1 dna and replication ubyirin84 dna replication…3 min. watch, listen and comprehend!!!!!!!
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DNA and ReplicationDNA and Replicationhttps://
www.youtube.com/watch?v=dKubyIRiN84
DNA replication…3 min.DNA replication…3 min.WATCH, LISTEN AND WATCH, LISTEN AND
COMPREHEND!!!!!!!COMPREHEND!!!!!!!
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https://www.youtube.com/watch?v=iHKNbhgaylgDNA REPLICATION SONG!!!!
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Discovery of DNA Discovery of DNA StructureStructure
• Erwin Chargaff showed the amounts of the four bases on DNA ( A,T,C,G)
• In a body or somatic cell: A = 30.3% T = 30.3% G = 19.5% C = 19.9%
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Chargaff’s RuleChargaff’s Rule• AdenineAdenine must pair with
ThymineThymine• GuanineGuanine must pair with
CytosineCytosine• The bases form weak
hydrogen bonds
G CT Acopyright cmassengale
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DNA StructureDNA Structure•Rosalind Franklin took
diffraction x-ray photographs of DNA crystals
• In the 1950’s, Watson & Crick built the first model of DNA using Franklin’s x-rays
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Rosalind FranklinRosalind Franklin
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DNA DNA StructurStructur
ee
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DNADNA•Two strands coiled called
a double helix•Sides made of a pentose
sugar Deoxyribose bonded to phosphate (PO4) groups by phosphodiester bonds
•Center made of nitrogen bases bonded together by weak hydrogen bonds
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DNA Double HelixDNA Double Helix
NitrogenousNitrogenousBase (A,T,G or C)Base (A,T,G or C)
““Rungs of ladder”Rungs of ladder”
““Legs of ladder”Legs of ladder”
Phosphate &Phosphate &Sugar BackboneSugar Backbone
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HelixHelix
• Most DNA has a Most DNA has a right-right-handhand twist with twist with 10 base 10 base pairspairs in a complete turn in a complete turn
• Left twisted DNA is called Left twisted DNA is called Z-DNAZ-DNA or or southpawsouthpaw DNA DNA
• Hot spotsHot spots occur where occur where right and left twisted right and left twisted DNA meet producingDNA meet producing mutationsmutations
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DNADNA•Stands for
Deoxyribonucleic acid•Made up of subunits
called nucleotidesnucleotides • NucleotideNucleotide made of: made of:
1. Phosphate groupPhosphate group2. 5-carbon sugar5-carbon sugar3. Nitrogenous baseNitrogenous base
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DNA NucleotideDNA Nucleotide
O=P-O O
PhosphatePhosphate GroupGroup
NNitrogenous baseNitrogenous base (A, G, C, or T)(A, G, C, or T)
CH2
O
C1C4
C3 C2
5
SugarSugar(deoxyribose)(deoxyribose)
O
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Pentose SugarPentose Sugar• Carbons are numbered
clockwise 1’ to 5’CH2
O
C1C4
C3 C2
5
SugarSugar(deoxyribose)(deoxyribose)
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DNADNA
P
P
P
O
O
O
1
23
4
5
5
3
3
5
P
P
PO
O
O
1
2 3
4
5
5
3
5
3
G C
T A
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Antiparallel Antiparallel StrandsStrands
• One strand of DNA goes from 5’ to 3’ (sugars)
• The other strand is opposite in direction going 3’ to 5’ (sugars)
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Nitrogenous Nitrogenous BasesBases
• Double ring Double ring PURINESPURINESAdenine (A)Adenine (A)Guanine (G)Guanine (G)
• Single ring Single ring PYRIMIDINESPYRIMIDINES
Thymine (T)Thymine (T)Cytosine (C)Cytosine (C) T or C
A or G
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Base-PairingsBase-Pairings•Purines only pair with
Pyrimidines•Three hydrogen bonds
required to bond Guanine & Cytosine
CG
3 H-bonds
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T A
•Two hydrogen bonds are required to bond Adenine & Thymine
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Question:Question:
•If there is 30% AdenineAdenine, how much CytosineCytosine is present?
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Answer:Answer:•There would be 20%
CytosineCytosine• Adenine (30%) = Adenine (30%) =
Thymine (30%)Thymine (30%)• Guanine (20%) = Guanine (20%) =
Cytosine (20%)Cytosine (20%)• Therefore, Therefore, 60% A-T 60% A-T
and 40% C-Gand 40% C-Gcopyright cmassengale
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DNA DNA ReplicatiReplicati
onon
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Replication FactsReplication Facts
• DNA has to be copied DNA has to be copied before a cell dividesbefore a cell divides
• DNA is copied during DNA is copied during the the SS or synthesis phase or synthesis phase of of interphaseinterphase
• New cells will need New cells will need identical identical DNA strandsDNA strands
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Synthesis Phase (S Synthesis Phase (S phase)phase)
• S phase during interphase of the cell cycle
• Nucleus of eukaryotes
Mitosis-prophase-metaphase-anaphase-telophase
G1 G2
Sphase
interphase
DNA replication takesDNA replication takesplace in the S phase.place in the S phase.
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DNA ReplicationDNA Replication
• Begins atBegins at Origins of ReplicationOrigins of Replication• Two strands open forming Two strands open forming
Replication Forks (Y-shaped Replication Forks (Y-shaped region)region)
• New strands grow at the forksNew strands grow at the forks
ReplicationReplicationForkFork
Parental DNA MoleculeParental DNA Molecule
3’
5’
3’
5’copyright cmassengale
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DNA ReplicationDNA Replication
• Enzyme Enzyme HelicaseHelicase unwinds and separates unwinds and separates the 2 DNA strands by the 2 DNA strands by breaking the breaking the weak weak hydrogen bondshydrogen bonds
• Single-Strand Binding Single-Strand Binding ProteinsProteins attach and keep the 2 DNA strands separated and untwisted
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DNA ReplicationDNA Replication• Enzyme Enzyme TopoisomeraseTopoisomerase
attaches to the 2 forks of the bubble to relieve stressrelieve stress on the DNA moleculeDNA molecule as it separatesEnzyme
DNA
Enzyme
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DNA ReplicationDNA Replication• BeforeBefore new DNA strands can
form, there must be RNA RNA primersprimers present to start the addition of new nucleotides
• PrimasePrimase is the enzyme that synthesizes the RNA Primer
• DNA polymerase can then add the new nucleotides
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DNA ReplicationDNA Replication• DNA polymeraseDNA polymerase can only add can only add
nucleotides to the nucleotides to the 3’ end3’ end of of the DNA the DNA
• This causes the This causes the NEWNEW strand to strand to be built in a be built in a 5’ to 3’ direction5’ to 3’ direction
RNARNAPrimerPrimerDNA PolymeraseDNA Polymerase
NucleotideNucleotide
5’
5’ 3’
Direction of ReplicationDirection of Replicationcopyright cmassengale
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Remember HOW the Remember HOW the Carbons Are Numbered!Carbons Are Numbered!
OO=P-O O
PhosphatePhosphate GroupGroup
NNitrogenous baseNitrogenous base (A, G, C, or T)(A, G, C, or T)
CH2
O
C1C4
C3 C2
5
SugarSugar(deoxyribose)(deoxyribose)
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Remember the Strands are Remember the Strands are AntiparallelAntiparallel
P
P
P
O
O
O
1
23
4
5
5
3
3
5
P
P
PO
O
O
1
2 3
4
5
5
3
5
3
G C
T A
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Synthesis of the New Synthesis of the New DNA StrandsDNA Strands
• The The Leading StrandLeading Strand is synthesized as a single single strand strand from the point of origin toward the opening replication fork
RNARNAPrimerPrimerDNA PolymeraseDNA PolymeraseNucleotidesNucleotides
3’5’
5’
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Synthesis of the New DNA Synthesis of the New DNA StrandsStrands
• The The Lagging StrandLagging Strand is is synthesized discontinuouslydiscontinuously against overall direction of replication
• This strand is made in MANY short segments It is replicated from the replication fork toward the origin
RNA PrimerRNA Primer
Leading StrandLeading Strand
DNA PolymeraseDNA Polymerase
5’
5’
3’
3’
Lagging StrandLagging Strand
5’
5’
3’
3’ copyright cmassengale
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Lagging Strand Lagging Strand SegmentsSegments
• Okazaki FragmentsOkazaki Fragments - - series of short segments on the lagging strandlagging strand
• Must be joined together by Must be joined together by an an enzymeenzyme
Lagging Strand
RNARNAPrimerPrimer
DNADNAPolymerasePolymerase
3’
3’
5’
5’
Okazaki FragmentOkazaki Fragment
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Joining of Okazaki Joining of Okazaki FragmentsFragments
• The enzyme The enzyme LigaseLigase joins the joins the Okazaki fragments together Okazaki fragments together to make one strandto make one strand
Lagging Strand
Okazaki Fragment 2Okazaki Fragment 2
DNA ligaseDNA ligase
Okazaki Fragment 1Okazaki Fragment 1
5’
5’
3’
3’
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Replication of Replication of StrandsStrands
Replication Fork
Point of Origin
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https://www.youtube.com/watch?v=vNXFk_d6y80DNA replication…leading and lagging strand..2:38Watch and listen!!!!
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Proofreading New Proofreading New DNADNA
• DNA polymerase initially DNA polymerase initially makes about makes about 1 in 10,0001 in 10,000 base base pairing errorspairing errors
• EnzymesEnzymes proofread and proofread and correct these mistakescorrect these mistakes
• The new error rate for DNA The new error rate for DNA that has been proofread is that has been proofread is 1 1 in 1 billionin 1 billion base pairing errors base pairing errors
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Semiconservative Model Semiconservative Model of Replicationof Replication
• Idea presented by Idea presented by Watson & CrickWatson & Crick• TheThe two strands of the parental
molecule separate, and each acts as a template for a new complementary strand
• New DNA consists of 1 PARENTAL (original) and 1 NEW strand of DNA
Parental DNA
DNA Template
New DNA
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DNA Damage & DNA Damage & RepairRepair
• Chemicals & ultraviolet radiation damage the DNA in our body cells
• Cells must continuously repair DAMAGED DNA
• Excision repair occurs when any of over 50 repair enzymes remove damaged parts of DNA
• DNA polymerase and DNA ligase replace and bond the new nucleotides together
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Question:Question:
•What would be the complementary DNA strand for the following DNA sequence?
DNA 5’-CGTATG-3’DNA 5’-CGTATG-3’copyright cmassengale
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Answer:Answer:
DNA 5’-CGTATG-3’DNA 5’-CGTATG-3’
DNA 3’-GCATAC-5’DNA 3’-GCATAC-5’
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https://www.youtube.com/watch?v=27TxKoFU2NwDNA replication process. 3D animation. 5 min…..7 facts!!!