1680
DESCRIPTION
1930. 1680. 1960. 1515. Optics. Focus. Confocal theory. Confocal theory. Dr. Marvin Minsky Confocal microscope: 1957. L aser. S canning. C onfocal M icroscope. LASER. L ight A mplification by the S timulated E mission of R adiation. Dichroic Mirror. Conventional / Confocal. - PowerPoint PPT PresentationTRANSCRIPT
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Dr. Marvin MinskyConfocal microscope: 1957
Confocal theory
Multiphoton exitation
800 nm
800 nm
1200 nm
1200 nm
1200 nm
400 nm
Dr. Maria Göppert-Mayer : theory of two-photon quantum transitions (two-photon absorption and emission) 1931,
Prof. Watt W. Webb et al.Two-photon laser scanning fluorescence microscopy:
1990
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Spatial photon concentration
Luo at al. Cell Structure and Function 2006, 31: 63Comparison of photoactivation of PA-GFP in vivo with single-photon (405 nm) and multiphoton (790 nm) laser light.
PhotoconversionExcitation area