contents · the term “3” indicates the position of the carbonyl group, ... other natural...
TRANSCRIPT
Contents Abstract: .................................................................................................................................................. 1
Chapter 1: Introduction: ......................................................................................................................... 2
1.1 3(2H)-Furanones: Structure and Nomenclature .................................................................... 2
1.2 Bioactive 3(2H)-Furanones ..................................................................................................... 3
1.3 Synthesis and Functionalization of 3(2H)-Furanones ........................................................... 6
1.4 Introduction of Fluorine at the C4 Position of 3(2H)-Furanones. ....................................... 10
1.5 Application of Organofluorine Compounds in Medicinal Chemistry ................................. 11
1.6 Project Aims and Objectives ................................................................................................ 12
Chapter 2: Results and Discussion ........................................................................................................ 13
2.1 Overview of the Synthetic Route to the Target Compound ............................................... 13
2.2 Synthesis of 3-hydroxy-3-methyl-2-butan-2-one 15 ........................................................... 14
2.3 Synthesis of 4-hydroxy-4-methyl-1-(4’-bromophenyl)-1-penten-3-one 28 ....................... 16
2.4 Synthesis of 4,5-dibromo-2-hydroxy-2-methyl-5-(4’-bromophenyl)-3-pentanone 29 ...... 18
2.5 Synthesis of 2,2-dimethyl-5-(4’-bromophenyl)-3(2H)-furanone 30 ................................... 21
2.6 Synthesis of 2,2-dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31 .................... 27
2.7 Conclusions ........................................................................................................................... 29
2.8 Further Work ........................................................................................................................ 29
Chapter 3: Experimental ....................................................................................................................... 31
3.1 Synthesis of 3-hydroxy-3-methyl-2-butan-2-one 15 ........................................................... 31
3.2 Synthesis of 4-hydroxy-4-methyl-1-(4’-bromophenyl)-1-penten-3-one 28 ....................... 31
3.3 Synthesis of 4,5-dibromo-2-hydroxy-2-methyl-5-(4’-bromophenyl)-3-pentanone 29 ...... 32
3.4 Synthesis of 2,2-dimethyl-5-(4’-bromophenyl)-3(2H)-furanone 30 ................................... 32
3.5 Synthesis of 2,2-dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31 .................... 32
Acknowledgement: .................................................................................. Error! Bookmark not defined.
References ............................................................................................................................................ 33
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Abstract: The 3(2H)-furanone moiety is found in a variety of natural products and molecules with
bioactivity. Consequently, robust methods for the synthesis and functionalization of 3(2H)-
furanones are required in order to efficiently synthesise these molecules. Currently, there
are a number of methods which allow for robust and versatile functionalization of the C2
and C5 positions of the 3(2H)-furanone ring but functionalization of the C4 position is largely
limited to substitution reactions involving halogens as leaving groups. There are a number of
electrophilic substitutions which are known at this position but the range of known suitable
substrates for vinyl nucleophilic substitutions at this position is extremely limited.
Of particular interest is the incorporation of fluorine at the C4 position of the 3(2H)-
furanone. Organofluorine compounds have a number of useful properties which make the
study of their synthesis desirable. It has been shown that electrophilic sources of fluorine
incorporate readily at the C4 position but there has been little success in incorporating
fluorine via vinyl nucleophilic substitution. Theoretically, 2,2-dimethyl-4-bromo-5-(4’-
bromophenyl)-3(2H)-furanone would be an ideal compound upon which to carry out vinyl
nucleophilic substitutions of nucleophilic substitution.
The work detailed in this report sought to examine an established synthesis of 2,2-dimethyl-
4-bromo-5-(4’-bromophenyl)-3(2H)-furanone and identify opportunities for improvement of
that synthetic route to this compound.
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Chapter 1: Introduction:
1.1 3(2H)-Furanones: Structure and Nomenclature
3(2H)-Furanones are ketonic oxygen-containing five membered heterocycles of the general
structure shown in Figure 1.1.1 (a). The term “3” indicates the position of the carbonyl group,
while “2H” refers to the site of hydrogenation from the parent furan (Figure 1.1.1 (b)). The
unsubstituted 3(2H)-furanone (Figure 1.1.1 (c)) is also known to be isolable.
Figure 1.1.1 (A) General structure of a 3(2H)-furanone showing the ring numbering system, (B) Furan and (C)
3(2H)-furanone.
Initially, interest in 3(2H)-furanones arose from the discovery of the sesquiterpene
bullatenone 1 by Brandt, Thomas and Taylor1 which has been the subject of many synthetic
investigations due to its fragrance enhancement and cockroach repellent properties2. Later,
other natural compounds containing the 3(2H)-furanone moiety were isolated including the
monoamine oxidase inhibitor geiparvarin 2 and pseurotin A 3.
Figure 1.1.2 (1) Bullatenone (2) Geiparvarin and (3) Pseurotin A.
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As the above structures in figure 1.1.2 highlight, there are a variety of substitution patterns
which can be found on the central 3(2H) structure including the spiro-fused pattern of
pseurotin A 3, a known antiparasitic compound3.
1.2 Bioactive 3(2H)-Furanones
An array of bioactive 3(2H)-furanones have been discovered. Geiparvarin 2 is known to inhibit
the enzyme monoamine oxidase and as such was originally investigated for its potential to
act as an anti-depressant4, however it has since been found that geiparvarin demonstrates
potent cytotoxic activity and much of the work which has been completed on geiparvarin and
its analogues to date has been in examining its potential as an anti-cancer agent5,6. SAR
experiments have demonstrated that the 3(2H)-furanone moiety is essential for the biological
activity of geiparvarin and its analogues7. As such, most of the bioactive analogues are
functionalised at the coumarin moiety only.
2,5-dimethyl-4-hydroxy-3(2H)-furanone (DMHF) 4 is a compound found in a number of foods
and is known to exhibit antimicrobial effects on human pathogenic microorganisms including
clinically isolated antibiotic-resistant strains8. DMHF represents an ideal drug candidate due
to its simple structure, high potency and wide range of action. Its physicochemical properties
would suggest that DMHF would be readily bioavailable. Partition coefficient (Log POW)
calculations using Chem3D Pro© forecast its Log POW to be ca. 1.13, indicating that it is
lipophilic enough to cross cell membranes. DMHF is likely to be soluble in water also due to
the availability of carbonyl and hydroxyl groups to become involved in hydrogen bonding
interactions 5 represented in figure 1.2.1.
Figure 1.2.1 (4) Structure of DMHF and (5) its likely hydrogen bonding sites.
Other compounds of note include the class of spiro-fused 3(2H)-furanones reported in 1993
by the Du Pont-Merck group9 which contain an appended aromatic group at C5 of the
furanone ring and also a cycloalkyl ring at C2 containing a tertiary amine as represented by 6
in figure 1.2.2. A number of compounds of this type demonstrated selective competitive
antagonist behaviour at sigma receptors suggesting potential use as antipsychotic drugs. Two
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compounds, aryl-substituted spirofuranones 7 and 8 represented in figure 1.2.2, in particular
demonstrated potent affinity but their development as drug compounds was discontinued
due to their toxicity profile9.
Figure 1.2.2 (6) General structure of 3(2H)-furanones with sigma receptor affinity (7,8) Examples of the most
potent of this class of compound.
A final example of bioactive 3(2H)-furanones are the 4,5-diaryl 3(2H)-furanone class. These
compounds were designed based on the initial observation that cis-1,2-diaryl alkenes were
selective inhibitors of the COX-2 enzyme once one of the aromatic rings contained a para-
sulphone group 9 shown in figure 1.2.3. In fact, many of the currently approved COX-2
selective NSAIDs (e.g. Etoricoxib 10 and Celecoxib 11 also figure 1.2.3) contain this sulphone
cis-1,2-diaryl alkene moiety.
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Figure 1.2.3 (9) General structure of bioactive sulphone-containing cis-1,2-diaryl alkenes (10) Etoricoxib (11)
Celecoxib
Achieving COX-2 selectivity is a key priority in the development of new anti-inflammatory
drugs as leading compounds in the Non-Steroidal Anti-Inflammatory (NSAID) class (e.g.
Aspirin, Indomethacin, and Diclofenac) also inhibit the COX-1 isoform. COX-1 is responsible
for many physiological functions necessary for regular function (homeostasis) and as such its
inhibition results in a number of side-effects including difficulty in forming blood clots and
adverse gastric effects.
3(2H)-Furanone analogues of 9 were synthesised as outlined in Shin et al.10. Compounds of
type shown in figure 1.2.4 were found to have a selectivity of ca. 1000-fold for COX-2 over
COX-1.
Figure 1.2.4 Examples of COX-2 selective inhibitors of the 3(2H)-Furanone class.
The relevance of the above examples lies in their demonstration of the variety of compounds
in which the 3(2H)-furanone moiety can be found, highlighting the importance of developing
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methods of synthesising and functionalising 3(2H)-furanones for drug development and total
synthesis purposes.
1.3 Synthesis and Functionalization of 3(2H)-Furanones
A number of methods for synthesis of 3(2H)-furanones are reported in the literature. Early
syntheses, such as that reported by Takeda et al.11 generated the 3(2H)-furanone skeleton by
way of the disconnection shown in Scheme 1.3.1.
Scheme 1.3.1 Primary disconnection used in Takeda’s synthesis of substituted 3(2H)-furanones.
Dibromide 14 can be prepared from the anti-bromination of hydroxyenone 17 a product of
the Claisen-Schmidt condensation of α-hydroxyketone 15 and aldehyde 16 as in Scheme
1.3.2.
Scheme 1.3.2 Synthesis of hydroxyenone 17 used in the preparation of dibromide 14
This synthesis allows for robust functionalization of the product 3(2H)-furanone. For instance,
replacing the methyl groups at the alpha position of the ketone in 15 would enable an array
of C2 substituted 3(2H)-Furanones. One such way in which this could be exploited is in the
synthesis of spiro-fused 3(2H)-furanones of the type represented by 6 as per figure 1.2.2.
Using this methodology, α-hydroxyketone 18 would act as a suitable reagent and could be
generated using an adapted version of the approach used to synthesise spiro-fused furanones
employed by Schow and Tam9 as in Scheme 1.3.3.
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Scheme 1.3.3 – Preparation of the advanced spiro-fused α-hydroxyketone intermediate 18 for the synthesis of
spiro-fused 3(2H)-furanones using an adapted approach.
Similarly, altering the structure of aldehyde 15 would afford a variety of substitution patterns
at C5. As the formation hydroxyenone 17 is achieved through Claisen-Schmidt type chemistry
which is known to be reliably reproducible for an array of substrates.
Functionalisation of the C4 position of the 3(2H)-Furanone ring in this method relies on
exploiting the ability for C4 to undergo vinyl nucleophilic substitution reactions. The most
commonly used strategy is to use an electrophilic halogenating agent such as N-
Chlorosuccinimide (NCS) or N-Bromosuccinimide (NBS) to halogenate the C4 position and
then carry out further substitution, shown in Scheme 1.3.4.
Scheme 1.3.4 Functionalization of the C4 position of 3(2H)-furanones by way of electrophilic halogenating agents
There are two principal disadvantages to using the approach employed by Takeda et al.
1) As this is a multi-step process, there are considerable opportunities for loss of yield
and some of the reactions may require long periods of time to complete.
2) Synthetically useful α-hydroxyketone starting materials may not be readily available
and as such, additional synthetic steps may be necessary to prepare them.
To address these issues, multiple strategies have emerged which seek to shorten the length
of the synthesis, improving yield. A variety of one-step cyclisation reactions have been
developed in the past 10 years. These reactions are usually catalysed by Au(III) or Pt(II)
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complexes although base catalysed variants without metal involvement exist also. The
substrates vary in nature but generally include an alkyne neighbouring a carbon with oxygen
bound to it.
In 2010, Egi et al. 12 reported the gold-catalysed reaction shown in Scheme 1.3.5.
Scheme 1.3.5 – Egi et al. synthesis of substituted 3(2H)-furanones.
This methodology was used to synthesise a variety of C2 and C5 substituted 3(2H)-furanones,
examples of which 19-23 are given below in Figure 1.3.1. These reactions are by and large
high yielding and relatively quick. However, as demonstrated in figure 1.3.1, the yield of these
reactions diminishes considerably when C2 is unsubstituted (R1, R2 = H) as for 23.
Figure 1.3.1 Selected products of Egi et al.’s 3(2H)-furanone synthesis. The yield for each compound is included
in parentheses.
Liu et al.’s13 earlier 2006 gold-catalysed synthesis of substituted 3(2H)-furanones is apparently
the earliest synthesis of this type reported in the literature. As shown in Scheme 1.3.6, alkyne-
substituted α-ketoesters cyclise in the presence of alcohols and AuCl3 to form C2-acetal
derivatives of 3(2H)-furanones with the option C5 functionalization.
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Scheme 1.3.6 – Liu et al.’s synthesis of C2-acetal functionalised 3(2H)-furanones
This method is much lower yielding than Egi et al.’s method but the variety of substrates
which can be used makes this a much more robust synthetic approach.
One-step cyclisations which functionalise C4 directly are also possible. Trofimov et al.’s 2010
synthesis14 of C4-nitrile derivatives demonstrates this as shown in Scheme 1.3.7.
Scheme 1.3.7 Trofimov et al.’s Synthesis of C4-nitrile derivatives of 3(2H)-Furanones
This reaction is not known to work for substituents other than nitriles. This limits the
usefulness of this transformation as nitriles are not particularly efficient leaving groups. This
largely limits the chemistry which can be done at C4 to functional group transformations of
the nitrile, such as the acid catalysed transformation of nitriles to carboxylic acids or reaction
with DiBAL-H to form aldehydes. Aldehyde functionalization at C4 adds an additional
electrophilic centre to the molecule which can allow it to undergo Wittig-type chemistry or
react with nucleophiles. Another important reaction which can be carried out on this centre
is the formation of tetrazoles from 1,3-dipolar additions of azides. This type of chemistry is
widely practiced in the pharmaceutical industry, largely because the tetrazole group acts as a
bioisostere of carboxylic acids.
Although these one-step reactions appear attractive initially, they also present problems.
1) The starting materials for these reactions are difficult to prepare and are not generally
commercially available.
2) Metal catalysts increase the cost of synthesis, limiting their use.
3) Many of these reactions have been difficult to reproduce.
4) Takeda et al.’s facilitates the incorporation of a variety of functional groups into the
synthetic plan.
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A combination of these factors and the historical prevalence of Takeda et al’s synthesis has
contributed greatly to the popularity of their approach despite the existence of these one-
step methods.
1.4 Introduction of Fluorine at the C4 Position of 3(2H)-Furanones.
As discussed previously, the functionalization of C2 and C5 of the 3(2H)-furanone is
substantially easier to achieve than the functionalization of C4. As previously noted in Scheme
1.3.4, C4 functionalization can be achieved by halogenating C4 and then substituting the
halogen for another group. Of particular interest to the host research group is the fluorination
of 3(2H)-furanones at C4.
Work previously described15 demonstrates that Selectfluor®, a source of electrophilic
fluorine, can efficiently fluorinate C4 of 3(2H)-furanones. This is generally achieved through
the synthesis of 4-bromo-3(2H)-furanone 24 which is reacted in the presence of Selectfluor®
to give 4-fluoro-3(2H)-furanone 25 as per Scheme 1.4.1.
Scheme 1.4.1 Fluorination at C4 from 4-bromo-3(2H)-furanone 24 is achieved using Selectfluor®
Further, it has also been demonstrated 15 that it is possible to produce 25 using Selectfluor®
directly without proceeding via the 4-bromo-3(2H)-furanone 24 as shown in Scheme 1.4.2.
Scheme 1.4.2 Direct fluorination to form 25
The systems described in Scheme 1.4.1 and Scheme 1.4.2 respectively employ the use of
electrophilic fluorine. Using 4-bromo-3(2H)-furanone 24, it should be hypothetically possible
to substitute fluorine into the molecule by using a nucleophilic source, as in vinyl nucleophilic
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substitution. Successful vinyl nucleophilic substitutions at C4 of 3(2H)-furanones have been
accomplished using nucleophiles such as – CN 16 and it may be possible to carry out
substitution using the fluoride anion. In principal, demonstrating that fluoride can be
incorporated into the C4 of a 3(2H)-furanone system by nucleophilic means would show that
it is at least plausible for other vinyl systems. The ability to incorporate fluorine into organic
molecules by both electrophilic and nucleophilic means greatly increases the versatility of
planned syntheses of fluorinated compounds.
1.5 Application of Organofluorine Compounds in Medicinal Chemistry
Many licenced drug molecules, examples of which can be seen in figure 1.5.1, contain fluorine
in order to exploit its unique properties17.
Figure 1.5.1 Examples of licenced APIs which contain fluorine in their structure. (a) Emtricitabine – a
Nucleoside Reverse Transcriptase Inhibitor (b) Linezolid – an antibiotic and (c) 5-Fluorouracil – a
potent cytotoxic chemotherapeutic drug.
Drug-Design: The carbon-fluorine bond has a number of unique and important physical
properties which make its inclusion in organic molecules, particularly pharmaceutical
compounds, quite attractive. Firstly, it is a known bioisostere of hydrogen, allowing fluorine
to be introduced into positions previously occupied by hydrogen with no increase in steric
bulk. This is particularly important for compounds whose mechanism of action involves
interacting with the active site of enzymes for which steric bulk is an important consideration
as the molecule must successfully enter the active site.
Additionally, the carbon-fluorine bond is a polar bond, owing to the electronegative nature of
fluorine. This allows for the introduction of polarity into organic compounds without the need
to incorporate bulky hydroxyl groups. This is important for the reasons relating to steric bulk
previously described, but also, it offers the opportunity to remove hydrogen bond acceptors
like hydroxyl groups which are an important factor which influences the bioavailability of drug
molecules.
It is also important to note that despite the polarity of the carbon-fluorine bond, the bond is,
resistant to hydrolytic18, 19 and oxidative metabolic activity20, 21. It can also be demonstrated
that the inclusion of fluorine has an effect on the pKa of neighbouring functional groups. The
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perturbation of pKa can strongly modify the binding affinity and the pharmacokinetic
properties of a pharmaceutical agent. Modulation of pKa may impact on bioavailability by
affecting the absorption process of drugs across cell membranes based on their degree of
ionisation at physiological pH 22, 23, 24.
18F-labelled Radiopharmaceuticals: 18F is a radioactive isotope of fluorine and as such can be
detected by its β-decay pattern25. Compounds such as 18F-Fluorodeoxyglucose are routinely
used for diagnostic purposes in the detection of cancerous masses. Imaging using this
technique is only possible because the C-18F bond is not metabolically cleaved in-vivo as
otherwise the metabolites would contribute to background radiation, reducing the sensitivity
of the measurement26.
The use of 18F compounds for PET scans is also well established 27, 28. 18F is widely used for PET
scans as it can be imaged easily, requiring smaller doses than other radionuclides. It also has
a longer half-life than other radionuclides at 109.7 minutes. This provides a sufficient amount
of time for these labelling molecules to be synthesised off-site and brought to hospitals which
don’t have access to the facilities to produce them on-site29.
A potential application of the incorporation of 18F-labelled compounds is the evaluation and
monitoring of in vitro distribution of labelled drug molecules in whole tissue and organ testing
models. The ability to determine the distribution of a drug molecule would provide invaluable
insight into the prediction of in vivo toxicokinetics, pharmacokinetics and mechanism of
action.
1.6 Project Aims and Objectives
Given the previous analysis of the importance of studying the functionalization of C4 of the
3(2H)-furanone system, the work described in this report sought to investigate the synthesis
of 2,2-dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31 using the synthetic approach
employed by Takeda et al. The 3(2H)-furanone 31 is an ideal substrate for the examination of
the substitution reactions of nucleophilic fluorine at the C4 position of 3(2H)-furanones and
as such can act as a model for the further study of vinyl nucleophilic substitutions of fluoride.
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Chapter 2: Results and Discussion
2.1 Overview of the Synthetic Route to the Target Compound
The method used to synthesise the target compound 2,2-dimethyl-4-bromo-5-(4’-
bromophenyl)-3(2H)-furanone 31 is depicted in Scheme 2.1.1. The five-step synthesis starts
with the hydration of 2-methyl-3-butyn-2-ol 26 to form 3-hydroxy-3-methyl-2-butan-2-one 15.
3-hydroxy-3-methylbutan-2-one 15 is then condensed with p-bromobenzaldehyde 27 in an
aldol-type condensation form 4-hydroxy-4-methyl-1-(4’-bromophenyl)-1-penten-3-one 28.
The alkene group in 4-hydroxy-4-methyl-1-(4’-bromophenyl)-1-penten-3-one 28 is then
brominated forming the anti-dibromide compound 4,5-dibromo-2-hydroxy-2-methyl-5-(4’-
bromophenyl)-3-pentanone 29 which is cyclised in the presence of base forming the 3(2H)-
furanone 2,2-dimethyl-5-(4’-bromophenyl)-3(2H)-furanone 30. Finally, 2,2-dimethyl-5-(4’-
bromophenyl)-3(2H)-furanone 30 is brominated using to N-bromosuccinimide to 2,2-
dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31.
Scheme 2.1.1 Formation of 4-bromo-3(2H)-furanone 31
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2.2 Synthesis of 3-hydroxy-3-methyl-2-butan-2-one 15
The hydration of 2-methyl-3-butyn-2-ol 26 was achieved using mercuric oxide in a solution of
sulphuric acid and water as per Scheme 2.2.1.
Scheme 2.2.1 Synthesis of 3-hydroxy-methylbutan-2-one 15
The mechanism of this step is outlined in Scheme 2.2.2. The reaction is initiated when the
mercury associates with the π-electrons of the alkyne as in 32 forming a transient three-
membered ring species which undergoes a Markovnikov addition of water 33 to form the enol
34. The enol 34 undergoes tautomerisation to the protonated ketone 35 which forms the enol
36 upon the loss of Hg(II). The enol 36 undergoes a final tautomerisation to form the ketone
15.
The product of this step of the reaction was obtained as a black oil which was purified upon
distillation at atmospheric temperature to yield 48.2g (48%) of the clear, colourless oil.
This step of the reaction is quite low yielding. There are a number of reasons as to why this
might be the case.
1) Compound 15 is likely to be considerably soluble in water. Therefore, the aqueous
washes which are used in the work-up of this compound would remove product with
them also. This may be avoided by carrying out washes with brine, saturating the
water, minimising the solubility of the ketone.
2) Compound 15 is a low boiling liquid and therefore may evaporate during the
distillation or be lost during the heating stages of the reaction.
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The progress of this reaction was followed by monitoring the formation of the carbonyl group,
indicated by the appearance of a triplet at ca. 2.25 ppm integrating for 3H using 1H NMR as
shown in figure 2.2.1. Three distinct hydrogen environments were observed in the spectrum
for this compound which are also noted in figure 2.2.1.
Figure 2.2.1 Assignment of 1H NMR spectrum for ketone 15
This reaction could also be potentially be monitored by in-situ FITR spectroscopy by following
the formation of the carbonyl group based on the appearance of an absorption of at ca. 1650
cm-1.
It is at this stage of the process that functionality at the C2 position of the 3(2H)-furanone is
introduced. Altering the structure of the alkyne used at this stage could lead to a variety of
different compounds.
2.3 Synthesis of 4-hydroxy-4-methyl-1-(4’-bromophenyl)-1-penten-3-one 28
The synthesis of the hydroxyenone 28 was accomplished by condensing ketone 15 with p-
bromobenzaldehyde via an aldol condensation as shown in Scheme 2.3.1. As there is only one
enolisable hydrogen environment in this system, this type of chemistry is more accurately
referred to as a Claisen-Schmidt reaction.
Scheme 2.3.1 Synthesis of hydroxyenone 28
The mechanism of this reaction is outlined in Scheme 2.3.2. Enolate 37 is formed by
deprotonating the alpha position of ketone 15 which then attacks the carbonyl of p-
bromobenzaldehyde 27 forming the anionic tetrahedral intermediate 38 which becomes
protonated in solution to form the alcohol 39. The position alpha to the ketone in 38 is
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deprotonated allowing for the irreversible elimination of water, forming the α,β-unsaturated
enone 28 which is promoted by the formation of the conjugated system.
Scheme 2.3.2 Mechanism for the condensation for ketone 15 and p-bromobenzaldehyde 17 to form enone 28.
This reaction preferentially forms the E-isomer rather than the Z-isomer largely for steric
reasons. The configuration at this point is confirmed by examining the coupling constants of
the protons on either end of the alkene as shown in figure 2.3.1. The coupling constants
observed were ca. J = 15 Hz corresponding to the E-configuration expected. The orientation
of these two protons with respect to each other can be confirmed using the Karplus
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equation should appropriate background work be done, establishing the constants required.
Crystallographic data would also confirm this configuration.
Figure 2.3.1 Assignment of chemical shifts to both of the alkene hydrogens, highlighting their coupling constants
in hertz.
The progress of this reaction was monitored by TLC. It was noted that the reaction was
complete after 15 hours. This was determined by monitoring the disappearance of the p-
bromobenzaldehyde 27, of which a commercially purchased sample was available. After an
acid workup, a yellow waxy solid was obtained which was then recrystallized from
DCM/hexane to yield a yellow crystalline solid (8.3g, 87% yield).
This reaction presents another opportunity to utilise in-situ FITR spectroscopy. Given that the
product enone 28 is highly conjugated, a shift in the carbonyl absorbance (to about 1700-
1720 cm-1) from that which is observed in either of the two parent carbonyl compounds could
be detected.
It is at this stage at which the functionality at C5 of the 3(2H)-furanone product is introduced.
p-bromobenzaldehyde was chosen for this reaction because the resulting enone was
predicted to be a crystalline solid where other analogues of lower molecular weight tend to
be waxy solids or oils. This allows for the easier purification by crystallisation rather than the
use of column chromatography.
2.4 Synthesis of 4,5-dibromo-2-hydroxy-2-methyl-5-(4’-bromophenyl)-3-pentanone
29
The next stage in the synthesis of the 3(2H)-furanone is the formation of the anti-dibromide
compound 29, shown in Scheme 2.3.1. This transformation was accomplished by the addition
of bromine across the alkene double bond, forming the anti-dibromide.
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Scheme 2.3.1 Formation of the anti-dibromide 29
The mechanism for this reaction is given in Scheme 2.3.2. The π-electrons of the alkene 28
attack one of the bromine atoms in dibromine, releasing electron density onto the second
bromine forming the syn-bromonium species 40 and bromide. Bromide then adds to the
opposite face of the ring, forming the anti-bromide 29.
Scheme 2.3.2 Mechanism for the formation of dibromide 29
In reality, the opposite stereochemical configuration of the intermediate 40 is equally likely
to be observed, shown in Scheme 2.3.3 as compound 41. This would then undergo attack by
the bromide in the same manor, again shown in Scheme 2.3.3, yielding 42 which is an
enantiomer of 29.
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Scheme 2.3.3 Formation of diastereomer 42
As these compounds are enantiomers, they are expected to have similar physical properties.
The dibromide was not purified before processing to the next step of the reaction as it is
known that the crude intermediate can be forward processed without difficulty.
Crystallographic data would confirm the presence of enantiomers and demonstrate if there
was any enantioselectivity achieved in the course of the reaction.
The loss of conjugation in this step presents another opportunity to measure the progress of
this reaction by either IR or in-situ FITR. As indicated in the previous section, the presence of
the alkene group in conjugation with the ketone causes a shift in the observed absorption of
the carbonyl group. Upon loss of conjugation, it would be expected that the absorbance of
the carbonyl group would return to ca.1650 cm-1.
As highlighted in figure 2.3.1, 1H NMR data suggests the formation of the anti-compound as
the coupling constants for the two hydrogens at the sp2 carbons is ca. 12 Hz. It also interesting
to note that the hydrogens bound to the carbon in the alpha position with respect to ketone
are no longer magnetically equivalent. This is due to those protons now being enatiotopic.
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Figure 2.3.1 Spectral Assignment of dibromide 29
2.5 Synthesis of 2,2-dimethyl-5-(4’-bromophenyl)-3(2H)-furanone 30
The cyclisation of the dibromide 29 to form the furanone 30, represented in Scheme 2.4.1,
was achieved by stirring an ethanolic solution of the dibromide 29 in the presence of sodium
hydroxide overnight. Despite acknowledging previously that both 29 and 41 are produced in
the previous step, both of these compounds are known to undergo this reaction and as such
only 29 will be discussed.
Scheme 2.4.1 Synthesis of 3(2H)-furanone 30 from dibromide 29
A plausible mechanism for this step is given in Scheme 2.4.2. In this mechanism, the hydroxyl
group in 29 becomes deprotonated and attacks the benzylic carbon in an SN2 type manor,
expelling bromide and forming intermediate 42. Due to the reversal of stereochemistry, the
proton is now located anti-periplanar to the bromine on C4 of the ring and can undergo E2
type elimination yielding the 3(2H)-furanone 30.
22 | P a g e
Scheme 2.4.2 The currently accepted mechanism for the formation of 3(2H)-furanone 30
Another plausible mechanism would involve the reaction progressing via an SN1 type
mechanism as per Scheme 2.4.3. In this scheme, the bromine at the benzylic position of 29 is
ejected from the molecule forming the cationic intermediate 43 which undergoes
intramolecular nucleophilic attack yielding the pair cationic diastereomers 44 and 45 which
become deprotonated by either the hydroxide or bromide anions to yield 46 and 42, the
previously described intermediate of the suggested SN2 reaction mechanism.
23 | P a g e
Scheme 2.4.3 A possible SN1 mechanism for the formation of 3(2H)-furanone 30
As the conformation of the hydrogen with respect to bromine in both 46 and 42 is fixed
relative to one another, the type of elimination reaction which proceeds is likely different for
the two substrates. Bromide 46 has the bromine leaving group and the hydrogen orientated
24 | P a g e
syn-periplanar to one another and thus cannot undergo an E2 type elimination. It is therefore
likely that it undergoes an E1 type elimination, via the formation of the cationic intermediate
47, to yield the product 3(2H)-furanone 30. Conversely, bromide 42 has its bromine leaving
group and the hydrogen orientated anti-periplanar to one another and can undergo E2 type
elimination to form the product 3(2H)-furanone directly.
This mechanism is at least plausible as the reaction is carried out in ethanol, a polar protic
solvent, which is known to promote SN1/ E1 reactions due to its ability to stabilise the
development of the positive charge in the rate-determining transition state. It is also worth
noting that the formation of benzylic carbocations is possible due to the resonance stabilising
effect of the aromatic ring.
The SN2 reaction mechanism appears attractive on first glance because the deprotonation of
the alcohol presents a readily available nucleophile. However, it is unlikely that complete
deprotonation occurs at the alcohol. Utilising Chem3D Pro©, the approximate pKa of this
proton is ca. 16.8. The pKa of water, the conjugate acid of hydroxide, is approximately 15.
Therefore it is unlikely that complete deprotonation of the alcohol occurs. This presents an
opportunity for the formation of the carbocation, which is sufficiently electrophilic to be
attacked by the unprotonated alcohol.
The formation of carbocationic intermediates may be an issue in this reaction and could
explain low yields. This reaction is completed in the presence of ethanol and water, both of
which have the capacity to act as nucleophiles, presenting the opportunity to form impurities
containing hydroxyl and ethoxide side-chains.
Understanding the mechanism of this reaction provides the opportunity to rationally consider
new reaction conditions and protocols to optimise this step, discussed in the “Further Work”
section of this report. A number of experiments could be carried out to determine (a) which
mechanism is predominant under these conditions and (b) the relative contributions of these
mechanisms to the overall process.
1) Isolating intermediates 42 and 46.
It might be possible to isolate intermediates 42 and 46 and to characterise them using NMR
spectroscopic and crystallographic methods. It is possible that isolation could be achieved by
using a sub-stoichiometric amount of base or by otherwise insuring that the reaction doesn’t
progress to the elimination stage. NMR spectroscopy would allow you to distinguish the
relative position of the benzylic proton and the proton at the alpha position of the ketone by
examining their 1H-3J coupling constants and comparing them with the values predicted by
the Karplus equation for the appropriate dihedral angles. It is anticipated that both of these
25 | P a g e
compounds would form crystalline solids due to their high molecular mass and thus
crystallography could be used to solve for their absolute stereochemistry.
The presence of 46 would indicate that the reaction proceeds, at least in part, via the SN1
pathway.
2) Using kinetic isotope effects
Should the presence of 46 be confirmed, the relative contribution of both pathways could be
estimated by studying the kinetic isotope effect observed on replacing the benzylic proton
with deuterium. Deuteration could be easily achieved by using the deuterated analogue of
p-bromobenzaldehyde 48 shown in figure 2.4.1 instead of the undeuterated analogue for the
reaction described in Scheme 2.3.1.
Figure 2.4.1 Deuterated analogue of p-bromobenzaldehyde
If the reaction proceeds via the SN1 mechanism as opposed to the SN2 mechanism, a
secondary kinetic isotope effect would be observed where KH/KD would be ca. 1.2-1.6. Were
the reaction to proceed via the SN2 mechanism instead, it is unlikely that a kinetic isotope
effect would be observed.
The feasibility of this approach is dependent on determining a way to arrest the reaction at
the formation of intermediates 42 and 46 as determining the mechanism using isotope effects
alone becomes more complicated once the reaction progresses to the elimination phase. This
is because both E1 and E2 reactions are subject to isotope effects. E2 reactions are subject to
a large primary kinetic isotope effect as the rate determining step involves the removal of
deuterium directly where E1 reactions are subject to a much smaller secondary isotope as
deuterium is not involved in the rate determining step but does stabilise the carbocation
intermediate. The SN1 pathway allows for E1 elimination as well as E2 elimination as
previously stated, therefore this complicates the interpretation of the observed kinetic
isotope effect.
The above considerations are important in determining opportunities to optimise the both
the product yield and time taken to complete the reaction. The yield of 3(2H)-furanone 30
from this reaction was 73% requiring 18 hours stirring time. Improving either of these two
26 | P a g e
parameters is important as 3(2H)-furanone 30 is an important substrate upon which further
investigations are carried out.
The progress of the reaction was monitored, using 1 H NMR, based on the appearance of a
signal corresponding to the vinyl proton at ca. 5.90 ppm, as shown in figure 2.4.2. TLC was
not used to monitor this step as no authentic sample of the dibromide compound 29 was
available for analysis. Also, as can be seen in figure 2.4.2, the protons corresponding to the
methyl groups at C2 of the 3(2H)-furanone ring are magnetically equivalent once more as they
are no longer enantiotopic.
Figure 2.4.2 Assignment of noteworthy peaks for 3(2H)-furanone 30.
The product obtained from this step after the initial workup was observed to be a waxy yellow
solid. This compound is normally purified by column chromatography but this is known to be
a considerable cause of yield loss for this step. As such, an alternative method of purifying
this compound was sought. Trituration was investigated as a potential alternative to column
chromatography. It was found that triturating the solid in a solvent system of 40% ether in
hexane purified the sample to >98%. The pure compound was obtained as a yellow feathery
solid.
Trituration takes advantage of the solubility differences of the product of interest and its
impurities in a solvent mixture. The presence of impurities causes melting point depression
and is responsible for causing reaction products to form oils, gums and waxes when they
would otherwise be crystalline solids. In order for trituration to be successful, it is necessary
to develop an appropriate solvent system in which the crude compound is sparingly soluble
but in which the impurities are highly soluble. The crude product is then suspended in the
solvent mixture and the stirred for a length of time. The suspended solids are then filtered,
27 | P a g e
yielding the purified compound, while impurities remain in the mother liquors. If necessary,
multiple crops of the mother liquor can be carried out to improve yield.
HSQC (Heteronuclear Single Quantum Coherence) and HMBC (Heteronuclear Multiple-Bond
Correlation) spectroscopy were carried out on the purified compound to confirm the
assignment of the compound prior to commencement of the next reaction.
2.6 Synthesis of 2,2-dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31
The synthesis of the bromofuranone 31 was accomplished by reaction of 3(2H)-furanone 30
with N-Bromosuccinimide (NBS) 49, a source of electrophilic bromine as shown in Scheme
2.5.1.
Scheme 2.5.1 Synthesis of bromofuranone 31 using N-Bromosuccinimide.
The mechanism for this reaction is given in Scheme 2.5.2. The NBS used in this reaction was
not pure NBS, but rather trace hydrogen bromide was present. This is important as it initiates
the release of bromine radicals for the bromination step. Initially, NBS 49 deprotonates HBR
forming the quaternary amide 50. The bromide released in this step attacks the N-bromine
releasing electron density onto the quaternary nitrogen producing the acetimide 51 which
tautomerises to form succinimide 52 as well as dibromine. Dibromine then polarises to form
two bromine radicals. One of these radicals attacks the 3(2H)-furanone 29 at the vinyl position
forming intermediate 53. The second bromine radical produced scavanges the hydrogen from
the vinylic position producing the bromofuranone 31 as well as regenerating HBr.
When this transformation was carried out using recrystallised NBS, the reaction was very
slow. A mixture of compounds were obtained which were identified as the unsubstituted
3(2H)-furanone 30 and some of the 4-bromo compound 31. It was found that these
compounds, despite being similar in structure, can be separated efficiently using a 20% ethyl
acetate in hexane eluent system.
Dry DCM, which was stored over 4A molecular sieves, was used as the solvent for this
reaction. Water which is present in the reaction mixture has been observed to cause the
formation of mono- and bis-hydroxy-3(2H)-furanone analogues.
28 | P a g e
Scheme 2.5.2 Mechanism for the synthesis of bromofuranone 31.
This reaction was carried out on a 1 mmol scale in CDCl3 intitally to predict the rate of reaction
by ReactNMR. It was hoped that the progress of the reaction could be followed by the rate of
loss of the vinyl proton of 3(2H)-furanone 30 however it transpired that the reaction occurred
instaneously upon mixing. The reaction was then repeated, adding the NBS portionwise
during which time a transient wine-red colour was observed. This colour is unlikely to be due
to the formation of hydrogen bromide as it is a colourless gas.
A prominent issue when using NBS as a source of electrophilic bromine is the succinimide 51
which is produced as a by-product. It is difficult to separate the succinimide from the product
without using carbon tetrachloride, a solvent which is no longer used routinely due to its
status as a possible carcinogen. Consequently, an alternative method for the removal of the
succinimide was invesitgated.
The NBS reaction was repeated once more using 2.67 g (10 mmol) of 3(2H)-furanone 30 and
the products of the reaction were worked up as normal. Initially, it was attempted to remove
the succinimide from the product by using trituration with cold ether. 1H NMR confirmed that
29 | P a g e
this managed to remove approximately 80% of the succinimide on the first. In order to
remove the remaining succinimide, an appropriate eluent system was investigated for use in
column chromatography. Varying concentrations of EtOAc/Hexane were examined using TLC
of which a 10% ethyl acetate in hexane mixture provided the most pronounced separation,
leaving the succinimide at the baseline.
The product from the 10 mmol scale reaction was then purified by column chromatography
yielding 0.8g (42%) of white solid. 1H NMR was used to confirm complete removal of the
furanone
2.7 Conclusions
- The titular compound 2,2-dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31 was
synthesised and characterised by NMR.
- There is scope for examination of the cyclisation mechanism for formation of the
unsubstituted 3(2H)-furanone 30 which could lead to optimisation of yield and reaction rate.
- There is considerable scope for the inclusion of modern spectroscopic equipment such as
ReactIR and ReactNMR for the purpose of monitoring the early stages of the reaction.
- Trituration is a convenient and effective technique for the purification of both the
unsubstituted 30 and 4-bromo substituted 31 3(2H)-furanones and may in time be able to
replace Column Chromatography as the method of choice.
- Trituration is also a convenient method for the removal succinimide after the NBS reaction.
2.8 Further Work
Project 1: Vinyl Nucleophilic Substitutions
Given that the 4-bromo compound 31 has been successfully synthesised and purified, the
next step would be to carry out the vinyl nucleophilic substitutions using sources of fluorine.
Initially it would make sense to use KF or other forms of anionic fluorine. In the case that this
doesn’t work, alternative sources of fluorine could be used.
If necessary, 4-iodo derivatives could be used. Iodine is a better leaving group than bromine
and this might promote attack at the 4-position. These may be prepared using N-
iodosuccinimide or perhaps via Finkelstein reaction chemistry.
Nucleophilic catalysis could also be employed. Carrying out the reaction in the presence of an
amine nucleophile, such as pyridine or DMAP (N,N-dimethylaminopyridine) may result in rate
enchancements.
It would also be useful to examine additional nucleophiles which could be used to
functionalise this position. In particular; alcohol and amine analogues may be worth exploring
for 1) bioactivity and 2) functionalization purposes.
30 | P a g e
Project 2: Furanone Cyclisation Optimisation
As argued in section 2.4, there is a case to be made for the cyclisation mechanism to proceed
via an SN1 type mechanism rather than the SN2 mechanism which has been broadly asserted
previously. As outlined in section 2.4, a number of experiments could be carried out,
exploiting kinetic isotope effects and crystallography, to determine if there is a contribution
by the SN1 pathway.
Understanding if the development of positive charge in the mechanism is significant as yield
and rate enhancements may be obtained by designing reaction conditions which either
promote or discourage this charge formation. This may allow for better control over the
formation of side-products.
Project 3: Reactivity of 3(2H)-Furanone C4 Halides
While the focus of the work identified in the project has been on the potential for substitution
reactions to occur on the halides at C4 of the 3(2H)-furanone, it is possible for them to have
other reactivity. It might be useful to examine transition metal catalysed reactions using the
4-halogenated substrates.
Project 4: Further Investigation into the Removal of Succinimide.
Trituration proved to be an effective method for the removal of succinimide from the 4-
bromo compound. It would be useful for further work on this compound to develop a protocol
for the removal of succinimide which can be routinely used.
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Chapter 3: Experimental Note: All chemical reagents, unless specifically synthesised in the course of this work, were
commercially available. Melting points were measured on a Thomas-Hoover Capillary
melting point apparatus.
NMR spectra were recorded in deuteriochloroform (CDCl3) with tetramethylsilane (TMS) as
internal standard unless indicated otherwise. 1H NMR spectra were recorded at 300 MHz on
a BRUKER AVANCE 300 spectrometer.
Thin layer chromatography (TLC) was carried out on pre-coated silica gel 60 (Merck PF254)
plates. Column chromatography was carried out on Merck PF 254 silica gel 60. Solvents were
distilled prior to use according to standard procedures by persons in the lab.
As all of these compounds are known compounds, IR Spectroscopy, Mass Spectroscopy and
Elemental Analysis were not carried out.
3.1 Synthesis of 3-hydroxy-3-methyl-2-butan-2-one 15
2-methyl-3-butyne-2-ol 26 (97 ml, 1 mol) was added over 40 minutes to a solution of yellow
mercuric oxide (13 g, 0.06 mol) in concentrated H2SO4 (18 ml, 0.34 mol) and water (100 ml),
while maintaining the temperature below 70°C. Once the addition was complete, the reaction
was heated to 70°C and refluxed overnight. The product was filtered through a bed of Celite®
under vacuum, and the Celite® washed with diethyl ether (3 x 20 ml). The filtrate was
extracted with ether (2 x 30 ml) and washed with water (1 x 50 ml) and saturated NaHCO3
solution (1 x 40 ml) and dried using sodium sulphate. The resulting dark oil was distilled at
atmospheric pressure to yield (52.71g, 52% yield) of clear colourless oil.
δH (ppm): 1.41 (6H, s, 2 x CH3), 2.25 (3H, s, C(O)CH3), 3.83 (1H, s, OH)
3.2 Synthesis of 4-hydroxy-4-methyl-1-(4’-bromophenyl)-1-penten-3-one 28
3-hydroxy-3-methyl-2-butanone 15 (5 g, 49 mmol) and p-bromobenzaldehyde 27 (9.02g, 49
mmol) were dissolved in 95% ethanol (30 ml) with KOH (3 pellets) and allowed to stir
overnight as the clear colourless solution became cloudy and yellow. The solution was
acidified with 10% HCl to pH 1 upon which it became clear and yellow. The solvent was
removed by distillation under reduced pressure at 60°C. This yielded an orange residue which
was dissolved in ethyl acetate (40 ml) and washed with water (3 x 30 ml) and brine (1 x 50
ml). The solution was dried over sodium sulphate, filtered by gravity and concentrated under
reduced pressure to yield an orange solid. The solid was then recrystallized from DCM/hexane
yielding the yellow crystalline solid (10.28g, 78%)
δH (ppm): 1.47 (6H, s, C(CH3)2), 7.09 (1H, d, J = 15 Hz, O=C-CH), 7.42-7.43 (3H, m, ArH),
7.59-7.62 (2H, m, ArH), 7.79 (1H, d, J=15 Hz, CH-Ar).
32 | P a g e
3.3 Synthesis of 4,5-dibromo-2-hydroxy-2-methyl-5-(4’-bromophenyl)-3-pentanone
29
Neat bromine (2.520 g, 15.8 mmol) was added dropwise to a stirred solution of 4-hydroxy-4-
methyl-1-(4’-bromophenyl)-pentene-3-one 28 (4.25 g, 15.8 mmol) in DCM (40 ml). The
reaction was allowed stir for 1 hour. The solvent was concentrated under reduced pressure
to give an orange oil which solidified on cooling (5.28 g, 78.1%%, m.p. 114-115°C).
δH (ppm): 1.54 (3H, s, C(OH)CH3), 1.63 (3H, s, C(OH)CH3), 2.52-2.59 (1H, bs, OH), 5.42 (1H, d,
J=11.7 Hz, HCBrAr), 5.67 (1H, d, J = 11.7 Hz, O=CHCBr), 7.29-7.44 (5H, m, ArH).
3.4 Synthesis of 2,2-dimethyl-5-(4’-bromophenyl)-3(2H)-furanone 30
Potassium hydroxide (4.719 g, 0.0401 mol, 2 equivalents) was added to a solution of 4,5-
dibromo-2-hydroxy-2-methyl-5-(4’-bromophenyl)-3-pentanone 29 (8.62 g, 0.02 mol) in 95%
ethanol (120 ml) and the solution stirred overnight. The brown solution was acidified with 10
% HCl to pH 1 and then concentrated under reduced pressure. The solution was extracted
into ethyl acetate (2 x 30 ml) and washed with water (2 x 30ml). The solution was dried over
sodium sulphate and filtered by gravity, the sodium sulphate cake washed with ethyl acetate
(1 x 20 ml), and the filtrate was concentrated under reduced pressure to give an orange waxy
solid which upon trituration with 40% ether in hexane, was isolated as a white crystalline solid
(4.2 g, 79.3% yield, m.p. 109°C).
δH (ppm): 1.50 (6H, s, C(CH3)2), 5.98 (1H, s, C=CH) 7.49-7.63 (3H, m, m- & p- ArH), 7.83 (2H, d,
o-ArH).
3.5 Synthesis of 2,2-dimethyl-4-bromo-5-(4’-bromophenyl)-3(2H)-furanone 31
N-bromosuccinimide (1.8g, 10 mmol) was added portion wise (over approx. 15 minutes) to a
solution of 2,2-dimethyl-5-(4’-bromophenyl)-3(2H)-furanone 30 (2.67g, 10 mmol) in DCM (30
ml) on ice. The reaction mixture was allowed to stir for 30 minutes before being washed with
saturated NaHCO3 (1 x 20 ml) and water (3 x 20 ml). The solution was then dried using sodium
sulphate, filtered and the filtrate concentrated under reduced pressure to give a white
crystalline solid. The crude product was purified by column chromatography using 10% ethyl
acetate in hexane as an eluent, the solvent was removed by distillation under reduced
pressure to give the product as a white solid (1.45 g, 41.8%, m.p. 73-75°C).
δH (ppm): 1.57 (6H, s, C(CH3)2), 7.50-7.63 (3H, m, m- & p- ArH), 8.19-8.22 (2H, m, o-ArH).
33 | P a g e
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