What is it?

What are they?

What is it? How does it work?

DNA is isolatedDNA is copied with

PCRCut with restriction

enzymesRun through charged

gel boxCompared

What are they? How are they made?

PCR makes lots of copies of the geneChosen gene is cut with restriction

enzymes, leaving “sticky ends”Circular bacterial DNA is cut with same

restriction enzymes, leaving “sticky ends”Cut pieces are joined together, so bacteria

now produces protein in gene