2 members of the family enterobacteriaceae

7/23/2019 2 Members of the Family Enterobacteriaceae

1/27

MEMBERS OF THE FAMILY

ENTEROBACTERIACEAE


2/27

Citrobacter

Resembles Salmonella but are ONPG (+)& LDC (-)

Citrobacter speciesfreundii(!"# pneumonia# intraabdominal

abscess)

di$ersus(neonatal menin%itis)

amalonaticus(etraintestinal infections)

C' freundiiS' di$ersusS+-

"ndole-+

*CN+-


3/27

Klebsiella

Klebsiella -Enterobacter-Serratia-HafniaK. pneumoniae

,riedlanders bacilli (encapsulated andmucoid)

(+) Strin% test

Klebsiella aerogenes is associated with hospitalacquired infections of woundsand of t.e urinarytract

K. rhinoschleromatis/ r.inoscleromatis(%ranulomatous disease of t.e nose and p.ar0n

1it. intense s1ellin% and malformation of t.e entireface and nec2)


4/27

The patient'sK. pneumoniaeisolate

was string test positive (>5 mm

string length).


5/27

LABORAOR! "#A$%OSES

Specimens: Depending on the site of infection, specimens

include urine# pus# sputum and infected tissue'

&orpholo'y *lebsiellae are Gram ne%ati$e# non-motile# usuall0

capsulated rods'

(ulture

*lebsiellae are aerobes and facultati$e anaerobes'

Blood agar: Klebsiellae produce large grey white usuall0 mucoidcolonies'

MacConkey agar and CLED medium: Most 2lebsiellae are lactose-

fermentin%# producin% mucoid pin2 colonies on 3acCon2e0 a%ar and

0ello1 mucoid colonies on CL4D medium

K. rhinoscleromatis is non-lactose fermentin%' Biochemical tests

*lebsiellae are indole ne%ati$e# ornit.ine decarbo0lase ne%ati$e and do

not produce S'


6/27

Klebsiella on Macconkeymedium show largemucoid pink lactosefermenting colony

Klebsiella on CLED medium show

ellow la!tose "ermenting !olon


7/27

Enterobacter

LDC5DODCrease6ello1pi%ment

4' cloacae-++ 7-

4' aero%enes+-+ --4' %er%o$iae+-+ +-4' sa2a8a2ii-++ -+

4' ta0lorae-++ --

#spe!ies$ habitat$ soil% water% dair produ!ts

#normal "lora o" the gut o" animals &

humans

#motile% DC & * (+)


8/27

Enterobacter


9/27

Edwardsiella

4d1ardsiellatarda (.uman pat.o%en /

bacteremia and 1ound infection).os.inae (sna2es# birds and

1ater)

ictaluri (enteric septicemia infis.)


10/27

Serratia

opportunistic pat.o%ens

DN5se# lipase# %elatinase

9S' marcescens9S' li:uefaciens

9S' rubidaea

9S' oderifera


11/27

Serratia


12/27

Proteus

Tow important species , P. mirabilis and P.

vulgaris

&orpholo'y Proteus is actively motile with peritricous

flagella , non capsulate, Gram ne%ati$e

pleomorp.ic rods'

Rapid urease producers S1armin%# burned c.ocolate odor


13/27

Proteus with peritricousfagella


14/27

)atho'enicity

P. mirabilis causes:;- *rinary infections' in the elderl0 and

0oun% males and often follo1in%

cat.eteri8ation or c0stoscop0''

- Abdominal and wound infections


15/27

(ultureaerobically most Proteus cultures have a characteristic fishy Odour

and S1armin% on t.e medium

MacConkey CLED !LD media: non-lactose fermentin%colonies after o$erni%.t incubation at /


16/27

Proteus

P' mirabilis P' $ul%aris

"ndole-+

ODC+-

,ermentation 3altose - +

A0lose + + Salicin - +

C.loramp.enicolSR


17/27

Proteus

P' mirabilis P' $ul%aris

"ndoleBBB BB'

3altoseBBBBB'

A0loseBB''BB'

SalicinBB''BB'

C.loramp.enicolBB''BB


18/27

Proteus

)roteus3cCon2e0 rease test


19/27

Swarming on Blood

agar,warming pehomenon


20/27

Morganella

,ormerl 2no1n as Proteus

3' mor%anii / onl0 specie

!"s and 1ound infections


21/27

Providencia

Pro$idencia ( = species / P' alcalifaciens#

P' Rusti%ianii# P' rett%eri# P' stuartii)

P' rett%eri P' stuartii rease + -

Citrate + +


22/27

Yersinia

;; species6' pestis (pla%ue) / bipolar stainin% (1a0son)5%ent of bubonic pla%ue# pneumonic and septicemic pla%ues

9 ubonic / bite of infected flea9 Pneumonic

9 Prefered %ro1t. at > de% C6' enterocolitica (acute enteritis / appendictis li2e)

9 Pi%# cats and do%s9 Cold enric.ment9 3otile at R!

6' pseudotuberculosis

9 Pat.o%en in animals (tur2e0# %eese# do$es# farm anddomestic animals)


23/27

YERSINIA

et0 pin appearance

Yersinia pestis


24/27

evision !uestion ,- (haracteristic of enterobacterceae all of the

followin' ecept a- $ram ne'ati+e rod

b- /acultati+e anaerobic

c- /erment 'lucose

d- Oidase positi+e

0-Swormin' is special characteristic of

a. )seudomonades

b- )roteus

c- E. coli

d- Klebsiella 1- &ucoid colony is special characteristic of

a. )seudomonades

b- )roteus

c- E. coli

d- Klebsiella


25/27

2- Bacteria which 'i+e 'reen metallic sheen on E&B medium

is

a. )seudomonades

b- )roteus

c- E. coli d- Klebsiella

3- )olysaccharide capsule present on which of the followin'

bacteria

a. )seudomonades

b- )roteus c- Shi'ella

d- Klebsiella

4- 5hich of the followin' bacteria is urease positi+e

a. )seudomonades

b- )roteus c- E. coli

d- Klebsiella

6- 5hich of the followin' bacteria is lactose fermenter

a. )seudomonades

b- )roteus c- E. coli


26/27

5hen we inoculate members of

enterobacterceae on &ac(on7ey a'ar8

mention which or'anism 'row producin'

lactose fermentor colony and which 'row

producin' lactose fermentor colony


27/27

klebsiellaand e.!oli

2 members of the family enterobacteriaceae

Documents