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Are there Microbes Lurking?

June 12, 2012 | State University of NY

Paul W. Tranchell CIH, CSP, RBPProject Manager

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Are there Microbes Lurking?

June 12, 2012 | State University of NY

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Occupational Stressors: (i.e. biological agents)

Anticipation

Recognition

Evaluation

Control

Industrial Hygiene

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Definition

Normal Locations

Selected Suppliers

Unusual & Ancillary Biohazards

Biosafety Fundamentals

Agenda

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Biosafety

The application of knowledge, techniques and equipment to prevent personal, laboratory, and environmental exposure to potentially infectious agents or biohazards

Biosafety defines the containment conditions under which infectious agents can be safely manipulated

The objective of containment is to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents

Medicine.net http://www.medterms.com/script/main/art.asp?articlekey=33817

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Normal Locations

Bloodborne Pathogens

Microbiological Laboratories

Animal Laboratories

Molecular Biology Labs

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Select Suppliers

American Type Culture Collection (ATCC) - Repository of a huge number of cell lines

CABRI (Common Access to Biological Resources and Information)

Developmental Studies Hybridoma Bank - Monoclonal antibodies

DSMZ - The DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH = German Collection of Microorganisms and Cell Cultures) is a non-profit public domain general repository specializing in tumor cell lines. It holds the largest collection of human hematopoietic cell lines currently available worldwide.

European Collection of Cell Cultures (ECACC) - ECACC "was established in 1984 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe."

HyperCLDB - Hypertext resource derived from the Cell Line Data Base (CLDB) with descriptions of more than 4,000 cell lines

National Cell Culture Center - A "national resource facility established by the National Institutes of Health (NIH) to provide customized, large scale, cell culture services for basic research laboratories."

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Unusual & Ancillary Hazards

Field Work Lyme Disease Insect Bites Suborder Surpentes Rodent Diseases

› Plague› Hanta Virus

HVAC / Legionella

Bird Droppings (no bleach please)

Mold

Suspicious Packages / ”white” powder

Drum Skins

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Chain of Infection

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Biosafety Fundamentals

Risk Assessment

Reservoir of

pathogen

Portal of

escapeTransmission

Route of entry /

infectious dose

Susceptible host

Incubation period

Practices / Equipment PPE Access control /

Immunization SurveillanceFacilities

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Definitions

Biosafety Level Risk Guide NIH

1

well characterized agents not consistently known to cause disease in healthy adult humans of minimal potential hazard to laboratory personnel and the environment

Agents that are not associated with disease in healthy adult humans. Includes a list of animal viral etiologic agents in common use.

2

agents of moderate potential hazard to personnel and the environment

Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available.

3

indigenous or exotic agents which may cause serious or potentially lethal disease as a result of exposure by the inhalation route

Agents that are associated with serious or lethal human disease for which preventive or therapeutic interventions may be available

4

dangerous and exotic agents which pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease

Agents that are likely to cause serious or lethal human disease for which preventive or therapeutic interventions are not usually available

Biosafety Fundamentals

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Control Strategies

Standard Microbiological Practices

Special Microbiological Practices

Safety Equipment Primary Barrier

› Biological Safety Cabinets (BSC)› Types, Selection, Use!

Personal Protective Equipment

Laboratory Facilities Secondary Barriers

Biosafety Fundamentals

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QUESTIONS?

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