18555389 skin graft powerpoint
TRANSCRIPT
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Skin GraftSkin Graft
ByByDr. Diyar A. SalihDr. Diyar A. Salih
Plastic Surgery ResidentPlastic Surgery ResidentKurdistan, SlemaniKurdistan, Slemani
Nov 26Nov 26thth, 2007, 2007
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Skin functionsSkin functions Protective barrier, against:2. Trauma3. Radiation4. Temperature changes5. Infection
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Cont. Thermoregulation, through:2. Vasoconstriction & Vasodilatation3. Insensible fluid loss control
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Skin anatomySkin anatomy
Skin varies in thickness depending on: Anatomic location / thickest in the
palm & sole of the feet, thinnest in the eyelids & postauricular region.
Sex / male thicker than female. Age / children have thin skin
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Skin layersSkin layers1. Epidermis Stratified
squamous epithelium / Keratinocytes.
No blood vessels /Nutrients from dermis by diffusion through basement membrane.
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Cont. Dermis: Papillary dermis
Thinner Loose connective tissue, containing:
1. Capillaries
2. Elastic fibers
3. Reticular fibers
4. Some collagen
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Cont. Reticular dermis:
Thicker layer Dense connective tissue, containing:
1. Larger blood vessels2. Closely interlaced elastic fibers3. Coarse, branching collagen fibers arranged in
layers parallel to the surface.4. Fibroblasts5. Mast cells6. Nerve endings7. Lymphatics8. Some epidermal appendages
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Epithelial cell sourceEpithelial cell source Epithelial cells re-epithelialize when
the overlying epithelium is removed or destroyed by;
2. Partial thickness burn3. Abrasions4. STSG harvesting.
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Cont. Source, intradermal structures
(epithelial appendages):1. Sebaceous glands2. Sweat glands3. Apocrine glands4. Hair follicles
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What’s skin graft?What’s skin graft?
Is transplantation of the skin from one part to another part (removed from its blood supply).
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TypesTypes
According to the origin: Autograft / from the same
individual Allograft / from different
individual (of the same species)
Xenograft / from different species (gene pig)
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Types, cont.Types, cont. According to the dermal thickness:
STSG (epidermis + variable thicknessvariable thickness dermis)
Thin (0.005 – 0.012 inches) Intermediate (0.012 – 0.018) Thick (0.018 – 0.030)
Could be; Meshed Sheet
FTSG (epidermis + entireentire dermis)Contains adnexal structures (sweat glands,
sebaceous glands, hair follicles & capillaries).
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THICK GRAFTS ???!!!THICK GRAFTS ???!!!
ADVANTAGES:The thicker the dermal component, the
more the characteristics of normal skin are maintained following grafting, because:
Greater collagen content Larger no. of dermal vascular
plexuses Larger no. of epithelial appendages
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THICK GRAFTSTHICK GRAFTS
DISADVANTAGES : More favorable conditions for survival
/ greater amount of tissue requiring revascularization.
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CHOICE BETWEEN FULL- AND CHOICE BETWEEN FULL- AND SPLIT-THICKNESS SG.SPLIT-THICKNESS SG.
Depends on the wound’s :2. Condition3. Location4. Size5. Aesthetic concerns
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FULL THICKNESS SKIN GRAFTSFULL THICKNESS SKIN GRAFTS
Advantages/
Ideal for the face / where local flap is inaccessible or not indicated.
Retain more characteristics of normal skin, including;
Color Texture Thickness
Less secondary contraction In children grow with the individual Greater sensory return (greater availability
of neurilemaal sheet)
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FTSG, Cont.FTSG, Cont.Disadvantages/
More primary contractures More hair follicles transferred More precarious survival (well vascularized bed) Limited range of applications, for;
Small wounds Uncontaminated wounds Well – vascularized wounds
PRIMARY CONTRACTURE: immediate recoil of a freshly harvested graft due to the ELASTIN in the dermis (the more dermis the graft has, the more primary contracture).
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FTSG DONOR SITESFTSG DONOR SITES
Closed : Primarily STSG / from another site.
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FTSG ProcedureFTSG Procedure
1. Planning ( measuring, pattern made, donor site infiltration “LA +/- Epinephrine”)
2. Harvesting / scalpel3. Donor site closed primarily.4. Graft placed.
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STSGSTSG ADVANTAGES:• Less ideal conditions for survival, broader range of
application.• Less hair follicles transferred Used to resurface :
Large wounds Line cavities Mucosal defects Flap donor sites Muscle flap
Donor site heals by epidermal appendages cells immigration & proliferation.
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Cont. Disadvantages;
More fragileCan not withstand subsequent radiation therapyMore secondary contractureDo not grow with the individualSmoother & shiner than normal skinAbnormal pigmentation tendency (pale/ white/ hyperpigmented)Donor site more painful than the recipient site
SECONDARY CONTRACTURE: contraction of a healed scar due to MYOFIBROBLAST activity (the thinner the STSG, the greater the secondary contracture).
STSG is more functional than cosmetic
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Skin graft survival (Skin graft survival (TAKETAKE))
Depends on the graft’s ability to;• Receive nutrients & vascular
ingrowth from the bed (in 3 phases, 4 theories)
• Close contact & immobilization (skin graft adherence, in 2 phases)
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Skin graft revascularizationSkin graft revascularization
Phases;• Serum imbibition;
• Lasts 24 – 48 hr• Fibrin layer forms (adhere the graft to
the bed.• Nutrient absorption into the graft (from
the bed by capillary action).
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Skin graft revascularizationSkin graft revascularization
1. Inosculation;• Recipient & donor end capillaries
aligned.
• Kissing capillaries;• Graft revascularized through kissing
capillaries.
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Graft revascularization theoriesGraft revascularization theories
• Neovascularization (invade graft)• Communication (between graft & bed
vessels)• Neovascularization + communication• Graft vasculature made up primarily
from its Original vessels before transfer.
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How to optimize TAKE?How to optimize TAKE?• Well vascular bed, seldom take in
exposed;• Bone without periosteum (despite orbit or
temporal bone)• Cartilage without perichondrium• Tendon without paratenon
• Close contact (between graft & bed);• Hematomas• SeromasThese 2 immobilize & compromise graft take.
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Skin graft adherence phasesSkin graft adherence phases
• First phaseFirst phase: Begins with placement of the graft
on the bed. Graft adhered by fibrin deposition. Lasts 72 hr.• Second phaseSecond phase: Growth of fibrous tissue & vessels
into the graft.
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Sheet graftSheet graft Definition/ Is a continuous,
uninterrupted graft. Advantages/
Superior aesthetic result Disadvantages/
Not allowing blood or serum to drain.
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Meshed graftMeshed graft Definition/ Is a sheet graft after
multiple mechanical incisions. Advantages/
• Allowing immediate graft expansion.• Cover larger area per cm2
• Allows blood & serum drainage. Disadvantages/
1. Pebbled appearance (aesthetically not acceptable).
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What will happen if a wound What will happen if a wound heals without skin graft?heals without skin graft?
Granulating wounds heal secondarily demonstrate the greatest degree of contraction & are most prone to hypertrophic scarring.
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EPITHELIAL APPENDAGES IN EPITHELIAL APPENDAGES IN THE SKIN GRAFTTHE SKIN GRAFT
Their no. depends on the dermal thickness.
Graft sweats / depend on:1. Sweat glands no. transferred2. Sympathetic reinnervation of these glands from
the recipient site.
Skin graft reinnervated from: Nerve fiber ingrowth from the recipient site. From the periphry.
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Donor site Epidermis/Regenerate from epidermal appendages cells immigration, left
in the dermis. Dermis/Never regenerates. STSG/Original donor site can be used for subsequent harvest
(dependant on donor dermis thickness). Healing/
1. By re-epithelialization from epidermal appendages within nearly 7 days according to its thickness.
2. Enhanced by moist dressing & protection from; Mechanical trauma Desiccation
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Donor site selectionDonor site selection
ConsiderConsider/2. Color3. Texture4. Thickness5. Vascularity6. Donor site morbidity
SitesSites/• Any where• Face:
Supracalvicular area Upper eyelid (small amount, very thin)
– Common sites (for STSG): Thigh Buttocks Abdominal wall
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SG postoperative careSG postoperative care Graft failure, causes;2. Hematoma3. SerromaRaising the graft, prevent revascularization.• Infection ( > 105 organism per gram of tissue)Minimized by careful bed preparation & early graft inspection
after applying to a contaminated bed.Infection at the graft donor site can converts partial thickness
dermal loss into complete thickness dermal loss.8. MobilizationInterrupt revascularization, prevented by tie-over bolster
dressing on the face & trunk, splinting on the extremities.
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Biologic dressingBiologic dressing Definition/Temporary wound coverage, eg. Large burns, necrotizing
facsiitis. Advantage/Protect the recipient bed from desiccation & further trauma until
definitive closure. Biologic skin substitutes/
1. Human allograft (take, rejected after 10 days, unless the recipient immunosuppressed as in large burns, rejection take longer).
2. Amnion3. Xenograft (pig skin), rejected before becoming vascularized
(take). Synthetic skin substitutes/
1. Silicone2. Polymers3. Composed membranes
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Human epidermis (in vitro)Human epidermis (in vitro) Human epidermis cultured in vitro to
yield sheet of cultured epithelium that will provide coverage , albeit fragile (due to lack of epidermis), for Large wounds.
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THE ENDTHE END
THANK YOUTHANK YOU