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EVEREST BIOTECH PVT. LTD.Khumbftar, Lalitpur

P.O.BOX:21608

,*,' rrr-r-fi?Ifli?*%?Fax:977-1-5il0141

7h April,2014

CERTIFICATE OF EXPERIENCE

This is to certify that Mr. Ajay Kumar Yadav joined this company on 9th July,20l2as a full timeProcessing Lab Assistant (Research Assistant) till l6d, April, 2013.During his tenure, we found him to be a diligent, professional, co-operative, and optimistic resultoriented person with strong theoretical and practical understanding of work ..qri."."nts. He hassuccessfully completed many job related in-house training program offered by the organization.With his outgoing personality, good sense of humor and u'Uifity to work well as an individual oras a team member, Mr. Yadav has helped to develop synergistic working .rriron-"rt at thisorganization.

ibirGet instruction and p.lan antibody purification jobs designed to ensure products qualityconsistent with established standards. J - - ----o^^-Y

2' Analyze the result obtained from ELISA experiment and product specification from serumprocessing department.3' Undertake the experimental work including: Serum processing, peptide conjugation,

ammonium -sulphate precipitation of proteins and their .rr".oriuiog.uprri. affinitypurification, handling and preparation of tissue homogenate from uumanluouse and Rat

tissues for detection oftarget proteins.4' Conduct analytical studies of purified proteins: Perform gene expression analysis usingbioinformatics tools, separation of proteins using SDS-Facp and antibody detection

using immunoassay technique, specifically westeri Blotting and ELISA.5' Participate in the co-supervision of undergraduate students driing theirproject workoverall, Mr' Yadav has performed his duties and responsibilities cheerfully with due attention toevery detail, all times. I feel no doubt to say thq his intelligent research skills, learning aptitudewith fervid enthusiasm to shoulderany responsibilities and"professional endeavors will pave outinnovative avenues to any organizations. on behalf_of the oiganization, I take this opportunity towish Mr' Yadav all the very best in his future endeavors. il.ur" feef free to contact us if youhave any queries regarding his employment.

Bikash Pandey(General Manager)Everest Biotech Pvt. LtdKathmanduiNepalpandey @everestbiotech. comwww.everestbiotech. com

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