10. bibliographyshodhganga.inflibnet.ac.in/bitstream/10603/2334/15/15... · 2015. 12. 4. ·...

Experimental Design and Observations

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APPENDIX – I

Composition of media

(A) SOLID MEDIA

Sr. No. Name of Solid Media Components Quantity (g/l)1) Yeast Extract Agar Yeast Extract

Malt Extract

Dextrose

Agar

DW

4.0

10.0

4.0

5.0

1 l2) Wheat Grain Agar Crushed wheat grain

Agar

DW

40.0

20.0

l l3) Coriander Agar Coriander seeds

Agar

DW

10.0

20.0

1 l4) Potato Dextrose Agar Peeled potatoes

Dextrose

Agar

DW

200.0

20.0

20.0

1 l5) Malt Extract Agar Malt extract

Agar

DW

25.0

20.0

1 l

6) Wood Extract Agar Wood chips

Dextrose

250.0

20.0

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Agar

DW

10.0

1 l7) Yeast Glucose Agar Yeast extract

Glucose

Agar

DW

4.0

20.0

15.0

1 l8) Carrot Agar Grated carrot

Agar

DW

20.0

20.0

1 l9) Czapek Dox Agar NaNO3

K2HPO4

MgSO4. 7H2O

KCl

FeSO4 . 7H2O

Sucrose

Agar

DW

2.0

1.0

0.5

0.5

0.01

30.0

15.0

1 l10

)

Maize Extract Agar Crushed maize grains

Agar

DW

40.0

20.0

1 l11

)

Dimmick Medium Glucose

K2HPO4

NaCl

MgSO4.7H2O

NaNO3

Agar

DW

10.0

0.5

0.5

0.2

2.0

15.0

1 l12 Pea Extract Agar Crushed pea seeds 40.0

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Agar

DW

20.0

1 l13

)

Yeast Potato Dextrose Peeled potatoes

Yeast extract

Dextrose

Agar

DW

250.0

1.0

20.0

20.0

1 l14

)

Potato Carrot Agar Potato

Carrot

Agar

DW

20.0

20.0

20.0

1 l15

)

Gram Extract Agar Gram

Agar

DW

40.0

20.0

1 l

(B) LIQUID MEDIA

Sr. No. Name of Liquid Media Components Quantity (g/l)

1) Richard's Solution KNO3

K2HPO4

MgSO4.7H2O

FeCl3

10.0

5.0

2.5

0.02

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Sr. No. Name of Liquid Media Components Quantity (g/l)

Sucrose

DW

50.0

1l2) Glucose Asparagine Medium Glucose

Asparagine

MgSO4.7H2O

KH2PO4

DW

30.0

1.0

0.5

1.5

1 l3) Maltose Peptone Broth Maltose

Peptone

DW

3.0

1.0

1 l4) Glucose Peptone Medium Glucose

Peptone

DW

5.0

20.0

1 l5) Peptone water Peptone

NaCl

DW

10.0

5.0

1 l

6) Asthana & Hawker Medium KNO3

KH2PO4

MgSO4.7H2O

Glucose

DW

3.5

1.75

0.75

5.0

1 l7) Koser Citrate Medium NaCl

MgSO4.7H2O

5.0

0.2

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NH4H2PO4

K2HPO4

Citric Acid

DW

1.0

1.0

2.0

1 l8) Dimmick Medium K2HPO4

NaCl

MgSO4.7H2O

NaNO3

Glucose

DW

0.5

0.5

0.2

2.0

10.0

1 l9) Czapek Dox Medium NaNO3

K2HPO4

KCl

MgSO4.7H2O

FeSO4

Sucrose

DW

3.0

1.0

0.5

0.5

0.01

30.0

1 l10) Yeast Glucose Medium Yeast extract

Glucose

K2HPO4

MgSO4.7H2O

DW

4.0

20.0

1.0

0.5

1 l11) Potato Dextrose Broth Peeled potatoes

Dextrose

NaCl

DW

500.0

10.0

1.0

1 l12) Pea Broth Dried Peas 150.0

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DW 1 l13) Rye Seed Medium Rye Seed

Dextrose

DW

200.0

10.0

1 l14) Malt Broth Malt Extract

Dextrose

Peptone

KH2PO4

MgSO4

DW

5.0

15.0

1.0

1.0

0.5

1 l15) Will Mineral Salts Medium KCl

KH2PO4

MgSO4.7H2O

K2CO3

DW

0.05

0.10

0.05

0.30

1 lAPPENDIX – II

Citrate Buffer

1) 0.1 M solution of Citric acid 21.06 gm Citric acid in 1000 ml of distilled water

2) 0.2 M solution of Sodium citrate 29.41 gm Sodium citrate in 1000 ml of distilled water

Prepare 100 ml by adding double distilled water

Sr. No.

pH Citric acid (ml) Sodium citrate (ml)

1 3.0 46.5 3.5

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2 3.5 40.0 10.03 4.0 33.0 17.04 4.5 28.0 22.05 5.0 20.5 29.56 5.5 16.0 34.07 6.0 9.5 41.5

APPENDIX – III

Phosphate Buffer

1) 0.2 M solution of Monobasic Sodium phosphate

27.8 gm in 1000 ml of distilled water

2) 0.2 M solution of Dibasic Sodium phosphate

53.65 gm in 1000 ml of distilled water

2) Na2HPO4.7H2O or Na2HPO4.12H2O in 1000 ml

Prepare 100 ml by adding double distilled water

Sr.

No.

pH Na2H2PO4 (ml) Na2HPO4 (ml)

1 6.5 34.25 15.752 7.0 19.75 30.53 7.5 8.0 42.04 8.0 2.65 47.355 8.5 48.25 1.75

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6 9.0 44.25 5.757 9.5 39.5 10.58 10.0 31.5 18.5

APPENDIX – IV

Glycine and NaOH Buffer

1) 0.1 M solution of Glycine 7.5 gm of Glycine in 1000 ml of distilled water

2) 0.2 M solution of NaOH 4.0 gm NaOH in 1000 ml of distilled water

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APPENDIX - V

LIST OF PUBLICATIONS AND CONFERENCES ATTENDED

PAPERS PUBLISHED

♦ Atri N.S., Upadhyay R.C., Singh Rajinder and Saini M.K. 2007 Effect of culture

media and physical factors on vegetative growth of Lentinus squarrosulus (Mont.)

Singer. Journal Punjab Academy of Sciences 4 (1&2 ) : 91 – 94.

♦ Atri N.S., Upadhyay R.C., Singh Rajinder and Saini M.K. 2009. Effect of carbon

and nitrogen sources on the vegetative growth of Lentinus squarrosulus (Mont.) Singer.

Indian Journal of Mushrooms 27 (1&2):29 – 32.

CONFERENCES ATTENDED

♦ National Symposium on "Researches in Fungal Biology - Emerging Trends" organized by

the Department of Botany, Punjabi University Patiala on January

30 – 31 , 2007.

♦ International Conference on "Mushroom Biology and Biotechnology" at Directorate of

Mushroom Research, Chambaghat, Solan on February 10-11, 2007.

♦ National Conference on “Botanical Researches – Present scenario” organized by

Department of Botany, Punjabi University, Patiala on February 18-19, 2010.

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