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DESCRIPTION
1 2 3 4 5 6. 1 2 3 4 5 6 7 8. 10000 8000 6000 5000 4000 3500 3000 2500 2000 1500 1200 1031 900 800 700 600 500. CHS tom. PCU416 . Lane1-6: clones 1-6 uncut; Lane7 : PCU416 uncut; Lane8 : marker. - PowerPoint PPT PresentationTRANSCRIPT
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CHS tom. PCU416.
Lane1-6: clones 1-6 uncut; Lane7: PCU416 uncut; Lane8: marker
CHS TOM. PCU416 CLONE1 .
Lane1: clone1 uncut; Lane2: clone1 cut with EcoRI; Lane3: clone1 cut with EcoRI, XhoI; Lane4: PCU416 uncut; Lane5: PCU416 cut EcoRI, Xho1; Lane6: marker
1 2 3 4 5 6 7 81 2 3 4 5 6
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4CL tom. PMY5. Lane1-6: clones 1-6 uncut; Lane7: PMY5 uncut; Lane8: marker
4CL TOM. PMY5 CLONE 6 . Lane1: clone6 uncut; Lane2: clone6 cut with PstI; Lane3: clone6 cut with Pst, XhoI; Lane4: PMY5 uncut; Lane5: PMY5 cut Pst, Xho1; Lane6: marker
1 2 3 4 5 6 7 81 2 3 4 5 6
1 2 3 4 5 6 7 1 2 3 4 5 6
1 2 3 4 5 6 7
CHI pet. in pMY4
Lanes 1- 4: clones 1,2,5,6 of CHI in pmy4 uncut;
Lane5: marker; Lane6: pmy4 uncut
Lanes 1-2: clone1,clone6 CHI in pmy4 cut with Not1
and Sal1; Lane3: pmy4 cut with Not1 and Sal1; Lane4:
marker; Lanes 5-6: clones 1,6 CHI in pmy4 uncut;
Lane7: pmy4 uncut
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Restricted pMY4 Not1, Sal1
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Cloning of 4CL into pRS305 and CUP1_CYC1 cassette into pRS306
1 2 3 4 5 6 7
Lanes 1-3: clones 1,2,3 of CUP1_CYC1
cassette into pRS306; Lanes 4-6: clones
1,2,3 of 4CL into pRS305 ; Lane7: marker
Lane1: CUP1_CYC1 cassette into pRS306 cut with Sac1;
Lane2: PCU416 cut with Sac1; Lane3: 4CL in prs305 cut
with Sac1; 4CL pmy5 cut with Sac1; Lanes 5-7: PRS306 +
cassette,pcu416,4CL PRS305 uncut; Lane 8: MarkerChecking of the ligation by plasmids size:
PCU416 5300 bp
PRS306+CASSETTE 5031bp
4CL PMY5 8213bp
4CL PRS305(+CASSETTE) 7707bp
1 2 3 4 5 6 7 8
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PRS305 cut with Sac1,Apa1; PRS306 cut with Sac2, Kpn1
1 2 3 4 5 6 7
Lanes 1-3: PRS305 cut with Sac1,Apa1;
Lane 4: marker; Lanes 5-7: PRS306 cut
with Sac2, Kpn1
Lane1: CHI_prs304_sac1_kpn; Lane 2: marker
Lane 1: PRS303_CUP1 SacI, ApaI; Lane 2: PRS313_CUP1
SacI, ApaI ; Lane 3: MCS _PFA6 IN PMY7 NotI, ClaI; Lane 4:
marker
1 2 1 2 3 4 5 6 7 8 9 10
1 2 3 4
Lanes 1-3: PRS303_CUP1 clones 1,2,3; Lanes 4-6:
PRS313_CUP1 clones 1,2,3; Lanes 7-9: MCS
pFA6_PMY7 clones 1,2,3; Lane 10: marker
Lane1: PRS306 +CASSETTE Sac1, Kpn1; Lane2:
PCU416 Sac1, Kpn1 ; Lane3: 4CL PRS305 Sac1,
Apa1; Lane4: 4CL PMY5 Sac1, Apa1; Lane5:
PRS306 uncut ;Lane6: PCU416 uncut;
Lane7:4CLPRS305uncut; Lane8: MARKER
1 2 3 4 5 6 7 8
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1 2 3 4 5
Lanes 1-2: CHI PRS304 clones 5,6,7; Lanes 4-6:
CHS PRS306 clones 5,6,7; Lane 7: PMY4;
Lane8: PCU416
Lane1: CHI PRS304 Not1, Sal1; Lane 2: CHI PMY4 Not1, Sal1; Lane3: CHS PRS306 EcoRI, Xho; Lane4: CHS PCU416 EcoRI, Xho; Lane 5: marker