeMykel Taylor

CornellSusan BrownKenong Xu

Clemson Ksenija Gasic

Gregory Reighard

Texas A&MDave Byrne

Univ. of CA-DavisTom Gradziel

Carlos Crisosto 

Univ. of New Hamp.Tom Davis

Univ. of MinnesotaJim Luby

Chengyan Yue

Oregon State Univ.Alexandra Stone

Plant Research Intl, Netherlands

Eric van de WegMarco Bink

USDA-ARSNahla Bassil

Gennaro FazioChad Finn

Univ. of ArkansasJohn Clark