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New Zealand, November 2005

Mark R. Bartlett, Ph.D., Senior Director, Pharmanex Research & Development

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3

Green Tea and Longevity

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68

70

72

74

76

78

80

82

Mea

n A

ge

of

Dea

th

.

Men Women

3 or less

4 to 9

over 10

Green TeaConsumption(Cups/Day)

Prospective, 11-year cohort study of 8,552 subjects in Yoshimi, Japan, 1986-1997. From: H. Fujiki et al., Ann. N.Y.Acad.Sci., 928:274, 2001; used with author’s permission

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　　 HOW LONG WILL YOU LIVE?HOW LONG WILL YOU LIVE?

“…　there　is　a　correlation　of　the　concentration　of　a　number　of　different　antioxidants　in　serum　with　increasing　lifespan　of　mammalian　species.”

Dr. Richard Cutler, National Institute of ageing,

Washington, D.C. Dr. Richard Cutler

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Highly reactive, short-lived molecules

Damage cells and genetic material (DNA) 70,000 free radical attacks

on DNA every day in a cell Ageing process Implicated in most health

conditions Many different types of free

radicals

Free Radicals

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Most Vulnerable to free radicals…

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Where do Free Radicals come from?• Environmental pollution (smog, ozone, NO2) • Radiation exposure:

– sunlight (UV-light), X-rays, etc.• Smoking:

– 10,000,000,000,000,000 free radicals/cigarette!

• Eating and Breathing: metabolism in your mitochondria

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Antioxidants fight free radicals Slow down ageing processes & improve health:

over 30,000 research studies on antioxidant health benefits

affecting over 50 health conditions, including cardiovascular function, eye health, CNS health, skin health, etc.

Many antioxidants work together: “Antioxidant defence System”

Relies on a steady supply of dietary antioxidants

Stop Free Radicals with Antioxidants

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“… low levels of the antioxidant vitamins (vitamins A, E, and C) may increase risk for several chronic diseases. Most people do not consume an optimal amount of all vitamins by diet alone.”

“It appears prudent for all adults to take vitamin supplements.”

Journal of the American Medical Association (JAMA) June 2002

Robert H. Fletcher, MD, MSc; Kathleen M. Fairfield, MD, DrPH, Harvard UniversityJournal of the American Medical Association 2002; 287: 3127-3129

A Landmark Endorsement

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Antioxidants: More than just One…• Vitamin E: 8 natural forms• Vitamin C • Metallo-enzymes (cofactors)

– Selenium, Zinc, Manganese• Flavonoids:

– Catechins, polyphenols etc

• Carotenoids:– Β-carotene, lutein, lycopene, α-carotene

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Antioxidant protection

Vitamin CLutein

Lycopene

Betacarotene

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LifePak® 40+ Antioxidants

• All 8 forms of natural vitamin E• Buffered vitamin C• Selenium, Copper, Zinc, Manganese• 6 Carotenoids:

– as found in 5-10 fruits & vegetables per day • Over 20 Flavonoids:

– 6 Catechins, Quercetin, naringenin, hesperidin, 3 Soy isoflavones, Over 10 grape seed polyphenols

• Alpha-Lipoic Acid: 25 mg

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LifePak is NOT just a multivitamin!

What is LifePak®

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You Choose

Spend all day eating this

Or add LifePak to

your lifestyle

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LifePak® Science is Measurable

• First to show antioxidant and cardiovascular benefits in double-blind studies

• 200,000+ published studies on LifePak’s antioxidants

• Nearly 800,000 published studies on LifePak’s 50+ ingredients

• First multivitamin/mineral supplement to pass top 3 safety certification tests

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LifePak® Clinical Study

42.1 42

43.4

50.9

30

35

40

45

50

55

60

65

Baseline Endpoint

PlaceboLifePak

p < 0.001

331299

335

716

0

200

400

600

800

1000

1200

Baseline Endpoint

PlaceboLifePak

p < 0.001

3.4773.429

6.248

3.305

0

1

2

3

4

5

6

7

8

Baseline Endpoint

PlaceboLifePak

p < 0.001

Smidt CR, Seidehamel RJ, Devaraj S, Jialal I. FASEB Journal Vol.13 No.4, p.A546, March 1999.

LDL Protection*

Vitamin E

Beta-carotene

Antioxidant and Cardiovascular Health

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The QuestionsSkin Carotenoids – the Most Reliable Predictor of Antioxidant Status

Carotenoids Vitamins C & E Body’s InternalAntioxidant Systems

• Carotenoids are the body’s first line of defence against free radicals.• Carotenoid levels are most sensitive to oxidative stress

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VITAMIN CcycleVITAMIN E

cycle

THIOLcycle

NAD(P)H + H+

NAD(P)+

Lipid–water interface

ROOHROH

PUFA

O2– and other radicals

Metabolism, strenuous exercise,cigarette smoke, pollution, eating

ROO•RO•

The Antioxidant Network

Carotenoids

Carotenoids

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Antioxidant Status can be measured …

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Pharmanex has the two most powerful tools of the entire nutritional supplement industry worldwide:

LifePak: one of the best multi-nutrient supplements

BioPhotonic Scanner: the best consumer test for antioxidant nutrition.

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See YOUR antioxidant score in 3 minutes!

• Measures your own antioxidant protection (skin carotenoids).

• Measure antioxidant effect of LifePak in your own body!

• Easy & non-invasive.• Scientifically and clinically

validated.

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• Invented by Werner Gellermann, Ph.D. (U. Utah)• Patent rights licensed to Pharmanex• Scientifically validated (4 clinical studies)• Measures carotenoid antioxidants in skin

– Important part of the body’s antioxidant defence system

– good indicator of your antioxidant status• Highly specific for carotenoids • Based on Raman spectroscopy

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What is Raman Spectroscopy?Intense monochromatic light (one colour only) interacts with molecules that have vibrational energy (e.g., carotenoids) and gets scattered at a higher wavelength (colour). Sir C. V. Raman, Nobel Prize in Physics, 1930

Carotenoid molecules shift blue laser light colour to green:

from 473 nm to 510 nm

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2004 Data — 33,000 PeopleF

req

ue

ncy

LifePak

5-9 Fruits & Vegetables

Average (2-3 Fruits & Vegetables)Smokers

Obesity

LifePak + 5-9 Fruits & Vegetables

10,000 - 19,000

20,000 - 29,000

30,000 - 39,000

40,000 - 49,000

50,000+

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Knowing LifePak is working

LifePak Users vs. Non-usersAU & NZ

37,200

28,826

40,732

31,028

-

5,000

10,000

15,000

20,000

25,000

30,000

35,000

40,000

45,000

AU Never UsedLifePak

AU LifePakRegular Users

NZ Never UsedLifePak

NZ LifePakRegular Users

Av

era

ge

Sc

an

Sc

ore

31% higher29% higher

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2004 Data: Eat Fruits & Vegetables

31,100

28,560

24,773

21,981

10,000

15,000

20,000

25,000

30,000

35,000

1 or Less 2 to 3 4 to 5 6 or More

Reported Daily Fruit & Vegetable Servings

Ram

an

In

ten

sit

y, C

ou

nts

.

n = 9,336 n = 13,600 n = 6,916 n = 2,796

p < 0.01 p < 0.01 p < 0.01

2004 Data of 32,648 Non-Supplement Users

V. L

ow |

Low

| M

od.|

Hig

h

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Smoking

19890

13030

17600 17591

1305611593

0

5000

10000

15000

20000

25000

30000

Non-Smokers

Smokers(all)

PastSmokers

< 1/day 1-5/day > 5/day

n = 1047

n = 58

p < 0.01

n = 39 n = 10

n = 16

n = 32

2002 Data: Don’t Smoke!

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2004: Maintain Normal Body Weight!

29,236

25,612

20,835

16,603

10,000

15,000

20,000

25,000

30,000

35,000

< 25 25-29 30-39 > 40

Body Mass Index (BMI, kg/m2)

Ram

an

In

tesit

y, C

ou

nts

.

n = 11,588 n = 8,475 n = 422

p < 0.01 p < 0.01 p < 0.01

n = 4,550

2004 Data of 25,035 Subjects Overweight people need extra antioxidant protection through diet and supplementation.

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Accepted by the Scientific Community• New York Academy of Sciences, Feb 2003• Oxygen Club of California, Feb 2003 and Feb 2004• FASEB/American Society of Nutritional Sciences - April

2003 and April 2004• Gordon Research Conference, Jan 2004• Review paper published in Current Topics in Nutraceutical

Research, May 2004• American College of Nutrition, October 2004

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Skin Carotenoid ScaleBLUEBLUE 50,000+: Truly outstanding diet and lifestyle habits.

Long term. Excellent supplements, good genetics.

GREENGREEN 40,000 - 49,000: Excellent diets high in fruits & vegetables and excellent supplements.

YELLOWYELLOW 30,000 - 39,000: Better lifestyle. Healthy diet with fruits & vegetables and/or good supplements.

ORANGEORANGE 20,000 - 29,000: Average lifestyle

REDRED Under 19,000: Poor diet. Unhealthy lifestyle, no/ineffective supplements, poor genetics. Smoking?

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　　 Advice from The ExpertAdvice from The Expert

“Everyone should use the Pharmanex BioPhotonic Scanner to learn their optimal body defence score. Once you have your number, work to increase it and maintain it with a rich diet in fruits, vegetables and vitamin supplements.”

Dr.　Lester　PackerCNN　Money　09.17.02

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Motivation

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What is LifePak®?A comprehensive nutritional wellness program delivering the optimum amounts of all essential and generally beneficial nutrients for long-term health and well-being.

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LifePak® - Anti-ageing FormulaHealth Benefits

Optimum supplementation for general well-being

1. Addresses nutrient deficiencies2. Cardiovascular benefits3. Complete bone nutrition4. Promotes healthy immune function5. Supports normal blood sugar metabolism6. Protects against ageing symptoms7. Raises your antioxidant defence

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1. Nutrient Deficiencies

Percent of Adults Not Meeting RDA

0

10

20

30

40

50

60

70

80

90

Males

Females

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1. Nutrient Deficiencies

Percent of LifePak Users Not Meeting RDA

0

10

20

30

40

50

60

70

80

90

Males

Females

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Over 20 Antioxidants: protect blood lipids (LDL) from oxidation by free radicals

2. Cardiovascular Benefits

(means +/- SD)

42.1 4243.4

50.9

30

35

40

45

50

55

60

65

Start After 6 Weeks

PlaceboLifePak

p < 0.001

LifePak’s silent benefits: You don’t feel them at first, but you will appreciate them years later!

Oxidized LDL clingsto artery lining,causing plaque build-up

Artery

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Calcium & Magnesium: maintain normal blood pressure & heart rhythm

B-Vitamins: maintain normal (low) blood homocysteine (a risk factor for heart disease)

Flavonoids: promote blood circulation (platelet aggregation and microcirculation)

2. Cardiovascular Benefits Over 40 Antioxidants: protect blood lipids

(LDL) from oxidation by free radicals

40

0

5

10

15

20

25

30

35

40

45

50

0 20 40 60 80 100

Optimum Bone Mass

Typical Bone Mass

Osteoporosis Zone

Age (years)

Bon

e M

ass

3. Bone Nutrients

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Bone Mineralisation

US & EU: 23 million bone fractures per year due to osteoporosis = $27 billion medical costs

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3. Bone Nutrients• Calcium and magnesium are building blocks

of bone health and involved in development and maintenance of bone structure and strength.

• Vitamin D helps proper calcium absorption, and regulates calcium and magnesium bone metabolism.

• Vitamin K helps transport calcium to the bones.

• Vitamin A helps in the building of strong bones.

• Copper assists in maintaining normal bone strength.

• Zinc is involved in bone metabolism.

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4. Immune Function

Percent of Adults Not Meeting RDA

0

10

20

30

40

50

60

70

80

90 Males

Females

LifePak supports normal immune function• Vitamins C & E• Multi-Carotenoid Blend• Vitamin A, Vitamin B6 & Zinc

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LifePak promotes normal blood sugar, glucose tolerance and insulin metabolism

Chromium:– essential for normal insulin function– most diets are low in chromium (<50 mcg/day)

5. Blood Sugar & Insulin

Vitamin CVitamin EAlpha-Lipoic AcidMagnesiumZinc

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Spock, did somebodysay “Anti-ageing”?

Pharmanex has a logical approach

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LifePak Addresses a Major Cause of ageing:

DNA Damage• DNA (DeoxyriboNucleic Acid) is your

genetic blueprint • DNA enables healthy cell renewal into old

age

6. Ageing Symptoms

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DNA is damaged by free radicals, which are cell toxins:

A

Antioxidants “catch” free radicals before they cause damage

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Extent of DNA Damage73,000 DNA damage events per cell every day!

DNA repair enzymes fix most of the damage

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Reduce DNA Damage1. Reduce your exposure to free radicals!

don’t smoke avoid excessive sun exposure (use

sunscreens) avoid polluted areas

2. Improve your antioxidant defence! eat more fruits & vegetables take LifePak

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A

LifePak providesan arsenal of nutrients that fight DNA damage,

protect DNA, andenhance DNA repair

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LifePak & DNA Damage

LifePak Nutrient Function

Vitamin C &

Vitamin E

DNA damage DNA repair

Vitamin B6,

Folic Acid &

Vitamin B12

Essential for DNA synthesis, replication and repair

Zinc Stabilizes DNA DNA synthesis

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LifePak Nutrient Function

Multi-Carotenoids beta-carotene lycopene

lutein

DNA damage

Multi-Flavonoids

green tea catechins quercetin

grape seed extract citrus bioflavonoids

soy isoflavones

DNA damage DNA repair

-Lipoic Acid DNA damage

LifePak & DNA Damage

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Recent Study in Am. J. Clin. Nutr.

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How Can I Improve My Antioxidant and Anti-inflammatory protection?• Take Optimum Omega™ with LifePak®

– LifePak + Optimum Omega = Foundation– comprehensive benefits

– enhances absorption of LifePak®*

– 600-1,200 mg omega-3 fatty acids• 300-600 mg EPA• 200-400 mg DHA

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• Make sure all your meals contain antioxidants & phytonutrients!– Take LifePak + Optimum Omega with

your two main meals!– Include fruits & vegetables into your

other meal(s) and snacks!

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Live Better, Longer

Phar

man

ex

Prod

ucts

Solu

tions

And

Wei

ght M

gmnt

Optimal Nutrition - LifePak Family

Inflammation protection – Optimum Omega

Optimal Health

Free Radical Protection – LifePak Family

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Primary Benefits of LifePak Prime

OPTIMALLY FORMULATED FOR MEN OVER 40 ANDPOSTMENOPAUSAL WOMEN

1. Helps protect the body against symptoms of ageing 2. Protects cells with an advanced antioxidant network including

over 40 different antioxidants3. Promotes optimal bone health and maintains bone strength in

mature users 4. Promotes healthy immune function5. Supports normal blood sugar levels and healthy blood6. Supports a healthy cardiovascular system 7. Assists in brain functions8. Helps maintain eye function and health9. Assists in sexual function and energy production10. Corrects nutritional deficiencies

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Primary Benefits of LifePak Women

FORMULATED FOR PRE AND PERIMENOPAUSAL WOMEN

1. Corrects nutritional deficiencies2. Supplies an optimal level of bone nutrients and helps

prevent osteoporosis3. Promotes healthy immune function4. Supports normal blood sugar metabolism5. Supplies a comprehensive blend of nutrients to

support a healthy cardiovascular system6. Offers anti-ageing benefits and cell protection

against damaging free radical attacks by providing a powerful antioxidants network

7. Helps provide premenstrual syndrome symptom (PMS) relief

8. Assists in relief of menopause symptoms9. Helps prevent urinary tract infection (UTI)

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Jungamals® LifePak for Kids

• A comprehensive children’s multivitamin/mineral supplement

• A balanced bone support formula including calcium for strengthening bones

• Promotes healthy immune function with vitamin C, zinc, and other essential nutrients

• Provides a complete balance of vitamins optimally formulated to assist in important normal functions of building the body and utilising the body’s resources

• Provides chelated minerals magnesium, calcium, iron and zinc

• Chewable, great orange, berry & cherry flavours

It is LifePak for kids aged 2 and up!

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Fruit Phytonutrients

• At least 1,000 phytochemicals identified with cell protective effects

• Very few (25) studied• Fruits and vegetables – more than meets the eye• We don’t eat enough fruits and vegetables

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– R&D evaluated hundreds of fruits for…• Extraordinary antioxidant power (Scanner)• Extraordinary nutritional contents• Quality of scientific efficacy data• Impeccable safety data• History of traditional folklore usage

3 years ago…Pharmanex began Searching the World for Superfruits

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= gâc fruit + 3 superfruits= 3 years of Pharmanex research= 3 Pharmanex research labs1. Gâc fruit (Chinese Bitter Cucumber)2. Chinese Lycium (Wolfberry)3. Cili fruit 4. Siberian Pineapple (Seabuckthorn)

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Lipocarotenes™• Nature’s unique nutrient

delivery system in

• Gâc & Siberian Pineapple: – contain fatty acids (unique among

fruits)– with carotenoids & vitamin E pre-

dissolved– Gâc carotenes: 40 % improved

uptake**compared to beta-carotene powder. Vuong LT et al., Am J Clin Nutr 2002;75:872-879.

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ot âc?

New G3 Super Juice Coming in 2006New G3 Super Juice Coming in 2006

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