0dwhuldo (6, iru1dqrvfdoh 7klv supporting informationtaiwan university, 1, section 4, roosevelt...
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Supporting Information
Photoluminescent Graphene Quantum Dots for in vivo
Imaging of Apoptotic Cells
Prathik Roy,a Arun Prakash Periasamy,a Chiu-Ya Lin,b Guor-Mour Her,b Wei-Jane
Chiu,b Chi-Lin Li,a Chia-Lun Shu,a Chih-Ching Huang,b* Chi-Te Liang,c,d and Huan-
Tsung Changa*
aDepartment of Chemistry, National Taiwan University, Taipei, Taiwan; bDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; cDepartment of Physics, National Taiwan University, Taipei, Taiwan; dGeballe Laboratory for Advanced Materials (GLAM), Stanford University, Stanford, CA 94305, USA;
E-mail: [email protected]
Correspondence: Professor Huan-Tsung Chang, Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan; Tel. and fax: 011-886-2-3366-1171; e-mail: [email protected]
Professor Chih-Ching Huang, Institute of Bioscience and Biotechnology, National Taiwan Ocean University, 2, Pei-Ning Road, Keelung 20224, Taiwan. Tel.: 011-886-2-2462-2192 ext. 5517; E-mail: [email protected].
Electronic Supplementary Material (ESI) for Nanoscale.This journal is © The Royal Society of Chemistry 2015
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Quantum Yield equation:
, Ф = Ф𝑅
𝐼𝐼𝑅
𝐸𝑅
𝐸𝑛2
𝑛2𝑅
where Ф denotes quantum yield, I is integrated fluorescence intensity, E is extinction
co-efficient, n= refractive index and the index R indicates the standard.
Figure S1. XRD spectrum of the as-prepared GQDs.
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Figure S2. (A) UV-visible absorption spectrum and (B) excitation dependent
emission spectra (from 365 to 485 nm) of GQDs (2 mg mL−1) prepared in DI water.
Inset: Photograph of photoluminescent GQD solution (excited at 365-nm).
Absorbance (Abs) in (A) and PL intensity (IPL) are plotted in arbitrary units (a. u.).
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Figure S3. Mortality (n = 12, three replicates, where n is the number of embryos) of
zebrafish embryos exposed in GQD solutions (20 ng mL−1 2 mg mL−1) in the period
of 0–72 h.
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Figure S4. Time course recording of morphology of zebrafish embryos exposed in
GQD solutions (0–2 mg mL−1) in the period of 0–72 h.
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Figure S5. Morphologies of zebrafish without (up row) and with (bottom row)
treatment with GQD solutions (10 mg mL−1) for 120 hpf.
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Figure S6. Cell viability of HeLa, MCF-7 and MCF-10A cells after GQDs (20 ng
mL−12 mg mL−1) treatment for 4 h. The results are the mean + SD of three repeated
experiments.