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HOME ABOUT UNIQUE INNOVATIVECONNECTED TASTE NUTRITION SUSTAINABILITY

AGal-Pro®Unique enzyme solutions for poultry nutrition


Introduction

0

500

1,000

1,500

2,000

2,500

3,000

3,500

4,000

4,500

Corn Wheat SBM

En

erg

y, k

cal/

kg

Energy deficit

ME, kcal/kg

Energy

exogenous enzymes

cereal grains

greater potential to unlock

energy from SBM by using

substrate-specific enzymes


Introduction

approx. 50% of GE in SBM is not readily available

for digestion and absorption in poultry

• oligosaccharides from the raffinose series

• NSP, such as pectic polysaccharides, non-

cellulosic NSP and cellulose

lack of endogenous alpha-galactosidase production in monogastrics

specifically designed enzyme system for maximizing

the nutritional value of poultry feeds


Introduction

SOYA

MAIZE

WHEAT

Glucan sol

Glucan insol

AX sol

AX insol

Pectin sol

Pectin insol

Galactan sol

Galactan insol

0 5 10 15 20


Product Development: a-galactosidase

Free sugars released from feed by enzyme treatment

0

20

40

60

80

100

120

blank AGal-Pro

So

lub

le s

ug

ars

(m

g/g

)

Source: Broiler trial at IRTA (Spain). Broilers were fed

corn-SBM diets (SBM 40%; CP 23.4%, Lys 1.29% and

Met 0.50%)

2129

2391

1,500

1,700

1,900

2,100

2,300

2,500

Control AGal-Pro

ME

, k

cal/

kg

SB

M

Control AGal-Pro


• AGal-Pro® application into laying hens formulations

• Fundaments are the same as for broiler diets

� facilitating least cost formulations

Efficacy – LAYING HENS


University of Illinois (USA) 2010

• 896 laying hens (Hy-line W36) allocated to 128 cages (7 birds/cage) at 26

weeks of age [N=2 adjacent cages, 14 hens/N]

• Diet: corn-SBM

• 4 treatments � AME x AGal BL

• Low ME diet was formulated with -75 kcal/kg

• Egg production performance over 6 months

AME AGal

PC Recommended ME �

NC Reduced ME �

50BL Reduced ME �

100BL Reduced ME �




Diets ME (kcal/kg)

Positive Control 2,786

Negative Control 2,711

Difference (Kcal/kg) 75

SBM% 29.04

CP% 19.0


TRT

CODE

ADFI, g/hen/day

HDEP, % Total

eggs, nr/hen

Egg

weight, g/egg

Egg

mass, g/hen/d

FCR, g

feed/g egg

FCRdz, g

feed/dozen

egg

PC 101.5 89.9x 151.9a 58.3 52.52 1.9361 1.353b

NC 102.0 88.5y 148.7c 58.5 51.76 1.9735 1.383a

50BL 101.4 88.7xy 149.8bc 58.6 52.08 1.9589 1.377a

100BL 101.9 89.7xy 150.7ab 58.1 52.19 1.9558 1.362ab

LSD 1.3 1.3 1.8 0.8 1.05 0.0378 0.0227

Pv 0.713 0.095 0.007 0.655 0.544 0.275 0.039

• improved performance over a 6 month laying period

• higher hen-day egg production and total number of eggs

• no effect on egg weight or egg quality



IRTA (Spain) 2012

• 768 laying hens (Hy-line W36) allocated to 384 cages (2 birds/cage) at 22

weeks of age [N=8 adjacent cages, 16 hens/N]

• Diet: corn-SBM



• Egg production performance over 6 months

AME AGal


NC Reduced ME �

50BL Reduced ME �





Diets ME (kcal/kg)Phase 1

(22-34 wks)

Phase 2

(35-46 wks)

Positive Control 2,786 2,845

Negative Control 2,711 2,770

Difference (Kcal/kg) 75 75

SBM% 30.6 15.7

CP% 19.0 16.5


TRT CODEADFI,

g/hen/d

Laying

index, %

Egg weight,

g/egg

Egg mass,

g/hen/d

FCR, g

feed/g egg

PC 91.0b 92.0 58.3 53.6 1.697b

NC 92.0ab 91.6 57.7 52.9 1.740a

50BL 92.8a 92.0 58.1 53.5 1.736a

100BL 92.0ab 91.4 58.3 53.3 1.726a

Pooled SEM 0.52 0.52 0.22 0.39 0.0096

T1 vs (T2+T3) 0.53 0.90 0.077 0.27 0.44

T2 vs T3 0.30 0.43 0.48 0.68 0.45

Linear 0.98 0.79 0.048 0.46 0.35

• increased egg weight, at no expense of laying rate



TRT CODES (< 53

g), %

M (53-

63 g), %

L (63-73

g), %

XL (> 73 g),

%

Income*,

€/100 eggs

PC 8.50 68.6 20.2 2.73ab 8.56

NC 8.11 72.0 18.2 1.63b 8.48

50BL 7.55 70.6 19.2 2.73ab 8.57

100BL 6.42 68.4 21.9 3.28a 8.66

Pooled SEM 1.084 1.53 1.40 0.461 *L. Bellpuig (wk47)

XL €1.55/dz

L €1.20/dz

M €0.99/dz

S €0.75/dz

T1 vs (T2+T3) 0.39 0.18 0.18 0.020

T2 vs T3 0.45 0.32 0.17 0.41

Linear 0.26 0.099 0.048 0.026

• egg weight improvement is mirrored in egg classification

• improved income on egg production due to premium

prices on higher sizes



U Warmia and Mazury (Poland) 2012

• 224 laying hens (Lohnman Brown) allocated to 56 replicates (1 birds/rep) at

20 wks age

• Diet: corn-SBM



• Egg production performance over 12 weeks

• AMEn (n=16) determination, 8 weeks into the study

AME AGal


NC Reduced ME �

50BL Reduced ME �




Diets ME (kcal/kg)




SBM% 28.6

CP% 18.0



TRT CODEADFI,

g/hen/d

Laying

index, %

Egg

weight, g/egg

Egg mass, g/hen/d

FCR, g

feed/g egg

AMEn,

kcal/kg

PC 105.0 80.8ab 62.69 60.23a 1.749ab 2,463a

NC 105.8 79.7b 61.56 58.39b 1.817b 2,301c

50BL 104.8 81.1a 63.15 61.01a 1.725a 2,344bc

100BL 103.3 81.1a 63.08 60.86a 1.700a 2,452ab

SEM 0.701 0.206 0.263 0.295 0.012 21.012

Pv 0.618 0.042 0.123 0.006 0.008 0.010

• improved productivity and feed efficiency

• Increased dietary ME availability



IRTA (Spain) 2013

• 216 laying hens (Hy-line Brown) allocated to 72 cages (3 birds/cage) at 59

weeks of age [N=12]

• Diet: corn-wheat-barley-SBM

• 6 treatments

• Experimental period: 25 days

• AMEn determination by marker and total collection (22-25 d)

AME AGal

1 Control �

2 AGal-Pro BL 25 g/MT



5 Bioxylanase V 10 g/MT

6 3&5 50+10 g/MT



Diets ME (kcal/kg)

Control 2,700

SBM% 25.8

CP% 18.0



TRT CODE DM, %N retention,

%

Ether

Extract, %AME, kcal/kg AMEn, kcal/kg

Control 70.4b 35.4b 80.9b 2,912b 2,826b

25BL 72.9a 43.6a 82.7ab 2,971a 2,865a

50BL 73.2a 43.5a 82.3ab 2,973a 2,867a

100BL 72.6a 43.7a 84.9a 2,968a 2,862a

Bioxylanase 72.4a 42.3a 84.1ab 2,970a 2,867a

50BL&Bioxyl 72.6a 41.2a 84.6a 2,960a 2,860a

Root MSE 1.50 0.206 3.58 48.7 42.4

Pv 0.0001 0.0001 0.019 0.0008 0.007


• Improved nutrient retention

• Increased dietary ME availability



AMEn = 2731 (± 20.5) + 2.33 (±1.045) x dose – 0.015 (±0.0097) x dose2

Dose-response - AMEn


Kasetsart University (Thailand) 2013

• 480 laying hens (Lohnmann Brown) allocated to 160 cages (3 birds/cage) at

24 weeks of age [N=8, 5 adjacent cages, 15 hens/N]

• Diet: corn-SBM, rice bran, RSM, DDG, MBM

• 4 treatments � AME x AGal 180P


AME AGal


NC Reduced ME �

NCAG Reduced ME �

PCAG Recommended ME �



Diets ME (kcal/kg)




SBM% 15.8

CP% 17.5



TRT CODE ADFI, g/hen/day

HDEP, % Egg

weight, g/egg

Egg

mass, g/hen/d

FCR, g

feed/g egg

PC 114.6 89.1a 61.2 54.5a 2.113b

PC+AGP 180P 114.5 91.9b 61.6 56.6b 2.026a

LSD 3.15 2.14 1.08 1.84 0.064

Pv NS ** NS * *


• Increases in production and

efficiency in on-top application

• Improved conditions in the gut –

support mode of action


Kasetsart University (Thailand) 2014

• 576 laying hens (Lohnmann Brown) allocated to 24 cages (24 birds/cage) at

24 weeks of age [N=8]

• Diet: corn-SBM, rice bran oil

• 3 treatments � CRD


AME AGal


NCAG Reduced ME 220 g/MT

NC Reduced ME �



Diets ME (kcal/kg)




SBM% 24.4

CP% 17.0



Treatment ADFI(g/hen/day)

HDEP(%)

EW(g/egg)

EM(g egg/hen/d)

FCR(kg feed/kg egg)

1 PC 115.7 91.2a 61.33 55.93a 2.07b

2 NC + AG180P 117.1 90.8a 61.52 55.90a 2.10b

3 NC 116.9 89.1b 61.16 54.51b 2.15a

SEM 0.55 0.38 0.18 0.27 0.01

Pv 0.43 0.03 0.74 0.02 <0.01

b

aa


• Compensation for ME removed

in production, egg mass and FCR

• Significant reductions in feed

cost


Future developments

EU

• AGal-Pro BL approved for layers, at min doses of 100 g/MT

• Registration of Kerry’s own xylanase

• Registration of next AGal-Pro generation

EU and rest of the world

• Evaluation of AGal-Pro + XYL on CP and AA digestibility

151208 nuscience agal pro emea -...

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