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7070

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etcetcarthropodarthropod--borne virusborne virus

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WN KunMVE JE SLEMVE JE SLE

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22.3.

4.5.

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37

106~7 pfu/mLP/N P/N

NS1IgMIgM

IgG,

0 1 2 3 4 5 6 7 8 9 103 2 1

2015/7/28

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100,000/mm100,000/mm33

12 20

LL LR

R RL

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/

(DHF grade 1, 2) DHF grade 3,4)

y 0.18% 0.007%

y 2.01% 1.14%

EnhancementEnhancement

FcFc

FcFc

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6

•

•

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Culex tritaeniorhynchusCulex tritaeniorhynchus

km

27

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2015/7/28

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19431

1 Dengue virus type 1, Mochizuki strain

•

•

•humanized

NOD/SCID/interleukin 2 receptor gamma (IL-2R )-

null (hu-NSG) mice

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1942 45

458,000 4,219,000 306,000 582,0001942 50,000 5,000 No epidemic No epidemic

1943 20,000 10,000 200 No epidemic

1944 5,000 100,000 100 2001944 , ,1945 Unknown 3,000 No epidemic Unknown1946 No epidemic No epidemic No epidemic No epidemic

Susumu Hotta. Dengue epidemics in Japan, 1942-1945. J. Trop. Med. Hygiene. 56: 83. 1953.

102006 13 1005

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2015 6 5

20152015

23

21

25

21

10

15

20

6

3

0

5

1 2 3 4

23

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1818

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2013

2015

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•1946 ’47 Mosquito Fauna of Japan & Korea. By Walter J. LaCasse & Satyu Yamaguchi)

•1970•1990 20km

Kurihara et al. 1997

20352100 !

1198%

Dengue endemic areaDengue endemic area

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C6/36

2013 9 2014 1

day 15 8/19

day 15~ 13 8/19~21

day 13~ 10 8/21~24

51

*day 10~ 9 8/24~25

day 9~ 6 8/25~28

day 6~ 3 8/28~31

day 3 8/31

day 0 9/3 40

day 6 9/9IgG (IFA): 1:20,480 ,IgM (IFA): 1:320NS1 (ELISA): , RT PCR:

2

Eurosurveillance, Volume 19, Issue 3, 23 January 2014 (* ProMed

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17

8/28 5-8/29

9/4A

121

8/27

4

6

8

10n=122

n=8

n=21

2014 10 6 11

0

2

Yoyogi story

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5

6

7

9/4A

2014 8 1 9 30

n=70 122 1

0

1

2

3

4

4n=5 8

0

1

2

3

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

75 72 6 ( 2 132014 10 6 11

19

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10 4 1 5

1021410 4 1 5

10 14 16 30

20 27 26 53

30 11 7 18

40 12 11 23

50 11 2 13

1273322211

60 8 0 8

70 4 1 5

91 64 155

1111111

156

2014 10 6 11

2013 2014

2012 As of

1,762 0 1.1(1,541) 1,514 NA1,762 0 ( , ) 1,514 NA

16,722 53 0.4(40,164) 3,543 21

44,171 95 4.4 (9,952) 1,716 0

43,346 83 2.0 (21,900) 108,698 215

166,107 528 1.0 (170,693) 104,741 39922,194 5 4.8 (4,632) 18,318 460,588 38 0.8 (79,485) 17,766 17

581 0 9/3 45,171 NA

14 NA

10,541 7 26.0 9/14

6,591 8 NA 7/17

WHO

26,067 33 3.3 4/30

869 NA 15,904 20

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1 41 4

CYD TDV 3 56.5%60.8%( )( )

DENVax 2 a

TetraVax DV 2 a

TDENV PIV 1 aTDENV PIV 1

DNA TVDV 1 a

DEN V180 1 a

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Construction of CYD TDY; Sanofi Pasteur

C prM E NS1 NS2A

NS2B

NS3

NS4A

NS4B

5’ NTR 3’ NTR

0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000

NS5

EM &RNA transfection in Vero cells

EprM &

D4

EprM &

EprM &

D1

D3

D2

EprM &

4 chimeric cDNAs

Individually transcribed to RNA

Exchange with prM/Egenes of wt DEN1 4

D1

D4

D2

D3

4 individualchimericDengue viruses(CYD 1 4)

2

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• 3–7 3 12••

•

•

• ( 65 years),

•

Bone scintigraphy

The wrists & hands showing an intense focus, particularly on the left side.

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Hochedez et al.EID 12(10), 2006.

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11

12

CDC

*

CDC

4 IGR

5 CDC

CDCCDC 88

48

7~8

8

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• DEET

•

• DEET

DEET TCDD !!