+ production of engineered long-life and male-sterile pelargonium plants
TRANSCRIPT
![Page 1: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/1.jpg)
+
Production of Engineered long-life and male-sterile Pelargonium
plants
![Page 2: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/2.jpg)
+ Key Words
-- Pelargonium zonale and Pelargonium peltatum
-- pSAG12 and PsEND1 promoter
-- Engineered long-lived and male sterile plants
-- ipt gene: expressed in long-living plants
-- Barnase: gene expressed in pollen free plants
-- Agrobacterium tumefaciens
-- Delayed senescence
-- Anther ablation: abnormalities in anther production
Click icon to add picture
Click icon to add picture
![Page 3: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/3.jpg)
+
Background
-- Flowering plant
-- Grown in Europe since 17th century
-- More than 200 species of Pelargonium
--Cultivars: P. zonale and P. peltatum is the most popular.
-- Species of Pelargonium are considered important in:
• Market of ornamental plants• Perfume industry
![Page 4: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/4.jpg)
+
Pelargonium Breeds
Background
![Page 5: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/5.jpg)
+Pelargonium breeders produce new flower colors, shapes and market quality plants via gene transfer
Long-living plants
Allergen-Free house plants
Click icon to add picture
Click icon to add pictureClick icon to add picture
![Page 6: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/6.jpg)
+-Agrobacterium tumefaciens
-- Crown Gall Disease
-- Important in gene transfer
Plant Manufacturers
![Page 7: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/7.jpg)
+-- In vitro regeneration system
-- Pelargonium zonale explant used as a starting materials.
-P. zonale – organogenesis - shoots
-Two new traits in P. zonale-Long-life plants- Male sterility
-Promoter used:-pSAG12 – Senescence-specific gene promoter-PsEND1 promoter – Anther specific promoter
Click icon to add picture
Click icon to add picture
Regeneration of plants
![Page 8: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/8.jpg)
+ Plant senescence-- Study of aging in plants
-- pSAG12 promoter fuses with ipt gene which has an ipt enzyme-- Produce more cytokinin
-- Delayed Senescence
![Page 9: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/9.jpg)
+Influence of pSAG12:ipt
![Page 10: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/10.jpg)
+Male Sterile Plants
-Why are they important?
- eliminates pollen allergens
- Prevents gene flow of GM material and related plant species.
Click icon to add picture
Click icon to add picture
![Page 11: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/11.jpg)
+Elimination of pollen allergens
Expression of barnase
gene
- Anther – specific PsEND1
promoter
-Specific ablation in
plants
Click icon to add picture
Click icon to add picture
-Anther tissues are necessary
- No pollen grains in ablated anthers
- Barnase destroys cells for structural tissues
![Page 12: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/12.jpg)
+Pelargonium zonale explant
Soak it in Agrobacterium- Plasmid with T-DNA region that
containsIpt and Barnase gene under the
control of their promoters
Plasmid leaves bacteria, goes into plant cells
DNA chromosome= tumors
Place into Media with Hormones
- Roots- Shoots
Transfer to a pot to fully grow
![Page 13: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/13.jpg)
+RESULTS
pSAG12::ipt transgenic plants
Delayed leaf senscence
Increased branching
Smaller flowers
Intense coloration
Transgenic lines with PsEND1::barnase
No pollen grains in anther structures
![Page 14: + Production of Engineered long-life and male-sterile Pelargonium plants](https://reader035.vdocuments.us/reader035/viewer/2022062421/56649cfd5503460f949cd4ce/html5/thumbnails/14.jpg)
ReferencesBioMed Central Limited (2012, August 29). No more sneezing: Allergen-free house plants. ScienceDaily. Retrieved November 18, 2012, from http://www.sciencedaily.com /releases/2012/08/120831083315.htm BioMed Central Limited (31 August 2012). Production of engineered long-life and male sterile Pelargonium plants. [Research Article] Available at: http://www.biomedcentral.com/1471-2229/12/156 . [Last Accessed 18 November 2012]. Biomedcentral.com (2012) www.biomedcentral.com - Figure. [online] Available at: http://www.biomedcentral.com/1471-2229/12/156/figure/F5 [Accessed: 27 Nov 2012]. Maria Lis-Balchin, (2007). Geranium and Pelargonium: The Genera Geranium and Pelargonium. 1st ed. New York: Taylor & Francis.
Mary Spink. Growing Pelargoniums. [ONLINE] Available at: : http://www.swanlandnurseries.co.uk/culturalnotes.asp. [Last Accessed 18 November 2012]. Scielo.cl (2007) Electronic Journal of Biotechnology - Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal. [online] Available at: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100007 [Accessed: 18 Nov 2012].