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DNA Films as Model Polyelectrolytes and As Platforms for Biomolecular Diagnostics Rastislav Levicky, Columbia University, DMR- 0093758 Interfaces modified with charged polymers arise in applications as diverse as colloidal processing and biosensing, yet many aspects of their physical behavior remain puzzling. Monolayers of DNA, an anionic polyelectrolyte, are under study to determine how electrostatic and other forces govern their self-assembly and organization. This past year, DNA polymer brushes were investigated with electrochemical impedance spectroscopy (EIS) to learn about the ionic environment inside these films, and the results compared with theoretical prediction. In Top. Capacitance (C) of a polyelectrolyte (DNA) brush as a function of monovalent ionic strength and surface coverage of chains. Bottom. Near-field microwave imaging of a DNA-monolayer (spots) in the dry state; extension to allow imaging in liquids is in progress (collaboration with K. Lee, Sogang 0.8 mm 0.01 0.1 1 6.0 7.0 8.0 C ( F/cm 2 ) NaCl(M ) increasing DN A coverage D N A brush - - - - - - - - - - tip DNA layer

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DNA Films as Model Polyelectrolytes and As Platforms for Biomolecular Diagnostics Rastislav Levicky, Columbia University, DMR-0093758. - PowerPoint PPT Presentation

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DNA Films as Model Polyelectrolytes and As Platforms for Biomolecular Diagnostics

Rastislav Levicky, Columbia University, DMR-0093758

Interfaces modified with charged polymers arise in applications as diverse as colloidal processing and biosensing, yet many aspects of their physical behavior remain puzzling. Monolayers of DNA, an anionic polyelectrolyte, are under study to determine how electrostatic and other forces govern their self-assembly and organization. This past year, DNA polymer brushes were investigated with electrochemical impedance spectroscopy (EIS) to learn about the ionic environment inside these films, and the results compared with theoretical prediction. In collaboration with colleagues, new tools to characterize such systems with near-field imaging methods were also developed. J. Am. Chem. Soc. (2005), 127, 9666. Langmuir (2004), 20, 9621.J. Am. Chem. Soc. (2004), 126, 9910.

Top. Capacitance (C) of a polyelectrolyte (DNA) brush as a function of monovalent ionic strength and surface coverage of chains. Bottom. Near-field microwave imaging of a DNA-monolayer (spots) in the dry state; extension to allow imaging in liquids is in progress (collaboration with K. Lee, Sogang U., and B. Friedman, Sam Houston State U.).

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Education and High School Outreach:We are fortunate to have had a number of excellent participants contribute to this project through summer research including high school science teachers (Youning Wang, Murry Bergtraum HS; Robert Nociti, George Washington HS; Ann Meyer, New Expl. Sci. Tech. HS), high school students (Alice Jia, Pei Da Guo, Heejae Kim), and undergraduates (Ana Acosta, Agnes Yeboah, Dan Okin, Susannah Dickerson, Nardine Zakhary). The project has also provided the main thesis focus for graduate students Dr. Patrick Johnson (now at Rutgers U.) and Gang Shen, as well as lecture material for the course Biopolymers taught by the P.I.

DNA Films as Model Polyelectrolytes and As Platforms for Biomolecular Diagnostics

Rastislav Levicky, Columbia University, DMR-0093758

Prof. Levicky with summer 2005 research participants. From left: high school student Heejae Kim (project: DNA brushes on colloids), Prof. Levicky, high school science teacher Ann Meyer (project: formulation of liquid glass coatings), and undergraduate student Nardine Zakhary (project: chemistry of silicone gel films).


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