Environmental Consultants And Laboratory Services

Westport Public Schools Attn. Craig Schmarr 110 Myrtle Ave Westport, CT

Re: Post Remediation Inspection Coleytown Middle School

Dear Mr. Schmarr.

(203) 324-2222

11 HYGENIX, INC.

Fax (203) 324-3876

49 Woodside Street Stamford, CT 06902

September 29, 2018

This letter summarizes the post remediation inspection and sampling performed at the above referenced school on September 27, 2018.At the time of the inspection, Rooms 113,203, 208 appeared to be clean and dry.

To quantify for airborne mold, indoor air samples and one outdoor reference air sample were collected using ZefonAir-0-Cell cassettes in accordance with the manufacturer's recommended protocol. All samples were delivered to Pure Earth Environmental Laboratory Inc., where they were analyzed for mold by dlrect microscopic examination. The following table is a summary of the attached lab results:

TABLE 1. AIR-0-CELL SAMPLE RESULTS

Location Total Counts Predominant Species Individual Counts -·--··--------- .. --�pr/m�--. ·-____ .. Spr/m3 ·-- _

Outdoors -·-·-··-···

Room 203

Room 208

j Room 113

6347

533

960

587

Ascospores 4373 Basidiospo�es 1173

As co spores 160

Cladosporiu_ m 267 -+ Ascospores 587

. Basid�ospores 267

Ascospores 213

I Cladosporium 160

The following typical indoor mold spore concentration ranges were suggested as a "guide to evaluating the relative degree of indoor airborne mold spore amplification'' by Daniel Baxter, the inventor of the Air-0-Cell mold spore trap, the most widely used b;oaerosol sampling device:

TABLE 2 .

... Description-··-·-.. -·-.. ---·--.... Spores( CTS/M3) Predominan! Types

"Clean" building .. less tha_n 2,000 . Total for all spores -----.. ·----less than 700 Penicillium,A,_�f!rgi/Jus

Possible Indoor Amplifi_�a�_ion 1,000-5,000 Penicillium, Aspergillus, Cladosporium Indoor Amplification likely present 5 ,000-10 ,000

... ...

�h ronic Indoor Amplification 10,000-500,000 Penicillium, Aspergi/Jus, Cladosporium_ nadequate flood cleanup or active 50 ,000-10 ,000 ,000 Penicillium, Aspergil/us, Stachybotrys,

lindoor demolition of contaminated Cladosporium, Chaetomi/Jm, Basidiomycete surfaces Trichoderma, Ulocladium, etc.

s,

Westport Public Schools

Coleytown Middle School

September 2018

• Room 203, 208, 11�-- -Airborne Mold Test- Satisfactory -The sample results were not elevated and have

satisfied the criteria for "Clean" building.• Room 203,208,113 -Visual Inspection -Satisfactory-All areas/surfaces appeared to be clean and dry.

Work area containment have met post remediation standards.

Westport Public Schools

Coleytown Middle School

AIR TEST LAB RESULTS

July 2018

�- Arnobioloqy L1'boRAroRy � ASSOCIATES, INCOf{PORi\TfD

Expertise Since 1997

Certificate of Analysis

AIHA-LAP EMLAP# 102747

7184 North Park Drive

Pennsauken, New Jersey 08109 (856)486-1177

Hygenix, Inc 49 Woodside St. Stamford , Connecticut 06902 Project: HYGENIX WPS/ CMS 0927

Condition of Sample( s) Upon Receipt: Acceptable

1054 Spore Trap Analysis: SOP 3 8 Client Sample Number 26606089

Sample Location Sample Volume (L) Lab Sample Number Spore Identification

ascospores basidiospores Cladosporium

hyphal elements Smuts,Periconia,Myxomycetes

Raw Ctl ...

3 I z I s I - I- l

203

75

18036411-001

spr/m3 I % Ttl I In/Out Raw Ctl 1 eo I 30 l 1121 a2 l 101 I 20 I 1111 22 I 261 I so I 112 12 I

I - I - 1 I I - I - 2 I

Debris Rating 3

www.aerobiology.net

Date Collected: 09/27/2018 Date Received: 09/28/2018 Date Analyzed: 09/28/2018 Date Reported: 09/28/2018

Project ID: 18036411 Page 1 of 3

26606121

OUTDOOR

75

18036411-002

spr/m3 I % Ttl I In/Out

4373 I ee I -1113 I 1a I -640 I 10 I -s3 I 1 I -

107 I 2 I -Debris Rating 3

Analytical Sensitivity Analytical Sensitivity: 13 spr/m3 Analytical Sensitivity: 13 spr/m3

Comments L�-�al *See Footnotes 10 l 533 l-100%J 1112 119 I 634_�_J-100%I -

.. ·--·

Client Sample Number 26605851 26606121

Sample Location 208 OUTDOOR

Sample Volume {L) 75 75

Lab Sample Number 18036411-003 18036411-002

Spore Identification Raw Ct[ spr/m3 I % Ttl Un/Out Raw CtJ spr/m3 I %Ttl In/Out ascospores 11 I 587 l 61 I 1/7 82 l 4373 I 69 -

basidiospores 5 I 267 ! 28 I 1/4 22 I 1173 I 18 I -

Cladosporium 2 I 107 I 11 I 1/6 12 I 640 I 10 I -hyphal elements - I - I - I - 1 I 53 I 1 I -

Smuts,Periconia,Myxomycetes - l - I - I ·- 2 I 107 I 2 I �

Debris Rating 3 Debris Rating 3

Analytical Sensitivity Analytical Sensitivity: 13 spr/m3 Analytical Sensitivity: 13 spr/m3

Comments Total *See Footnotes 18 I 960 1-100%1 1/7 119 I 6347 1-100% -

'-- AERobioloqy L,boRAIORY � A.%00.\TES, INCORPORATED

Expertise Since 1997

Hygenix, Inc 49 Woodside St. Stamford , Connecticut 06902 Project: HYGENIX WPS/ CMS 0927

Certificate of Analysis

AIHA-LAP EMLAP# 102747

Condition of Sample(s) Upon Receipt: Acceptable

Client Sample Number 26607363

Sample Location 113

Sample Volume (L) 75

Lab Sample Number 18036411..004

Spore Identification Raw Ctl spr/m3 I %TII ! In/Outascospores 4 ,-- 213 I 36 I 1121

basidiospores - I - - I -

Cladosporium 3 I 160 I 27 I 1/4 hyphal elements - I - I - I -

Penicillium/Aspergillus group 3 I 160 27 I -Smuts,Periconia,Myxomycetes 1 I 53 9 I 1/2

Debris Rating 3

Analytical Sensitivity Analytical Sensitivity: 13 spr/m3

Comments

Total *See Footnotes 11 I 587 1�100%1. 1/11

7184 North Park Drive Pennsauken, New Jersey 08109

(856) 486-1177www.aerobiology.net

Date Collected: 09/27/2018 Date Received: 09/28/2018 Date Analyzed: 09/28/2018 Date Reported: 09/28/2018

Project ID: 18036411 Page 2 of 3

26606121

OUTDOOR

75

18036411-002

Raw Ct! spr/m3 I % Ttl I In/Out

82 I 4373 I 69 I -

22 I 1173 I 18 I -

12 I 640 I 10 I -1 I 53 I 1 I -

- I - I - I -2 I 107 I 2 I -

Debris Rating 3

Analytical Sensitivity: 13 spr/m3

119 I 6347 1-100%1 -

�. Anmbioloqy lAbor<ATORY ·� ASSOCIATES, INCORPORATED

Expertise Since 7997

Hygenix, Inc

Certificate of Analysis AIHA-LAP EMLAP# 1 02747

71 84 North Park Drive Pennsauken, New Jersey 081 09

(856) 486-1 1 77 www.aerobiology.net

Date Collected: Date Received:

49 Woodside St. Date Analyzed:

09/27/201 8 09/28/201 8 09/28/201 8 09/28/201 8

I

Stamford , Connecticut 06902 Project: HYGENIX WPS/ CMS 0927 Condition of Sample(s) Upon Receipt: Acceptable

Date Reported: Project ID:

Footnotes and Additional Report Information

Debris Rating Table -

1 Minimal (<5%) particulate present Reported values are minimally affected by particulate load.

1 803641 1 Page 3 of 3

2 5% to 25% of the trace occluded with Negative bias is expected. The degree of bias increases directly with the percent particulate of the trace that is occluded.

3 26% to 75% of the trace occluded with Negative bias is expected. The degree of bias increases directly with the percent particulate of the trace that is occluded.

4 75% to 90% of the trace occluded with Negative bias is expected. The degree of bias increases directly with the percent particulate of the trace that is occluded.

Greater than 90% of the trace occluded with Quantification not possible due to large negative bias. A new sample should be 5 collected at a shorter time interval or other measures taken to reduce particulate particulate

load. ···--·

1 . Penicillium/Aspergillus group spores are characterized by their small size, round to ovoid shape, being unicellular, and usually colorless to lightly pigmented. There are numerous genera of fungi whose spore morphology is similar to that of the Penicillium/Aspergillus type. Two common examples would be Paecilomyces and Acremonium. Although the majority of spores placed in this group are Penicillium, Aspergillus, or a combination of both. Keep in mind that these are not the only two possibilities. 2. Ascospores are sexually produced fungal spores formed within an ascus. An ascus is a sac-like structure designed to discharge the ascospores into theenvironment, e.g. Ascobolus.3. Basidiospores are typically blown indoors from outdoors and rarely have an indoor source. However, in certain situations a high basidiospore count indoorsmay be indicative of a wood decay problem or wet soil.4. The colorless group contains colorless spores which were unidentifiable to a specific genus. Examples of this group include Acremonium, Aphanocladium,Beauveria, Chrysosporium, Engyodontium microconidia, yeast, some arthrospores, as well as many others. 5. Hyphae are the vegetative mode of fungi. Hyphal elements are fragments of individual Hyphae. They can break apart and become airborne much like spores and are potentially allergenic. A mass of hyphal elements is termed the mycelium. Hyphae in high concentration may be indicative of colonization.6. Dash (-) in this report, under raw count column means 'not detected (ND)'; otherwise 'not applicable' (NA).7. The positive-hole correction factor is a statistical tool which calculates a probable count from the raw count, taking into consideration that multiple particles can impact on the same hole; for this reason the sum of the calculated counts may be less than the positive hate corrected total. 8. Due to rounding totals may not equal 1 00%.9. Analytical Sensitivity for each spores is different for Non-viable sample when the spores are read at different percentage. Analytical Sensitivity is calculated as spr/m3 divided by raw count. spr/m3 = raw counts x (100/ % read) x (1 000/Sample volume). If Analytical Sensitivity is 1 3 spr/m3 at 1 00% read, Analytical Sensitivity at 50% read would be 27 spr/m3 , which is 2 times higher. Analytical Sensitivity provided on the report is based on an assumed 1 00% of the trace being analyzed. 1 0. Minimum Reporting Limits (MRL) for BULKS, DUSTS, SWABS, and WATER samples are a calculation based on the sample size and the dilution plate on which the organism was counted. Results are a compilation of counts taken from multiple dilutions and multiple medias. This means that every genus of fungi or bacteria recovered can be counted on the plate on which it is best represented. 1 1 . If the final quantitative result is corrected for contamination based on the blanK, the blank correction is stated f n the sample comments section of the report. 12. The results in this report are related to this project and these samples only.13. For samples vvith an air volume of < 1 OOL, the number of significant figures in the result should be considered (2) two. For samples with air volumesbetween 100-999l, the number of significant figures in the result should considered (3) three. For example, a sample with a result of 55,443 spr/m3 from a 75Lsample using significant figures should be considered 55,000. The same result of 55,443 from a 150L sample using significant figures should be considered55,400 spr/m3. 14. If the In/Out ratio is greater than 1 00 times it is indicated >100/1 , rather than showing the real value.Terminology Used in Direct Exam Reporting

Conidiophores are a type of modified hyphae from which spores are born. When seen on a surface sample in moderate to numerousconcentrations they may be indicative of fungal growth.

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Suzanne S. Blevins, B.S . , SM (ASCP) Laboratory Director

© 2018 Aerobiology Laboratory Associates1 In�. All rightli re�erved.

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