12/13/2015 1 genes and how they work computer filename: genes1.ppt developed by hugh b. fackrell
TRANSCRIPT
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Genes and How They Work
Computer Filename: genes1.ppt
Developed by Hugh B. Fackrell
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Overview
Genes are composed of DNA within the chromosome
Genes code for proteins DNA is transcripted into RNA RNA is translated into protein by
ribosomes Genes regulated by control of RNA
production
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Lecture Outline
Genetic Code Gene Expression transcription translation Protein Synthesis Gene Regulation Gene Architecture
Genes and How They WorkGenes and How They Work
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Concepts from OAC
Structure & Function of DNA & RNA Genetic code Transcription & translation Protein synthesis Gene Regulation: operators, promotors ,
repressors
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Background Terms & Concepts
Structure of DNA & RNA Ribosomes Enzymes Structure of Eukaryotic chromosomes
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The Genetic Code
Genetic information is stored in DNA sequences found in– Chromosomes– Mitochondria: 16 KB, 13 genes– Chloroplasts: 120-160 KB, 120 genes
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Structure of the Genetic Code
DNA : sequences of four nucleotides A,T,G,C CODONS : blueprint for the polypeptide Start & Stop Signals: Where to read the blueprint Regulatory Sequences: When to read the
blueprint– promotors– operators– enhancers
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CODONS
sequences of three adjacent nucleotides that code for one amino acid or polypeptide termination
64 codons encrypt of ~ 20 amino acids degenerate RNA: ACU, ACC,ACA, ACG
threonine degenerate DNA: TGA, TGG,TGT, TGC
threonine
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Start Codon
RNA: AUG
DNA: TAC also codes for methionine
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Stop Codon
Nonsense Codons RNA: UAA,UAG,UGA DNA: ATT, ATC, ACT
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Alternate Genetic Codes
Sequence Chromosome Mitochondrion
TGA Stop Trypt
ATA Isoleucine Methionine
AGA Arginine Stop
AGG Arginine Stop
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Interpreting the Genetic Code
Two step process :
– Transcription DNA --> RNA
– Translation RNA--> protein
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RNA
Ribosomal RNA Transfer RNA Messenger RNA
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Ribosomal RNA
called rRNA found in Ribosomes probably catalytic site provides site for protein assembly ribosomal structure different in
eukaryotes and prokaryotes
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Prokaryotic Ribosomal RNA
large subunit 5s(120), 23s (2900) Small subunit 16s (1540)
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Eukaryotic Ribosomal RNA
Large subunit 5s(120) 28s (4700) 5.8s (160) Small subunit 18s(1900)
18 S18 S 28 S28 S 5.8 S5.8 S
Transcribed Spacer RNATranscribed Spacer RNA
Ribosomal RNARibosomal RNA
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Ribosomal RNA
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Transfer RNA
called tRNA Smaller than mRNA or rRNA found in cytoplasm 40 different kinds of tRNA transports amino acids to ribosome positions amino acids on elongating
polypeptide
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tRNA
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Messenger RNA
called mRNA long single strand of RNA copy of the DNA to be decoded transfers information from the nucleus to
the cytoplasm for decoding
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Transcription
occurs in nucleus of eukaryotes
– cytoplasm of prokaryotes DNA unravels- single strand RNA Polymerase binds to Promotor site Reads from start sequence(TAC) assembles complementary single strand of RNA (primary
RNA) Disengages when reaches stop codon
Production of an RNA copy of the geneProduction of an RNA copy of the gene
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RNA being read from DNA
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Prokaryotic Transcription and Translation
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Antibody Structure
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Eukaryotic Transcription & Translation
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Intron Splicing
Regulated at DNA level by restriction endonucleases
Regulated at RNA level by SPLICEOSOMES
GU....AG sequences thalassemia syndromes
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Splicing of DNA
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Gene Rearrangement & Processing
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mRNA Splicing
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DNA Loops