-11.9 kb -4.2 kb -5.6 kb gfi1 +/1b neo gfi1 +/+ gfi1 +/1b gfi1 1b/1b gfi1 +/1b gfi1 +/+ -11.9 kb -...
DESCRIPTION
∆ Rn cycle Amplification Plot Gfi1 1b/1b E E E E E+1 Gfi1 Gfi1b E E E E E+1 Gfi1 Gfi1b Amplification Plot Gfi1 +/+ A B Gfi1 Gfi1b GAPDH Gfi1 +/+ Gfi1 1b/1b H 2 O Supp. Figure 3TRANSCRIPT
-11.9 kb
-4.2 kb
-5.6 kb
Gfi1+/1b neo
Gfi1+/+
Gfi1+/1b
Gfi11b/1b
Gfi1+/1b
Gfi1+/+
-11.9 kb
- 4.2 kb
Gfi1+/+
Gfi1+/1b
-14 kb
- 8 kb
BAGfi1+/+ Gfi1+/1b Gfi11b/1b
Gfi1(55 kDa)
Gfi1b(37 kDa)
loading control
C
loading control
Supp. Figure 1
Gfi1+/+
Gfi1P2A/P2A
- 15 kb
- 8 kb
BGfi1
+/P2A
Gfi1+/+
- 15 kb
- 8 kb
Gfi1+/P2A
Gfi1P2A/P2A
Gfi1+/+
Gfi1-/-
Gfi1(55kDa)
loading control
A
Supp. Figure 2
∆ Rn
cycle
0 5 10 15 20 25 30 35 40
Amplification Plot Gfi11b/1b
1.000 E-4
1.000 E-3
1.000 E-2
1.000 E-1
1.000
1.000 E+1
Gfi1
Gfi1b
1.000 E-4
1.000 E-3
1.000 E-2
1.000 E-1
1.000
1.000 E+1
Gfi1Gfi1b
0 5 10 15 20 25 30 35 40
Amplification Plot Gfi1+/+
A
B
Gfi1
Gfi1b
GAPDH
Gfi1+/+ Gfi11b/1b H2O
Supp. Figure 3
IP: anti Gfi1bWe: anti PIAS3
PIAS3
175
8362
47.5
IP: anti Gfi1bWe: anti Gfi1b
Inpu
t
IP a
nti-G
ST
IP a
nti-G
fi1b
Gfi1b
IgG
8362
47.5
32.5
Supp. Figure 4
Table 1 Differential cell counts of wild type, Gfi1-/- , Gfi1P2A/P2A and Gfi11b/1b mice
Supp. Table 1
significant
bone marrow
cell counts (%)Gfi1+/+
(n=7)
Gfi1-/-
(n=8)
t-test Gfi11b/1b
(n=7)
t-test Gfi1P2A/P2A
(n=3)
t-test
lymphocytes 22 ± 11 7 ± 7 0.01∗ 19 ± 8 0.617 6 ± 3 0.055
m yeloblasts 4 ± 2 4 ± 5 1 7 ± 3 0.063 9 ± 8 0.214
prom yeloblasts 4 ± 2 2 ± 2 0.075 5 ± 2 0.56 4 ± 5 0.902
m yelocytes 7 ± 4 4 ± 2 0.06 13 ± 6 0.053 9 ± 7 0.622
m etam yelozytes 20 ± 7 4 ± 2 4.1x10-5 ∗ 13 ± 4 0.049 ∗ 4 ± 1 0.006 ∗
granulocytes 36 ± 13 2± 3 5.4x10-6 ∗ 28 ± 7 0.202 2 ± 2 0.002 ∗
m onocytes 2 ± 2 16 ± 24 0.173 7 ± 4 0.014 ∗ 13 ± 1 2.7x10-5 ∗
atypical m onocytes 1 ± 3 57 ± 21 1.1x10-5 ∗ 2 ± 6 0.688 48 ± 24 0.002 ∗
eosinophils 2 ± 2 1 ± 1 0.449 1 ± 1 0.446 1 ± 1 0.705
erythroid 3 ± 5 3± 3 0.75 5 ± 6 0.484 3 ± 4 0.906
blood
cell counts (%)
Gfi1+/+
(n=9)
Gfi1-/-
(n=5)
t-test Gfi11b/1b
(n=7)
t-test Gfi1P2A/P2A
(n=3)
t-test
lym phocytes 80 ± 6 54 ± 22 0.005 ∗ 81 ± 9 0.802 51 ± 21 0.002 ∗
granulocytes 15 ± 6 0 ± 1 9.1x10-5 ∗ 10 ± 5 0.067 2 ± 3 0.003 ∗
m onocytes 4 ± 2 24 ± 22 0.016 ∗ 8 ± 5 0.046 ∗ 23 ± 18 0.005 ∗
atypical m onocytes 0 20 ± 8 6.5x10-6 ∗ 0 ± 1 0.121 23± 7 3.5x10-7 ∗
eosinophils 1 ± 1 1 ± 2 0.979 1 ± 1 0.749 0 ± 1 0.32