2010 outbreak of vibriosis linked to chesapeake bay oysters with a novel … · 2017-06-14 ·...
TRANSCRIPT
![Page 1: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/1.jpg)
2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel Strain
of Vibrio parahaemolyticus
Julie HaendigesMaryland Department of Health – Laboratories Administration
![Page 2: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/2.jpg)
Chesapeake Bay - Overview
Images provided by Google Image
![Page 3: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/3.jpg)
Oyster Industry in Maryland
• Oysters are among the most valuable fisheries in the Chesapeake Bay ($15.7 Million in 2013-2014)
• The oyster population is rebounding after a major decline and increasing this population is a high priority in Maryland
• An oyster can filter 50 gallons of water in a single day
• Aquaculture is one of the key’s to the restoration. Oysters grow faster, mortality rate lower than bottom cultured oysters.
2014 – 14,693 Summer Harvest bushels
2015 – 23,768 Summer Harvest bushels
2016 – 27,999 Summer Harvest bushels
![Page 4: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/4.jpg)
Vibriosis Cases in Maryland 2010 - 2016
0
5
10
15
20
25
30
35
40
45
50
2007 2008 2009 2010 2011 2012 2013 2014 2015 2016
V. parahemolyticus Other Vibrio spp.
![Page 5: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/5.jpg)
Vibrio parahaemolyticus
• Leading cause of bacterial infections associated with consumption of seafood.
• Most people become infected by eating raw oysters, but infections can occur in wounds exposed to salt water.
• Estimate of 45,000 illnesses each year in the US• Bloodstream infections can occur and in most cases are fatal.
• Hot sauce, Lemon juice, and drinking alcohol does not prevent Vibrio infections!
www.cdc.gov/vibrio
![Page 6: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/6.jpg)
Vibrio Surveillance by Whole Genome Sequencing
• MD DHMH started WGS in 2012 with the Ion Torrent. Currently we have 4 MiSeqs and 2 Ion Torrent
• GenomeTrakr participant
• Collaborated with FDA-CFSAN on sequencing Vibrio parahaemolyticus
• Currently, we have sequenced 121 Vibrio sp. isolates
![Page 7: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/7.jpg)
2010 Outbreak of Vibrio parahaemolyticusRestaurant A
Restaurant B
![Page 8: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/8.jpg)
2010 Outbreak of Vibrio parahaemolyticusRestaurant A
Restaurant B
MD DHMH Lab
PFGE-SfiI PFGE-NotI
![Page 9: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/9.jpg)
Outbreak Investigation
• Invoices and Oyster Tags were collected from the restaurants
• A single source for the oysters was identified
• The harvest site was inspected and oysters were collected for further testing
![Page 10: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/10.jpg)
2010 – Investigation Continued
• With the help from Dauphin Island FDA Lab - 479 strains of V. parahaemolyticus were isolated from the oysters collected.
• 9 strains were considered potentially pathogenic based on toxin profiles
• PFGE was done on the recovered isolates.
In 2010, this is as far as we could go for characterization of these
isolates
![Page 11: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/11.jpg)
2012 – Whole Genome Sequencing!
• Rarely causative strains of V. parahaemolyticus are isolated from food sources.
• WGS provides us with new insights in Outbreak investigations
• Questions – How related are all of these strains? Where did this Outbreak strain originate?
Photo courtesy of Walter Calahan
![Page 12: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/12.jpg)
wgMLST Analysis
![Page 13: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/13.jpg)
wgMLST Analysis
![Page 14: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/14.jpg)
How did this strain get here?
![Page 15: 2010 Outbreak of Vibriosis linked to Chesapeake Bay Oysters with a Novel … · 2017-06-14 · Chesapeake Bay Oysters with a Novel Strain of Vibrio parahaemolyticus Julie Haendiges](https://reader034.vdocuments.us/reader034/viewer/2022050508/5f994f686790e7607112205a/html5/thumbnails/15.jpg)
Thank You!
• Molecular Epi Staff• Dr. Clifford Mitchell, MD DHMH• Alvina Chu, MD DHMH• Dr. Robert Myers, MD DHMH• Narjol Gonzalez-Escalona, FDA• Eric Brown, FDA• Marc Allard, FDA