3 ways to obliterate bullet points from your slides - slide makeover 2

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a w esome presen t a tions newsletter slide make o ver 2

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3 Ways to Obliterate Bullet Points From Your Slides

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Page 1: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

awesome presentations newsletter !

slide makeover 2

Page 2: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

My#Role#on#the#Project#

/  Tes2ng#an2bodies#/  Determining#sensi2vity#of#IRIS#for#pseudotyped#VSV#detec2on#/  Determining#repeatability#/  Valida2ng#new#IRIS#instruments#(IRIS#on#med#campus)#/  Standardizing#the#chip#prepara2on/incuba2on#protocol##/  To#improve#sensi2vity#and#repeatability#with#surface#op2miza2on#

Page 3: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Hi, my name is Collaborator X and I'm responsible for optimizing the protocol that we use to coat the chips with antibodies. My job is to make sure that each chip has as few foreign particles as possible, and that each antibody has a high affinity for the virus we are trying to detect. After incubating the chips, I scan them and analyze the data. Then, I try to come up with ways to lower the variability and improve the sensitivity of our results.

Page 5: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Chip%Prepara*on%Protocol%

Chip%Spo(ng%&%Washing:%%0%An*body%solu*ons%are%spo7ed%at%305%mg/ml%in%PBS%with%50%mM%Trehalose.%%%%%0%ADer%overnight%incuba*on%in%the%spo7er%(at%60%%humidity),%chips%are%washed%in%PBST%for%30%minutes,%rinsed%in%1X%PBS%for%3%minutes%and%dI%for%3%minutes%and%dried%with%argon.%

Incuba4on%of%Chips%with%Concentrated%Samples:%!0%Chips%are%scanned%on%IRIS%for%pre0incuba*on%par*cle%counts.%%%0%Chips%are%incubated%with%1%ml%of%the%virus%sample%for%2%hours%on%shaker.%%

0%ADer%incuba*on%ends,%chips%are%washed%in%1X%PBS%for%3%*mes,%each%1%min%and%then%0.1X%PBS%for%1%min,%dipped%in%dI%for%10%seconds%and%pulled%out%of%the%water%slowly%to%let%the%water%slide%down%the%chip.%%%0%Chips%are%scanned%with%IRIS%for%analysis.%

Page 6: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Spotting and Washing Pre-incubation scan

Incubation with virus Post-incubation scan

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LED

LED

Page 7: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Future&Work&

*  Dilu.on&experiment&for&Marburg*pseudotyped&VSV&

*  10^4&PFU/ml&detec.on&in&30&minutes&

&

*  Tes.ng&of&New&Lassa&An.bodies&(7&of&them)&&&

*  Dilu.on&curve&for&Lassa*pseudotyped&VSV&for&LOD&determina.on&

Page 8: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Obtain dilution curves for!Marburg- and Lassa-pseudotype VSV

Page 9: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Obtain dilution curves for!Marburg- and Lassa-pseudotype VSV

Try to detect viruses at lower !concentrations (104 PFU/ml) after !shorter incubation periods (30 minutes)

Page 10: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Obtain dilution curves for!Marburg- and Lassa-pseudotype VSV

Try to detect viruses at lower !concentrations (104 PFU/ml) after !shorter incubation periods (30 minutes)

Test 7 new Lassa antibodies

Page 11: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2
Page 12: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2
Page 13: 3 Ways to Obliterate Bullet Points From Your Slides - Slide Makeover 2

Click on the envelope to get an email with

the next slide makeover

(probably on sunday)