Figure S1. Phylogenetic tree of TaLTPIb.1, TaLTPIb.5 and TaLTPId.1. Putative 9 KDa nsLTP genes in rice and wheat were downloaded

from NCBI and employed for pairwise alignment by clustalW. The eight cysteine motif was used for phylogenetic tree construction by

MEGA 5.0 using Maximum likelihood algorithm with 1,000 bootstrap replicates.

Figure S2 RT-PCR conditions of TaLTPIb.1, TaLTPIb.5 and TaLTPId.1. (A) Phenotypes of wheat seedlings under MeJA (100 μM), SA

(2 mM), ABA (100 μM), IAA (100 μM) treatments; (B) Semi-quantitative RT-PCR of TaLTPs under control conditions in hormone

treatments (C) Real-time PCR of TaLTPs under control conditions in hormone treatments; (D) Semi-quantitative RT-PCR of TaLTPs under

control conditions in abiotic stress treatments; (E) Real-time PCR of TaLTPs under control conditions in abiotic stress treatments.

0h

6h

12h

24h

Control MeJA SA ABA IAA

0h 20min 40min 1h 3h 9h 24h 48h 72h TaLTPb.1

TaLTPb.5

TaLTPd.1

Ta18srRNA

0h 6h 12h 24h

TaLTPb.1

TaLTPb.5

TaLTPd.1

Ta18srRNA

TaLTPb.1 TaLTPb.5 TaLTPd.10

0.20.40.60.8

11.21.41.6 6h

12h24h

TaLTPb.1 TaLTPb.5 TaLTPd.10

0.20.40.60.8

11.21.4

20min40min1h3h9h24h48h

A B

C

D

E

Figure S3 Subcellular localization of TaLTP:EGFP fusion proteins in onion epidermal cells. Onion epidermal cells transiently

transformed with either the TaLTIb.1:EGFP (A) or EGFP control (B) were incubated in 0.8 M mannitol to induce plasmolysis and then

imaged by confocal microscopy. pm, plasma membrane; cw, cell wall; nu, nuclear

EGFP Bright Field Merge

TaL

TP

Ib.1

:EG

FP

cw

pm

35S

:EG

FP

nu

A1A2 A3

B1B2 B3