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1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management Department, Sanitary Eng. Group Vakantiecursus, TUDelft, January 2014

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Page 1: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

1Challenge the future

Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge

Mostafa Zahmatkesh, CiTG, Water Management Department, Sanitary Eng. GroupVakantiecursus, TUDelft, January 2014

Page 2: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

2Challenge the future

Project Outline

Wastewater Treatment EffluentS

lud

ge

Humics

Lignin

Page 3: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

3Challenge the future

Recalcitrant Compounds

Lignin Humics

Page 4: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

4Challenge the future

White rot fungi

Lignin Degrading Enzymes

Lignin Degradation

Lignocellulose Degradation

Lignin

Cellulose

Hemicellulose Lignin Degrading Enzymes

Page 5: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

5Challenge the future

Challenge: Non-sterile conditions!

Page 6: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

6Challenge the future

Approach

• Fungal Strain Prescreening

• Inoculation Method

• Bioreactor

• Process design

Page 7: 1 Challenge the future Development of fungal bioreactors for Treatment of Lignin/Humics- rich wastewater and sludge Mostafa Zahmatkesh, CiTG, Water Management

7Challenge the future

Challenges:

• Limiting bacterial growth and increasing the fungal

growth

• Inoculation strategy in continuous mode

• Maintaining short HRT and long fungal SRT in continues

run with optimum degradation activity


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