© 2007 msa msa biosensor biological agent detector
TRANSCRIPT
© 2007 MSA
MSA BIOSENSOR™MSA BIOSENSOR™
Biological Agent Biological Agent DetectorDetector
© 2007 MSA
ProblemProblem
Major Bio-Threats Anthrax Ricin SEB (staph infection) Botulism Plague Cholera Tularemia Smallpox
© 2007 MSA
Homeland DefenseHomeland Defense
Personnel responsible: First Responders Fire Departments Police Departments HazMat teams Hospital Triage Military F.B.I.
© 2007 MSA
SolutionSolution
Needs for Homeland Defense: On-site/portable analysis Rapid detection Easy to use Excellent sensitivity Low false positives Test multiple targets at same time
© 2007 MSA
Handheld Detector forBiological Agents
Handheld Detector forBiological Agents
MSA BIOSENSOR™MSA BIOSENSOR™Biological Agent DetectorBiological Agent Detector
© 2007 MSA
Product PerformanceProduct Performance
B. Anthracis sensitivity: <10,000 spores
Ricin sensitivity:
0.5 ngFalse positives:
< 1 in 1,000,000Time to answer:
Results in 5 minutes
© 2007 MSA
Product PerformanceProduct PerformanceThe MSA BIOSENSOR 2200R Biological Agent Detector has an established track record in real-world applications and has been subject to successful third-party testing.
© 2007 MSA
MSA BIOSENSOR 2200R DetectorMSA BIOSENSOR 2200R Detector Handheld Dimensions
11”(L) x 10”(W) x 5”(H) Weight: 7 pounds Battery life: 50 + tests LCD screen 4 button user interface Integrated RFID Data storage for up to 50 test results Serial port for data download Training time: 1 hour
© 2007 MSA
CartridgeCartridge
Single-useSelf-sealingReagents in cartridgeShelf life:12 monthsStorage conditions: room temperatureRFID for automatic cartridge identification
Available Targets•Anthrax •Ricin•SEB (staph infection)
© 2007 MSA
CartridgeCartridge
Detection Chamber
© 2007 MSA
CartridgeCartridge
Mixing Ball
© 2007 MSA
CartridgeCartridge
Magnetic Microsphere with specific Biological antibodies (Mag)
© 2007 MSA
CartridgeCartridge
Fluorescent Tag with specific Biological antibodies (Tag)
© 2007 MSA
Ease of UseEase of Use Operation
1. Insert wash syringe into cartridge
2. Collect sample and draw into sample syringe
3. Insert sample syringe into cartridge, push on plunger
4. Shake cartridge for one minute
5. Insert into instrument
6. Wait 3 minutes, then push down on wash syringe
7. Push “READ” button, obtain easy-to-interpret results:
RED LIGHT – TARGET PRESENT
GREEN LIGHT – NO TARGET PRESENT
© 2007 MSA
BIOSENSOR Assay FormatBIOSENSOR Assay Format
Dynamic surface generation and detection Combines benefits of free solution and lateral flow type assays Concentration increases sensitivity Wash step improves assay robustness Compatible with continuous monitor system
MIX BIND/MAGNETIZE
WASH READ
© 2007 MSA
BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology
1. MIX Sample is mixed with sensing solution: Magnetic microspheres and fluorescent tag.
© 2007 MSA
BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology
2. BIND Sensing materials bind spores during
mixing and incubation.
© 2007 MSA
BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology
3. MAGNETIZE All bound and unbound magnetic
material separate to surface.
© 2007 MSA
BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology
4.WASH Unbound TAG remains in solution
and is washed away.
© 2007 MSA
BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology
5. READ Concentrated sample (pellet) is illuminated
and emits a signal if target is present.
© 2007 MSA
< 10,000 spores easily detected within five minutes.
Anthrax (bacillus anthracis) Limit of DetectionAnthrax (bacillus anthracis) Limit of Detection
© 2007 MSA
Ricin (ricinus communis) Limit of DetectionRicin (ricinus communis) Limit of Detection
< 1 ng detected within five minutes.
© 2007 MSA
Anthrax/Ricin Multiplex Limit of DetectionAnthrax/Ricin Multiplex Limit of Detection
< 10,000 BA spores easily detected within 5 minutes.
< .5 Ricin ng detected within 5 minutes.
© 2007 MSA
Anthrax (bacillus anthracis) Interference StudyAnthrax (bacillus anthracis) Interference Study
No false positives from common interferents
© 2007 MSA
Anthrax Spores Interference Study Anthrax Spores Interference Study 0.1 mg/mL of the indicated powders were mixed with 5000 Pasteur spores and analyzed on the Anthrax specific cartridges.
Added Powder (0.1mg/mL) Response (mV) Result
None (neat Pasteur spike) 312 - 573 True Positive
Spackling Powder 179 True Positive
Baking Soda (Arm & Hammer) 934 True Positive
Instant Non-fat Dried Milk (Nestle Carnation) 652 True Positive
Talcum Powder (Equate) 453 True Positive
Flour (Gold Medal) 499 True Positive
Salt (Morton) 550 True Positive
Yeast (Red Star) 609 True Positive
Powdered Sugar (Confectioners) 294 True Positive
Dipel™ (Abbot Laboratories) 238 True Positive
Chalk (Bison) 272 True Positive
Foot Powder (Total Body) 522 True Positive
Ajax™ with Bleach (Colgate-Palmolive) 213 True Positive
Non-fat Dairy Creamer (Great Value) 520 True Positive
Kaolin (Fisher-Scientific) 528 True Positive
Bentonite (Aldrich) 356 True Positive
Aerosil R812S™ (Degussa) 485 True Positive
© 2007 MSA
Ricin (A Chain) Assay Interference Study Ricin (A Chain) Assay Interference Study
No false positives from common interferents.
0
50
100
150
200
250
300
RF
U
Ricin Assay Interference StudyInterferent concentration = 100 ug/mL
© 2007 MSA
Other Bacillus Spores Interference Study Other Bacillus Spores Interference Study
No false positives from nearest neighbor spores.
© 2007 MSA
MSA BIOSENSOR 2200R Detector PlatformMSA BIOSENSOR 2200R Detector Platform
On-site/portable analysis Rapid detection Excellent sensitivity Extremely easy to use Excellent protection against false positives Single test capabilities for multiple bio-threats
(anthrax/ricin combo)
© 2007 MSA
Target AvailabilityTarget Availability Available Targets
Anthrax/ricin multi-agentAnthrax Ricin SEB (staph infection)
Under DevelopmentBotulinumPlagueCholeraTularemiaSmallpoxWest Nile virusOther agents in
development
© 2007 MSA
BIOSENSOR 2200R Biological Agent DetectorCompetitive Comparison
BIOSENSOR 2200R Biological Agent DetectorCompetitive Comparison
© 2007 MSA
Ordering InformationOrdering Information MSA BIOSENSOR 2200R Detector Kit (10084834) Includes:
● BIOSENSOR 2200R Detector ● Cartridge starter kit:
-3 Anthrax/ricin combo
-3 Positive control-1 Anthrax-1 Ricin-2 Negative control
● Battery charger ● Instruction manual ● Laminated quick instruction guide ● Carrying bag for all contents
© 2007 MSA
Cartridge Replacement PlansCartridge Replacement Plans
Plans based upon expected annual white powder calls
Low (6 per year) - 10084753 Medium (2 per month) - 10084754 High (5 per month) - 10084755
© 2007 MSA
Cartridge Replacement PlansCartridge Replacement Plans
Low (1 every 2 months) Includes:
6 Anthrax/Ricin Combo 6 Positive Control 2 Anthrax 2 Ricin 2 Negative Control 2 Wet sample kits 2 Calibration kits
Includes 2 positive controls
All biohazard targets kits include dry sample kit
© 2007 MSA
Cartridge Replacement PlansCartridge Replacement Plans
Medium (2 per month) Includes:
24 Anthrax/Ricin Combo 24 Positive Control 6 Anthrax 6 Ricin 6 Negative Control 4 Wet sample kits 2 Calibration kits
Includes 2 positive controls
All biohazard targets kits include dry sample kit
© 2007 MSA
Cartridge Replacement PlansCartridge Replacement Plans
High (5 per month) Includes:
60 Anthrax/Ricin Combo 60 Positive Control 8 Anthrax 8 Ricin 8 Negative Control 6 Wet sample kits 2 Calibration kits
Includes 2 positive controls
All biohazard targets kits include dry sample kit
© 2007 MSA
MSA BIOSENSOR™MSA BIOSENSOR™Biological Agent DetectorBiological Agent Detector