13 and 15 october, 2004 chapter 13 rna splicing (and some other important stuff)

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13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

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Page 1: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

13 and 15 October, 2004

Chapter 13

RNA Splicing

(and some other important stuff)

Page 2: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Overview• Introns are removed after transcription - exons may comprise only a few

percent of the primary transcript.• The location of splicing is determined by splice site consensus sequences.• The intron is released as a lariat structure.• Splicing is carried out by a large ribonucleoprotein structure called the

spliceosome.• Spliceosome snRNPs use RNA base pairing to recognize splice sites.• Spliceosome components assemble at the 5’ splice site (U1/U6), the

Branch point (U2), and the 3’ splice site (U5).• There are at lease two types of self-splicing introns.• Splicing is highly regulated, including many instances of alternative

splicing.• Some RNAs are edited before translation.• Polyadenylation is coupled to transport.

Page 3: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Primary transcript and spliced product hybridization suggest splicing.

Page 4: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Splicing

Page 5: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Splicing Consensus Sequences

Page 6: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Unusual Electrophoretic Behavior of Intron and Dependence on snRNPs

Page 7: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

The Splicing Reaction

Page 8: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Structure of the Lariat Intermediate

Page 9: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Trans Splicing

Page 10: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

snRNP-RNA Recognition

Page 11: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Splicosome Purification

Page 12: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Splicosome Assembly

Page 13: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Base pairing between U2 and branch point sequences is demonstrated by second-site suppressor studies.

Page 14: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

4-thioU crosslinking demonstrates interactions between U6 and the 5’ splice site, U5 and the 3’ splice site.

Page 15: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

The Spliceosome Cycle

Page 16: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

The two-hybrid system demonstrates interactions between components of the commitment complex.

Page 17: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Self-Splicing Introns

Page 18: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Spliced and Self-splicing

Introns

Page 19: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Alternative Splice Sites

Page 20: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Splicing Errors

Page 21: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Regulation of Alternative

Splicing

Page 22: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Alternative Splicing

Page 23: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Alternative Splicing

Page 24: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Inhibition of Splicing

Page 25: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

AT-AC Spliceosome

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Conservation of Splicing Consenses

Page 27: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Exons encode protein

domains.

Page 28: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Gene Assembly

Page 29: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Protein Evolution

Page 30: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

RNA Editing

Page 31: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

C to U deamination

Page 32: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Editing Mechanism

Page 33: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

mRNA Transport

Page 34: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Purification of a poly-A binding protein that stimulates poly-A polymerase (PABII)

Page 35: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Model for polyadenylation

Page 36: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)
Page 37: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

Title

Page 38: 13 and 15 October, 2004 Chapter 13 RNA Splicing (and some other important stuff)

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