17th international congress on animal reproduction · 11:00 – 12:15 poster session 1 east exhibit...

Program

17th International Congress on Animal Reproduction

“Celebrating the Past and Embracing the Future”

Vancouver Convention CentreVancouver, British Columbia Canada

July 29-August 2, 2012

Vancouver photography courtesy of: The Vancouver Convention Centre and Tourism Vancouver.

www.BionicheAnimalHealth.com

Bioniche Animal Health discovers, develops,

manufactures and markets animal health products

worldwide. Headquartered in Canada, the Company

was formed in 1979 to develop vaccines and other

pharmaceutical-based products for the preventative

and non-antibiotic health management of animal

diseases. A key area of focus for Bioniche Animal

Health is enhancing reproduction performance.

Bioniche Animal Health has numerous achievements

in the area of assisted reproduction including;

i Manufacturing the world’s leading FSH product,

Folltropin®-V, for the superovulation of cattle.

i Being the first company to offer a USDA-approved

product, SETTLE™, as a non-antibiotic aid in the

treatment of equine endometritis.

i Representing the only FDA-approved deslorelin

product, SucroMate™ Equine, for use as an

ovulatory agent in mares.

i Developing SYNGRO®, the first full line of

non-animal origin, embryo collection and

transfer media.

i Supporting research in the field of superovulation

and FTAI in cattle

i Distributing a full complement of reproductive

hormones, including Cue-Mate®, a progesterone

insert, eCG and progesterone sponges for small

ruminants.

i Bringing Cue-Mare®, the first registered

intravaginal progesterone releasing device for

mares, to the market.

Bioniche Animal Health is committed to researching

and developing new products and protocols to meet

the changing needs of the assisted reproduction

industry. Please visit our booth (#11-12) to learn more

about our areas of research and our products.

BionicHe AnimAl HeAltH: A GloBAl leAder in Assisted reproduction

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Program at a Glance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Program Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Prizes & Awards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Networking Opportunities . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Keynote and Plenary Sessions . . . . . . . . . . . . . . . . . . . . . . . 14

Symposia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Workshops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Poster Presentations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Ancillary Meetings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Industry-Sponsored Symposia . . . . . . . . . . . . . . . . . . . . . . . 60

Sponsors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Exhibitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Table of Contents

2

Program At a Glance

Time Sun. July 29 Mon. July 30 Tues. July 31 Wed Aug. 1 Thurs. Aug. 2

7:30 7:30 - 8:00Early Morning Coffee

7:30 - 8:00Early Morning Coffee

7:30 - 8:00Early Morning Coffee

8:15-10:30Plenary 1

Opening Ceremony

Keynote Speaker

Simmet Prize & Address

8:15-10:30Plenary 2

Post-natal Consequences of Prenatal Environment

8:15-10:30Plenary 3

Beyond the Bench:Expanding the Impact of

Reproductive Science

8:15-10:30Plenary 4

Global Health and the Future of Domestic

Animal Reproduction

8:30

9:00

9:30

10:0010:00 – 17:00

UBC Dairy Centre Tour

(not included in Congress

Registration)

10:30 10:30-11:00Coffee Break

10:30-11:00Coffee Break



11:00

11:00-12:15Poster Session 1



11:00-12:00Plenary 5

Keynote SpeakerClosing Ceremony

11:30

12:00

12:15 – 13:45Industry-Sponsored Lunch Symposium

Sponsored by Pfizer Animal Health

12:15 – 13:45Industry-Sponsored Lunch Symposium

Sponsored by Bioniche

12:15 – 13:45Exhibits

12:30

13:00

13:3013:45 – 16:45Symposia 1-4

1. Stem & Germ Cell 2. Cloning Research3. Oocyte & Embryo4. Sperm Development

13:45 – 16:45Symposia 5-8

5. Cryopreservation6. Contraception 7. Genetic Diversity8. Ovarian Function

13:45 – 16:45Symposia 9-12

9. Production & Fertility10. Reproductive Toxicology11. Male Reproduction12. Immunology of Pregnancy

14:00

14:30

15:00 15:00 – 15:30Coffee Break

15:00 – 15:30Coffee Break

15:00 – 15:30Coffee Break

15:30Symposia 1-4 (continued)

Symposia 5-8(continued)

Symposia 9-12(continued)

16:00

16:30

16:45 – 18:15Industry-Sponsored

Symposium

Sponsored by Bioniche

17:00

17:00 – 18:30

Workshops 1-9

17:00 – 18:30

Workshops 10-18

17:30

18:00 Welcome Reception

Sponsored by Merial

19:30

Salmon Bake Student Social ICAR Dine & Dance20:00

22:30

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Program Schedule

Sunday July 29, 2012

10:00 – 17:00 UBC Dairy Centre Tour

15:00 – 19:30 Registration

18:00 – 20:00 Welcome Reception sponsored by Merial East Exhibit Hall B

Monday July 30, 2012


07:30 – 08:15 Early Morning Coffee East Exhibit Hall B

08:15 – 10:30 Plenary Session 1: Opening Session & Simmet Prize East Ballroom ABC

08:15 – 08:40 Opening Ceremony

08:40 – 09:30 An amazing 10 years: The discovery of egg and sperm in the 17th century Matthew Cobb

09:30 – 10:30 Simmet Prize & Address: New horizons on an old landscape - oxidative stress, DNA damage and apoptosis in the male germ line John Aitken

10:30 – 11:00 Coffee Break East Exhibit Hall B

11:00 – 12:15 Poster Session 1 East Exhibit Hall B

12:15 – 13:45 Industry-Sponsored Lunch Symposium – sponsored by Pfizer Animal Health East Meeting Room 1-3

13:45 – 16:45 Symposia Sessions 1 - 4

Symposium 1:

Stem and germ cell research

and its application

East Ballroom C

Symposium 2:

Cloning research and its application

East Meeting Room 8

Symposium 3:

Oocyte and embryo health

East Ballroom B

Symposium 4:

A new understanding of sperm development

and function

East Ballroom A


15:30 – 16:45 Symposia Sessions 1 – 4 continued

17:00 – 18:30 Workshops 1 - 9

19:30 – 22:30 Salmon Bake (ticket purchase required) Pan Pacific Hotel Vancouver – Oceanview Suites 1-4

Workshop 1

Dairy cattle reproduction

East Ballroom B

Workshop 2

Equine reproduction

East Meeting Room 8

Workshop 3

In vitro embryo production

East Ballroom A

Workshop 4

Buffalo (Bubulus)

reproduction

East Meeting Room 10

Workshop 5

Genomic & proteomic tools in reproduction

research

East Ballroom C

Workshop 6

Emerging roles of seminal

plasma


Workshop 7

Biostatistics

East Meeting Room 7

Workshop 8

Wild ungulate reproduction


Workshop 9

Tools to study reproductive

behavior


4

Tuesday July 31, 2012



08:15 – 10:30 Plenary Session 2: Post natal consequences of prenatal environment East Ballroom ABC

08:15 – 09:00 Effects of exposure to environmental chemicals during pregnancy on the development of the male and female reproductive axes Paul Fowler

09:00 – 09:45 Effects of maternal diet on sexually dimorphic responses in conceptuses and offspring Cheryl Rosenfeld

09:45 – 10:30 Maternal dietary restriction on ovarian folliculogenesis and fertility in bovine offspring Alex Evans



12:15 – 13:45 Industry-Sponsored Lunch Symposium – sponsored by Bioniche East Meeting Room 1-3


Symposium 5:

Cryopreservation of gametes, embryos, organs and tissues

East Ballroom B

Symposium 6:

Contraception and sup-pression of reproductive

function

East Meeting Room 8

Symposium 7:

Genetic selection and preservation of genetic

diversity

East Ballroom C

Symposium 8:

Advances in the understanding of ovarian function

East Ballroom A

15:00 – 15:30 Coffee BreakEast Exhibit Hall B


16:45 – 18:15 Industry-Sponsored Symposium - sponsored by Bioniche East Meeting Room 1-3

19:30 – 22:30 Student Social (ticket purchase required) Shark Club

Wednesday August 1, 2012



08:15 – 10:30 Plenary Session 3: Beyond the Bench: expanding the impact of reproductive science East Ballroom ABC

08:15 – 09:00 Exploiting multimedia in reproductive science education-research findings Phil Senger

09:00 – 09:45 Ethics, politics and protests: Using contentious issues in reproductive science as educational opportunities James Knight

Program Schedule (Continued)

5

Wednesday August 1, 2012 (Continued)

09:45 – 10:30 Reproductive science in the Global Village Peter Chenoweth




Symposium 9:

The inter-relationship of productivity and

fertility

East Ballroom B

Symposium 10:

Reproductive toxicology and the environment

East Meeting Room 8

Symposium 11:

Applied research in male reproduction

East Ballroom A

Symposium 12:

Immunology of pregnancy

East Ballroom C

15:00 – 15:30 Coffee BreakEast Exhibit Hall B


17:00 – 18:30 Workshops 10 - 18

Workshop 10

Beef cattle reproduction

East Ballroom C

Workshop 11

Small ruminant

reproduction

East Meeting Room 8

Workshop 12

Canine & feline reproduction


Workshop 13

Camelid reproduction

East Meeting Room 7

Workshop 14

Sperm & embryo sexing

East Ballroom B

Workshop 15

Imaging in reproduction


Workshop 16

Reproductive pathology


Workshop 17

Swine reproduction


Workshop 18

Diagnostic tools for

gametes and embryos

East Ballroom A

19:30 – mid-night

ICAR Dine and Dance (ticket purchase required) Pan Pacific Vancouver – Crystal Pavilion

Thursday August 2, 2012


08:15 – 10:30 Plenary Session 4: Global health and the future of domestic animal reproduction East Ballroom ABC

08:15 – 09:00 Using domestic animals for biomedical research Eckhard Wolf

09:00 – 09:45 Animal models and regenerative medicine Steven Stice

09:45 – 10:30 Global food systems: technological advancements to feed the world Gregg BeVier

10:30 – 11:00 Coffee Break East Ballroom Foyer

11:00 – 12:00 Plenary Session 5: Keynote & Closing East Ballroom ABC

11:00 – 11:45 A Moving Landscape of Wildlife Genetics Stephen J. O’Brien

11:45 – 12:00 Closing Ceremony

Program Schedule (Continued)

6

Welcome

Dear Colleagues:

Welcome to the 17th International Congress on Animal Reproduction (ICAR 2012). An

icon of animal reproductive biology, the ICAR is like the Olympics - it has emerged every

4 years since 1948 in some of the most remarkable cities in the world. This is the first

time Canada has ever hosted ICAR and we are excited to have you in Vancouver.

For the Local Organizing Committee, ICAR 2012 began 5 years ago. The LOC has spent

countless hours developing the scientific and social programs, identifying and inviting

world-class speakers, reviewing manuscripts, coordinating poster sessions, satellite

symposia, contracting services, venues and hotels, and promoting what we hope will be

an outstanding Congress. Give them a pat on the back when you get a chance!

The 4-day Congress includes Plenary Sessions, concurrent Symposia and Workshops, and Poster sessions (both traditional

and electronic posters). Invited speakers will present on topics that span the breadth of reproduction from the sperm

to the egg, from genomic conservation to cryopreservation, and from global food systems to reproductive education

systems. The peer-reviewed proceedings include 60 full-length review articles and more than 550 abstracts. Opening and

closing keynote addresses by distinguished authors/scientists encapsulate the theme of ICAR 2012 - Celebrating the past

and embracing the future.

We gratefully acknowledge the financial support of several organizations and institutions, as well as the livestock and bio-

pharmaceutical industries. Furthermore, we congratulate Dr. John Aitken, recipient of the prestigious ICAR Simmet Prize for

Assisted Reproduction.

Welcome to Vancouver! Enjoy the meeting, make new friends and meet old friends, and enjoy the hospitality and breath-

taking scenery of Canada.

Gregg P. Adams DVM, PhD, Dipl ACT

Chair, ICAR 2012 Local Organizing Committee

7

General Information

Registration DeskThe registration desk is located on the main lobby level of the Vancouver Convention Centre - East Building, and will be open at the following times:

Sunday, July 29 15:00 – 19:30Monday, July 30 07:00 – 17:30Tuesday, July 31 07:15 – 17:00Wednesday, August 1 07:15 – 17:30Thursday, August 2 08:00 – 11:00

Name Badge PolicyNames badges will be provided to all delegates and participants when they check-in at the ICAR 2012 Registration Desk. It is your admission pass to the scientific sessions, the exhibit hall and social events. Delegates are required to wear their name badge at all times and will not be granted access to the Congress without it. There is a $60.00 CAD reprint fee for any lost or misplaced badge. Please wear your name badge at all times. This is to ensure that access to the Congress is properly managed.

Dress CodeCasual business attire is recommended for the Congress.

Speaker Ready RoomThe Speaker Ready Room is located in East Meeting Room 6, on the East Meeting Level of the Vancouver Convention Centre. An audio-visual technician will be available to assist with your presentation. Due to the large number of

presentations scheduled throughout the Congress, speakers are asked to visit the Speaker Ready Room the day before their presentation. The Speaker Ready Room will be open to assist you at the following times:

Sunday, July 29 15:00 – 19:00Monday, July 30 07:00 – 17:00Tuesday, July 31 07:00 – 17:00Wednesday, August 1 07:00 – 17:00

Poster PresentationsElectronic and paper poster presentations are scheduled from 11:00 – 12:15 on Monday July 30, Tuesday July 31 and Wednesday August 1 inside East Exhibit Hall B on the Convention Level of the Vancouver Convention Centre. Please refer to the e-poster and paper poster presentation schedule on page 31.

My ProgramCustomize your own Congress schedule, and see full details of each session and abstract online. Visit: www.icar2012.com/myprogram

ICAR 2012 Congress Secretariatc/o MCI Vancouver200 -1444 Alberni StVancouver, BC V6G 2Z4Telephone: 604-688-9655 ex. 2E-Mail: [email protected]

ICAR Affairs

The International Congress on Animal Reproduction (ICAR) is a non-profit international organization that conducts conferences on Animal Reproduction. Founded in 1948, ICAR has held a major conference every 4 years in countries throughout the world. The theme of the congress is animal reproduction, including animal physiology, animal pathology and reproductive technology. Each conference has invited plenary and symposia papers, other contributed papers, workshops, and a trade exhibition. ICAR has no

members as such, but is governed by a Standing Committee of representatives of more than 40 countries. An Executive Committee oversees general business according to the Constitution and By-Laws adopted in 1992 and modified in 2002. The Secretary General is responsible for routine administration. The Executive Committee of ICAR is elected from and by members of the Standing Committee, with due reference to maintaining an appropriate geographical representation.

International Congress on Animal Reproduction

http://www.icar2012.com/myprogram

mailto:[email protected]

http://www.mci-group.com

8

Committee Members

ICAR 2012 Standing Committee

Ali Akbar Fouladi-Nashta United Kingdom Matt Lucy USAVita Antane Latvia Graeme Martin Australia Jörg Aurich Austria Chris Maxwell AustraliaPietro Baruselli Brazil Scott McDougall New ZealandHenk Bertschinger South Africa Pascal Mermillod FranceGabriel Bo Argentina Jose Morales-Carnallo CubaDaniel Cavestany Uruguay Janko Mrkun SloveniaJongbo Chen China Amarjit Nanda IndiaLuzbel de la Sota Argentina Amir Niasari-Naslaji Iran Michael Diskin Ireland Stanislaw Okrasa PolandGareth Evans Australia Torsteinn Olafsson IcelandWenche Farstad Norway Humberto Osvaldo Cisale ArgentinaGyörgy Gábor Hungary Oswin Perera Sri LankaFulvio Gandolfi Italy Morten Petersen DenmarkAhmet Gümen Turkey Bambang Purwantara IndonesiaHans Gustafsson Sweden József Rátky HungaryPeter Hansen USA Jiri Rubes Czech RepublicSG Hassan UAR Egypt Hosup Shim KoreaMarc Henry Brazil Harald Sieme GermanyUdo Herbert Nigeria Lulu Skidmore UAE EmiratesBernd Hoffmann Germany Mongkol Techakumphu ThailandAntónio Horta Portugal William Vale BrazilHiroshi Imai Japan Ann Van Soom Belgium Fredi Janett Switzerland Juan Vazquez Spain Jennifer Juengel New Zealand Peter Vos NetherlandsTerttu Katila Finlad Andres Waldmann EstoniaAndres Kowalski Venezuela Milo Wiltbank USAEvija Liepina Latvia David Wolfenson IsraelKehuan Lu China Liguo Yang ChinaYangqing Lu China

ICAR Executive Committee

President: Henk Bertschinger (South Africa) Vice President: Oswin Perera (Sri Lanka) Secretary General: Gareth Evans (Australia)

Executive Committee (Ordinary Members)

Gregg Adams (Canada) György Gábor (Hungary) Fulvio Gandolfi (Italy) Peter Hansen (USA) Marc Henry (Brazil)

ICAR 2012 Local Organizing Committee

Gregg Adams (Chair)Lori AdamsMuhammad AnzarOrleigh BogleAli HonaramoozReuben Mapletoft Roger PiersonRajadurai RajamahendranJaswant SinghPaula TribuloJimena Yapura

ICAR 2012 LOC Subcommittee Chairs

Scientific Program: John Kastelic & Jacob ThundathilPublications: Jaswant SinghAwards: Divakar Ambrose & Reuben MapletoftIndustry Advisory: Reuben MapletoftCommunications: Muhammad AnzarStudent Advisory: Orleigh Bogle & Jimena YapuraSocial/Cultural: Lori Adams

9

Continuing Education Credits

ICAR 2012 will provide each delegate with a Certificate of Attendance, which will be found in your Congress Bag. It will indicate the number of hours for full attendance at the

Congress, and it will be the responsibility of the individual delegate to claim attendance with his/her respective licensing body or society.

Prizes and Awards

SIMMET PRIZE FOR ASSISTED REPRODUCTION

The ICAR Simmet Prize recognizes an active scientist for outstanding basic and applied research in the area of Assisted Reproduction in Animals. The Prize is awarded every four years at the ICAR meeting and comes with a prize of 50,000 Euro. The Simmet Prize is sponsored by the Simmet Family in honor of Dr. Ludwig Simmet, founder of Minitube.

Dr. R. John Aitken is the 2012 recipient of the Simmet Prize.

Dr. Aitken’s numerous landmark studies in male infertility in domestic livestock and humans have influenced laboratories and allied industries around the world.

His research career began with a PhD in reproductive biology from the University of Cambridge under

the supervision of RV Short. Following post-doctoral positions at the Universities of Edinburgh and Bordeaux, he joined the World Health Organization in Geneva. In 1977, he was appointed to the prestigious MRC Reproductive Biology Unit at the University of Edinburgh to establish a research group in developmental biology with clinical outreach into male infertility. In 1992, he was awarded an Honorary Professorship within the Faculty of Medicine and in 1998 received a ScD from the University of Cambridge in recognition of his research contributions to gamete biology. He then moved to the University of Newcastle, NSW, Australia, as Chair of Biological Sciences, and later, Director of the ARC Centre of Excellence in Biotechnology and Development.

Dr. Aitken has published more than 450 research articles, given more than 300 invited lectures and filed 12 patents. His work has been cited more than 13,000 times (h-index of 60). He has been elected a Fellow of both the Royal Society of Edinburgh and the Australian Academy of Science. Dr. Aitken has supervised 11 MSc students and 23 PhD students.

Dr. Aitken pioneered the development of objective methods for measuring sperm movement, beginning with time-lapse photography and culminating in CASA systems that are now commonplace in modern-day theriogenology. He also developed a novel technique for monitoring the acrosome reaction in live cells, and was instrumental in developing heterologous in vitro fertilization assays of sperm function that are used to monitor male fertility in large domestic animals. He subsequently developed biochemical parameters to predict male infertility. In the course of these studies he pioneered the fundamental concept that oxidative stress is a major determinant of sperm function and DNA damage and developed fluorometry/ flow cytometry based assays that are now widely used in diagnosing male infertility in a range of mammalian species.

Dr. Aitken is among the first reproductive biologists with the vision to implement advanced mass spectrometry platforms dedicated to proteomics and metabolomics in gametes and embryos. Dr. Aitken is an exceptional scientist who has developed a legacy of well-trained graduate students and post doctoral fellows. Dr. Aitken truly personifies the intent of the Simmet Prize in Animal Reproduction.

10

Prizes and Awards (Continued) SISTER SOCIETY AWARDS TO YOUNG SCIENTISTS

Four Young Scientists were selected by ICAR Sister Societies. Each of the award recipients will be delivering a presentation during the Congress.

Ramiro Alberio Division of Animal Sciences (School of Biosciences), University of Nottingham, UK Nominated by the Society for Reproduction and Fertility (SRF) Presentation: Recent advances in stem and germ cell research in pigs (Symposium 1)

Ramiro Alberio is a Lecturer in Developmental Epigenetics at the Division of Animal Sciences (School of Biosciences), University of Nottingham, UK. He received his DVM from La Plata University (Argentina) and his PhD from Munich University (Germany). His research interests are epigenetic reprogramming of somatic cells, germ cell biology and pluripotency.

Tamara Leahy University of Queensland - Australia Nominated by the Society for Reproductive Biology (SRB) Presentation: Exploring seminal plasma and its effects on ram sperm characteristics through processing (Symposium 5)

Tamara received a Bachelor of Animal and Veterinary Bioscience at the University of Sydney, Australia in 2006. She completed her PhD at the same institution, investigating the effect of seminal plasma proteins on ram sperm function during processing for sex‐sorting or storage. In 2010 she joined the University of Queensland, Australia as a Post‐Doctoral Research Fellow analysing bull sperm membrane proteins. Tamara has published 8 original research articles and 3 review articles.

Rebecca L. Robker Department of Obstetrics and Gynaecology Adelaide University - Australia Nominated by the Society for the Study of Reproduction (SSR) Presentation: The critical roles of progesterone receptor (pgr) in ovulation, oocyte developmental competence and oviductal transport (Symposium 8)

Dr. Rebecca Robker is a Research Fellow at The Robinson Institute and School of Paediatrics and Reproductive Health at the University of Adelaide, Australia. Her research is focused on identifying cellular pathways in the ovary that regulate oocyte quality and ovulation, particularly those incorporating maternal hormone, immunological and metabolic signals.

Ulrike Taylor Institute of Farm Animal Genetics, Department of Biotechnology - Germany Nominated by the European Society for Domestic Animal Reproduction (ESDAR)

Presentation: Impact of nanoparticles on germ cell viability and functionality (Symposium 11)

Ulrike Taylor studied veterinary medicine in Hanover, Germany and Edinburgh, Scotland. Since 2005, she has worked at the Institute of Farm Animal Genetics, where she finished her doctorate thesis in 2007 and now holds a postdoctoral position. Her research focusses on reproductive immunology and repro-toxicology of nanoparticles.

http://www.icar2012.com/53

http://www.icar2012.com/53

http://www.srf-reproduction.org/


http://www.srb.org.au/



http://www.ssr.org/

http://www.ssr.org/

http://www.esdar.org/

http://www.esdar.org/

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Prizes and Awards (Continued) STUDENT TRAVEL AWARDS

Over 60 applications were received for the Student Travel Awards. We thank each and every one of our applicants. The Awards Committee had a very difficult task of selecting the award recipients out of such a large pool of worthy applicants. Congratulations to the award recipients:

Mazdak Salavati (Iran) Orsolya Gabriella Balogh (Hungary) Elisa Marelli (Italy) Emy Elizabeth Varughese (India) Hyun Ju Oh (Korea) Eduardo Arashiro (Brazil) Andres Tribulo (Argentina) Annabel Bergmann (Germany)Jessica Rickard (Australia) - award sponsored by the Society for Reproductive Biology (SRB)

TRAVEL AWARD FOR YOUNG SCIENTISTS FROM DEVELOPING COUNTRIES

Over 35 applications from 12 countries were received for the Young Scientist Awards. We thank each and every one of our applicants. Congratulations to the award recipients:

Maria Isabel Vazquez (Argentina) Ana Izabel Villaverde (Brazil) Mohamed El-Sayed El-Sharawy (Egypt) Amjad Riaz (Pakistan) Monika Kaczmarek (Poland) Nutthee Am-in (Thailand) Alireza Moghadam Masouleh (Iran) Carolina Griselda Luchetti (Argentina) Ajeet Kumar (India) Joanna Kochan (Poland)

C: 0M: 100Y: 80K: 55

C: 0M: 100Y: 70K: 40

C: 0M: 100Y: 79K: 20

www.semex.com

into tomorrow together

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Networking Opportunities

Welcome ReceptionSunday, July 29 18:00–20:00 Dress Suggestion: Casual/Business-casual Location: Vancouver Convention Centre, East Exhibit Hall B

The ICAR 2012 Organizing Committee invites all delegates to the Welcome Reception. Start off the Congress with this opportunity to meet colleagues, network, meet exhibitors and catch up with friends! Admission to the Welcome Reception is included for registered delegates and registered accompanying persons.

Salmon BakeMonday, July 30 19:30 – 22:30 Dress Suggestion: Casual Cost: $85.00 Location: Pan Pacific Vancouver - Oceanview Suites 1-4

This must-attend evening at the Pan Pacific Hotel Vancouver will feature a menu to highlight the taste of British Columbia. It is a perfect opportunity to enjoy some local Vancouver musical, artistic and magical talents, and the stunning view of the North Shore Mountains. Other food options will be available, for those that prefer something other than salmon.

Extra tickets available at Registration on a limited basis!

Hockey Shoot-OutMonday, July 30 - Wednesday, August 115:00 - 16:00Location: East Exhibit Hall B

A truly Canadian Challenge! Join us in the Exhibit Hall every afternoon for the Hockey Shoot-Out, for your chance to win a great prize!

Student NightTuesday, July 31 20:00 to closing Dress Suggestion: Casual Cost: $20.00 Location: Shark Club Vancouver, 180 West Georgia Street

An event organized especially for students, it will be an excellent opportunity to meet colleagues, make new friends and dance the night away. Admission to the Student Night includes appetizers and one drink (draft beer). All delegates and registered accompanying persons are also welcome. Tickets must have been purchased at Registration (no tickets for sale on-site).

Guest speaker: Dr. John ChallisJohn Challis PhD DSc is University Professor Emeritus in Physiology, Ob-Gyn and Medicine at the University of Toronto, and Adjunct Professor at SFU, UBC and the University of Western Australia. He is Principal of LHC: Life Health Consulting. He has published more than 500 articles and papers, 400 of these in the peer review literature, has an H-index of 60, trained more than 90 graduate students and postdoctoral fellows and held more than $25M in peer review funding. He is currently working with Universities and affiliated institutions in Vancouver to develop a joint graduate program in translational public health sciences.

ICAR Dine & DanceWednesday, August 1 19:30 – Midnight Dress Suggestion: Smart Business/Cocktail Cost: $125.00Location: Pan Pacific Vancouver – Crystal Pavilion

Please join us in the beautiful Crystal Pavilion for an evening of unforgettable live music and award-winning cuisine. Featuring the local band “Ten Souljers”, we can’t think of a better way to wrap up a successful Congress than with friends and colleagues new and old!

Extra tickets available at Registration on a limited basis!

THE MICHELSON GRANTSThe Foundation seeks proposals for up to $250,000 per year for up to 3 years of funding for research in pursuit of permanent contraceptives for male and female cats and dogs.The first step in the Michelson Grant process is submission of a Letter of Intent (LOI).For further details, visit: http://michelson.foundanimals.org.

THE MICHELSON PRIZEA $25 million prize will be awarded to the first entity to provide to Found Animals a safe, effective, and practical non-surgical sterilant for use in cats and dogs. The winning entry for the Michelson Prize in Reproductive Biology must meet the following criteria:

• Single dose, permanent non-surgical sterilant• Safe and effective in male and female cats and dogs• Suitable for administration in a field setting• Viable pathway to regulatory approval• Reasonable manufacturing process and cost

The Michelson Prize & Grants in Reproductive Biology

®

Apply for Research Grants

The Michelson Prize & Grants is open to any entity from any nation. Found Animals encourages scientists from a diverse range of fields to compete for the Michelson Prize & Grants.

For more information about the Michelson Prize & Grants, visit:http://michelson.foundanimals.org

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Keynote and Plenary Sessions

Plenary Session 1: Opening Session & Simmet Prize Monday, July 30 201208:15 – 10:30East Ballroom ABCChair: William C. Wagner

08:50 – 09:40Matthew Cobb, University of Manchester United KingdomAn amazing 10 years: The discovery of egg and sperm in the 17th century.

Matthew Cobb is a professor of zoology and Associate Dean for Social Responsibility, University of Manchester, UK. Author of the THE

EGG AND SPERM RACE (Simon & Schuster / Bloomsbury, 2006) and THE RESISTANCE (Simon & Schuster, 2009), he is a frequent contributor to the Times Literary Supplement and Journal of Experimental Biology.

Abstract: “Before reproduction: How we discovered the secrets of sex.” The scientific identification of the key components of sexual reproduction – eggs and sperm – took place during an amazing explosion of discovery in the 1660s and 1670s. And yet despite this, their respective roles remained unclear for another 170 years. Why did this take so long? And what did people think before these discoveries? By tracing the history of this major milestone in human knowledge, we can also gain insight into our current knowledge, and the boundaries we may be unwittingly trapped by.

09:40 – 10:30 R. John Aitken, University of Newcastle, AustraliaThe 2012 recipient of the Simmet Prize for Assisted ReproductionNew horizons on an old landscape - oxidative stress, DNA damage and apoptosis in the male germ line.

R. John Aitken is Laureate Professor of Biological Sciences and Director of the Priority Research Centre in Reproductive Science at the University of Newcastle. He is a Fellow of the Royal Society of Edinburgh and the Australian Academy of Science. He has published more than 450 research articles in gamete and developmental biology, which have been cited more than 14,000 times.

Abstract: Practical applications in assisted reproduction ultimately depend upon a knowledge of how the reproductive system works at the cellular level. Recent insights in this area have helped engineer improved methods for the diagnosis and management of infertility and generate innovative methods for selection and preservation of mammalian gametes.

Plenary Session 2: Post natal consequences of prenatal environment Tuesday, July 31 201208:15 – 10:30East Ballroom ABCChair: Kevin Sinclair

08:15 – 09:00Paul Fowler, University of Aberdeen, UKEffects of exposure to environmental chemicals during pregnancy on the development of the male and female reproductive axes

Paul A. Fowler (B.Sc. Hons Zoology, Ph.D) is professor of Translational

Medical Sciences and lead for the Cell, Developmental and Cancer Biology research programme at the University of Aberdeen, UK. Since the late 1990s his research has focused on the effects of environmental chemicals on fetal development and subsequent reproduction in both human pregnancy and in animal models (see: http://www.abdn.ac.uk/ims/staff/details.php?id=p.a.fowler ).

Abstract: The developing fetus is routinely exposed to a wide range of ubiquitous chemicals, both natural and anthropogenic,



15

Keynote and Plenary Sessions (Continued) in the environment. These are typically at relatively low concentrations and in complex mixtures. Examples from both animal and human models will be used to inform on potential consequences of in-utero exposure to exogenous chemicals for reproduction.

09:00 – 09:45Cheryl Rosenfeld, University of Missouri, USAEffects of maternal diet on sexually dimorphic responses in conceptuses and offspring

Cheryl S. Rosenfeld, DVM, PhD is a graduate of the University of Illinois at Urbana-Champaign and the University

of Missouri. Her research emphasis is on how maternal diet and in utero exposure to endocrine disrupting compounds epigenetically alters conceptus development and offspring outcomes, including neurobehavioral abnormalities.

Abstract: While sexual differentiation is considered as the onset of differentiation of the male or female gonads, mounting evidence indicates that sex differences in developmental programming are established as early as the zygotic stage. Genetic and epigenetic differences between the sexes might govern how each responds to shifts in their early environment, including in the uterus or culture dish in the case of in vitro cultured pre-implantational embryos. Even if no differences are evident between the sexes at birth, divergent conceptus responses to surrounding changes, such as maternal diet and exposure to endocrine disrupting compounds might predispose one sex over the other to later adult onset diseases, otherwise termed developmental origin of health and disease (DOHaD). This review examines the evidence to date that male and female zygotes and conceptuses diverge in their responses to shifting environmental conditions and whether these contrasting sexually dimorphic responses underpin later DOHaD outcomes, namely neurobehavioral changes.

09:45 – 10:30 Alex Evans, University College, IrelandMaternal dietary restriction on ovarian folliculogenesis and fertility in bovine offspring

Abstract: Mammals such as cattle, swine, sheep and humans are born with a highly variable number of ovarian follicles

and oocytes that dwindle during ageing. This variation in the ovarian reserve is reflected in the numbers of antral follicles in the ovaries at all ages after birth. Since numbers of follicles in ovaries are determined during gestation, the role of maternal nutrition and health during gestation (at time of ovarian development in their fetuses) has been investigated as factors that may impact oogonia proliferation and thus follicle numbers postnatally. Both nutrition and health were shown to impact numbers of follicles in offspring and cows with a relatively high number of antral follicles were shown have higher pregnancy rates, shorter calving to conception intervals and fewer artificial inseminations compared with cows with a lower number of follicles; heifers with many follicles had higher pregnancy rates than those with fewer follicles. This review will highlight the importance of the maternal environment during gestation in determining the size of the ovarian reserve in offspring and the contribution of the ovarian reserve to subsequent fertility in cattle.

16

Keynote and Plenary Sessions (Continued) Plenary 3Wednesday, August 1, 201208:15 – 10:30East Ballroom ABCChair: John Kastelic

08:15 – 09:00 Phil Senger, Washington State University, USAExploiting multimedia in reproductive science education-research findings

P.L. Senger is Professor Emeritus at Washington State University and President of Current Conceptions, Inc. Dr. Senger has a BS from North Carolina

State University and a MS and PhD from Virginia Tech. He is the author of “Pathways to Pregnancy and Parturition, 2nd Rev. Ed.” Current Conceptions, Inc. is the publisher of the textbook and is currently developing multimedia content to explain complex mechanisms in reproductive science. This content is not only useful in academic venues but is intended as an educational medium to serve a wide variety of people within the general public.

Abstract: The technology exists to explain complex topics using multimedia presentations in which digital animation and 3-dimensional anatomical reconstruction greatly reduce the time required for delivery while at the same time improving learning. We have designed and produced multimedia programs to teach complex mechanisms in various topics within reproductive physiology. Demonstrations will be presented in the session. The programs have been tested in controlled experiments involving over 1,000 university students and 100 OB/GYN patients. Data from these experiments indicate that these multimedia presentations can result in “Higher Learning @ Higher Speeds”.

09:00 - 09:45James Knight Virginia Tech, USAEthics, politics and protests: Using contentious issues in reproductive sciences as educational opportunities

James W. Knight, M.S., Ph.D., is a Professor of Reproductive Physiology in the Department of Animal and Poultry Sciences at Virginia Tech, Blacksburg,

VA, U.S.A. Dr. Knight has been heavily involved in teaching and advising both undergraduate and graduate students throughout his career. Dr. Knight had been the recipient of numerous teaching awards at the university and national levels.

Abstract: Contentious issues can be utilized in the classroom to create reflective dialogue among students, leading to both a greater understanding and enhanced appreciation of alternative viewpoints. Exploring the scientific, ethical, moral, political, legal, and societal aspects of contentious reproductive issues provides ideal subject matter for developing critical thinking skills.

09:45 – 10:30Peter Chenoweth Charles Sturt University, AustraliaReproductive sciences in the Global Village

Peter Chenoweth is Adjunct Professor of Veterinary Reproduction at Charles Sturt University, Australia and current President of the Association for Applied

Animal Andrology. Books include Beef Practice: Cow-Calf Production Medicine (Blackwell 2005), and 2 volumes of Applied Animal Andrology (Nottingham University Press)

Abstract: Livestock are vitally important for developing countries, and efficient and viable animal production depends upon effective animal reproduction. Attention to areas such as health, nutrition and genetics in addition to applying principles of good reproductive management are required. Although resources are limited, progress can be made via relatively minor managerial changes at the local level of small, mixed farms.

17

Keynote and Plenary Sessions (Continued) Such changes are a necessary prerequisite for the application of more sophisticate approaches. Both the dissemination and acceptance of information on what constitutes good reproductive management face many challenges. This review examines a number of the issues involved in meeting these challenges including the relevant roles of rural extension, biotechnology, biosecurity, research and institutional capacity. It is concluded that current programs aimed at raising livestock productivity in developing countries would benefit from increased focus on animal reproduction.

Plenary 4Thursday, August 2, 201208:15 – 10:30East Ballroom ABCChair: John Dobrinksy

08:15 – 09:00 Eckhard Wolf, Gene Center Munich, GermanyUsing domestic animals for biomedical research

Eckhard Wolf is Head of the Institute of Molecular Animal Breeding and Biotechnology at the Gene Center Munich. His research interests are

mechanisms of embryo maternal communication and the development of tailored pig models for human diseases. Abstract: The development of new therapies requires appropriate animal models for efficacy and safety studies. Although rodent models are widely used for biomedical research, they are not always predictive for clinical outcomes in human patients. Efficient and precise techniques for the genetic modification of pigs facilitate the generation of tailored large animal models which mimic human disease mechanisms at the molecular level. This will be exemplified by our genetically engineered pigs for diabetes research, which may help to translate therapeutic principles developed in rodents into clinical application.

09:00 – 09:45Steven Stice, Regenerative Bioscience Center, Greece Animal models and regenerative medicine

Steve Stice is a Georgia Research Alliance Eminent Scholar endowed chair and professor. Stice was a co-founder and chief scientific officer at

Advanced Cell Technology, Inc., a stem cell company that is currently in clinical trials for a rare disease affecting sight. His current research activities range from human to animal stem cell applications and he will present his research on induced pluripotent stem cells.

09:45 – 10:30 Gregg BeVier, USA, Global food systems: Technological advancements to feed the world

Abstract: Gregg joined the Bill and Melinda Gates Foundation in June 2009. He is in Agricultural Development and on the Market Access team as a Senior

Program Officer responsible for livestock grants. Gregg has twenty-eight years of experience as a business executive and has managed an international genetics business (PIC), an integrated livestock operation (Premium Standard Farms) and a division of a global animal health business for Merial. Gregg has also helped St. Matthew’s University, School of Veterinary Medicine establish a clinical program for fourth year students. He has been involved with assisting entrepreneurs in crop biopesticides, livestock vaccines, genetically modified rice and livestock imaging technology. Gregg is a graduate of the University of Illinois with a B.S. in Agriculture Science, M.S. in Animal Science, Doctor of Veterinary Medicine and an MBA. He has received the Distinguished Alumni Award from the College of Veterinary Medicine at the University of Illinois.

18

Keynote and Plenary Sessions (Continued) Plenary 5Closing Keynote AddressThursday, August 2, 201211:00 – 12:00East Ballroom ABCChair: Gregg Adams

11:00 – 11:45Stephen J. O’Brien, National Cancer InstituteA Moving Landscape of Wildlife Genetics.

Stephen J. O’Brien has been Chief of the Laboratory of Genomic Diversity at the National Cancer Institute (NCI), National Institutes of Health (NIH) since 1986. Dr. O’Brien is recognized

for his research contributions in human and comparative genomics, genetic epidemiology, HIV/AIDS, retro-virology, Bioinformatics and biodiversity research. He is also the

author of Tears of the Cheetah- Roar of the Lion- Uncommon Glimpses to Survival in the Wild (A Moving Landscape of Wildlife Genetics).

Abstract: His lecture will connect some recent applications of conservation genetics and natural history to uncover long-forgotten adaptive adventures that left their footprints in the genomes of lions, cheetahs and humankind. Illustrative examples will describe how scientists can track the emergence and progression of deadly outbreaks in wildlife species that reveal unfathomed threats to their very existence. How these can help to reverse extinction events and also to unlock medical secrets will be the lessons learned from his lecture.

11:45 – 12:00Closing Ceremony

Bovine/buffalo Embryo technologies

• In vitro produced embryos• Ovum Pick Up• Embryo sexing/genotyping• Embryo multiplication• Somatic cloning• Superovulation• Embryo transferThese advanced embryo technolo-gies are applied in modern breeding programs to take full advantage of genomic information. They also can be used to establish new breeding herds in developing countries and to improve local breeds by impor-ting and trasferring selected embr-yo genotypes of the desired breeds.

Equine assisted reproduction

• Ovum Pick Up – ICSI• In vitro embryo production• Stallion fertility test• Embryo freezing• Embryo transfer• Donors boarding service • Recipients serviceAssisted reproduction can overco-me most mare and stallion fertility problems and seasonal limitations: OPU-ICSI can be performed in any period of the year, in any stage of the reproductive cycle with no interferen-ce with training/sporting activities of the donor mares. Embryos can be safelyfrozen for later transfer.

Customized animal modelsUnder research collaborative agreements to develop pigmodels of human diseases and advanced cell therapies

AVANTEA srl - Laboratory of Reproductive TechnologiesVia Porcellasco, 7 /F 26100 Cremona, Italy - tel +39 0372 437242 fax +39 0372 436133 - [email protected] www.avantea.it

Training in in vitro embryo technologies for bovine/buffaloTransfer of knowledgeTechnology Licensing

Services available under specific agreements

ZACTRAN®, EPRINEX®, ANAFEN®, CYSTORELIN®, JVAC® and IMRAB® are registered trademarks of Merial Limited.© Copyright 2012, Merial Canada Inc. All rights reserved. IVMD-12-2756-JA

From top to bottom.

The Products you need

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ZACTRAN®, EPRINEX®, ANAFEN®, CYSTORELIN®, JVAC® and IMRAB® are registered trademarks of Merial Limited.© Copyright 2012, Merial Canada Inc. All rights reserved. IVMD-12-2756-JA

From top to bottom.

The Products you need

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20

Symposia

*Further details on the speakers can be found online at www.icar2012.com/symposia

MONDAY JULY 30

Symposia 1 - 413:45 – 16:45

Symposium 1: Stem and Germ Cell Research and its Application Room: East Ballroom CChair: Fulvio Gandolfi, University of Milan, Italy

Speakers: Le Ann Blomberg, Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, U.S. Department of Agriculture-Agricultural Research Service in Beltsville, MD, USA Twenty years of embryonic stem cell research in farm animals

Tiziana A.L. Brevini, University of Milan, ItalyPluripotency network in farm animal embryos and derived cell lines

Toshihiko Ezashi, Division of Animal Sciences & Bond Life Sciences Center, University of Missouri-Columbia, USA. Induced pluripotent stem cells from pigs and other ungulate species: an alternative to embryonic stem cells

Ramiro Alberio, Division of Animal Sciences (School of Biosciences), University of Nottingham, UKRecent advances in stem and germ cell research in pigs

Symposium 2: Cloning Research and its ApplicationRoom: East Meeting Room 8Chair: W. Allan King, University Guelph, CanadaAssociate Chair: Harpreet Kochhar, Canadian Food Inspection Agency, Ottawa, ON Canada

Speakers: Lawrence Smith, Centre de recherche en reproduction animale (CRRA), Faculty of Veterinary Medicine, University of Montréal, Saint-Hyacinthe, QC, Canada. Inefficiency in cloning and its association with epigenetic alterations to imprinted genes

BC Lee, Department of Theriogenology and Biotechnology, College of Veterinary Medicine of Seoul National University, KoreaLessons learned from cloning dogs

Hiroshi Nagashima, Meiji University International Institute for Bio-Resource Research, Tokyo, Japan Improving development of clones

Harland Howard, Animal Biotechnology Interdisciplinary Group (ABIG), FDA’s Center for Veterinary Medicine (CVM) Silver Spring, USA Agency perspectives and regulatory concerns regarding food safety for the use of animal biotechnology

Symposium 3: Oocyte and Embryo Health Room: East Ballroom BChair: Pat Lonergan, University College Dublin, Ireland

Speakers: Pietro Baruselli, Department of Animal Reproduction, College of Veterinary Medicine, São Paulo University. BrazilManipulation of follicle development to ensure optimal oocyte quality and conception rates in cattle

Trudee Fair, University College Dublin, IrelandRole of progesterone in oocyte quality in cattle

Marc-Andre Sirard, Laval University, CanadaFactors affecting the oocyte and embryo transcriptomes

Urban Besenfelder, Institute of Animal Breeding and Genetics, University for Veterinary Medicine Vienna, AustriaRole of the oviduct in early embryo development

21

Symposia (Continued)

Symposium 4: A New Understanding of Sperm Development and Function Room: East Ballroom AChair: Ina Dobrinski, University of Calgary, Canada

Speakers: Alexander Travis, Reproductive Biology & Wildlife Conservation, Cornell University College of Veterinary Medicine, Baker Institute for Animal Health, USAWhat sperm can teach us about energy production

Jacob Thundathil, Department of Production Animal Health, University of Calgary Faculty of Veterinary Medicine, CanadaThe effect of testicular temperature on spermatogenesis and sperm function

Regina Turner. University of Pennsylvania, USATesticular function in aged males

Goro Yosihzaki, Tokyo University of Marine Science and Technology, JapanGerm cell transplantation in fish

TUESDAY JULY 31

Symposia 5 - 813:45 – 16:45

Symposium 5: Cryopreservation of Gametes, Embryos, Organs and Tissues Room: East Ballroom BChair: Andras Dinnyes, Szent Istvan University, Hungary

Speakers: Muhammad Anzar, Agriculture and Agri-Food Canada/University of Saskatchewan, CanadaResponse of mammalian sperm and testicular tissue to the cryopreservation process

Amir Arav, Institute of Animal Science, IsraelDirectional freezing and other innovative methods for sperm, ovarian tissue and organ cryopreservation

Csaba Pribenszky, Szent István University, Faculty of Veterinary Science, Department of Animal Breeding and Genetics, HungaryDevelopment of oocytes and embryos following high pressure treatment and cryopreservation

Tamara Leahy, University of Sydney ,Australia Exploring seminal plasma and its effects on ram sperm characteristics through processing

Symposium 6: Contraception and Suppression of Reproductive FunctionRoom: East Ballroom 8Chair: Shirley Johnston, Found Animals Foundation, USA

Speakers: Margaret Root-Kustritz, University of Minnesota College of Veterinary Medicine, USAEffects of surgical sterilization on canine and feline health and on society

Michael Munks, Integrated Department of Immunology at National Jewish Health, Denver, USADevelopment of a novel approach towards the non-surgical sterilization of male and female domestic animals

Gregory A. Dissen, Oregon Health and Science University – Oregon National Primate Research Center, USATargeted gene silencing to induce permanent sterility

Scott Struthers, Crinetics Pharmaceuticals Inc. in San Diego, USAApproaches to gonadotroph ablation for nonsurgical sterilization

Symposium 7: Genetic Selection and Preservation of Genetic DiversityRoom: East Ballroom CChair: William V. (Bill) Holt, Zoological Society of London, UK

Speakers:Ann Van Zoom, Faculty of Veterinary Medicine, Ghent University, BelgiumBreeding or assisted reproduction? The horse model applied for the conservation of endangered equids

22


Harvey Blackburn, United Sates Dept. Agriculture, USAGenetic selection and preservation of genetic diversity

Curtis Lind, WorldFish Center, Penang, MalaysiaSelective breeding in fish and conservation of genetic resources for aquaculture

Henri Woelders, Wageningen UR Livestock Research, The NetherlandsHow developments in cryobiology, reproductive technologies and conservation genomics could shape gene banking strategies for farm animals

Symposium 8: Advances in the Understanding of Ovarian FunctionRoom: East Ballroom AChair: Bruce Murphy, University of Montréal, Canada

Speakers:Bob Webb, University of Nottingham, UKEnvironmental influences on folliculogenesis

Patricia A. Johnson, Cornell University, USAFollicle selection in avian follicles

Rebecca Robker, Adelaide University, AustraliaProgesterone receptor-mediated control of ovulation

Joy L. Pate, Agricultural Sciences, Penn State University, USANew concepts in luteal function in ruminants

WEDNESDAY AUGUST 1

Symposia 9 - 1213:45 – 16:45

Symposium 9: The Inter-relationship of Productivity and FertilityRoom: East Ballroom BChair: Matt Lucy, University Missouri, USA

Speakers:Claire Wathes, Royal Veterinary College, London, UKBasic mechanisms linking metabolic status to reproductive outcome in the bovine

George Foxcroft, University of Alberta, Canada Reproduction in farm animals in an era of rapid genetic change: Will genetic change outpace our knowledge of physiology?

Bas Kemp, Department of Animal Sciences of Wageningen University, The NetherlandsShould weaning be the start of the reproductive cycle in the hyperprolific sow? A physiological view

Robert Renema, University of Alberta, Canada Effects of genetic selection on reproductive performance in poultry

Symposium 10: Reproductive Toxicology and the EnvironmentRoom: East Ballroom 8Chair: Rao Veeramachaneni, Colorado State University, USA

Speakers:Sally Perreault Darney, Environmental Protection Agency, USAEndocrine disruptors in the world at large: Evidence from toxicity test systems linking impact on humans, wildlife and domesticated animals

M S (Riana) Bornman, University of Pretoria, South AfricaEnvironmental pollutants and disorders of sexual development in humans and wildlife in South Africa: Harbingers of impact on overall health?

Ulf Magnusson, Swedish University of Agricultural Sciences, SwedenEnvironmental endocrine disruptors in farm animal reproduction: Research and reality

Mehmet Uzumcu, Rutgers University, New Jersey, USAEpigenetic mechanisms in the actions of endocrine-disrupting chemicals: Gonadal effects and role in female reproduction

23


Symposium 11: Applied Research in Male ReproductionRoom: East Ballroom AChair: Chis Maxwell, University of Sydney, Australia

Speakers:Xavier Druart, INRA, FranceInteraction of spermatozoa with the female reproductive tract

Massimo Faustini, University of Milan, ItalyEnhancing performance insemination through controlled release of spermatozoa in pigs

Ulrike Taylor, Institute of Farm Animal Genetics, GermanyImpact of nanoparticles on germ cell viability and functionality

Claire Kershaw-Young, University of Sydney, Australia The role of seminal plasma components on sperm function and fertility in domestic ruminants

Symposium 12: Immunology of PregnancyRoom: East Ballroom CChair: Peter Hansen, University of Florida, USA

Speakers:Doug Antczak, Cornell’s Baker Institute for Animal Health, Cornell University, USAT-cell tolerance to the developing equine conceptus

Lilian Oliveira, University of São Paulo, BrazilCharacteristics and functions of macrophages in the endometrium during pregnancy in the cow

C. Tayade, Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, CanadaImmunological mechanisms at the porcine maternal-fetal interface: insights into embryonic mortality

I. Martin Sheldon, Institute of Life Science at the School of Medicine, Swansea University, UKImmunity and inflammation in the uterus

At Merck Animal Health, our veterinarians, scientists and business experts work together in teams centered around significant categories of disease, not just single products. Close cooperation with scientific centers around the world helps us maintain our focus on cutting edge scientific discoveries in the area of animal reproduction. They can be then translated into practical approaches that allow to secure high reproductive efficacy of production animals and successful management of reproduction in companion ones.

More meat and dairy in our diets increases need on producers to keep breeding levels high and predictable. Merck Animal Health’s fertility programs for production animals have several integral elements: • Hormonesthatsynchronizereproductioncycles• Productsthatfacilitateinseminationandoptimize

breeding strategies like embryo transfer• Vaccinesthatprotectagainstinfectionsthataffect

fertility and could pose a threat to human health• Toolsthathelpdiagnoseandtreatuterine

infections in cattle

For cattle, our hormone portfolio includes RECEPTAL(buserelin),andESTRUMATE(cloprostenol).Forswine,PG600(combinationofgonadotropins)promotesamorefertileestrouscycle,andREGUMATE(altrenogest),isanoraltreatmentusedinlargefarmstooptimizethebreeding process.

Ourhormonesareadministeredinparallelwithvaccinations against diseases that affect reproduction.BOVILISBVDforexample,successfully protects cattle from abortions caused bybovineviraldiarrheavirus.Similarly,OVILISToxovaxandOVILISEnzovaxcanpreventabortionscausedbytoxoplasmosisandchlamydophilosisinsheep and goats.

Toreducethemovementofanimalsbetweenfarms, and the spread of undetected disease, artificial insemination is important to producers. Merck Animal Health also helps them build a strongergeneticpoolwithtoolsformoreefficientartificial insemination.

Managingreproductioninhorsesisacomplexprocessthatrequiresexpertknowledge.Ourfertilityproducts help professional stud farms and private individualsoptimizetheirbreedingprograms.

THE SCIENCE OF HEALTHIER ANIMALS

R0109_12.indd 1 5/31/12 10:20 AM

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Information correct as at 04/2012

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CONTACT:Western College of Veterinary Medicine University of Saskatchewan Saskatoon, SaskatchewanTel: 306-966-7447 | [email protected]

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A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVISHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEOSULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN

BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVISHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEOSULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN CAL-NATE 23 DYSTOSEL FLUNIXIN HIBITANE KOPERTOX NEWCELLS OXY LA 300 OXYTOCIN PREDEF REVIBE REVIBE H.E. A180 CALFSPAN DRAXXIN EXCEDE 200 EXCENEL RTU EXCENEL LIQUAMYCIN LA-200 NEO-SULFALYTE OXYMYCINE LA OXYMYCINE LP PEN-AQUEOUS ALVERIN DECTOMAX VALBAZEN ENVIRACOR J-5 NOVODRY PLUS ORBESEAL PIRSUE SPECIAL FORMULA 17900 FORTE SPECTRAMAST DC SPECTRAMAST LC SYNOVEX C SYNOVEX CHOICE SYNOVEX H SYNOVEX PLUS SYNOVEX S CIDR 330 CIDR 1380 FACTREL LUTALYSE BOVI-SHIELD GOLD 5 BOVI-SHIELD GOLD F P5 BOVI-SHIELD GOLD FP 5+L5 BOVI-SHIELD GOLD FP 5+VL5 BOVI-SHIELD IBR/PI3 CALF-GUARD CATTLEMASTER 4+L5 CATTLEMASTER 4+VL5 CATTLEMASTER GOLD FP 5 INFORCE 3 LEPTOFERM 5 ONE SHOT RESVAC 4/SOMUBAC SCOURGUARD 4KC SOMUBAC SPIROVAC TSV-2 ULTRABAC-7/SOMUBAC ULTRACHOICE 7 ULTRACHOICE 8 ULTRACHOICE 8 VIBRIN

PEACEOFMIND

WITH PFIZER’S COMPLETE LINE OF CATTLE PRODUCTS

Pfizer Animal Health welcomes you to the17th International Congress on Animal Reproduction (ICAR 2012)“CELEBRATING THE PAST AND EMBRACING THE FUTURE”!

Don’t miss the chance to attend the Lunch Symposium: “What is new about Uterine Disease in Cattle?”Monday, July 30 at 12:15 PM, Sponsored by Pfizer Animal Health

> Focusing on your worldwww.plp-cattle.ca

7524_Pfizer_icar_pub.indd 1 12-07-18 2:32 PM

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Workshops

MONDAY JULY 30

Workshops 1 - 916:45 – 18:15

Workshop 1 - Reproductive physiology underlying low fertility in lactating dairy cows and current methods for fertility improvement. Milo Wiltbank, University of Wisconsin, USA ROOM: East Ballroom B Reproductive efficiency of lactating dairy cattle is suboptimal, leading to loss of profitability. The reduction in fertility is multifactorial, potentially involving management, nutritional, and physiological factors. This workshop will focus on physiology that underlies the reduction in conception rates in cows bred to estrus. New synchronized ovulation programs have been developed; the underlying physiology and shortcomings will be explored.

Workshop 2 - Equine reproduction Tom Stout, Utrecht University, The Netherlands ROOM: East Meeting Room 8 This workshop will focus on conceptus/endometrium interactions.• Conceptus signal(s) responsible for triggering maternal

recognition of pregnancy• Regulation of prostaglandin synthesis and oxytocin

receptor function• Does the blastocyst capsule contribute more than

physical protection?• Mechanisms or pathways required for successful

implantation• What information has systems biology (the -omics)

provided?

Workshop 3 - In vitro embryo production Osamu Dochi, Rakuno Gakuen University, JapanROOM: East Ballroom A

This workshop will focus on improving in vitro embryo production efficiency:• Fertilization rates differ among individual bulls

• Media composition affects embryo development and quality

• Cryopreservation of embryos (pregnancy rate)• Oocyte collection by OPU• In vitro embryo production using sexed semen

Workshop 4 - Buffalo (Bubulus) reproduction Nasim Ahmad, University of Veterinary & Animal Sciences, PakistanROOM: East Meeting Room 10

The following topics will be explored:• Delayed puberty (heifers and bulls, endocrinology,

nutrition, management, growth)• Long calving interval (suckling, summer anestrous,

estrus detection, low fertility, silent heat, early embryonic loss)

• Cryopreservation and semen quality; parameters for bull selection

• Synchronization of estrus & ovulation• Reproductive biotechnology (embryo transfer, AI, IVF,

IVEP, cloning)

Workshop 5 - Genomic and proteomic tools in reproduction research Claude Robert, University of Laval, Canada ROOM: East Ballroom C

Genomics – Microarrays and RNAseq• Sample processing profoundly impacts results making

comparisons difficult • Experimental design flaws with biological and technical

replication• Data normalization is extremely difficult• Proteomics: 2D gels and mass spectrometry• Is protein requirement a dead end?• Can proteins be efficiently identified?• How to handle post-translational modifications?

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Workshops (Continued)

Workshop 6 - Emerging roles of seminal plasma Puttaswamy Manjunath, University of Montréal, Canada ROOM: East Meeting Room 12 Seminal plasma is a complex mixture of secretions from the testes, epididymides and accessory sex glands. While it is believed to play a primary role as the carrier of spermatozoa to the female reproductive tract, it also contains proteins which are beneficial (promote motility, capacitation, acrosome reaction, and play a role in sperm reservoir formation in the oviduct) or detrimental (particularly to sperm storage in liquid or frozen state). In recent years, several proteins have been characterized and their physiologic significance has been studied in depth. This workshop highlights emerging roles of major proteins of seminal plasma.

Workshop 7 - Biostatistics George Seidel Jr., Colorado State University, USAROOM: East Meeting Room 7

This Workshop will consider approaches for the following topics:• How to define and analyze pulse data, e.g. LH pulses.• How to choose methods of analyzing binomial data, e.g.

pregnant/not pregnant; fertilized / not fertilized.• Receiver Operator Characteristics/curves that help

define thresholds that minimize both false positives and false negatives (e.g. what concentration of progesterone is indicative of a functional CL).

• Characteristics of competitive fertilization data and analyzing such data.

Workshop 8 - Wild ungulate reproduction Rob McCorkell, University of Calgary, CanadaROOM: East Meeting Room 13

The wild ungulates of Canada live in a temperate climate where adapting their reproductive effort to the seasons is essential for their survival. Therefore, this workshop will focus on the effect of season on reproduction.• Species differences with some discussion about recent

studies involving reindeer• The different responses to season by males and females

of the same species• The effect of season on ovarian follicle development• The effect of season on spermatogenesis

• What sheep can tell us about the effect of season on wild ungulates

Workshop 9 - Tools to study reproductive behaviour Rodolfo Ungerfeld, Facultad de Veterinaria, Uruguay ROOM: East Meeting Room 14 The workshop aims to discuss different strategies to study reproductive behaviour, and the advantages of using reproductive behaviour as a tool to be included in other studies. As a non-invasive technique, the study of reproductive behaviour provides important information on physiological status of experimental units, homogeneity of the population used for research, and information on differences among populations of the same species. The study of sexual behaviour may also provide important clues to improve management, select animals, and improve welfare status. Examples are how social hierarchies may affect cyclic activity, pregnancy outcomes, or the response to biotechnologies. Conversely, socio-sexual stimuli may induce reproductive activity in inactive individuals. A well known example is the use of the male effect, widely studied in sheep, goats and swine, but also applicable in cattle.

WEDNESDAY AUGUST 1

Workshops 10 - 1816:45 – 18:15

Workshop 10 - Use of eCG in reproductive management of beef and dairy cattle Gabriel Bo, IRAC, Argentina ROOM: East Ballroom C In the cow, eCG may demonstrate either FSH or LH activity. eCG has been shown to improve follicular development and ovulation of a larger follicle resulting in a larger, more functional CL, which has resulted in improved pregnancy rates in anestrous beef cows, pasture managed dairy cows and embryo transfer recipients. More recently, eCG given after breeding reduced embryonic loss in dairy cows and in suckled beef cows in fair to poor body condition. With conception and pregnancy rates continuing to decrease globally, this workshop will explore strategies to utilize the potential benefits of eCG in improving bovine reproduction.

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Workshop 11 - Small ruminant reproduction: Clean green and ethical (CGE) management in the developing world? Graeme Martin, University of Western Australia, Australia ROOM: East Meeting Room 8 The CGE concept is driven by consumer demand in high-profit markets for food that is: 1) Clean – food must be safe (no chemicals, pathogens) and healthy (eg, ‘healthy fats’); 2) Green – low environmental impact; 3) Ethical – attention on animal welfare. Importantly, CGE management practices are not expensive, difficult, or ‘high tech’; it simply involves application of basic knowledge of reproductive physiology and behaviour. We will explore the possibilities for CGE practices in the developing world. For example: the ‘male effect’ to control the timing of reproduction; ‘focus feeding’ to improve reproductive outcomes; forages that are anthelmintic and reduce methane production; the power of genetics.

Workshop 12 - Canine and feline reproductionStefano Romagnoli, University of PadovaROOM: East Meeting Room 10

Workshop 13 - Camelid reproduction Marcelo Ratto, University Austral de Chile, Chile ROOM: East Meeting Room 7

The following topics will be explored:• Functional and molecular characterization of an ovulation-

inducing factor present in the seminal plasma of llamas. • Recent advances in semen manipulation and

cryopreservation in South American camelids• Oocyte collection and in vitro maturation in alpacas• The most relevant reproductive disorders present in

alpaca farms in the USA

Workshop 14 - Sperm and embryo sexingSimon de Graaf, University of Sydney, AustraliaROOM: East Ballroom B

Sperm sexing by flow cytometry has produced millions of pre-sexed offspring and remains the only method available for the sex preselection of mammalian offspring. We will explore issues relevant to research and commercial applications, with particular emphasis on cattle.

• The current status of sperm sexing and reasons behind compromised fertility and differences between species

• Commercial aspects of sexed sperm production such as sperm quality and ‘reverse sorting’

• Ideas for improving current methods of sperm sexing, and• Recent technological developments and future research

goals in the field.

Workshop 15 - Imaging in reproductionHeinrich Bollwein, Clinic for Cattle, GermanyROOM: East Meeting Room 12

The introduction of sonography represents a technological breakthrough that has provided novel knowledge about reproductive biology, especially relating to ovarian, uterine and fetal function. To improve objectivity computer-assisted software has been used for analysis of B-Mode images. The development of Doppler processing extended the scope of sonographic imaging from an anatomic to a physiologic basis, and three-dimensional ultrasound imaging allows for the determination of the volume of corpus luteum and follicles and the evaluation of the fetal development. In this workshop these techniques will be reviewed and future developments, possibilities and limitations will be discussed.

Workshop 16 - Reproductive pathologyDon Schlafer, Cornell University, USAROOM: East Meeting Room 14

Endometritis - Overview and update• Role of endometritis in female subfertility (by species)• Uterine pathogens (viral, bacterial, yeast) and their

identification• Comparison and characterization of host tissue (uterine)

responses - gross and histopathology• Endometritis with no demonstrable cause - possible

explanations• Endometrial biopsy: Histopathology: interpretation;

formal “grading schemes”; prognostic value • Discussion of needed research, experimental

approaches, etc.

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Workshop 17 - Swine reproductionNicoline Soede, Wageningen University, The Netherlands ROOM: East Meeting Room 13 Although sustained selection for larger litters has been quite successful, it has changed both the physiology and management of modern, highly prolific sows. This workshop will focus on these changes, during pregnancy (numbers of embryos, pre-natal mortality, pre-natal development), lactation (piglet survival and development, sow energy balance) and post-lactation (decreased subsequent fertility) and the associated changes in management (nutrient requirements, changed weaning management, etc.). The audience is asked to participate in defining the future challenges for on farm reproductive performance and, based on those challenges, discuss directions/challenges for reproductive research.

Workshop 18 - Diagnostic tools for gametes and embryosDon Rieger, LifeGlobal, CanadaROOM: East Ballroom A Gametes and embryos are often evaluated morphologically; however, this is unreliable to determine if a particular oocyte or embryo will result in a successful pregnancy. Measurement of metabolic activity of single embryos to evaluate viability has had contradictory results, but metabolomics and amino acid metabolism have been promising. Increasing evidence suggests that the developmental potential of the embryo is largely determined by the health of the oocyte, including ability to form gap junctional complexes between cumulus cells and expression of specific genes by follicular cells. Two approaches to sperm selection - hyaluronan binding and morphological selection under high magnification are useful for production of viable human embryos using ICSI.

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Poster Presentations

All Paper and e-Poster presentations will take place in East Exhibit Hall B according the schedule below. Electronic posters will be displayed in the order they are listed in the program within each session. Five minutes will be allowed to each presentation. Remember that posters within sections will be available at the corresponding monitor throughout the day.

Poster Session 1Date: Monday, July 30Time: 11:00 - 12:15

Poster Session 2Date: Tuesday, July 31Time: 11:00 - 12:15

Poster Session 3Date: Wednesday, August 1Time: 11:00 - 12:15

SESSION I – MONDAY, JULY 30, 2012

e-Poster PresentationsMonitor 1 – Reproductive behavior

2500: Influence of Bull Exposure on Resumption of Ovulatory Activity of Postpartum Anoestrous Dairy CowsMat, Khairiyah

2502: Sexual and social behaviour signs during the oestrus cycle in dairy cowsBerglund, Britt

2503: Vomeronasal organ blocking and its influence on sexual behavior of Nellore males raised on pastureSilva Moura, Guilherme

2504: Sexual Preference of Ewes for Rams Medicated with NaloxoneBernal, Adriana

2505: Some Factors that Affect the Pregnancy Rate in AlpacasCordero, Aida

2506: Effect of lactation and boar exposure during early lactation on expression of oestrus in multiparous sowsWeaver, Alice

2507: Boar contact is an effective stimulant of lactation oestrusTerry, Robyn

Monitor 1 – Nutrition and reproduction

2000: Concentrations of enterolactone in plasma and pre-ovulatory follicles of dairy cows supplemented with flaxseed and the correlation with intra-follicular estradiol concentrationsZachut, Maya

2001: The impact of maternal nutrition during pregnancy on fetal calf growth and muscle gene expression in beef cattleParadis, Francois

2002: The effects of high and low protein diet intake on the mRNA abundance of mid-luteal phase endometrial candidate genes and ovarian dynamics in high producing lactating dairy cowsIyathurai, Gunaretnam

2003: Effect of undernutrition on embryo production and quality in superovulated ewesAbecia, José-Alfonso

2004: Maternal undernutrition of recipient ewes reduces pregnancy rate regardless level of nutrition of donor ewesMeikle, Ana

2005: Effect of short-term energy supplementation during estrus synchronization on circulating insulin and glucose concentrations and ovulation in Toggenburg goatsBrandao, Felipe

2006: Foetal life protein restriction of mink dams affects the birth weight and pre-weaning growth of their offspringMatthiesen, Connie

Monitor 2 – Folliculogenesis, oogenesis, ovulation - ruminants

1400: Equine Chorionic Gonadotropin (eCG) increases Prolactin receptor (PRLR) expression in the bovine corpus luteumFatima, Luciana

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Poster Presentations (Continued)

1401: Three-dimensional modeling of color Doppler ultrasound images: a new approach to the evaluation of follicular vascularization in cattleArashiro, Eduardo Kenji Nunes

1402: Ovulatory follicle in lactating dairy cows: size matters?Colazo, Marcos

1403: Evaluation of ovarian follicle wave dynamics in the post partum period and until the second ovulation occurrence in Sarda cowsMarongiu, Maria Laura

1404: Repeatability of Dominant Ovulatory Follicle and Corpus Luteum Diameters in Beef CattleFonseca, Gabriela

1405: Changes in expression and localization of cellular flice-like inhibitory protein (cFLIP), an anti-apoptotic factor, in corpora lutea during estrus cycle and pregnancy in Thai Swamp Buffalo (Bubalus bubalis)Wongpanit, Kannika

1406: Predicting the values of oocyte reserves by Antral Follicle Count in buffalo ovariesRiaz, Amjad

1407: Ultrastructural evaluation of buffalo ovarian folliclesOba, Eunice

1408: Identification of the FecL fecundity major gene controlling prolificacy in the Lacaune sheepMansanet, Camille

1409: Determination of Anti-Müllerian hormone plasma concentration can help to select donor sheep for laparoscopic ovum pick-up (LOPU)Lahoz, Belén

1410: Screening of functional shRNA molecules to knock down the expression of ovine BMPR-1B geneHuang, Juncheng

1411: Antimullerian Hormone (AMH) as an Age-Related Predictor of the Antral Follicle Count (AFC) and the Response to Exogenous FSH Ovarian Reserve Test (EFORT) in SheepGonzalez-Bulnes, Antonio

1412: Ovarian follicular dynamics around estrus in Beetal and Teddy goatsRiaz, Hasan

1413: Removal of the Pineal Gland Modifies Folliclle and Corpus Luteum (CL) Growth Dynamics in SheepBerlinguer, Fiammetta

1414: Relationship between pre-ovulatory follicle diameter and corpus luteum size and function after ovulation induction with GnRH or ovulation–inducing factor (OIF) in llamasSilva, Mauricio

Monitor 3 – Transgenesis and cloning

3400: The expression of pluripotency markers in donor cells increases gene expression variability in bovine cloned embryosRodriguez-Alvarez, Lleretny

3401: The aggregation of male cloned embryos with presumptive female in vitro fertilized embryos increases the fetal survival of predominately male chimaeras in cattleWells, David

3402: Production of embryos cloned from beef purchased at the marketSaeki, Kazuhiro

3403: Embryonic patterning and extra-embryonic differentiation appear disconnected before implantation in bovine somatic cell nuclear transfer embryosHue, Isabelle

3404: Scriptaid improves in vitro development of nuclear transfer bovine embryos but not early pregnancy rates in Nelore breedSiqueira Filho, Emivaldo

3405: Dissecting functional oocyte enucleation by actinomycin D for bovine somatic cell nuclear transferMoura, Marcelo

33


3406: Transmission and expression of a retrovirally introduced red fluorescent protein gene in successive generations of transgenic pigsKanai, Takahiro

3407: Effect of Scriptaid treatment on the development of interorder porcine/bovine SCNT embryosOhlweiler, Lain Uriel

3408: in vitro development of intergeneric porcine/bovine SCNT embryos produced from distinct donor cell types of endangered swine breedsMezzalira, Joana Claudia

3409: Porcine transgenesis by intracytoplasmic injection of oolema vesicles co incubated with transgeneLuchetti, Carolina Griselda

3410: β-actin is an optimal reference gene for gene expression analysis of mouse nuclear transfer preimplantation embryoLi, Xiangping

3411: The effect of aurintricarboxylic acid on the intergration of exogenous DNA with Sperm-mediated gene transfer in miceAssumpção, Mayra Elena Ortiz

3412: Generation of transgenic buffalo offspring through lentivirus injectionShi, Deshun

Monitor 4 – Reproductive immunology & toxicology

2600: Relationship between uterine immune function and embryonic mortality in a group of subfertile beef heifersBeltman, Marijke E.

2601: Early postnatal immunisation with a novel gonadotrophin-releasing hormone (GnRH) construct has longer reproductive effects in male than female calvesHernandez-Medrano, Juan H.

2602: Treatment with seminal plasma (SP) modulates the endometrial cytokine network and improves fertility in dairy cowsKatagiri, Seiji

2603: Participation of CD4(+)CD25(+)T cell and WC1(+)γδT cell in pregnancy establishment of cowsMatsui, Motozumi

2604: Effects of prostaglandin F2α on subclinical endometritis and fertility of lactating dairy cows subjected to timed AILima, Fabio

2605: Exposure of pregnant ewes to a complex mixture of environmental chemicals disturbs ovarian development in their offspringCotinot, Corinne

2606: The locally cervical administrated of Misoprostrol or Follicle stimulating hormone (FSH) increase the mRNA expression of beta defensin 2 in the does (Capra hircus) cervical tissue during the estrous cycleIntrakamhaeng, Manakant

2607: The effect of immune modulators on endometrial cytokine expression in mares susceptible to persistent breeding induced endometritis.Woodward, Elizabeth

2608: Low levels of the Herbicide Atrazine contaminated sow feed and water affect litter size, sex ratio of litter and increase mummified fetuses in pigsAm-in, Nutthee

2609: Squamous metaplasia of the prostate and diffuse alopecia in a 13-year-old castrated dog due to chronic ingestion of exogenous estradiolVan den Berghe, Femke

2610: Post-testicular adverse effects after short-term exposure to methylmercury in ratsKempinas, Wilma

34


2611: Effects of Vitamin C on Oxidative Stress Induced in Rat Testis by BTS AntennaJelodar, Gholamali

2612: The effect of probiotic inovo injection in broiler breeding eggs on immune response of their broilerZarei, Abolfazl

Monitor 5 – Oocyte development

2100: Standardization of RNA extraction from bovine oocytesLemes, Rafaella Curvelano

2101: Transcript and protein stockpiles, lipid content and ultrastructure of bovine oocytes enclosed or not within cumulus cells are differently affected by in vitro maturation: implications for developmental competence.Uzbekova, Svetlana

2102: Follicle size of origin affects glucose 6 phosphate dehydrogenase activity and developmental competence of oocytes from Boran cowsMuasa, Bridgit

2103: Organelle Modulation in Bovine Oocytes During Dominant Follicle Growth and MaturationDadarwal, D.

2104: Comparison between different maturation systems acting through the manipulation of cAMP dependent pathways in bovine oocytesMarelli, Elisa

2105: Synchronization of follicular development does not improve ovum pick up yield in Bos taurus indicus cattleCabral de Almeida, Rafael Vinícius

2106: Recombinant bovine somatotropin administration improves ovum pick up yield in Nelore (Bos taurus indicus) cattleOliveira, Maira Souza

2107: Mitochondria differ between adult and prepubertal sheep oocytes during in vitro maturationReader, Karen

2108: CD9 Expression by Ovine Oocytes and Its role in Sperm-Oocyte InteractionsNason, Narenhuar

2109: Oocyte Mitochondrial Bioenergy Potential and Oxidative Stress in Adult Superovulated Ewes: Within-/Between-Subject, In Vivo versus In Vitro Maturation and Age-Related VariationsLacalandra, Giovanni Michele

2110: Factors affecting in vitro maturation of alpaca oocytesMesserschmidt, Chelsey

2111: Developmental competence of equine oocytes after chemical or mechanical activation and assisted fertilization (ICSI).Kochan, Joanna

2112: WTAP gene expression in in vivo and in vitro porcine oocytesPark, Kwang-Wook

2113: Different media used for selection of competent swine oocytes with Brilliant Cresyl Blue stainingSantos, Elisa Caroline da Silva

2114: Effects of Oxygen Concentration on In vitro Maturation of Canine OocytesSalavati, Mazdak

Monitor 6 – Testis - spermatogenesis

3300: The Role of Testicular Biopsy and Ultrasonography in Diagnosis of Infertility in Camels (Camelus dromedarius)Waheed Abdelrahman, Magdi

3302: Structural and functional proteins are differentially expressed in morphologically abnormal bovine spermKastelic, John

3303: Expression of anti-Müllerian hormone, cyclin kinase inhibitor, connexin 43, androgen receptor and steroidogenic enzymes in the normal and cryptorchid equine testisBall, Barry

35


3304: The effect of mildronate on morphology of testes, biochemical blood indices, sexual reflexes and semen quality in boarsAntane, Vita

3305: Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoaYi, Young-Joo

3306: Proteomic analysis of sperm anterior head plasma membrane: unfolding the molecular components underlying sperm-egg interactionKongmanas, Kessiri

3307: Morphological and ultrastructural assessment of giant panda spermatozoaZhou, Luyun

3308: Testicular weight and its correlation with body weight and testicular biometry in Japanese quail: implications for selection of breedersMarques Junior, Antonio

3309: Replacement of soybean oil for flaxseed oil in the diet of Rhamdia quelen males: effects on sperm motility and membrane integrityBongalhardo, Denise

3310: Changes in notch signaling pathway expression in the testis are associated with spermatogenesis along the mouse post-natal lifeMurta, Daniel

3311: Comparison of 2D and 3D culture systems for murine male germ cell differentiationLandim Alvarenga, Fernanda da Cruz

3312: Characterization of a novel calcium binding protein of sperm :CABS2Kawashima, AKihiro

3313: Effect of an Injectable Mineral Solution on Lipid Peroxidation and Semen Quality in Young Brahman BullsFlores Solano, Celeste

Monitor 7 – Cryopreservation of semen

0600: Evaluation of epididymal bovine sperm after post mortem short-term storage at 5ÂºCCardoso, Rita

0601: Mass Spectrometric plasma lipid fingerprinting kynetics after birth in calves as a tool to track metabolic changes in the extra uterine life adaptationCosta Simas, Rosineide

0602: Studies on assessment of optimal osmotic pressure of citrate egg yolk extender for cryopreservation of buffalo bull semenPasha, Talat Naseer

0603: Capacitation status and fertilization ability of thawed ram sperm obtained with artificial vagina (AV) and electroejaculation (EE)Alberio, Ricardo

0604: Influence of cholesterol-loaded cyclodextrin pretreatment on osmotic tolerance of ram spermatozoaAhmad, Ejaz

0605: Efficacy of low density lipoproteins in replacement of whole egg yolk in a commercial extender “Botu Bov® “ for the preservation of motility and membrane integrity of frozen-thawed ovine spermHenry, Marc

0606: The cryoprotective effects of soybean lecithin-based extender on goat sperm qualityGuerra, Maria Madalena Pessoa

0607: Storage of porcine semen at 4°C without antibiotics preserves semen motility and results in similar pregnancy rates, embryo development and litter size.van Leeuwen, J

0608: Methylformamide and glycerol as successful combination for canine semen cryopreservationHoogewijs, Maarten

36


0609: The effect of suprazero and subzero freezing rates on lipid peroxidation in post-thaw rhesus macaque spermatozoaMartorana, Kelly

Monitor 8 – Female reproductive tract - nonruminants

1300: Influence of the level and duration of local inflammatory reaction of mare’s uterus to the systemic plasma concentrations of acute phase proteins.Kavak, Ants

1301: Diestrus oxytocin administration prolongs luteal function and decreases endometrial cyclooxygenase-2 expression in maresKeith, Lauren

1302: Estrogen alpha and progesterone receptors in endometrial immune cells of mares with endometritis, effect of immunomodulation.Fumuso, Elida

1304: Nodal and receptors Alk4 and Alk7 in equine corpus luteum: mRNA quantification and functional roleGalvão, António

1305: Influence of endometrial condition of mares on expression of LactoferrinUrayama, Shuntaro

1306: Sow oviduct and uterus myoelectrical activity during early pregnancy in pigs using telemetry implantsGajewski, Zdzislaw

1307: Interactions of spermatozoa and uterine epithelial cells in the pig: a cell culture studyBergmann, Annabel

1308: Expression and localization of key elements of microRNA processing machinery in the porcine endometrium during early pregnancyKaczmarek, Monika M.

1309: Is insulin a signal molecule regulating corpus luteum life span in the bitch?Papa, Paula

1310: Expression of oviductin mRNA in the canine oviduct during the periovulatory periodTahir, Muhammad Zahid

1311: Novel links between shell gland functions and phytoestrogenWistedt, Anna

2212: Activin A during Thoroughbred pregnancy and immunohistochemical localization of its receptors and intracellular mediators in equine fetal gonadsDhakal, Pramod

Monitor 9 – Estrus synchronization

1100: Pregnancy rates in Bos indicus crossbred suckled cows treated with a progesterone releasing device, estradiol and inseminated at 48 or 54 h after device removalCutaia, L

1101: Effect of progesterone content in a releasing device on the time of ovulation in Bos indicus crossbred suckled cows.Videla Dorna, Ignacio

1102: Serum progesterone concentrations, corpus luteum development and follicular dynamics after administration of equine chorionic gonadotrophin (eCG) at the end of a synchronization protocol for fixed-time AI in anestrous beef cattle.Núñez, Richard

1103: Effect of Testosterone on Dominant Follicle Growth and Luteal Dynamics in Beef HeifersAl-Shanoon, Hayder

1104: Addition of eCG on day 5 instead of day 7 of modified HeatSynch protocol might improve the ovarian follicular response of the anestrus suckled Hereford cowsCavestany, Daniel

37


1105: Mutations in genes involved in the oestrous cycle associated with expression of oestrus in cattleHomer, Elizabeth

1106: Efficiency of combined protocol for re-insemination of open cows after pregnancy diagnosis on day 20 post insemination and its effect on fertilityGaja, Abdurraouf

1107: Comparison of the proportion of Bos indicus heifers pregnant following synchronisation of ovulation and fixed-time AI or oestrous detection before and after a single prostaglandin F_{2α}_ treatmentEdwards, Sophia

1108: Development of the ‘sMartwin’ synchronization protocol for beef cattle twinningMartinez, Marcelo

1109: Follicular wave synchronization with estradiol benzoate in Gyr (Bos indicus) cattleSinedino, Letícia

1110: Size of the dominant follicle at prostaglandins application in onset of estrus and fertility of Holstein cowsRodríguez, Raymundo

1111: Comparison of three estrus synchronization methods on estrus response and conception rates in buffaloes.Ahmad, Nasim

1112: Reproductive performance of Egyptian Baladi goats following induction of estrus using prostaglandin F2αEl-Amrawi, Gamal

1113: Comparison of two commercially available progestagen intravaginal inserts in a short-term protocol for estrus synchronization in cyclic ewesFleisch, Andreas

1114: Short and long protocols for estrus synchronization and timed-AI in Dorper and White Dorper ewesde Souza Traldi, Anneliese

1115: Human chorionic gonadotropin and gonadotropin releasing factor agonist induce ovulation in the mare with the same high efficiencyBarreto Filho, Joao Bosco

1116: Evaluation of novel protocols for ovulation induction in the mareGreco, Gabriel Maksoud

Monitor 10 – Puberty & neuroendocrine regulation of reproduction

2400: Puberty and sexual maturity in Senepol bulls raised semi-intensively in Central BrazilAndrade, Venicio-Jose

2401: FSH polymorphism as a molecular marker of sexual precocity in Nellore bullsCipriano, Vivian Taís Fernandes

2402: Decrease in progesterone concentration was not followed by an increase in plasma luteinizing hormone in Nellore calves (Bos taurus indicus) after birthNogueira, Guilherme

2403: The effects of a soybean and canola diet on sexual maturation in dairy heifersBarrett, David

2404: Expression of kisspeptin neurons in the arcuate nucleus of the goat during the follicular and luteal phases– A preliminary studyZamiri, Mohammad Javad

2405: Transrectal ultrasonography in measurement of uterine diameter in ewe lambs after progestagens stimulus.Monteiro Toma, Claudia Dias

38


2406: Effect of melatonin treatment on plasma IGF-I level, and gene expression of β-lactalbumin, BCL2, BAX, and BCLX in the mammary epithelium in sheepFaigl, Vera

2407: Glutamatergic receptors are expressed at pronounced higher rate in the ovine fetal hypothalamus than pituitary during the late gestation periodRabaglino, Maria

2408: Serum Levels of Free Thyroid Hormones and Cortisol During the Mare’ Oestrus CycleLacalandra, Giovanni Michele

2409: Preliminary assessment of the relation between luteinizing hormone and progesterone concentrations on day 14 after insemination in sowsSoede, Nicoline

2410: Evaluation of the reproductive potential of 1 year old female Tasmanian devils and their prospective contribution to the captive breeding programKeeley, Tamara

Paper Poster PresentationsPoster Board #10650: Evaluation of in vitro fertilizing capacity of cryopreserved bull epididymal semenMoreira da Silva, Fernando

Poster Board #20651: Using of Low Density Lipoproteins and Glutamine to Improve Frozen Buffalo Bull Semen and FertilityEl-Sharawy, Mohamed

Poster Board #30652: Evaluation of frozen-thawed Girolando bull semen diluted with commercial extender and rediluted or not with powder coconut water based solution (PCW-111^®: in vitro and in vivo resultsMachado, Vanessa

Poster Board #40653: Effects of storage and whole or clarified egg yolk in a commercial semen extender on cooled preservation of Pelibuey hair ram (Ovis aries) semenDominguez-Rebolledo, Alvaro

Poster Board #50654: Effect of combined addition of BSA and glutathione to semen extender on quality and fertility of boar semen stored at 17˚CErokhin, Anatoly

Poster Board #60655: Critical time-points in canine semen cryopreservationLucio, Cristina

Poster Board #70656: Evaluation of the use of Low-Density Lipoproteins (LDL) extenders in the cryopreservation of electroejaculated semen from brown bear (Ursus arctos)Anel, Luis

Poster Board #80657: Conservation of genetic potentials of Eastern Sarus Crane (Grus antigone sharpii): effects of season and preservation techniques on semen qualityTharasanit, Theerawat

Poster Board #90658: Effects of alternative cryoprotectants, diluents, straw size and cholesterol addition on cryopreserved rooster spermTarvis, K.M.

Poster Board #100659: The effect of omega-3 fatty acids addition in sperm extenders on the quality of frozen bovine semenAbavisani, Abbas

Poster Board #110660: Field fertility results of sublethal stress treated frozen-thawed boar semen using commercial cervical insemination without ovulation inductionPribenszky, Csaba

Poster Board #121150: Use of a deslorelin implant to induce estrus in coyotes (Canis latrans)Christensen, Bruce W.

Poster Board #131151: Integral Reproduction Program On Captive Bottlenose Dolphins with Assisted Reproduction Techniques Developed In DolphinarisUgaz, Cristian

Poster Board #141152: Timed artificial insemination with sex-sorted semen in synchronized and re-synchronized estrous cycles in Nelore cowsNeves, Kedson

39


Poster Board #151350: Uterine cellular immune response after artificial insemination in maresFioratti, Eduardo

Poster Board #161351: Mares with intense uterine degenerative changes have lower luteal vascularity and plasma progesterone concentration during the early gestationCamargo Ferreira, Jair

Poster Board #171352: Identification of Interstitial Cajal-Like Cells in the Sow Uterus and OviductGajewski, Zdzislaw

Poster Board #181450: Histological characteristics of luteal structures in dairy cowsBalogh, Orsolya Gabriella

Poster Board #191451: Effect of peptidoglycan (PGN) on steroid production in bovine theca cellsHoriuchi, Maya

Poster Board #201452: Study of Gene Expression in Granulosa Cells from Different Follicle Growth Phases in relation to Bovine Oocyte QualityDouville, Gabriel

Poster Board #211453: MicroRNA expression during ovarian follicular differentiationDonadeu, F. Xavier

Poster Board #221454: Ovarian follicle count and plasma AMH concentration on Nelore and Angus heifersEreno, Ronaldo Luiz

Poster Board #232051: Correlations of zinc and selenium concentrations in seminal plasma and serum with sperm count and morphology in domestic catsS B Villaverde, Ana Izabel

Poster Board #242052: Effect of dietary energy on sperm membrane integrity and seminal plasma protein concentrations in mature ramsGuan, Yongjuan

Poster Board #252053: Oral L-arginine supplementation increases vascular perfusion of the ovulatory follicle in post-partum maresKelley, Dale

Poster Board #262150: Thermoprotective effects of IGF-I on bovine germinal vesicle oocytesPaula-Lopes, Fabíola F.

Poster Board #272450: A comparison of puberty traits in angus, brahman, hereford, and senepol heifersWarnick, Alvin C

Poster Board #282451: Detection by Immunohistochemistry of the N-Methyl-D-aspartate receptor subunit-1 (NMDAR-1) in the intact sheep hypothalamusRuiz, Ana

Poster Board #512453: The Relationship Between the Hormones Involved in Growth and Reproduction in Trichogaster Trichopterus Male - A Model for the Suborder of Anabantoidei FishDegani, Gad

Poster Board #522452: Effects of melatonin on salsolinol-induced prolactin secretion in goatsHashizume, Tsutomu

Poster Board #292454: Development of specific immunoassay systems for canine pituitary hormones using hetero-antibody fluor-immunoassay (FIA) methodHasegawa, Yoshihisa

Poster Board #302550: Lack of a relationship between social rank and reproductive patterns in semicaptive pampas deer (Ozotoceros bezoarticus) malesUngerfeld, Rodolfo

Poster Board #312551: Reproductive hormone patterns of pregnant, pseudo-pregnant and acyclic captive African wild dogs (Lycaon pictus)Martin, Graeme B.

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Poster Board #322552: Reproductive Problems in Landhi Buffalo Colony, Karachi, PakistanFareed, Syed Khurram

Poster Board #332050: Short term dietary propylene glycol supplementation affects circulating metabolic hormones and progesterone concentrations and follicular growth in dairy heifersPonsart, Claire

Poster Board #342151: Identification of deadenylated RNAs in bovine immature oocytesGohin, Maella

Poster Board #352152: Effects of FGF10 on oocyte maturation, quality and capacity to become an embryoCastilho, Anthony Cesar Souza

Poster Board #362153: FSH/LH, PKC and EGF involvement in bovine cumulus cell expansion during oocyte maturationLopes, Everton

Poster Board #372154: Effect of inhibin A on the expression of Brain-Derived Neurophic Factor (BDNF) in porcine granulosa cells and the expansion of cumulus oocyte complexes (COCs) in vitroWang, Chunqiang

Poster Board #382155: Effects of Bcl-2 inhibitor (ABT-737) treatment on developmental competence, apoptosis and ER- stress in pigsKoo, Deog-Bon

Poster Board #392156: The beneficial effect of t10,c12–conjugated linoleic acid on in vitro maturation of porcine oocyteZhu, Shi-En

Poster Board #402158: Immunolocalization of GDF9 and BMP15 during in vitro maturation of canine oocytesDe los Reyes, Monica

Poster Board #412159: Juxtacrine and paracrine mutual oocytes-granulosa cells interaction: a proposed modelSaadeldin, Islam M.

Poster Board #422650: A bovine serocolostrum to transfer the passive immunity in foals and calvesJimenez-Lopez, Anne J. E.

Poster Board #432651: Expression of the cytokine IL-23 in gilt endometrium after insemination with seminal plasma, spermatozoa or semen extenderDalin, Anne-Marie

Poster Board #442652: The Effect of Black seed (Nigella sativa) Extract on Gonadotropin, Estrogen, Progesterone and Folliculogenesis in MicePoor Naji, Narges

Poster Board #453350: In Vitro culture of equine testicle samplesMartin, Ian

Poster Board #463450: Comparison of transfection methods effectiveness used to obtain transgenic pigs for xenotransplantationJura, Jacek

Poster Board #473451: In vitro development of CMV-Fut transgenic and non-transgenic pig embryoSmorag, Zdzislaw

Poster Board #483452: Gene Expressed in Ram Spermatid after Delivered into Germ CellsCao, Wenguang

Poster Board #493453: A cloned camel (Camelus dromedarius) calf produced from the skin fibroblasts of an elite bullWani, Nisar Ahmad

Poster Board #503454: Outgrowths and capsule derived from aggregated cloned equine embryosGambini, Andres

41


SESSION II – TUESDAY, JULY 31, 2012

e-Poster Presentations

Monitor 1 – Male reproductive tract

1900: Epididymosomes transfer Epididymal Sperm Binding Protein 1 (ELSPBP1) to dead spermatozoa during epididymal maturationD’Amours, Olivier

1901: Incorporation of various dietary omega-3 fatty acids into bull sperm and the effects on physiological parameters of fresh and frozen-thawed spermZeron, Yoel

1902: Recommended minimum scrotal circumference for tropically-adapted beef bulls in northern AustraliaMcGowan, Michael

1903: Ultrasonography of the scrotal contents for breeding zebu bulls extensively reared in Costa RicaChacon, Jorge

1904: Thermographic monitoring of scrotal surface during GnRH test in young bulls with low semen qualityVencato, Juri

1905: Echographic assessment of scrotal contents in extensively reared bullsVinoles, Carolina

1906: Immunohistochemical localization of aromatase in goat testis and accessory glands.Vaziry, Asaad

1907: Major proteins of the ram seminal plasma: attributes and interaction with the sperm membraneMoura, Arlindo

1908: Colour-coded and pulsed Doppler measurements of testicular arterial blood flow in male Donkey (Equus Asinus).Zelli, Riccardo

1910: Identification of Matrix metalloproteinase (MMP-2, MMP-9) and their cognitive inhibitors (TIMP-1, TIMP-2) in the male canine reproductive systemWarinrak, Chuleekorn

1911: Morphometric studies of the testicles of Savanna Brown bucks raised in local farm settlements within a typical middle belt area of NigeriaFasanya, O. O. A.

Monitor 2 – Folliculogenesis, oogenesis, ovulation - nonruminants

1500: Granulosa cell mRNA expression of hormone receptors and enzymes and follicle blood flow in maresWischral, Aurea

1501: Morphologicc development and morphometry of the germinative cells of equine foetal ovariesBastos, Henrique Boll de Araujo

1502: Anti-Mullerian Hormone (AMH): expression and possible roles in the porcine ovaryAlmeida, Fernanda

1503: Prolactin, andostenedione and IGF1 serum concentrations during induced follicular growth by eCG administration in the bitchStornelli, Maria Alejandra

1504: Ovarian Activity during the Estrous cycle and the Pregnancy in Asian elephants (Elephas maximus)Lueders, Imke

Monitor 2 – Animal models for biomedical research

0100: Effectiveness of exogenous surfactant treatment to preterm newborn lambs.Silva, Liege

0101: Synthesis of testosterone and sulfonated steroids in the porcine testicular-epididymal compartment: need for concept revision?Hoffmann, Bernd

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0102: Expression pattern of transgene in cloned and recloned transgenic dogsOH, Hyun Ju

0103: Evaluation of placental vascularization using 3D Power Doppler angiography and stereology in a rabbit model of IUGRChavatte-Palmer, Pascale

0104: The intake of green tea does not interfere in the number of corpora lutea in superovulated rats.Giometti, Ines Cristina

Monitor 3 – Genetics, genomics and epigenomics

1700: Extensive Alterations in the Quantitative Proteome Profile during Early Embryonic Development in CattleArnold, Georg J.

1701: MiR-218 modulates early lineage segregation in the bovine blastocyst by targeting CDH2 and NANOGGoossens, Karen

1703: Identification and analysis of beef heifers with superior capacity for fertilityMinten, Megan A.

1704: HDAC inhibition decreases transcription of imprinted genes during early embryo development in cattleRizos, Dimitrios

1708: Copy number variation of testis specific protein Y-encoded (TSPY) gene is associated with fertility in bullsKing, W Allan

1711: Evidence for a single gene affecting embryo/fetal survival in the Davisdale flockJuengel, Jenny

1712: Methylation change of imprinted genes (IGF2 and H19) in serial recloned pigletHoon, Jang

1713: Partial reprogramming of imprinted gene expression during post-implantation development of mouse embryos exposed to TSA but not 5AzaC during pre-implantationBaqir, Senan

1714: Chimeric RNA is an important mode of gene transcription in mammalXie, Bingkun

Monitor 3 – Genetics, genomics and epigenomics

1702: TET gene family transcript profiles during bovine embryo pre-implantation stagesBurroughs, Chelsie

1705: Trichostatin-A Increase Levels of Cytosine 5-Hydroxymethylation in Bovine CellsPerecin, Felipe

1706: Across-species analysis of gene expression changes in the endometrium during the pre-implantation phaseBauersachs, Stefan

1707: In silico study of pattern of CpG islands in Bos taurus genomeMioranza, Anderson

1709: Effects of Lactation on Gene Expression of Endometrium of Holstein Cows at Day 17 of the Estrous Cycle or PregnancyCerri, Ronaldo

1710: Two novel mutations in the Bone Morphogenetic Protein 15 gene are associated with increased ovulation rate, but no sterility, in the French Grivette and the Polish Olkuska breeds of sheepFabre, Stephane

1715: RNAseq analysis identifies global gene expression changes during rhesus macaque (Macaca mulatta) oocyte maturationMeyers, Stuart A.

Monitor 4 – Epidemiology, reproductive diseases & pathology I

0900: Reproductive diseases survey in small herds assigned to set up artificial insemination programs in São Paulo State, BrazilAlvarez, Rafael H

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0901: Isolation of mycoplasma species from the uterus of early postpartum dairy cows with and without endometritis and its association with dystociaOsawa, Takeshi

0902: Prevalence of subclinical mastitis in dairy farms in urban and peri-urban areas of Kampala, UgandaAbrahmsén, Markus

0903: Effect of mastitis during the first lactation on necessity to cull of Holstein cowsHeravi Moussavi, Alireza

0904: Detection of Neospora caninum in some cases of unexplained ewe abortions in the north east of IranAsadpour, Reza

0905: Seroreactivity to leptospirosis and reproductive failure in mares embryo recipients in Rio de Janeiro, Brazil.Pinna, Aline

0906: Leptospirosis by serovars Bratislava and Copenhageni and reproductive failures in mares in Rio de Janeiro, BrazilLilenbaum, Walter

0907: Microbiological and molecular Lactobacillus spp. detection in mare vaginal samplesCastro-Chaves, Manoela

0908: Prevalence of virulence factor genes of E. coli is associated to the different outcomes of uterine infection in the bovine and canine speciesMateus, Luisa

Monitor 5 – Early embryonic development

0700: Effect of bovine oviductal epithelial cell co-culture on early cleavage kinetics of bovine IVP embryosCordova, Amanda

0701: Linoleic acid incorporation and competence of bovine oocytes matured in vitroCarro, Mercedes

0702: Prostaglandin and progesterone production by bovine embryos and co-cultured luteal cells in two in vitro culture systemsLopes-da-Costa, Luís

0703: Molecular mediators of conceptus-maternal crosstalk during bovine maternal recognition of pregnancyLonergan, Patrick

0704: Genomic action of thyroid hormones during bovine early embryo development in vitroAshkar, Fazl A.

0705: The effects of saturated fatty acids on bovine oocyte developmental competenceAardema, Hilde

0706: RNA-Seq profiling of bovine preimplantation embryosWolf, Eckhard

0707: Exogenous melatonin improves ovine embryo viability in undernourished ewes during the breeding seasonVázquez, M. Isabel

0708: Comparison of homologous versus heterologous fetal fibroblasts to support goat embryo attachment in vitroLam, Louise

0709: Fusion of cytoplasmic fragments to porcine oocytes improves the ability for embryonic developmentKikuchi, Kazuhiro

0710: Diffusion Influences in Vitro Mammalian Embryo Culture: A Numerical CalculationMatsuura, Koji

0711: Use of a mechanical vibration system to observe the effects of mechanical stimuli on in vitro mouse embryo developmentAsano, Yuka

0712: Rhesus macaque embryo development and gene expression is altered by oxidative damage to the fertilizing spermKlooster, Katie

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Monitor 6 – Spermatology & andrology I

2900: Fertility prediction in buffalo bulls on the basis of sperm motion traits, fertility associated antigen (FAA) and HSP70 expressionKumar, Ajeet

2901: Exploring in vitro sperm characteristics and their importance in the prediction of conception rateOliveira, Leticia

2902: Seminal plasma proteins from Dairy Gir bulls in peripuberty and their relationship with sexual precocity and fertilityVale-Filho, Vicente

2903: Composition and digestibility of dietary mineral supplementation increase the semen quality of A.I. bullsScholz, Heiko

2904: Season-induced variations in lipid composition are associated with semen quality in Holstein bullsRoth, Zvi

2905: Effect of Vitamin E on epididymal sperm motility in water buffalo (Bubalus bubalis) post-mortemAbdy, Keivan

2906: Causes of rejection of rams in the breeding soundness evaluationMenegassi, Silvio Renato Oliveira

2907: First Report of Dietary L-carnitine and Fish Oil Effects on Sperm Characteristics in RamAlizadeh, AliReza

2908: The effects of oxytocin and PgF2alpha injections on libido sexualis and semen quality in warmblood stallionsAkcay, Ergun

2909: Impact of computer-assisted sperm morphology analysis for quality assurance of AI centersWaberski, Dagmar

2910: Separation of apoptotic boar spermatozoa using magnetic activated cell sorting with annexin V-conjugated microbedsZrimšek, Petra

2911: Sequence of tyrosine phosphorylation pattern during boar sperm capacitation in vitroBergqvist, Ann-Sofi

2913: DNA damage assessment of canine freeze–dried sperm using Acridine Orange and alkaline Comet AssayOña Magalhães, Luis Carlos

2914: Different separation methods for removal of blood from dog semen - preliminary studyPrinosilova, Petra

Monitor 7 – Cryopreservation of oocytes & embryos

0500: Pregnancy rates following transfer of bovine embryos crypreserved in ethylene glycol for Direct Transfer or vitrified using CryotopsDochi, Osamu

0501: Supplementation of maturation medium with L-carnitine improves developmental competence of in vitro matured bovine oocytes after vitrificationChankitisakul, Vibuntita

0502: Comparison of vitrification and slow controlled freezing protocols for the cryopreservation of bovine blastocystsAchilles, Marcos

0503: In-straw cryoprotectant dilution of IVP bovine embryos vitrified using a solid-surface systemBó, Gabriel A.

0504: Effect of Trehalose Supplementation in Vitrification Medium on Developmental Potential of Vitrified Ovine OocytesSuccu, Sara

0505: Catalase prevents lipid peroxidation and maintains survival of caprine preantral follicles cryopreserved in a 1,2-propanediol-freezing mediumRodrigues, Ana Paula

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0506: Apoptotic changes in equine embryos vitrified by two different methodsOkolski, Adam

0507: In vitro evaluation of equine embryo viability after 6 or 24 hours of cooling in different mediaPanzani, Duccio

0508: Vitrification of GV and IVM Horse Oocytes with Two Different Equilibration MethodsRosati, Irma

0509: Hollow fiber vitrification (HFV) method enables cryopreservation of cryosensitive porcine embryosMatsunari, Hitomi

0510: Short-term storage of collared peccaries (Tayassu tajacu) ovarian tissue using phosphate buffered saline (PBS) solution or powdered coconut water (ACP®) based mediumSilva, Alexandre R

0511: Ultrastructure of fresh and frozen ovarian tissue in doe rabbit: effect of different combinations of cryoprotective agent and sugarNeto, Vanessa

0512: Vitrification of immature bovine cumulus-oocyte-complexes using natural honey as a non-permeant cryoprotectantAlfoteisy, Bilal

Monitor 8 – Female reproductive tract - ruminants

1200: Evaluation of uterotonic effects of Dinoprost in pyometric and clinically healthy lactating dairy cowsMasoumi, Reza

1201: The Sensa cow, a useful step between two dimensional pictures plus slaughterhouse material and in vivo clinical practice on transrectal explorationJonker, Herman

1202: Selected amino acid transporters are altered in the bovine endometrium and conceptus during the pre-implantation period of pregnancy and are modulated by altered progesterone concentrations in vivoForde, Niamh

1203: Inhibin alpha subunit induces apoptosis of bovine granulosa cells via Bcl-2 familyLiguo, Yang

1204: Low insulin-like growth factor-1 concentrations before parturition are associated with the development of cytological endometritis in multiparous dairy cowsWaldmann, Andres

1205: Functional and structural changes in bovine corpus luteum during partial luteolysisTrevisol, Eduardo

1206: Effects of mating, artificial insemination or intravaginal deposition of raw semen or seminal plasma on vaginal and uterine blood flow in German Holstein cowsBollwein, Heinrich

1207: Oxylipidomics of the cow uterus: effects of the peri-ovulatory endocrine milieu of the estrous cycleBertan Membrive, Claudia

1208: Gene expression of glucose transporters in cattle endometrium in response to manipulations of the pre-ovulatory follicle sizeRodrigues Franca, Moana

1209: The peri-ovulatory endocrine millieu regulates the expression of polyamine synthetic pathway in the bovine endometriumBinelli, Mario

1210: Prostaglandin E1 analogue (PGE1) and follicle stimulating hormone (FSH) increase the expression of FSH receptor in the cervix of goats (Capra hircus) during the estrous cycleLeethongdee, Sukanya

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1211: Intracervical administration of follicle stimulating hormone (FSH) or prostaglandin E1 (PGE1) induces expression of EP1 and EP2 receptors in the cervix of Thai goats (Capra hircus) during oestrusPholseang, Chatsumal

1212: Transient post-mating uterine inflammation in the alpacasSumar, Julio

1213: Fate of the unfertilized ova in alpacasPicha, Yessenia

Monitor 9 – Artificial insemination

0200: Differences in age at first calving as a result of various usage of sex sorted semenJoezy-Shekalgorabi, Sahereh

0201: Reproductive performance in beef heifers supplemented with rumen-protected fat prior or after fixed-time AIBorges, Batista Souza, Joao Batista

0202: Incorporation of Sexed Semen into Reproductive Management of Range Cow-Calf OperationsCooke, Reinaldo

0203: Effect of GnRH on conception rate of heifers and suckling beef cows not in estrus at fixed time AIDick, Alberto

0204: Use of eCG or hCG on day 7 after FTAI to improve pregnancy rate in beef cowsGonsioroski, Andressa Varella

0205: Progesterone supplementation improves fertility in lactating dairy cows without corpus luteum at the initiation of the 5-d timed AI protocolBisinotto, Rafael

0206: A 50-day period of negative energy had no effect on pregnancy per artificial insemination in heifersParr, Mervyn

0208: Pregnancy rates in hair sheep ewes following vaginal insemination with fresh and short-term stored liquid semenWildeus, Stephan

0209: Oral vitamin B1 supplementation prior to laparoscopic artificial insemination improves pregnancy and lambing rates in Pelibuey ewesGomez Gonzalez, Arturo

0210: Fixed-time Artificial Insemination in AlpacasBravo, Walter

0211: Fertilization rate and number of embryos in sows reproductive tracts after intra-uterine insemination with cryopreserved boar semen supplemented with seminal plasmaChanapiwat, Panida

0212: Effect of semen extender on frozen-thawed sperm characteristics and distribution in the female genital tract after deep intrauterine insemination in pigsNoguchi, Michiko

Monitor 10 – Pregnancy & placenta physiology

2300: Environmental regulation of Pregnancy-specific protein B concentrations during late pregnancy in dairy cattleThompson, Izabella

2301: The use of 3D ultrasonography for determination of fetal gender in the bovine fetus during early pregnancyPinto, Carlos

2302: Digital rectal palpation: a technique for gynaecological examination and pregnancy diagnosis in red sokoto goatBello, Adeh Awak

2303: Activation of the Fibroblast Growth Factor (FGF)-system during pre-implantation equine pregnancyStout, Tom

2304: Identification of the gp130 receptor family genes and their expression in the endometrium and conceptus during implantation period in maresHaneda, Shingo

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2305: MicroRNA biogenesis-associated genes expression and miRNA expression profile in porcine embryos.Krawczynski, Kamil

2354: Visualisation of pregnancy in the Tammar wallaby Macropus eugeniiDrews, Barbara

2800: Nutritional supplementation with 140g of mesquite together with the male effect improve reproductive performance of anoestrous ewesMuñoz-Gutiérrez, Minerva

2801: Reproductive variables of Murrah Buffaloes according to different level of milk productionBartolomeu, Claudio Coutinho

Monitor 10 – Reproductive management - Other ruminants & pigs

2802: Number of fetuses influences the accuracy of pregnancy scanning in ewesSöderquist, Lennart

2803: Flushing with extruded of soybean and crude glycerine increased the percentage of twins in ewes under extensive system conditionsPérez-Clariget, Raquel

2804: Adding lupins to pre-mating diets improved potential litter size of gilts mated during the seasonal infertility periodvan Wettere, William

2805: Effects of pre-mating conditions on subsequent piglet birth weight and uniformityWientjes, JGM

2806: Lactation weight loss in mildly feed restricted sows and its relation with embryonic survival and metabolic blood parameters during lactation and gestationKemp, Bas

2807: Seasonal influence on the culling pattern of gilts and sows in ThailandTummaruk, Padet

2808: Development of species-specific vaccines for population control of wild pigs using recombinant phageSamoylova, Tatiana

2809: Effect of egg weight on embryonic development and hatchability of Naked neck chickensAlabi, Olushola John

Paper Poster PresentationsPoster Board #10150: Production of transgenic pig harboring an endothelial cell-specific human CD73 expression cassette by nuclear transferOh, Keon B

Poster Board #20151: DNA methylation changes in oocytes and embryos during mouse agingFu, Xiang-wei

Poster Board #30152: Generation and characterization of induced pluripotent stem cells (iPS) from adult canine fibroblastsPiedrahita, Jorge

Poster Board #40153: In vivo heat stress affects development of preimplantational rabbit embryosLopez-Bejar, Manel

Poster Board #50251: Reproductive Efficiency of Holstein Cows Submitted to Fixed Time Artificial Insemination in Semi-Arid RegionsInocencio Guido, Sebastião

Poster Board #60252: Cows diagnosed with ovarian cysts derive no benefit from progesterone supplementation but conceive normally to a GnRH-based timed A.I. protocolAmbrose, Divakar J.

Poster Board #70253: Genetic and environment factors as leverage effects on fertility at first service in Holstein cows together with milk production and insemination conditionsPonsart-Posiere, Claire

Poster Board #80254: Reproductive outcome of prostaglandin based protocol for timed artificial insemination (TAI) in ewes: effects of focus feeding and GnRH treatmentGil, Jorge

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Poster Board #90255: Vaginal mucus and hyperemia affects fertility of artificial insemination in Assaf breed sheepAlvarez, Maria Mercedes

Poster Board #100550: Effect of hyperbaric oxygen therapy on the follicular reserve of canine ovarian tissues after xenotransplantationSuzuki, Hiroshi

Poster Board #110551: Optimization of a warming and dilution protocol for modified open pulled straw vitrification of murine blastocyst stage embryosCabrera, Pedro

Poster Board #120552: Improvement of new vitrification method for mouse preimplantation embryosKong, I.K.

Poster Board #130553: The exposure of bovine immature oocytes to “Nitrocooler” negative pressure enhances cryotolerance of in vitro produced blastocystsMezzalira, Alceu

Poster Board #140554: Cryopreservation of doe rabbit ovarian tissue: toxicity of freezing solutions and effect of post-seeding freezing rate on the morphology and on the DNA fragmentation of preantral folliclesJoly, Thierry

Poster Board #150750: Ovulatory response and embryonic development in superovulated lactating holstein cows supplemented with trans–10, cis–12 conjugated linoleic acid in the dietCarvalhais, Isabel

Poster Board #160751: Effect of treatment of bovine blastocysts with colony stimulating factor 2 during the morula-blastocyst stages of development on growth and gene expression of trophectoderm outgrowthsDobbs, Kyle

Poster Board #170753: FGF signalling in the trophoblast precedes conceptus elongation in the pigValdez-Magana, Griselda

Poster Board #180754: Eld’s deer embryos produced in vitro can develop to term after transfer into recipient femalesSiriaroonrat, Boripat

Poster Board #190755: Role of hyaluronic acid in maturation and further early embryo development of bovine oocytesFouladi-Nashta, Ali A.

Poster Board #200756: Rescuing the development competence of poor quality oocytes by mitochondrial transferLee, Hoon Taek

Poster Board #210757: Addition of caffeine on embryonic development of Danio Rerio Rodrigues-Ferreira, Mônica

Poster Board #220950: Evaluation of cumulus-oocyte complexes and follicular fluid harvested from Bovine Herpes Positive Cattle following administration of dexamethasoneOliveira, Andre Penido

Poster Board #230951: Evaluation of Bovine Spermatozoa following exposure to Bovine Herpesvirus Type 1 (BoHV-1)Goncalves, Roseli

Poster Board #240952: Factors effecting conception rate in pasture-fed dairy cowsMcDougall, Scott

Poster Board #250953: Identification of toxic shock syndrome toxin-1 (TSST-1) gene of Staphylococcus aureus isolated from bovine mastitis milk in Urmia, IranAhmadi, Malahat

Poster Board #261250: Interaction between Neurotrophin 4 and gonadotrophins in bovine ovaryZhou, Xu

Poster Board #271251: Bovine seminal plasma synchronizes ovulation in heifersTribulo, Paula

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Poster Board #281252: Effect of semen quality on cow uterine artery resistance index by color Doppler ultrasound after artificial inseminationArruda, Rubens Paes

Poster Board #291550: In vitro culture of equine preantral follicles obtained via Biopsy Pick-Up methodHaag, Keith

Poster Board #301551: Intrafollicular haemorrhage induced by meloxicam and phenylbutazone administration in mares: preliminary dataMartins, Carla

Poster Board #311552: Bcl-2 members, Bid and Bax, are involved in granulosa cell apoptosis in sawsManabe, Noboru

Poster Board #321553: Morphological and molecular assessment of capuchin monkey ovarian tissue following exposure to vitrification solutions: Trolox supplementation improves follicular survivalSilva de Lima, Julianne

Poster Board #331555: In vitro development of preantral follicles from capuchin monkeys (Cebus apella)F S Domingues, Sheyla

Poster Board #341750: Microarray analysis of single bovine embryoLandim-Alvarenga, F.C.

Poster Board #351751: Accelerated genetic gain by embryo genotyping with the Illumina Bovine SNP50 BeadchipVigneault, Christian

Poster Board #361752: A comprehensive survey of genetic variation of Italian sheep breeds at 50K SNP loci: implications for breed management and conservationPassero, Adriano

Poster Board #371753: Using metabolomics to non-invasively assess bovine embryo viability in vitroMadan, Pavneesh

Poster Board #381754: Transcriptome changes during bovine blastocyst formation in response to culture environmentTesfaye, Dawit

Poster Board #391950: Intratesticular injection of zinc gluconate as a permanent contraceptive for catsOliveira, Erika C. S.

Poster Board #402350: Use of an ELISA for serum PSPB in dairy cows with twin or single fetusesGábor, György

Poster Board #412351: Conceptus development and global gene expression at preimplantation stages in lactating dairy cows of distinct genetic groups and estrous cyclic statusesRibeiro, Eduardo

Poster Board #422352: Analysis of perinatal uterine artery and umbilical cord Doppler velocimetry in sheepVeiga, Gisele

Poster Board #432353: Metalloproteinase 2 (MMP-2), osteopontin (OPN) and leukemia inhibitory factor (LIF) genes expression in the oviduct and uterus of pregnant and nonpregnant bitchesPagnano Derussi, Ana Augusta

Poster Board #442355: Early detection of dystocia in Holstein cows using plasma pregnancy- specific protein B (PSPB), progesterone and some hematological and blood biochemical characteristicsEidan, Sajeda

oster Board #452356: Altering rumen degradable to undegradable protein ratio: Influence on nutrient consumption, blood urea nitrogen, involution of uterus, ovarian cyclicity and pregnancy rate in Nili-Ravi buffaloesSarwar, Muhammad

Poster Board #462358: Serum Biomarkers of Health in Pregnant Buffaloes During Last TrimesterRahman, Zia

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Poster Board #472850: Species variations in success of treatment of endometritis and conception rates following infusion of Lugole’s iodine in buffalo (Bubalus bubalis) and cowsBrar, P.S.

Poster Board #482851: Male goat testosterone secretion is stimulated in February-April after exposure to only 1.5 months of long days in DecemberDelgadillo, José Alberto

Poster Board #492852: Improving the artificial insemination management in Mediterranean sheep: a field trial in Comisana and Massese breedsGalli, Andrea

Poster Board #502853: Estrogen and progesterone receptors in the vagina of cycling ewes and anoestrous ewes trated with GnRH with or without progesterone primingTasende, Celia

Poster Board #512854: Effect of Feed Supplementation on Conception Rate and Embryonic Survival in AlpacasHuanca, Wilfredo

PPoster Board #522855: The effect of a low prolactin dose administered per os on mortality and body gain of pigletsGajda, Barbara

Poster Board #532856: Conditioning Murrah buffalo heifers prior to application of Ovsynch protocol improves the treatment response and fertilityKumaresan, A.

Poster Board #542857: Follicular wave emergence after GnRH induced ovulation at the beginning of the breeding season in dromedaries (Camelus dromedarius)Nagy, Peter

Poster Board #552858: Follicular arrest in ovaries induced by Busereline in alpacas (Lama pacos)Echevarria, Luisa

Poster Board #562859: Characterization of follicular dynamics and corresponding hormone concentrations in multi- and nulli-parous mithuns (Bos frontalis)Mondal, Mohan

Poster Board #572950: No link between sperm morphology and velocity across maternal mouthbrooding cichlid fish from Lake MalawiSimpson, Julia

Poster Board #582951: Effect of melatonin implants on scrotal circumference and seminal quality parameters of dohne merino rams during seasonal anestrus at 43˚sBuffoni, Andres

Poster Board #592952: Effect of adding soybean or salmon oil and vitamin E the diet on the ultrastructure of boar spermatozoa after cryopreservationAndrade Moraes, Elenice

Poster Board #602953: Effect of milk extender supplemeted with vitamin E analog in chilled canine semenVicente, Wilter

SESSION III – WEDNESDAY, AUGUST 1, 2012

e-Poster Presentations

Monitor 1 – Stem cell biology in reproduction

3100: Bovine endometrial cells express markers of pluripotence and display osteogenic and chondrogenic differentiation potential in vitroCastro, Fidel Ovidio

3101: Long-term Culture and Identification of Bovine Spermatogonial Stem CellsWu, Yingji

3102: The role of GalNAc glycan residues associated adhesion and self-renewal in cultured bovine gonocytes.Kim, Sung-Min

51


3103: Isolation and chondrogenic differentiation of bone marrow mesenchymal stem cells (MSC) from bovine fetusesPeralta, Oscar

3104: Analysis and comparison of the expression of SOX2 in blastocyst and hatched blastocystda Silva Rascado, Tatiana

3105: Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetusesNagao, Yoshikazu

3106: Induced pluripotent stem cells derived from equine keratinocytesSharma, Ruchi

3107: Cultivation of mesenchymal stromal cells from cryopreserved mononuclear cells isolated from equine umbilical cord bloodDe Schauwer, Catharina

3108: Plasticity in pig induced pluripotent stem cells modulated by small moleculesAlberio, Ramiro

Monitor 2 – Other

3500: Effects of by pass fat supplementation on the productive and reproductive performance of first-calf Brahman cowsHernandez, Rolando

3501: Intergenerational fetal programming effects on lamb growthPeterson, Sam

3502: Investigation of the presence of adhesin genes cna, fnbA and fnbB amongStaphylococcus aureus isolated from clinical and subclinical sheep mastitis in IranDastmalchi Saei, Habib

3503: Seasonal and ecological variations in the serum steroid hormone concentrations of one-humped male camel in PakistanPasha, Riaz Hussain

3504: Sperm factors influencing sow insemination outcomesMcPherson, Francoise

3505: Administration of 4-vinyl-1-cyclohexene 1,2-epoxide diminishes ovarian follicles in dogsPaksoy, Zahid

3506: Therapeutic ultrasound as a potential male dog contraceptive: comparison of application protocolsLeoci, Raffaella

3507: Verification of a bioassay for measuring luteinising hormone (LH) in the Asian elephant (Elephas maximus)Nice, Penny

3508: Semen characteristics and libido of rabbit bucks fed diets containing Garcinia kola seed mealHerbert, Udo

3509: Extensive Review of Assisted Reproductive Technologies for Amphibian ConservationZahedi Abdi, Alireza

3510: Clinical Evaluation of non-surgical sterilization of male dogs with a single intra-testicular injection of a new drug Chemisterisol™Jana, Kuladip

Monitor 3 – Conservation of genetic- and bio-diversity

0300: Monitoring salivary progesterone and estradiol in a captive female Amazonian manatee (Trichechus inunguis)Amaral, Rodrigo S.

0301: Effect of chilling duration on post-thaw characteristics of sperm from the north american bison (bison bison)Dance, Alysha

0302: Effects of reproductive seasonality on gamete quality in Bison bison bisonThundathil, Jacob

0303: Assessment of sperm morphology of tambaqui (Colossoma macropomum)Oberst, Eneder

52


Monitor 3 – Genetic improvement

1601: Evidence that total number of ovarian follicles ≥ 3 mm in diameter is moderately heritable in dairy cowsWalsh, Siobhán W.

1602: Polymorphisms of 4 Microsatellite Loci and the Association with Caprine Litter SizeHua, Guohua

1603: Association of osteopontin polymorphisms with placental efficiency and reproductive performance in Lezhi Black goatZhao, Yongju

Monitor 3 – Stress, Nutrition and reproduction

3200: Hair cortisol concentrations and semen production of Bos taurus bullsPeric, Tanja

3202: Effect of rumen-inert fat enriched with polyunsaturated fatty acids (Ω–3 and Ω–6) on the reproductive and productive performance of crossbred dairy cowsDíaz, Thaís

3203: Incorporation of various dietary omega-3 fatty acids into granulosa cells and oocytes and the effects on in-vitro fertilization performance in dairy cowsMoallem, Uzi

3204: Involvement of free cholesterol and high density lipoprotein in development and resistance of the preimplantation bovine embryo to heat shockMoss, James

3205: Follicular Environment and Embryo Development of Dairy Cows Fed Diet Containing Low Concentration of Fatty Acids Supplemented with Different Sources of Fatty AcidsGreco, Leandro

3206: The vaginal prostaglandin E (PGE) or Hyaluronan (HA) base impregnated sponges did not alter the serum Malondialdehyde concentration in Thai goat (Capra hircus) during the oestrusPhimphanit, Winai

3207: When two rat litters – in utero and suckling – are overlapped, the second litter tends to be smaller, but newborn pups have bigger body and testicular weight and higher Sertoli cell numbersBielli, Alejandro

Monitor 4 – Epidemiology, reproductive diseases & pathology II

1000: The developmental ability of cattle oocytes is impaired in animals with liver disorderSarentonglaga, Borjigin

1001: Clinical mastitis is not associated with induction of luteal regression in dairy cowsWolfenson, David

1002: Incidence of cytological endometritis and its effects on reproductive performance of crossbred dairy cowsSantos, Ricarda M.

1003: The effects of dystocia on production, reproduction and health of dairy cattleFarzaneh, Nima

1004: Post-operative fertility following Caesarian Section in cows - A 12 year retrospective studySharma, Arvind

1005: Relationship Between Cyclical Changes in Endometrial Cytology and the Diagnosis of Subclinical Endometritis in CowsMadoz, Laura

1006: A retrospective study of treatment of Trichomonosis in bullsIrons, Peter

1007: Aspermatogenesis and hypospermatogenesis in culled Sahel goats in NigeriaAbba, Yusuf

1008: Puncture of epididymis in boars as a procedure in diagnostics of porcine brucellosisRogozarski, Dragan

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Monitor 5 – IVF/ICSI/IVP

1800: Developmental rates of IVP bovine embryos using minigradient of Percoll®, Isolate® and Optiprep®Pegoraro, Lígia Margareth Cantarelli

1801: Atp content and gene expression profile in cultured bovine morula resulting from high glucose exposure before compactionSirard, Marc-André

1802: Pregnancies after transfer of bovine embryos cultured in a novel microfluidic device applied with a trap and release systemImai, Kei

1803: In vitro production of Mediterranean buffalo embryos using sexed semen results in low fertilization and development ratesGalli, Cesare

1804: Heparin and cumulus cells affect in vitro fertilization and developmental competence of goat oocytesSouza, Joanna M G

1805: The effect of phytoemagglutinin on in vitro embryo development of ovine prepubertal oocytesBogliolo, Luisa

1806: The effect of culture medium changes on in vitro production of sheep embryosVilariño, Marcela

1807: Preliminary comparison between different ovum pick-up intervals and different maturation methodsPalmer, Eric

1808: Assessment of cytoplasmic markers after simulated stress in in vitro maturation of porcine oocytesGonzález, Raquel

1809: Effects of dibutyryl cAMP on ROS and GSH of porcine oocytes, apoptosis of cumulus cells, and embryonic developmentYu, Il-Jeoung

1810: Effects of the zona pellucida on in vitro sperm penetration into porcine oocytesTanihara, Fuminori

1811: Hyaluronan and proteoglycan binding link protein 1 and versican enhance cumulus expansion during in vitro maturation of porcine cumulus oocyte complexesYoshioka, Koji

Monitor 6 – Spermatology & andrology II

3000: Correlation of semen fertility with ROS levels and motility in a multiparametric analysis.Vincent, Patrick

3001: Comparison of fixable and non-fixable fluorescent stains for bovine spermatozoa viability assessmentsBurkhardt de Koivisto, Marion

3002: Content and activity of Na/K-ATPase in high- versus low-fertility holstein bullsRajamanickam, Gayathri

3003: Effects of extender and equilibration time on post-thaw motility of cryopreserved bull semen at 0, 30, 60 and 90 minutes after thawingMontiel Palacios, Felipe

3004: Capacitation affects the location of Na^{+}^/K^{+}^ ATPase in bull spermBuhr, M.M.

3005: Egg yolk-induced head-to-head agglutination of bull spermatozoa is prevented by α-ketoglutarateYang, Danhui

3006: A preliminary assessment of the ram spermatozoa glycoproteome using 2DLC-MS/MSRickard, Jessica P.

3007: Comparison of sperm motility kinetics and membrane phospholipid translocation between cryopreserved ejaculated sperm and epididymal sperm from stallionsMonteiro, Gabriel Augusto

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3008: Influence of time within the breeding season on ROS production and stallion sperm qualityMorrell, Jane

3009: Contribution to assessment of cat kinetics sperm motility using computer-assisted sperm analysisLopes, Maria Denise

3010: The effects of various diluents on sperm motility of rabbit semen stored at 5˚C for up to 96 hoursJohinke, Danielle

Monitor 7 – Cryobiology

0400: Sequestration of BSP-A1/-A2 minimizes cryoinjury to bull spermatozoaSrivastava, SK

0401: Evaluation of lipid composition and localisation with spectrophotometry in single bovine embryosGuignot, Florence

0402: Cryopreservation method affects the extent of apoptosis in trophectoderm and the speed of re-expansion in bovine in vitro produced blastocystsNagai, Takashi

0403: Bovine Embryo Culture In the Presence of Antioxidants: Implications For Blastocysts Development, Cryotolerance and Levels of Intracellular Reactive Oxygen Species (ROS)Rocha, Nathália Alves de Souza

0404: Effects of the use of antioxidants trolox, catalase and glutathione in the extender glycine-yolk-milk with 5% egg yolk on sperm kinetics, membrane integrity and lipoperoxidation in cryopreserved ram semenRodello, Leandro

0405: Gelatin in the cooling medium of ram semenAzevedo, Hymerson Costa

0406: Custom microarray analysis of gene expression of ovine oocytes subjected to vitrificationLedda, Sergio

0407: Use of Collagenase Type-1 to Improve the Seminal Characteristics of Dromedary Camel (Camelus dromedarius) SemenShekher, Chandra

0408: Evaluation of Two Cryopreservation Systems for Asinine SpermOliveira, José Victor

0409: Spatial temporal relationship of mitochondria and lipid droplets of swine embryos may reflect successful embryo cryopreservation for gene bank conservationAmorim, Lincoln

0410: The effect of homologous prostatic fluid on the parameters of dog semen extended and frozen in Tris-egg yolk and INRA Freeze: preliminary resultsStãnescu (Pascal), Manuela

0411: Use of freezing machine (TK3000) on cryopreservation of dog epididymal spermMartins, Maria Isabel

0412: A Novel Method of Seminal Plasma Separation Before Cryopreservation of Stallion SemenRamires Neto, Carlos

0413: A chemically defined substitute to products of animal origin improves viability of in-vitro produced bovine embryos after slow-freezingBuff, Samuel

Monitor 8 – Reproductive management - cattle

2700: Reproductive Performance of Crossbred Dairy Cows under Smallholder Condition in EthiopiaDinka, Hunduma

2701: Transvaginal single needle aspiration: an alternative treatment of ovarian follicular cysts in dairy cows?Parlevliet, Joyce

2702: Effects of bovine somatotropin injection on serum progesterone concentrations in nonlactating dry cowsVasconcelos, Jose Luiz

55


2703: Treatment of repeat breeder cows with intrauterine infusion of platelet rich plasma (PRP)Lange Consiglio, Anna

2704: Reproductive efficiency and possible risk factors for poor reproductive performance in cattle herds bred naturally in the Lake Chad basin of NigeriaMshelia, Gideon

2705: Prepartum supplementation with rice bran and crude glycerine improves the productive and reproductive perfomance of beef cows in pastoral systemPerez Clariget, Raquel

2706: Factors influencing the chance of cows being pregnant 30 days after the voluntary waiting periodLöf, Emma

2707: Effect of age at weaning and plane of nutrition before weaning on progesterone secretion and uterine gene expression at first service in beef heifersViñoles, Carolina

2708: Effect of age at weaning and the plane of nutrition before weaning on growth and somatotropic axis gene expression in the liver of beef heifersGuggeri, Diana

2709: Hormonal and metabolic profile of crossbred Holstein Zebu cows in the transition periodSilper, Bruna

Monitor 9 – Embryo transfer

0800: Superovulatory response of beef cows treated with a split-single injection of Folltropin-V diluted in hyaluronan after storage in the refrigerator or after freezingTríbulo, Andrés

0801: Survival of in vivo-produced bovine embryos exposed to 1.5 M ethylene glycol for different periods of time prior to conventional cryopreservationTribulo, Humberto

0802: Consequences of embryo transfer using in vitro produced embryos for characteristics of the offspring and recipient after calvingBonilla, Luciano

0803: Season and follicular population on the efficiency of an embryo production program in Holstein heifersRangel, Raymundo

0804: PGF2a treatment during the periovulatory period increases embryo recovery rate in superovulated buffaloesBaruselli, Pietro S.

0806: High efficiently reproduce mutton breeding sheep by the embryo transfer technic in the fieldChen, Jingbo

0807: Day of collection affects the number of embryos recovery on ewesSicherle, Carmen

0808: Comparing two superovulation protocols in dromedary camelsNiasari-Naslaji, Amir

0809: Factors Affecting Embryo Collection and Transfer in AlpacasCoutinho da Silva, Marco A.

0810: Superovulation, embryo recovery, and pregnancy rates in seasonally anovulatory mares treated with recombinant equine FSHRoss, Pablo J.

0811: Use of fluorescent stains to assess viability of fresh embryos from superovulated pigsCornejo, Miguel A.

Monitor 10 – Parturition & post-partum physiology

2200: Effects of two different hormonal treatments (progesterone and GnRH+PGF2a) on the dairy cows with postpartum anoestrusHamali, Hossein

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2201: Endocrine profiles during the peripartum of primiparous rangeland beef cows of different body condition score (BCS) at calvingAstessiano, Ana Laura

2202: Gene expression profiles and differentiation in new developing bovine trophoblastic cell linesHashizume, Kazuyoshi

2203: Relationship between acute phase proteins and subsequent fertility of dairy cows after postpartum uterine inflammationKask, Kalle

2204: Use of lectin histochemistry to study the development of the bovine placentome during gestationParkinson, Tim

2205: Induction of parturition in cattle using dexamethasone profoundly suppresses maternal corticosteroid secretion and alters maternal plasma electrolytesLee, Rita

2206: Oxidative stress in Holstein calves born under distinct obstetrical conditionsVannucchi, Camila

2207: The effect of lactation on post partum uterine involution in Holstein dairy cowsScully, Stephanie

2210: Concentrations of copper and its main antagonists in colostrum and serum of Bergamacia sheepToma, Hugo Shisei

2211: Transcervical Administration of PGF2-α Analog to Interrupt Gestation in MaresAguilar, Javier

2213: Cloprostenol-induced changes in endometrial proteins and gene expression during the third week of pregnancy in maresHayes, Tony

2214: Uterine fluids and fertility during the first postpartum ovulatory period in Thoroughbred mares under tropical conditionsQuintero, Beatriz

2215: Foaling characteristics and retained foetal membranes (RFM) in mares conceiving under natural day-length and foaling at pastureEvans, Margaret

2216: Concentration of lactate and cortisol in feline amniotic and allantoic fluids throughout pregnancyRigau, Teresa

Paper Poster PresentationsPoster Board #10350: Presence of MT1 melatonin receptor gene polymorphism in Araucana Creole SheepSepúlveda, Nestor

Poster Board #20450: Osmotic reaction of Jersey dairy cattle embryos exposed to various cryoprotective additivesYoungs, Curtis R.

Poster Board #30451: Aloe vera sp. is an acceptable alternative to egg yolk for preserving goat semen at 4˚CMelo, Cibele C. S.

Poster Board #40452: Vitrification of two–cell mouse embryos has no effect on gene expression and histone marks of H19, Igf2 and Mest genes in blastocyst stageMovaghar, Bahar

Poster Board #50850: Efficacy of hyaluronan as a diluent for a two injection FSH superovulation protocol in Bos taurus beef cowsHockley, Duncan

Poster Board #60851: Superovulatory response in hair ewes following subcutaneous osmotic pump infusion of FSHRamon, Julio

Poster Board #70852: Relationship between ovarian status prior to superovulatory treatment and ovarian response in hair ewesCervera, Deneb

Poster Board #80853: Influence of Season on Superovulatory Response and Embryo Yield of Santa Ines Ewes Submitted to Fish Treatment Started Near the time of Emergence of the First Follicular WaveOliveira, M.E.F.

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Poster Board #90854: Superovulatory responses and embryo recoveries in Rathi (Bos indicus) cattle: prospects and limitationsPurohit, Govind Narayan

Poster Board #101050: Reproductive and productive parameters of Holstein cows presenting normal or pathological puerperiumMaia Borges, Alan

Poster Board #111051: Factors affecting stillbirth and twin birth in Holstein dairy cattle in northeast of IranAzizzadeh, Mohammad

Poster Board #121052: Incidence of cytological endometritis and its effects on reproductive performance in Nelore cowsCarneiro, Luísa

Poster Board #131650: Simulated economic efficiency in a complete cycle production system of beef cattle bulls using in vitro production of embryosGontijo Pellegrino, Carlos Augusto

Poster Board #141651: Detection of polymorphism in Booroola gene (FecB) and its association with litter size in Zel sheep breed in IranJafari Joozani, Razi

Poster Board #151850: Effect of the reproductive status of Crossbred Zebu cows on in vitro embryo productionFernández, Adriana

Poster Board #161851: Improved bovine embryo production by in vitro fertilization with X-sorted sperm using in vivo matured oocytes obtained by follicle superstimulation and ovum pick-upMatoba, Satoko

Poster Board #171852: Effects of brilliant cresyl blue staining on the efficiency of in vitro fertilization and parthenogenetic activation of oocytes in buffalo (Bubalus bubalis)Zhang, Ming

Poster Board #181853: Cumulus Cells are Involved in Oocyte Maturation and Fertilization in In Vitro Produced Ovine EmbryosMenchaca, A.

Poster Board #191854: In vitro development of feline embryos after intracytoplasmic sperm injection with 7 day refrigerated testicular spermBuarpung, Sirirak

Poster Board #201855: Effect of Donor Age on ovum Pick-up results in BuffaloesQin, Guang-sheng

Poster Board #211856: Season effect on Ovum Pick-up oocytes of Water Buffalo with FSH treatmentLiang, Xian-wei

Poster Board #221858: In vitro development and DNA fragmentation of domestic cat ICSI embryos subjected to antioxidant conditions and ionomycin activationSalamone, Daniel

Poster Board #232250: Uterine blood flow at various stages of pregnancy in dairy buffaloesVarughese, Emy Elizabeth

Poster Board #242251: Lung Morphometry of Preterm Puppies after Maternal Betamethasone TreatmentMachado Regazzi, Fernanda

Poster Board #252252: Epithelial cell differentiation regulated by microRNA-200a in mammary glandNagaoka, Kentaro

Poster Board #262253: Effect of passive immunization against inhibin-α subunit and non-steroid bovine follicular fluid (NSBFF) on mammary gland growth and development in primiparous female Wister RatsAl-Sa’aidi, Jabbar

Poster Board #272254: Abortion induced by cloprostenol does not affect the fertility of dairy heifersFernandes, Carlos Antonio

Poster Board #282750: Effect of meloxicam on pregnancy rate of recipient heifers of in vitro produced embryos with different degrees of cervical manipulation difficultyMartins, Lílian

Poster Board #292751: Influence of progesterone and progesterone associated with hCG on pregnancy rate of Nelore cows used as recipient of in vitro produced embryosBalbinot, Matheus

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Poster Board #302752: Effect of estrus synchronization on interval to estrus and pregnancy of bull-bred, post-partum beef cowsGarcia Guerra, Alvaro

Poster Board #312753: Progesterone determination in milk in grazing Holstein cows as a predictive tool for reproductive efficiencyCarriquiry, Mariana

Poster Board #322754: Early weaning in primiparous autumn-calving cows in low body condition score: effects on body condition, nefa and insulin concentrations and ovarian cyclicityQuintans, Graciela

Poster Board #332755: Effect of Body Condition and Supplementation During Postpartum on the Onset of Ovarian Activity and Pregnancy Rate in Crossbred Cows (Bos taurus x Bos indicus) Under Tropical ConditionsDominguez, Carlos

Poster Board #343050: Assessment of Bovine Sperm Capacitation Pathway L-Arginine/NO/GMPC Through in Vitro Embryo ProductionCaldas Bussiere, Maria Clara

Poster Board #353051: Use of in vitro assessed semen quality criteria to predict fertility of bull semenPonsart, Claire

Poster Board #363052: Correlations between testicular hemodynamic and sperm characteristics in ramsFernandes, Claudia Barbosa

Poster Board #373054: Do spermatozoa from fertile Thoroughbred stallions live fast and die young?Gibb, Zamira

Poster Board #383150: Comparison of differential plating methods to isolate bovine spermatogonial stem cellsIanello Giassetti, Mariana

Poster Board #393151: Effect of Different FCS Concentrations on Matured Bovine Spermatogonial Stem Cells Coculture with Sertoli Cells.Ebrahimi, Amin

Poster Board #403152: Treatment of mares with chronic uterine injury using stem cells obtained from bone marrowAlvarenga, Marco Antonio

Poster Board #413153: Isolation and nuclear transfer of germ-line stem-like cells from adult pig testesHan, Yong-Mahn

Poster Board #423250: Influence of two kinds of handling on pregnancy rate and on reactivity score of Nelore cows using fixed time artificial insemination (FTAI)Tirloni, Rayf

Poster Board #433251: Effect of Heat Stress in the Maturation Rate, Fecundation, and Development of Bovine Embryos In Vitro FecundatedD’angelo, Magali

Poster Board #443252: Effect of dam size and nutrition during pregnancy on fetal ovary development of offspringKari, Asmad

Poster Board #453253: Short- or long-term hypobaric hypoxia decreases corpus luteum size and insulin-like growth factors in cycling sheepParraguez, Víctor H.

Poster Board #463254: Reproductive performance of Ouled Djellal ewes in arid area of AlgeriaKahramen, Deghnouche

Poster Board #473255: Effects of nutritional restriction on metabolic and ovarian function in llamasRatto Fuster, Marcelo

Poster Board #483550: Relationship between single nucleotide polymorphisms of growth hormone receptor and reproductive performance in Holstein dairy cowsMunakata, Megumi

Poster Board #493551: High-throughput and multiplexed UPLC-MS/MS quantification of melatonin and its metabolites in bovine ovarian follicular fluid SamplesCoelho, Mirela B.

Poster Board #503552: The use of equine follicle stimulating hormone to increase equine chorionic gonadotropin in the pregnant mareHughes, Sydney

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Ancillary Meetings

SUNDAY, JULY 29

16:30 – 18:00ICAR Executive CommitteeRoom: Pan Pacific Hotel Vancouver – Pacific Rim 1 MONDAY, JULY 30

12:15 - 13:45 ICAR Standing CommitteeRoom: Vancouver Convention Centre – East Meeting Room 12 TUESDAY, JULY 31

10:30 – 12:30IETS MeetingRoom: Vancouver Convention Centre – East Meeting Room 8

WEDNESDAY, AUGUST 1

10:30 – 12:00ECAR / ACTRoom: Vancouver Convention Centre – East Meeting Room 13

11:00 - 12:00 SOLARARoom: Vancouver Convention Centre – East Meeting Room 1-3

12:15 - 13:45ICAR Standing CommitteeRoom: Vancouver Convention Centre – East Meeting Room 12 THURSDAY, AUGUST 2

12:00 - 14:00ICAR Executive CommitteeRoom: Pan Pacific Hotel Vancouver – Pacific Rim 1

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Industry-Sponsored Symposia

MONDAY, JULY 30

12:15 – 13:45East Meeting Rooms 1-3

What is New About Uterine Disease in Cattle?

Prof Martin Sheldon (Swansea University, UK) will describe advances in our understanding of how endometrial pathogenic bacteria cause metritis and endometritis. Dr. Doug Hammon DVM, PhD (Pfizer Animal Health, US) will discuss treatment strategies to reduce the impact of endometrial specific pathogens on uterine disease. At the completion of the two discussions, there will be a ten minute question and answer period. SPONSOR:

TUESDAY, JULY 31


The Future of FSH: Progress Toward a Recombinant Technology

CHAIRPERSON: Dr. Reuben Mapletoft, DVM, MS, PhD

SPEAKERS:

Graeme McRae, Chairman, President & CEO of Bioniche Life Sciences Inc. will provide an overview of Bioniche’s existing and emerging technologies in Animal Health and Food Safety.

Dr. Gabriel Bo, DVM, MVetSc, PhD, Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina will review Bioniche’s recombinant FSH technology development program. At the completion of the two discussions, there will be a ten-minute question and answer period.

SPONSOR:


Effects of FSH on Ovarian Function and its Evolving Role in Ruminant Superovulation

CHAIRPERSON: Dr. Duncan K. Hockley (Bioniche) Dr. Matt Lucy (University of Missouri, USA) will focus on the biology and reproductive physiology of FSH and ovarian FSH receptor interactions. Dr. John Hasler (B.A. & M.A. University of Missouri, PhD University of Illinois) will review conventional as well as new evolving FSH superovulation protocols in ruminants. At the completion of these two presentations, there will be a ten minute question and answer period. SPONSOR:

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Sponsors and Patrons

Platinum Sponsor

Diamond Sponsors

Gold Sponsor

Bronze Sponsors

Grand Patrons Patrons

Contributors College of Agriculture and Bioresources (University of Saskatchewan)

Holstein Association USA, Inc.Ontario Agricultural College (University of Guelph)Ontario Veterinary College (University of Guelph)

Mary Buhr College of Agriculture (University of Saskatchewan)

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Exhibitor Floor Plan

1

2

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9

10

Company Booth NumberAgtech 3

Bioniche Life Sciences Inc 11

Canadian Veterinary Imaging Inc 4

Cedarlane Labs 16

Chemometec A/S 37

Cook Medical 43

Cryo Management Ltd 28

Cryologic 2

Current Conceptions Inc 27

Dairy Research Centre, UBC 38

ESTROTECT 1

Found Animals Foundation 41

Fujitsu Kyushu Systems 9

IMV Technologies 17

Company Booth NumberKARL STORZ Veterinary Endoscopy 14

Merial Canada Inc 33

Microptic 15

Minitub GmbH 29

Partnar Animal Health Group: EI Medical Imaging Lane Manufacturing Partnar Animal Health Syntex SA

24232122

Pfizer Animal Health 5

Prosier 10

Reproduction Resources 42

Universal Imaging 13

University of Saskatchewan, Western College of Medicine

44

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17th international congress on animal reproduction · 11:00 – 12:15 poster session 1 east exhibit...

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