25.4.2006 distributor meeting landshut 2006 new products sera cell culture media - classical special...
TRANSCRIPT
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS
Sera
Cell Culture Media - Classical
Special Media
Reagents
Growths Supplements
Transfection Reagents
Cell Preservation
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SERA
FBS – New Origins
EU approved: Argentina, Brasil
USA origin: North America
AUSTRALIEN origin: Australia
CANADIAN origin: Canada
USDA approved: Central America - Mexico
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SERA
FBS, PAA Clone
very low Endotoxin = under 1 EU / ml
various origins
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SERA
FBS Pre-Tested
FBS ES (Embryonic Stem Cells) tested
supports the growth of undifferentiated colonies of ES cells
recommended for most primary cells
for low unspecific stimulation
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SERA
FBS Pre-Tested
FBS Amnion Cell tested
cultivating of amnion and chorionic villi cells
cultivation of lymphocytes
pre and post –natal diagnostics
Karyotyping
FISH - analysis
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SERA
FBS Pharma Grade
= formally known as „FBS EMEA tested“
origins: USA and AUSTRALIA
standard and gamma irradiated
BVD antibody negative
Tested according to: Ph. Eur. 5.4 monograph 4/2006:2262
the EU regulation 2005/567 AT
CPMP/BWP 1793/02
CVMP/ 743/00
9CFRPH
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SERA
FBS Neo-Natal
collected from calves less than 12 hours old
prior to ingestions of colostrums
economical alternative to FBS
USDA approved
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS CELL CULTURE MEDIA
DMEM
with Stable Glutamine
without Phenol Red
with D-Valine, without L-Valine, +/- L-Glutamine
without Calcium
without Glucose
thermostable, without L-Glutamine
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS CELL CULTURE MEDIA
RPMI 1640
with Stable Glutamine
Thermostable without L-Glutamine
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SPECIAL MEDIA - Neuronal
Neuronal Base Medium
Serum free cell culture of neuronal cells
For reduction of glial cells
Optimal growth of embryonic neurons
For proliferation of neuronal stem cells
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SPECIAL MEDIA - Neuronal
Neuronal Base Medium AD
AD = adult neurons
Serum free
Long term cultivation of postnatal rat hippocampal
and other adult mammalian neurons
Long term cultivation of different adult neurons from the CNS and PNS
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS Growths Supplements - Neuronal
B27 NeuroMix
G5 Supplement
N2 Supplement
Neuronal Stem Cell Supplement
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SPECIAL MEDIA – Stem Cell Media
CollagenStem Kit = Medium & Solution
Detection and quantification of hematopoietic progenitors
Formation and isolation of distinct colonies
Cytochemical or immuno-cytochemical staining
Serum free basal medium
Good optical clarity
Easy visualisation
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS SPECIAL MEDIA – Stem Cell Media
MethoStem Medium
Growth and differentiation of hematopoietic stem cells
Contains methylcellulose
Differentiation and characterization of stem cell progenitors
For further cultivation and separation of single cells by colony selection
Growth of embryonic stem cells
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS REAGENTS
Stable L-Glutamine (L-Alanyl-L-Glutamine) No toxic ammonium ions build up due to degratation processes
Incubating: 37°C
Storage: 15°C - 25°C
Direct substitute for L-glutamine = stable dipeptide form
For mammalian cell cultures
For suspension and adherent cell cultures
Essential media component
C
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS REAGENTS
ALFAZYME contains a mixture of strong negative charged particles
+ mild acting enzymes
for sensible cell lines
more gentle
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS TRANSFECTION REAGENTS
Nanofectin Kit (Nanofectin & Diluent)
Transfection reagent for mammalian cells
consists of a positively charged polymer and porous nanobeads
efficient binding of DNA
protects bounded DNA against degradation
high Transfection Rate
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS CELL PRESERVATION
CoolStar
Optimized medium for hypothermic preservation (2°C to 8°C)
Preservation of different mammalian cells, tissues and organs
Gentle cold storage
Transportation of cells
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS CELL PRESERVATION
CoolStar Kit = CoolStar &
CoolStar Rinse
flush solution
to rinse culture media and native fluids from mamma lian cells, tissues and
organs in combination with CoolStar
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25.4.2006 Distributor Meeting Landshut 2006
NEW PRODUCTS CELL PRESERVATION
CryoMaxx
Optimized cryopreservation (liquid nitrogen)
minimized dehydration effects
also for serum containing media
for a wide variety of cell types
for cell banking
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25.4.2006 Distributor Meeting Landshut 2006
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS
CYTOGENETICS IS….
the study of normal and abnormal CHROMOSOMES
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
A variety of techniques to determine the health and condition of an unborn fetus
by analysis of:
Amnion Fluid (>14th week)Chorionic Villi Cells (>10th week)
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
INDICATORS
maternal age – more than 35 years earlier abortion parental chromosomal aberration abnormalities at ultrasonography mutagene exposure
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS POSTNATAL
Diagnosis after birth and later…
by cultivation of T-Lymphocytes (3-4 days) out of 5ml whole blood
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS POSTNATAL
IndicatorsStrange phenotypeEnzymatic disorderLater: strange growth behaviour of childrenSterility problems
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS
Numerical Aberation
Trisomy 21 – Down Syndrome
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICSA list of diseases based on numerical abarations
Adrenogenitales Syndrom (AGS) Alpha-1 Antitrypsin-Mangel Anenzephalie Angelman-Syndrom APC-Resistenz Autismus Bluterkrankheit Chorea Huntington Creutzfeldt-Jakob Disease Cystische Fibrose Dandy-Walker-Fehlbildung Down-Syndrom Dysbetalipoproteinämie Edwards Syndrom Eisenspeicherkrankheit Faktor-V-Leiden Fatal familial insomnia Fischschuppen-krankheit Fragiles-X-Syndrom Gaucher-Syndrom Gerstmann-Sträussler-S. Syndrom Hämochromatose Homocystinurie Huntington-Krankheit Hutchinson-Gilford-Progerie-Syndrom Hypercholesterinämie Hyperlipidämie Typ III Ichthyose
Hyperlipidämie Typ III Isolierte Lissenzephalie-Sequenz Katzenschrei-Syndrom Lesch-Nyhan-Syndrom Lissenzephalie-Sequenz, isolierte Martin-Bell-Syndrom Miller-Dieker-Syndrom Morbus Fabry Morbus Gaucher Morbus Osler Mukoviszidose Muskeldystrophie Neurofibromatose Noonan-Syndrom Osteoporose Pätau Syndrom Phenylketonurie (PKU) Prader-Willi-Syndrom Progerie Typ I Progerie Typ II Prothrombin-Mutation Recklinghausen-Krankheit Retinoblastom Salzverlustsyndrom Trisomie 13 Trisomie 18 Ullrich-Turner-Syndrom
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS
STRUCTUAL ABARATIONS
17p13.3 – Miller Dieker Syndrom
Loss of chromosomalMaterial = DELETION
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
Quantum 3-21
Complete medium for prenatal diagnostics
No supplementation necessary
Selective cultivation of amniotic fluid cells and chorinonic villi cells
Ready to use medium with FBS, growth factors, hormones and
optimised base medium
For use in karyotyping
Tested in independent clinical laboratories
CE - certified
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
Ready to use media
Rapid growth
No chromosomal breakages
Simple detection of chromosome aberrations
Quick results
Cell harvest after 7-10 days
For clear diagnostic findings
Unlimited availability
Quantum 3-21
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
Who needs Quantum 3-21
Chromosomal Analysis
Institutes for Human Biology
State and Private Diagnostic Laboratories
Research in Human Genetics
Child Welfare Clinics
Clinical Research Labs
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
Competition Quantum 3-21
AmnioMax, Invitrogen (USA, market leader)
Amnio Chrome,Cambrex (USA)
Chang Medium, Irvine (USA)
BioAMF1/2, Biological ind. (Israel)
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS POSTNATAL
Complete medium for postnatal diagnostics
Optimised media for T- Lymphocytes
No supplementation necessary
Media with pretested FBS
Pre-tested and optimal adjusted PHA
CE – certified product
Quality control in independent cytogenetic laboratories
Quantum PBL
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
Who needs Quantum PBL
Institutes for Human Biology
Diagnostic laboratories
Clinical diagnostics
Research laboratories working with lymphocytes
Clinical research labs
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS PRENATAL
Competition Quantum PBL
KaryoMax, Invitrogen (USA, market leader)
LymphoChrome, Cambrex (USA)
Complete product line, Biological ind. (Israel)
Home made Media on base of Ham´s F12 + 20% FBS
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS
CE - Certification
CE certified product from a supplier
Nearly the same standard like cGMP
Declaration of conformity for CE marked products
Products must be produced in conformity to „Directive 98/79/EC“
Aim: constant quality (important for human diagnostics)traceability of the product must be
guaranteedAudit by legal authorisation is possible
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25.4.2006 Distributor Meeting Landshut 2006
CYTOGENETICS MARKETING
Explaining features and benefits of Quantum media
Offering free samples
Offering the Cytogenetic brochure
Samples will processed by distributor
Quotes will be done from distributor