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(DMB 01)

M.Sc. (Previous) DEGREE EXAMINATION,

JUNE 2010.

First Year

Microbiology

Paper I INTRODUCTION MICROORGANISMS

Time : Three hours Maximum : 100 marks

PART A (5 8 = 40 marks)

Answer any FIVE of the following.

1. Spontaneous generation.

2. Louis Pasteur.

3. Cyanobacteria.

4. Mycoplasma.

5. Chemistry of viruses.

6. Viroids.

7. Protozoa significance.

8. Fungi reproduction.

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(DMB 01)2

PART B (4 15 = 60 marks)

Answer all FOUR questions.

9. (a) Elaborate on the developments of vaccines.

Or(b) Outline the ultra structure of typical

bacterial cell.

10. (a) Explain the principles of bacterial taxonomy.

Or

(b) Write an essay on Agrobacterium and its

importance.

11. (a) Describe the discovery of important viruses.

Or

(b) Outline the ultrastructure and replication

of T4.

12. (a) What is Ainsworth system and how it is

useful in classifying fungi?

Or

(b) Discuss the structure and replication of

microalgae.

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(DMB 02)

M.Sc. (Previous) DEGREE EXAMINATION, JUNE 2010.

First Year

Microbiology

Paper II MICROBIOLOGICAL METHODS


PART A (5 8 = 40 marks)


1. Simple staining.

2. Ethylene oxide.

3. Bailing technique.

4. MPN method.

5. Plaque assay.

6. Protoplast method.

7. Beers Lambert Law.

8. Autoradiography.

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(DMB 02)2

PART B (4 15 = 60 marks)

Answer ALL questions.

9. (a) Describe the electron microscope and its

uses.

Or

(b) Elaborate on the physical methods used for

sterilization.

10. (a) How to isolate pure culture using the plating

methods?

Or

(b) Discuss the methods of anaerobic culturing.

11. (a) Explain the methods used for isolation and

purification of viruses.

Or

(b) What are chromatographic methods and

discuss the HPLC?

12. (a) Write an essay on liquid scintillation

chromatography.

Or

(b) Outline the pulse field gel electrophoresis

and its uses.

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(DMB 03)


JUNE 2010.

First Year

Microbiology

Paper III MICROBIAL PHYSIOLOGY AND

BIOCHEMISTRY


SECTION A (5 8 = 40 marks)

Answer any FIVE of the following :

All questions carry equal marks.

1. Faciliated diffusion.

2. Batch culture.

3. Purple sulfur bacteria.

4. Iron Oxidizers.

5. Free energy.

6. Kreb's cycle.

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(DMB 03)2

7. Mixed fermentation.

8. Purine.

SECTION B (4 15 = 60 marks)

Answer all the FOUR questions.

All questions carry equal marks.

9. (a) Discuss the role of macronutrients and

micronutrients on growth.

Or

(b) Explain the factors affecting bacterial

growth.

10. (a) Describe the mechanism of photosynthesis in

anoxygenic bacteria.

Or

(b) Explain the characteristics and importance

of Nitrate oxidizers and sulfur oxidizers.

11. (a) Describe the mechanisms of phosphorylation.

Or

(b) Explain the steps involved in Glycolysis.

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(DMB 03)3

12. (a) Describe Michaelis - mention equation and

mechanism of enzymes catalysis.

Or

(b) Explain the structure of Watson - Crick's

model of DNA.

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(DMB 04)


JUNE 2010.

First Year

Microbiology

Paper IV ENVIRONMENTAL AND

AGRICULTURAL MICROBIOLOGY


SECTION A (5 8 = 40 marks)


1. Phytoplankton.

2. Air sampling.

3. Soil components.

4. Rhizosphere.

5. Leg haemoglobin.

6. Cron induction groups.

7. Blast of Rice.

8. Soft rot of Vegetables.

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(DMB 04)2

SECTION B (4 15 = 60 marks)

Answer ALL questions.

9. (a) Describe the waterborne diseases.

Or

(b) Elaborate the role of Aero biology in relation

to plant pathology.

10. (a) Discuss the soil organic matter

decomposition.

Or

(b) How the carbon in transformed in the

environment?

11. (a) Explain the symbiotic nitrogen fixation.

Or

(b) What are the types of Mycorliza and discuss

the importance of VAM fungi?

12. (a) Outline the symptoms caused by plant

pathogens.

Or

(b) Write an essay on biological control of plant

pathogens.

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