1. How many grams of sucrose (Mr 342) would you need in order

to make 500 mL of a 5 mM solution?

2. Assume a 36% (w/w) solution of protein (Mr 12 000) in water has a

density of 1.09 g/mL. What volume would you need in order to prepare 500 mL 200 μg/mL

solution?

1. 0.855 g

2. 0.25 mL

3. Calculate pH of 0.02 M NaOH.

4. Calculate pH when 25 mL 0.16 M NaOH is mixed with 50 mL 0.1

M HCl

5. What is the pH of a solution that is 0.4 M Na lactate and 0.2 M lactic acid? (pKa lactic acid =

3.73)

3. 12.3

4. 1.88

5. 4.03

6. Michaelis constant of the enzyme is 1 mM. At what concentration of

substrate will be the rate of reaction half of the maximum rate?7. The rate of reaction at maximum

saturation of enzyme with substrate was 500 μmol.s-1.

Michaelis constant of the enzyme is 1000 nM. What is the rate of

reaction at substrate concetration of 1 μM?

6. 1 mM

7. 250 μmol.s-1