© 2013 pearson education, inc. chapter 9: biotechnology and dna technology $100 $200 $300 $400 $500...

© 2013 Pearson Education, Inc.

Chapter 9: Biotechnology and DNA Technology

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BiotechnologyTools of

BiotechnologyRecombinant

DNAApplications of

rDNA

Products of Genetic

Engineering

FINAL ROUND


Topic 1: Biotechnology

$100 Question

Today, chemicals that organisms do NOT naturally make are made by microorganisms and

a. plants.

b. fungi.

c. protozoa.

d. helminths.ANSWER

BACK TO GAME



$100 Answer

Today, chemicals that organisms do NOT naturally make are made by microorganisms and

a. plants.

b. fungi.

c. protozoa.

d. helminths.

BACK TO GAME



$200 Question

When E. coli has the ability to produce the hormone insulin, what is the reason?

a. It picked up the insulin gene from another cell.

b. It is an ancient gene that now has no function.

c. The insulin gene was inserted into it.

d. It needs to regulate its cell glucose level.

BACK TO GAME

ANSWER



$200 Answer

When E. coli has the ability to produce the hormone insulin, what is the reason?

a. It picked up the insulin gene from another cell.

b. It is an ancient gene that now has no function.

c. The insulin gene was inserted into it.

d. It needs to regulate its cell glucose level.

BACK TO GAME



$300 Question

Natural selection is the process

a. of inserting genes into cells by rDNA technology.

b. whereby organisms with traits that make them better adapted to their environment tend to survive and reproduce more than organisms lacking these traits.

c. by which scientists select desirable breeds of animals or strains of plants to cultivate.

d. None of the answers is correct.

BACK TO GAME

ANSWER



$300 Answer

Natural selection is the process

a. of inserting genes into cells by rDNA technology.

b. whereby organisms with traits that make them better adapted to their environment tend to survive and reproduce more than organisms lacking these traits.

c. by which scientists select desirable breeds of animals or strains of plants to cultivate.

d. None of the answers is correct.

BACK TO GAME



$400 Question

In the field of genetic engineering, cDNA is important because it

a. lacks exons.

b. lacks introns.

c. is really RNA.

d. is really reverse transcriptase.

BACK TO GAME

ANSWER



$400 Answer

In the field of genetic engineering, cDNA is important because it

a. lacks exons.

b. lacks introns.

c. is really RNA.

d. is really reverse transcriptase.

BACK TO GAME



$500 Question

The procedure during which cells can take up DNA from the surrounding environment is called

a. electroporation.

b. protoplast fusion.

c. transformation.

d. microinjection.

BACK TO GAME

ANSWER



$500 Answer

The procedure during which cells can take up DNA from the surrounding environment is called

a. electroporation.

b. protoplast fusion.

c. transformation.

d. microinjection.

BACK TO GAME


Which technique amplifies small samples of DNA to quantities that are large enough for analysis?

a. hybridization

b. PCR

c. RFLP

d. DNA fingerprinting

Topic 2: Tools of Biotechnology

$100 Question

BACK TO GAME

ANSWER



$100 Answer

Which technique amplifies small samples of DNA to quantities that are large enough for analysis?

a. hybridization

b. PCR

c. RFLP

d. DNA fingerprinting

BACK TO GAME


Which of the following techniques is used to identify bacteria carrying a specific gene?

a. cloning

b. Western blot

c. transformation

d. DNA probe


$200 Question

BACK TO GAME

ANSWER


Which of the following techniques is used to identify bacteria carrying a specific gene?

a. cloning

b. Western blot

c. transformation

d. DNA probe


$200 Answer

BACK TO GAME


If a restriction enzyme made a staggered cut in DNA, the ends would be termed _____ ends.

a. flat

b. sticky

c. adhesive

d. blunt


$300 Question

BACK TO GAME

ANSWER


If a restriction enzyme made a staggered cut in DNA, the ends would be termed _____ ends.

a. flat

b. sticky

c. adhesive

d. blunt


$300 Answer

BACK TO GAME



$400 Question

BACK TO GAME

ANSWER

A population of genetically identical cellscarrying a desired vector is called

a. a clone.

b. a species.

c. a library.

d. PCR.



$400 Answer

BACK TO GAME

A population of genetically identical cellscarrying a desired vector is called

a. a clone.

b. a species.

c. a library.

d. PCR.



$500 Question

Which of the following methods is especially valuable in the genetic manipulation of plant and algal cells?

a. protoplast fusion

b. viral transduction

c. transformation

d. cloning

BACK TO GAME

ANSWER



$500 Answer

Which of the following methods is especially valuable in the genetic manipulation of plant and algal cells?

a. protoplast fusion

b. viral transduction

c. transformation

d. cloning

BACK TO GAME


Topic 3: Recombinant DNA

$100 Question

Which of the following is NOT a method for inserting foreign DNA into cells?

a. transformation

b. microinjection

c. restriction digestion

d. electroporation

BACK TO GAME

ANSWER



$100 Answer

Which of the following is NOT a method for inserting foreign DNA into cells?

a. transformation

b. microinjection

c. restriction digestion

d. electroporation

BACK TO GAME



$200 Question

Which of the following is a eukaryote used in genetic engineering?

a. Agrobacterium tumefaciens

b. Pseudomonas aeruginosa

c. Saccharomyces cerevisiae

d. Thermus aquaticus

BACK TO GAME

ANSWER



$200 Answer

Which of the following is a eukaryote used in genetic engineering?

a. Agrobacterium tumefaciens

b. Pseudomonas aeruginosa

c. Saccharomyces cerevisiae

d. Thermus aquaticus

BACK TO GAME


What are the two main sources of DNA that scientists use to obtain the genes in which they are interested?

a. genomic libraries and PCR

b. synthetic DNA and plasmids

c. synthetic DNA and genomic libraries

d. PCR and plasmids


$300 Question

BACK TO GAME

ANSWER



$300 Answer

What are the two main sources of DNA that scientists use to obtain the genes in which they are interested?

a. genomic libraries and PCR

b. synthetic DNA and plasmids

c. synthetic DNA and genomic libraries

d. PCR and plasmids

BACK TO GAME



$400 Question

Restriction enzymes are

a. animal enzymes that splice RNA.

b. bacterial enzymes that destroy phage DNA.

c. animal enzymes that destroy RNA.

d. viral enzymes that destroy host DNA.

BACK TO GAME

ANSWER



$400 Answer

BACK TO GAME

Restriction enzymes are

a. animal enzymes that splice RNA.

b. bacterial enzymes that destroy phage DNA.

c. animal enzymes that destroy RNA.

d. viral enzymes that destroy host DNA.



$500 Question

Which enzyme is used to synthesize cDNA?

a. RNA polymerase

b. complementary polymerase

c. reverse transcriptase

d. DNA polymerase

BACK TO GAME

ANSWER



$500 Answer

Which enzyme is used to synthesize cDNA?

a. RNA polymerase

b. complementary polymerase

c. reverse transcriptase

d. DNA polymerase

BACK TO GAME


Topic 4: Applications of rDNA

$100 Question

In plants, the Ti plasmid can cause

a. crown gall disease.

b. tobacco mosaic disease.

c. Pseudomonas.

d. soft rot disease.

BACK TO GAME

ANSWER



$100 Answer

In plants, the Ti plasmid can cause

a. crown gall disease.

b. tobacco mosaic disease.

c. Pseudomonas.

d. soft rot disease.

BACK TO GAME



$200 Question

Molecule-sized robots used to detect contamination in food are an example of

a. forensic microbiology.

b. nanotechnology.

c. biotechnology.

d. bioinformatics.

ANSWER

BACK TO GAME



$200 Answer

Molecule-sized robots used to detectcontamination in food are an example of

a. forensic microbiology.

b. nanotechnology.

c. biotechnology.

d. bioinformatics.

BACK TO GAME



$300 Question

What can be used to screen a sample for multiple pathogens simultaneously?

a. DNA chips

b. Southern blotting

c. shotgun sequencing

d. colony hybridization

BACK TO GAME

ANSWER



$300 Answer

What can be used to screen a sample for multiple pathogens simultaneously?

a. DNA chips

b. Southern blotting

c. shotgun sequencing

d. colony hybridization

BACK TO GAME



$400 Question

When mammalian cells are used to make protein products in genetic engineering, there is a(n)

a. high risk of toxin production.

b. increase in allergic reactions.

c. low risk of toxin production.

d. low rate of protein production.

BACK TO GAME

ANSWER



$400 Answer

BACK TO GAME

When mammalian cells are used to make protein products in genetic engineering, there is a(n)

a. high risk of toxin production.

b. increase in allergic reactions.

c. low risk of toxin production.

d. low rate of protein production.



$500 Question

Which bacterium has been genetically modified to produce a human protein, gamma interferon?

a. Bacillus thuringiensis

b. Staphylococcus aureus

c. Escherichia coli

d. Pseudomonas fluorescens

BACK TO GAME

ANSWER



$500 Answer

Which bacterium has been genetically modified to produce a human protein, gamma interferon?

a. Bacillus thuringiensis

b. Staphylococcus aureus

c. Escherichia coli

d. Pseudomonas fluorescens

BACK TO GAME


Topic 5: Products of Genetic Engineering

$100 Question

The Ti plasmid naturally occurs in

a. Thermus aquaticus.

b. Saccharomyces cerevisiae.

c. Bacillus thuringiensis.

d. Agrobacterium tumefaciens.

BACK TO GAME

ANSWER



$100 Answer

The Ti plasmid naturally occurs in

a. Thermus aquaticus.

b. Saccharomyces cerevisiae.

c. Bacillus thuringiensis.

d. Agrobacterium tumefaciens.

BACK TO GAME



$200 Question

The method of using RFLPs to identify bacterial or viral pathogens is called

a. proteomics.

b. DNA fingerprinting.

c. genetic screening.

d. DNA sequencing.

BACK TO GAME

ANSWER



$200 Answer

The method of using RFLPs to identifybacterial or viral pathogens is called

a. proteomics.

b. DNA fingerprinting.

c. genetic screening.

d. DNA sequencing.

BACK TO GAME



$300 Question

One of the earliest commercial products of rDNA technology was

a. rennin.

b. cellulase.

c. malaria vaccine.

d. human growth hormone.

BACK TO GAME

ANSWER



$300 Answer

BACK TO GAME

One of the earliest commercial products of rDNA technology was

a. rennin.

b. cellulase.

c. malaria vaccine.

d. human growth hormone.



$400 Question

Which of the following is NOT a therapeutic application of biotechnology?

a. creating herbicide-resistant plants

b. producing DNA vaccines

c. gene therapy

d. synthesizing subunit vaccines

BACK TO GAME

ANSWER



$400 Answer

Which of the following is NOT a therapeutic application of biotechnology?

a. creating herbicide-resistant plants

b. producing DNA vaccines

c. gene therapy

d. synthesizing subunit vaccines

BACK TO GAME



$500 Question

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

a. inserting the Ti into Agrobacterium.

b. transformation of an animal cell.

c. transformation of E. coli with Ti.

d. splicing Ti into a plasmid.

BACK TO GAME

ANSWER



$500 Answer

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

a. inserting the Ti into Agrobacterium.

b. transformation of an animal cell.

c. transformation of E. coli with Ti.

d. splicing Ti into a plasmid.

BACK TO GAME


FINAL ROUND Question

One surprising finding in the Human Genome Project was that “junk DNA,” which does NOT code for a functional product, makes up what percentage of the human genome?

a. 90%

b. 95%

c. 98%

d. 85%

BACK TO GAME

ANSWER


FINAL ROUND Answer

One surprising finding in the Human GenomeProject was that “junk DNA,” which does NOTcode for a functional product, makes up whatpercentage of the human genome?

a. 90%

b. 95%

c. 98%

d. 85%

BACK TO GAME

© 2013 pearson education, inc. chapter 9: biotechnology and dna technology $100 $200 $300 $400 $500...

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